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The map label for this gene is menE [H]

Identifier: 42522148

GI number: 42522148

Start: 509592

End: 510665

Strand: Direct

Name: menE [H]

Synonym: Bd0548

Alternate gene names: 42522148

Gene position: 509592-510665 (Clockwise)

Preceding gene: 42522147

Following gene: 42522149

Centisome position: 13.47

GC content: 51.3

Gene sequence:

>1074_bases
TTGTACCGTCTTGCGGAAGAAAGTCAGATTGAAAAAAACCTGAAAGGCCATGTGTGGATCGCAACATCCGGTTCCACCGC
GAATTCGATTTCGGCGACCAAGCTTGTGGCTTTATCGAAGCAGGCGCTGCTGGCATCGGCTCGGGCGGTGAATCTTCATC
TGCAAAGTTCTGCTAAAGATGTGTGGACTCAGGTGCTGCCGCATTTTCATGTTGGGGGCCTGGGTATAGAAATCCGCGCG
CATCTTAGCGGAGCCAAGGTTGTGAAGGCCCTGAAAGACGGGCGCTGGGACGTTCAGTATTTTTATGACGTGCTTGTTTC
TGAAGGTTGTACTTTGTCGGCCCTGGTGCCCACGCAAGTGTATGATCTGGTGTCGCACGGCTTAAGGGCGCCAGCGACTT
TGCGTGCGGTCGTGGTTGGTGGCGGAGCCTTTGAGGTGGATCTTTACAAAAAAGCCCGCGCACTCGGATGGCCAGTTCTT
CCCAGTTATGGGATGAGCGAAACGGCCTCGCAGATTGCCACGGCGTCTTTGGACAGTCTGAATCAGGATGAATTTCCGCC
CGTGGGGCTTTTGACTCACGCCAAAGCCCGGCAGAATGCTGGTGGCTATTTGGAAGTATGGGCAGATTCTTTATTTACTT
GTTATGCCCAGAACACGGAATCCGGTTCCCGTATCTGGAATCCCAAAACGGCCGACGGATGGTTTACGACGGAAGACAAA
GGTTCGGTCGTGAATGGCAGTCTGCTGATTGAAGGCCGCAGCAAAGACTATGTCAAGATTGGTGGCGAAGCGACCAATGT
GGCAAGACTTCGTTCTGTTCTTGAATCCTGCGCTTTGCATTTGAATCCGCACTGGCCGACTCAGGTGACGTTGTTGGATG
TGCCTTCTGACAGACTGGGGGCTGAGATTCATCTGGTCAGTCTTTTGTCGGAAGCTGACACAGACAAAGTCCTGAAGTTG
TATTCAGAAAAAGTGCTTCCCTTTGAAAAAGCACGTAAAATTTATTATATGAACGAGATCCCACGCAGTGATTTGGGAAA
AATTCTGTGGGCGGAGCTGAGGAGAAAACTATGA

Upstream 100 bases:

>100_bases
AGGCCCTGACGTGGTATCAGCTCAAAGTCCGATAGATCTTCATTCTGAATCTAACGAAATCCTGCTGAACCCGCGCTGGC
CTGAAATCGACTATCAGGCT

Downstream 100 bases:

>100_bases
AGCACGAAGAAAACCCCTGGTCCAAAGGCATCGTTCTGATCTGCACGAAATGCCATAAATCCATCAGCCCCAAACTGCTG
GAAGAAGAAGGCAACAGTGC

Product: menE; O-succinylbenzoate-CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MYRLAEESQIEKNLKGHVWIATSGSTANSISATKLVALSKQALLASARAVNLHLQSSAKDVWTQVLPHFHVGGLGIEIRA
HLSGAKVVKALKDGRWDVQYFYDVLVSEGCTLSALVPTQVYDLVSHGLRAPATLRAVVVGGGAFEVDLYKKARALGWPVL
PSYGMSETASQIATASLDSLNQDEFPPVGLLTHAKARQNAGGYLEVWADSLFTCYAQNTESGSRIWNPKTADGWFTTEDK
GSVVNGSLLIEGRSKDYVKIGGEATNVARLRSVLESCALHLNPHWPTQVTLLDVPSDRLGAEIHLVSLLSEADTDKVLKL
YSEKVLPFEKARKIYYMNEIPRSDLGKILWAELRRKL

