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The map label for this gene is dnaG [H]

Identifier: 42521866

GI number: 42521866

Start: 226127

End: 227995

Strand: Direct

Name: dnaG [H]

Synonym: Bd0241

Alternate gene names: 42521866

Gene position: 226127-227995 (Clockwise)

Preceding gene: 42521865

Following gene: 42521867

Centisome position: 5.98

GC content: 51.47

Gene sequence:

>1869_bases
ATGCGATTTTCACAAGACTTTATCGAGAAGGTGCAAGAGGCCAACAACCTCGTTGACATCATCTCTCAGTACACTCAGTT
GAAACCTAGTGGTAGCGGCCTTATGGGCCGCTGTCCGTTTCCTGATCACGCTGAAAAAACGGCGTCTTTCTCCGTTTCTG
AAACCAAACAGGTCTATCACTGTTTCGGTTGTCACAAGAGTGGCAACCTGTTTTCGTTTTTGCGCGACTATCAGGGTATG
AGTTTCCCTGAGGCGGTGGAATATCTTGCCGGAAGAGCCAGCATCCCGATGCCAGCTCCGGACAAGGAAGATGCTCAGCG
CGACCAGGCCAGCGACAAAAAGAAGTTGTTGTTAAAAGTAAACAAGCTCGCTGCTGATTATTTTTCAGAGCAACTTAAGC
GTGCTCCTCACGATCATCCGGTGAAGAAGTATATCGCCAGCCGGGGGCTGTCCCAGGAAGTGATCGAAACTTTCGGCGTG
GGTTATGCCCTGCCGGAATGGGACGGGCTGGAGCAATACCTGCTAAGCAAAAATGTGCCGATGGCGCTGGCCGAAGAAGC
CCGCCTGGTGAAGGCCCGCAACAACAAGTCCGGACACTTTGATATTTTCCGTGACCGCTTGATGTTCCCGATTCTTTCCG
CGATGGGTGAACCCATTGCCTTCGGGGGCCGTTATTTAGAGAAAAAAGAAAACGAACCCAAATACCTGAACTCTCCAGAG
ACCCCGGTCTTTGTGAAAGGGAAGGTGTTGTACGGACTGTCGCAGACAGCCCGCTACATCCGCAGCGAGGATCTGGCCCT
TATAGTAGAAGGGTATATGGATCTGGTGTCCCTGTATCAAGCCGGCATTCGCAATGTCGTGGCGACGATGGGAACCGCGC
TGACGCCGGACCACGGGAAAATGCTGAAGCGCATGACCAAGAACGTGGTGGCCCTGTTTGACGGGGACAATGCCGGGATG
GAAGCGGCCGAGCGCAGTTTGCCGATCCTGCTGGCGGCGGATCTTTATCCAAAGGGGCTGACGCTGCCGAATGGAATGGA
TCCCGATGATTATGTGAAGAAATACGGATCTGAGGCCCTGAAAGTCGAGCTGGAACGCGCTCCGGACCTGTTTGTGATGA
TTTTGGCCCGTTGGATGGAAGGTTATCGCGGTGACGCCTCTGAAAAGGTGAAGCTCGCGGATAAGTTGAAGCCTCTGTTT
GAGTCCATCCCGGATCTGCGTTTAAGGGACCTGTATTTGGCCGAGGCGGCCCAGAAAATGAACGTAGGCCTTCCTTGGTT
GCGTCAGGCTGTGGGAATACAGGGCAGTGGTCCTGTAAATTCACAAAATCGCCCCGCAATGACTAGGACGGTGCAGCCAA
ACCCGAACATGGGCTCGGCTAACCAGAAACCCCCGCAAGAAGTGTCCTCTAGTGTAGAGGGCGGCAAAATCAGTCTGCAG
GGGGCCTCTAAAGCGGAGCTGATGCTGTTGGGATTGGTACTCAAAAGTCGTGCCAATTTCGATCAATTTGTGAATGAAAA
TCTGCTCGAGAGTGTGGCCCACGAAGGTGTGAAAAAGATCCTCGAAAAAGCCACGGATGTGTATAGACAAGATCTCAGTA
AGTTTGATAAATTGACCAGTCTGCTTGTCTCTTACGTCGATCAGCCTGAATATTTATTCCAAGTGAGCCCAGCGAATGAG
GGCGATCCAGGGTACGACGAAGAGGCAGAAACGAAACTCCTGCGCGATTGCTTTAAGCGCGTAAAAGAAAATTTCCTGCG
CGATCAAGCAAAGCGCTTAGCACGTGATCTTAAAAACGAACCTAGTTCGGATAAGTTGGAACAGATTATGAATATTCAGA
GAAACCGAATCTCTCTGAATAAAGGGTAA

