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The map label for this gene is rpoD [H]

Identifier: 42521867

GI number: 42521867

Start: 228010

End: 229839

Strand: Direct

Name: rpoD [H]

Synonym: Bd0242

Alternate gene names: 42521867

Gene position: 228010-229839 (Clockwise)

Preceding gene: 42521866

Following gene: 42521868

Centisome position: 6.03

GC content: 47.98

Gene sequence:

>1830_bases
ATGAAACCGAACCTACCAAATAAACAAGAGAAAGAGCCTGTGAAGGTTCTTTCTTTGCAGGAACAGGAAACTCTGATTAA
AGAAGAAATCCAGAGATTCCTGAAGCTTGCAAAGGAGAAGGGTGCACTGACCATTGAGGAAATCAATGAGCTCCTTCCCC
CTGAAATTGTCGCTCCATCTGTGCTGGACTCGTTCATGCAGGGCCTTGAGGCCGGTGGTGTAGTAATTTCAGACTTGTCT
GAAAGTTCGAAAGAGGAAGAGGGCGAAGGCGCCTTCCTGGAAAACGTCGATTCTGAAGACGAAGAAATTGATGATGAAGA
AAAAGCGGAAAGTGAAGACGTTAAGGGTAACGACCCTGTACGCCTTTACCTGCGCAAAATGGGAAGCGTTTCCCTGCTGA
CCCGTGAAGGGGAAGTGGAAATTGCCCGTCGTATTGAAAAAGGGGAGCGCGAGATCGTTCGTGCGATCCTTTTGTCCCCT
CTGGGTACCTACGAGATCATCCAGCTTGGTAAGCGCCTTGATGAAGGCCGTATCAAAGTTAAATCGATCTTCCGCGGTCT
TGAGGACGAGGACACCCAGTACGATGAAAAGGAATACATCGATAAGATCCATGAGCTTATCGGTCGTGTGACTGAGTACC
AGAAAGCGGCGGAAAAACTGTTTATGACTCTTCGTAAAGAAGAGACAAACACTCCGGCTCGTCAGGCTGCTATGAAAGAG
CTTGTTGCCATGAATGACAAGTTGATGGCGAACTTCGAGTCCGTGAATTTCAACCGTAAAACTATCAACCGCGTTGTGAT
CAAGTTCAAAAACCTGGTGAACCGTATCGGAACTCTTCGTCGTCGTATCAAAGACGGTGTGGAGCGTACGTTCTCTAAAG
ACGTCACAGCGATGGCTGAACGTATGAAGTTGATCGATGCGAATGAAAAAGAACTGACCCGTATGACCCGCGATACTGGT
TTGAACTATCAGAAGTTCAAATCCTACGTATTGCAGGCTCAGGAAGCGGAAAAGCGTATCAGCCGTTTGGATTCAGAAAC
TGAAATGAATCACAACTGGGTGAAAGAAACCTACACGGCCATCTGGAAAGGTGAGCGTGAGGCCGATGCGGCGAAATCTG
AGTTGGTTGAAGCCAATCTTCGTTTGGTTGTATCCATCGCGAAGAAGTACACCAACCGCGGTCTGCAATTCCTGGATCTG
ATCCAGGAAGGTAACATCGGTTTGATGAAAGCCGTTGATAAGTTCGAATACCGTCGTGGTTACAAATTCTCGACTTATGC
GACTTGGTGGATCCGTCAGGCGATCACTCGTGCTATTGCCGATCAGGCGCGCACGATCCGTATCCCTGTACATATGATCG
AAACCATCAACAAACTTGTTCGTACATCCCGTTACTTGATCCAGGAACTTGGCCGTGAGCCGACCCCGGAAGAGATCGCG
GATAAAATGGACATGCCAGTTGATAAGGTTCGTAAAGTTCTGAAAATTGCGAAAGAACCTATCTCTCTGGAAACTCCAGT
GGGTGAAGAGGAAGATTCGCACCTGGGCGACTTTATCGAGGACAAAAAGGTTATCAATCCTGCAGAAGCGATTGTGAACC
TGAATCTTGCTGAACAAACCCGCCGTGTGCTGTCGACCTTGACTCCAAGAGAAGAAAAAGTCCTGCGCATGCGTTTCGGT
ATCGGGGAAGAGTCAGATCATACTTTGGAAGAAGTGGGTCAGGACTTCAACGTCACCCGTGAGCGTATCCGTCAGATCGA
GGCGAAAGCTCTAAGAAAGCTTCGTCATCCTTCTCGCAGCAACAAACTGAAAGCGTTTGTTGATTCCTAG