Sequences:

>Translated_357_residues
MYRLAEESQIEKNLKGHVWIATSGSTANSISATKLVALSKQALLASARAVNLHLQSSAKDVWTQVLPHFHVGGLGIEIRA
HLSGAKVVKALKDGRWDVQYFYDVLVSEGCTLSALVPTQVYDLVSHGLRAPATLRAVVVGGGAFEVDLYKKARALGWPVL
PSYGMSETASQIATASLDSLNQDEFPPVGLLTHAKARQNAGGYLEVWADSLFTCYAQNTESGSRIWNPKTADGWFTTEDK
GSVVNGSLLIEGRSKDYVKIGGEATNVARLRSVLESCALHLNPHWPTQVTLLDVPSDRLGAEIHLVSLLSEADTDKVLKL
YSEKVLPFEKARKIYYMNEIPRSDLGKILWAELRRKL
>Mature_357_residues
MYRLAEESQIEKNLKGHVWIATSGSTANSISATKLVALSKQALLASARAVNLHLQSSAKDVWTQVLPHFHVGGLGIEIRA
HLSGAKVVKALKDGRWDVQYFYDVLVSEGCTLSALVPTQVYDLVSHGLRAPATLRAVVVGGGAFEVDLYKKARALGWPVL
PSYGMSETASQIATASLDSLNQDEFPPVGLLTHAKARQNAGGYLEVWADSLFTCYAQNTESGSRIWNPKTADGWFTTEDK
GSVVNGSLLIEGRSKDYVKIGGEATNVARLRSVLESCALHLNPHWPTQVTLLDVPSDRLGAEIHLVSLLSEADTDKVLKL
YSEKVLPFEKARKIYYMNEIPRSDLGKILWAELRRKL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=255, Percent_Identity=32.9411764705882, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=349, Percent_Identity=25.7879656160458, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 39110; Mature: 39110

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLAEESQIEKNLKGHVWIATSGSTANSISATKLVALSKQALLASARAVNLHLQSSAKD
CCCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHH
VWTQVLPHFHVGGLGIEIRAHLSGAKVVKALKDGRWDVQYFYDVLVSEGCTLSALVPTQV
HHHHHCCCCEECCEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEHHHHH
YDLVSHGLRAPATLRAVVVGGGAFEVDLYKKARALGWPVLPSYGMSETASQIATASLDSL
HHHHHCCCCCCCEEEEEEEECCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
NQDEFPPVGLLTHAKARQNAGGYLEVWADSLFTCYAQNTESGSRIWNPKTADGWFTTEDK
CCCCCCCCCCEECCHHHCCCCCEEEEHHHHHEEEEECCCCCCCEECCCCCCCCEEEECCC
GSVVNGSLLIEGRSKDYVKIGGEATNVARLRSVLESCALHLNPHWPTQVTLLDVPSDRLG
CCEEECEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCHHHC
AEIHLVSLLSEADTDKVLKLYSEKVLPFEKARKIYYMNEIPRSDLGKILWAELRRKL
CEEHHHHHHHHCCHHHHHHHHHHHCCCHHHHCEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYRLAEESQIEKNLKGHVWIATSGSTANSISATKLVALSKQALLASARAVNLHLQSSAKD
CCCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHH
VWTQVLPHFHVGGLGIEIRAHLSGAKVVKALKDGRWDVQYFYDVLVSEGCTLSALVPTQV
HHHHHCCCCEECCEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEHHHHH
YDLVSHGLRAPATLRAVVVGGGAFEVDLYKKARALGWPVLPSYGMSETASQIATASLDSL
HHHHHCCCCCCCEEEEEEEECCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
NQDEFPPVGLLTHAKARQNAGGYLEVWADSLFTCYAQNTESGSRIWNPKTADGWFTTEDK
CCCCCCCCCCEECCHHHCCCCCEEEEHHHHHEEEEECCCCCCCEECCCCCCCCEEEECCC
GSVVNGSLLIEGRSKDYVKIGGEATNVARLRSVLESCALHLNPHWPTQVTLLDVPSDRLG
CCEEECEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCHHHC
AEIHLVSLLSEADTDKVLKLYSEKVLPFEKARKIYYMNEIPRSDLGKILWAELRRKL
CEEHHHHHHHHCCHHHHHHHHHHHCCCHHHHCEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]