Upstream 100 bases:

>100_bases
CTGTGATGAAAGAAGTCATCGCGAAATCCGGCGGCGCCGCAGACAACAAAGTAGTGAGCGAAGTTATTAAATCCAAGCTT
TCCTAATTTGTTGGAGATAC

Downstream 100 bases:

>100_bases
AAGGCGGGGTATTGATGAAACCGAACCTACCAAATAAACAAGAGAAAGAGCCTGTGAAGGTTCTTTCTTTGCAGGAACAG
GAAACTCTGATTAAAGAAGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MRFSQDFIEKVQEANNLVDIISQYTQLKPSGSGLMGRCPFPDHAEKTASFSVSETKQVYHCFGCHKSGNLFSFLRDYQGM
SFPEAVEYLAGRASIPMPAPDKEDAQRDQASDKKKLLLKVNKLAADYFSEQLKRAPHDHPVKKYIASRGLSQEVIETFGV
GYALPEWDGLEQYLLSKNVPMALAEEARLVKARNNKSGHFDIFRDRLMFPILSAMGEPIAFGGRYLEKKENEPKYLNSPE
TPVFVKGKVLYGLSQTARYIRSEDLALIVEGYMDLVSLYQAGIRNVVATMGTALTPDHGKMLKRMTKNVVALFDGDNAGM
EAAERSLPILLAADLYPKGLTLPNGMDPDDYVKKYGSEALKVELERAPDLFVMILARWMEGYRGDASEKVKLADKLKPLF
ESIPDLRLRDLYLAEAAQKMNVGLPWLRQAVGIQGSGPVNSQNRPAMTRTVQPNPNMGSANQKPPQEVSSSVEGGKISLQ
GASKAELMLLGLVLKSRANFDQFVNENLLESVAHEGVKKILEKATDVYRQDLSKFDKLTSLLVSYVDQPEYLFQVSPANE
GDPGYDEEAETKLLRDCFKRVKENFLRDQAKRLARDLKNEPSSDKLEQIMNIQRNRISLNKG

Sequences:

>Translated_622_residues
MRFSQDFIEKVQEANNLVDIISQYTQLKPSGSGLMGRCPFPDHAEKTASFSVSETKQVYHCFGCHKSGNLFSFLRDYQGM
SFPEAVEYLAGRASIPMPAPDKEDAQRDQASDKKKLLLKVNKLAADYFSEQLKRAPHDHPVKKYIASRGLSQEVIETFGV
GYALPEWDGLEQYLLSKNVPMALAEEARLVKARNNKSGHFDIFRDRLMFPILSAMGEPIAFGGRYLEKKENEPKYLNSPE
TPVFVKGKVLYGLSQTARYIRSEDLALIVEGYMDLVSLYQAGIRNVVATMGTALTPDHGKMLKRMTKNVVALFDGDNAGM
EAAERSLPILLAADLYPKGLTLPNGMDPDDYVKKYGSEALKVELERAPDLFVMILARWMEGYRGDASEKVKLADKLKPLF
ESIPDLRLRDLYLAEAAQKMNVGLPWLRQAVGIQGSGPVNSQNRPAMTRTVQPNPNMGSANQKPPQEVSSSVEGGKISLQ
GASKAELMLLGLVLKSRANFDQFVNENLLESVAHEGVKKILEKATDVYRQDLSKFDKLTSLLVSYVDQPEYLFQVSPANE
GDPGYDEEAETKLLRDCFKRVKENFLRDQAKRLARDLKNEPSSDKLEQIMNIQRNRISLNKG
>Mature_622_residues
MRFSQDFIEKVQEANNLVDIISQYTQLKPSGSGLMGRCPFPDHAEKTASFSVSETKQVYHCFGCHKSGNLFSFLRDYQGM
SFPEAVEYLAGRASIPMPAPDKEDAQRDQASDKKKLLLKVNKLAADYFSEQLKRAPHDHPVKKYIASRGLSQEVIETFGV
GYALPEWDGLEQYLLSKNVPMALAEEARLVKARNNKSGHFDIFRDRLMFPILSAMGEPIAFGGRYLEKKENEPKYLNSPE
TPVFVKGKVLYGLSQTARYIRSEDLALIVEGYMDLVSLYQAGIRNVVATMGTALTPDHGKMLKRMTKNVVALFDGDNAGM
EAAERSLPILLAADLYPKGLTLPNGMDPDDYVKKYGSEALKVELERAPDLFVMILARWMEGYRGDASEKVKLADKLKPLF
ESIPDLRLRDLYLAEAAQKMNVGLPWLRQAVGIQGSGPVNSQNRPAMTRTVQPNPNMGSANQKPPQEVSSSVEGGKISLQ
GASKAELMLLGLVLKSRANFDQFVNENLLESVAHEGVKKILEKATDVYRQDLSKFDKLTSLLVSYVDQPEYLFQVSPANE
GDPGYDEEAETKLLRDCFKRVKENFLRDQAKRLARDLKNEPSSDKLEQIMNIQRNRISLNKG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=426, Percent_Identity=35.6807511737089, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69684; Mature: 69684