Upstream 100 bases:

>100_bases
GTGATCTTAAAAACGAACCTAGTTCGGATAAGTTGGAACAGATTATGAATATTCAGAGAAACCGAATCTCTCTGAATAAA
GGGTAAAAGGCGGGGTATTG

Downstream 100 bases:

>100_bases
TGGCCAGCGGCCGCAGTGCCGTTAGGCTGAAGCAGCTCAAATTTCAAAGGCTCCCTTCGGGGAGCCTTTTTTCGTTTAGG
CTGATGCACTGTGGTTTTGC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MKPNLPNKQEKEPVKVLSLQEQETLIKEEIQRFLKLAKEKGALTIEEINELLPPEIVAPSVLDSFMQGLEAGGVVISDLS
ESSKEEEGEGAFLENVDSEDEEIDDEEKAESEDVKGNDPVRLYLRKMGSVSLLTREGEVEIARRIEKGEREIVRAILLSP
LGTYEIIQLGKRLDEGRIKVKSIFRGLEDEDTQYDEKEYIDKIHELIGRVTEYQKAAEKLFMTLRKEETNTPARQAAMKE
LVAMNDKLMANFESVNFNRKTINRVVIKFKNLVNRIGTLRRRIKDGVERTFSKDVTAMAERMKLIDANEKELTRMTRDTG
LNYQKFKSYVLQAQEAEKRISRLDSETEMNHNWVKETYTAIWKGEREADAAKSELVEANLRLVVSIAKKYTNRGLQFLDL
IQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRTSRYLIQELGREPTPEEIA
DKMDMPVDKVRKVLKIAKEPISLETPVGEEEDSHLGDFIEDKKVINPAEAIVNLNLAEQTRRVLSTLTPREEKVLRMRFG
IGEESDHTLEEVGQDFNVTRERIRQIEAKALRKLRHPSRSNKLKAFVDS

Sequences:

>Translated_609_residues
MKPNLPNKQEKEPVKVLSLQEQETLIKEEIQRFLKLAKEKGALTIEEINELLPPEIVAPSVLDSFMQGLEAGGVVISDLS
ESSKEEEGEGAFLENVDSEDEEIDDEEKAESEDVKGNDPVRLYLRKMGSVSLLTREGEVEIARRIEKGEREIVRAILLSP
LGTYEIIQLGKRLDEGRIKVKSIFRGLEDEDTQYDEKEYIDKIHELIGRVTEYQKAAEKLFMTLRKEETNTPARQAAMKE
LVAMNDKLMANFESVNFNRKTINRVVIKFKNLVNRIGTLRRRIKDGVERTFSKDVTAMAERMKLIDANEKELTRMTRDTG
LNYQKFKSYVLQAQEAEKRISRLDSETEMNHNWVKETYTAIWKGEREADAAKSELVEANLRLVVSIAKKYTNRGLQFLDL
IQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRTSRYLIQELGREPTPEEIA
DKMDMPVDKVRKVLKIAKEPISLETPVGEEEDSHLGDFIEDKKVINPAEAIVNLNLAEQTRRVLSTLTPREEKVLRMRFG
IGEESDHTLEEVGQDFNVTRERIRQIEAKALRKLRHPSRSNKLKAFVDS
>Mature_609_residues
MKPNLPNKQEKEPVKVLSLQEQETLIKEEIQRFLKLAKEKGALTIEEINELLPPEIVAPSVLDSFMQGLEAGGVVISDLS
ESSKEEEGEGAFLENVDSEDEEIDDEEKAESEDVKGNDPVRLYLRKMGSVSLLTREGEVEIARRIEKGEREIVRAILLSP
LGTYEIIQLGKRLDEGRIKVKSIFRGLEDEDTQYDEKEYIDKIHELIGRVTEYQKAAEKLFMTLRKEETNTPARQAAMKE
LVAMNDKLMANFESVNFNRKTINRVVIKFKNLVNRIGTLRRRIKDGVERTFSKDVTAMAERMKLIDANEKELTRMTRDTG
LNYQKFKSYVLQAQEAEKRISRLDSETEMNHNWVKETYTAIWKGEREADAAKSELVEANLRLVVSIAKKYTNRGLQFLDL
IQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRTSRYLIQELGREPTPEEIA
DKMDMPVDKVRKVLKIAKEPISLETPVGEEEDSHLGDFIEDKKVINPAEAIVNLNLAEQTRRVLSTLTPREEKVLRMRFG
IGEESDHTLEEVGQDFNVTRERIRQIEAKALRKLRHPSRSNKLKAFVDS