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSQDFIEKVQEANNLVDIISQYTQLKPSGSGLMGRCPFPDHAEKTASFSVSETKQVYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHH
CFGCHKSGNLFSFLRDYQGMSFPEAVEYLAGRASIPMPAPDKEDAQRDQASDKKKLLLKV
HHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NKLAADYFSEQLKRAPHDHPVKKYIASRGLSQEVIETFGVGYALPEWDGLEQYLLSKNVP
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
MALAEEARLVKARNNKSGHFDIFRDRLMFPILSAMGEPIAFGGRYLEKKENEPKYLNSPE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCCCCCCC
TPVFVKGKVLYGLSQTARYIRSEDLALIVEGYMDLVSLYQAGIRNVVATMGTALTPDHGK
CCEEEECCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MLKRMTKNVVALFDGDNAGMEAAERSLPILLAADLYPKGLTLPNGMDPDDYVKKYGSEAL
HHHHHHHHEEEEECCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHE
KVELERAPDLFVMILARWMEGYRGDASEKVKLADKLKPLFESIPDLRLRDLYLAEAAQKM
EEEHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NVGLPWLRQAVGIQGSGPVNSQNRPAMTRTVQPNPNMGSANQKPPQEVSSSVEGGKISLQ
CCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEC
GASKAELMLLGLVLKSRANFDQFVNENLLESVAHEGVKKILEKATDVYRQDLSKFDKLTS
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVSYVDQPEYLFQVSPANEGDPGYDEEAETKLLRDCFKRVKENFLRDQAKRLARDLKNE
HHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSSDKLEQIMNIQRNRISLNKG
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRFSQDFIEKVQEANNLVDIISQYTQLKPSGSGLMGRCPFPDHAEKTASFSVSETKQVYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHH
CFGCHKSGNLFSFLRDYQGMSFPEAVEYLAGRASIPMPAPDKEDAQRDQASDKKKLLLKV
HHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NKLAADYFSEQLKRAPHDHPVKKYIASRGLSQEVIETFGVGYALPEWDGLEQYLLSKNVP
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
MALAEEARLVKARNNKSGHFDIFRDRLMFPILSAMGEPIAFGGRYLEKKENEPKYLNSPE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCCCCCCC
TPVFVKGKVLYGLSQTARYIRSEDLALIVEGYMDLVSLYQAGIRNVVATMGTALTPDHGK
CCEEEECCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MLKRMTKNVVALFDGDNAGMEAAERSLPILLAADLYPKGLTLPNGMDPDDYVKKYGSEAL
HHHHHHHHEEEEECCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHE
KVELERAPDLFVMILARWMEGYRGDASEKVKLADKLKPLFESIPDLRLRDLYLAEAAQKM
EEEHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NVGLPWLRQAVGIQGSGPVNSQNRPAMTRTVQPNPNMGSANQKPPQEVSSSVEGGKISLQ
CCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEC
GASKAELMLLGLVLKSRANFDQFVNENLLESVAHEGVKKILEKATDVYRQDLSKFDKLTS
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVSYVDQPEYLFQVSPANEGDPGYDEEAETKLLRDCFKRVKENFLRDQAKRLARDLKNE
HHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSSDKLEQIMNIQRNRISLNKG
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]