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=609, Percent_Identity=43.1855500821018, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=45.1327433628319, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI1789871, Length=237, Percent_Identity=31.2236286919831, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 70196; Mature: 70196

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNLPNKQEKEPVKVLSLQEQETLIKEEIQRFLKLAKEKGALTIEEINELLPPEIVAPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCHH
VLDSFMQGLEAGGVVISDLSESSKEEEGEGAFLENVDSEDEEIDDEEKAESEDVKGNDPV
HHHHHHHHHHCCCEEEECCHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHCCCCCCCCCHH
RLYLRKMGSVSLLTREGEVEIARRIEKGEREIVRAILLSPLGTYEIIQLGKRLDEGRIKV
HHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
KSIFRGLEDEDTQYDEKEYIDKIHELIGRVTEYQKAAEKLFMTLRKEETNTPARQAAMKE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LVAMNDKLMANFESVNFNRKTINRVVIKFKNLVNRIGTLRRRIKDGVERTFSKDVTAMAE
HHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMKLIDANEKELTRMTRDTGLNYQKFKSYVLQAQEAEKRISRLDSETEMNHNWVKETYTA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHH
IWKGEREADAAKSELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCC
YKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRTSRYLIQELGREPTPEEIA
CCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DKMDMPVDKVRKVLKIAKEPISLETPVGEEEDSHLGDFIEDKKVINPAEAIVNLNLAEQT
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHH
RRVLSTLTPREEKVLRMRFGIGEESDHTLEEVGQDFNVTRERIRQIEAKALRKLRHPSRS
HHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
NKLKAFVDS
CCCHHHCCC
>Mature Secondary Structure
MKPNLPNKQEKEPVKVLSLQEQETLIKEEIQRFLKLAKEKGALTIEEINELLPPEIVAPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCHH
VLDSFMQGLEAGGVVISDLSESSKEEEGEGAFLENVDSEDEEIDDEEKAESEDVKGNDPV
HHHHHHHHHHCCCEEEECCHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHCCCCCCCCCHH
RLYLRKMGSVSLLTREGEVEIARRIEKGEREIVRAILLSPLGTYEIIQLGKRLDEGRIKV
HHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
KSIFRGLEDEDTQYDEKEYIDKIHELIGRVTEYQKAAEKLFMTLRKEETNTPARQAAMKE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LVAMNDKLMANFESVNFNRKTINRVVIKFKNLVNRIGTLRRRIKDGVERTFSKDVTAMAE
HHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMKLIDANEKELTRMTRDTGLNYQKFKSYVLQAQEAEKRISRLDSETEMNHNWVKETYTA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHH
IWKGEREADAAKSELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCC
YKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLVRTSRYLIQELGREPTPEEIA
CCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DKMDMPVDKVRKVLKIAKEPISLETPVGEEEDSHLGDFIEDKKVINPAEAIVNLNLAEQT
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHH
RRVLSTLTPREEKVLRMRFGIGEESDHTLEEVGQDFNVTRERIRQIEAKALRKLRHPSRS
HHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
NKLKAFVDS
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]