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The map label for this gene is recF [H]

Identifier: 42521653

GI number: 42521653

Start: 2755

End: 3879

Strand: Direct

Name: recF [H]

Synonym: Bd0003

Alternate gene names: 42521653

Gene position: 2755-3879 (Clockwise)

Preceding gene: 42521652

Following gene: 42521654

Centisome position: 0.07

GC content: 47.02

Gene sequence:

>1125_bases
ATGATTTTCGAAAGACTGCGTCTGGTTAATTTCCGAAATTATCGGGACGTGGTTTTGTCCTTCTCTCCTCGGGTGAATGT
TTTCCTCGGAGAGAACGGACAGGGTAAAACAAATCTCCTTGAGGCGATGTACATGATCTCTCAAGGGGACTCCTTCCGCT
ACTCTGACAACTCCACCCTGATCAACACGAACACTTCCGAGTCGGTTATCCAGGCGCTGATTACTCAGAACGATCTTCAT
TACAAACTGAAACTGGGTCTTTCAAAAAGCCGCAAGGTTTTAACCCTGAACGACAAGCGTGTGAACTCGGCCGACATCCG
AAAGATTTTTGCCAGTGTCGTGTTCAGTCCTGAAAGTCTATCTTCGATCAAAGAAGGGGCAGATCACCGCCGCGAGCTGG
TGGATGAGTTGCTCGTAACTTTTGACCGAAAAAATGCCCAGTTGATCGCCGATTACCGAAAAGCGCTGAAAACGCGCAAT
AAGATCCTGAAGAACTTTTTGGAGGGTTTGCAGGATAAAGTGGTCACCCAGAACCTTTTGGAGTCTCTAAACCCCCAATT
TGTGCGTTTAGCGACCGATCTGACCCACGCCCGCATCACGGCATTGCACGGACTTTCCAAAGATTTCAATAATGCCATGC
AGTACATTTCTGGCAATTCCTCTGTGGATATCTCGGTGGAATACCTTGTTTCCGATCAAAATGCGGTGAGTTTCACACGT
GAAGAAGTTGAAAATGCAATCACAAAACGACTTCGCGAACTGCATGATGCGGAGCTCTCAAGTGGAACAAGTTTGGTGGG
TCCTCATAAGCACGACATTGTGTTCCTATATGGGCAAAAAGACTCACGATTTTTCTGTAGCCAGGGGCAGCAGAGAGCGA
TCATTCTTTCTTTCAAAATGGCCCAAATTGTGTATCATAGGAAGGCTCACGGGACCTATCCTGTGCTGATGTTGGACGAT
GTACTTTCTGAGCTCGATAAAGCCAAAAGAGATGCACTGATTACGTTCTTACACGAGATCAATACGCAGATTTTTGTGAC
GACGACGGATTTCACATTGCCTGAATCATTCAGCCTCGATCAGCTCCGCGTGGTGCGCATCAAGGATGGCCAAATCCTTG
AGTAG

Upstream 100 bases:

>100_bases
ACAAAATTGATTTCGAACTGAATGATCATCTTTCACCAGGATTGATGAGACCTCACAACGACCAGACTTACACCTGCGTT
GTGATGCCAATGAGAATCTA

Downstream 100 bases:

>100_bases
CTTTCTGCAGTTTCTCAACTGCGGAAAATGATGAACGCGACACCTTCGAATCTGTCGAGGGGTATGCAAAGCAATGAGGG
GTTCCAGTGTCAGCAGAAGA

Product: DNA repair and genetic recombination protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIFERLRLVNFRNYRDVVLSFSPRVNVFLGENGQGKTNLLEAMYMISQGDSFRYSDNSTLINTNTSESVIQALITQNDLH
YKLKLGLSKSRKVLTLNDKRVNSADIRKIFASVVFSPESLSSIKEGADHRRELVDELLVTFDRKNAQLIADYRKALKTRN
KILKNFLEGLQDKVVTQNLLESLNPQFVRLATDLTHARITALHGLSKDFNNAMQYISGNSSVDISVEYLVSDQNAVSFTR
EEVENAITKRLRELHDAELSSGTSLVGPHKHDIVFLYGQKDSRFFCSQGQQRAIILSFKMAQIVYHRKAHGTYPVLMLDD
VLSELDKAKRDALITFLHEINTQIFVTTTDFTLPESFSLDQLRVVRIKDGQILE

Sequences:

>Translated_374_residues
MIFERLRLVNFRNYRDVVLSFSPRVNVFLGENGQGKTNLLEAMYMISQGDSFRYSDNSTLINTNTSESVIQALITQNDLH
YKLKLGLSKSRKVLTLNDKRVNSADIRKIFASVVFSPESLSSIKEGADHRRELVDELLVTFDRKNAQLIADYRKALKTRN
KILKNFLEGLQDKVVTQNLLESLNPQFVRLATDLTHARITALHGLSKDFNNAMQYISGNSSVDISVEYLVSDQNAVSFTR
EEVENAITKRLRELHDAELSSGTSLVGPHKHDIVFLYGQKDSRFFCSQGQQRAIILSFKMAQIVYHRKAHGTYPVLMLDD
VLSELDKAKRDALITFLHEINTQIFVTTTDFTLPESFSLDQLRVVRIKDGQILE
>Mature_374_residues
MIFERLRLVNFRNYRDVVLSFSPRVNVFLGENGQGKTNLLEAMYMISQGDSFRYSDNSTLINTNTSESVIQALITQNDLH
YKLKLGLSKSRKVLTLNDKRVNSADIRKIFASVVFSPESLSSIKEGADHRRELVDELLVTFDRKNAQLIADYRKALKTRN
KILKNFLEGLQDKVVTQNLLESLNPQFVRLATDLTHARITALHGLSKDFNNAMQYISGNSSVDISVEYLVSDQNAVSFTR
EEVENAITKRLRELHDAELSSGTSLVGPHKHDIVFLYGQKDSRFFCSQGQQRAIILSFKMAQIVYHRKAHGTYPVLMLDD
VLSELDKAKRDALITFLHEINTQIFVTTTDFTLPESFSLDQLRVVRIKDGQILE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=22.4089635854342, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42639; Mature: 42639

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFERLRLVNFRNYRDVVLSFSPRVNVFLGENGQGKTNLLEAMYMISQGDSFRYSDNSTL
CCHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCEE
INTNTSESVIQALITQNDLHYKLKLGLSKSRKVLTLNDKRVNSADIRKIFASVVFSPESL
EECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHH
SSIKEGADHRRELVDELLVTFDRKNAQLIADYRKALKTRNKILKNFLEGLQDKVVTQNLL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLNPQFVRLATDLTHARITALHGLSKDFNNAMQYISGNSSVDISVEYLVSDQNAVSFTR
HHCCCCEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHH
EEVENAITKRLRELHDAELSSGTSLVGPHKHDIVFLYGQKDSRFFCSQGQQRAIILSFKM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCEEEEEEHHH
AQIVYHRKAHGTYPVLMLDDVLSELDKAKRDALITFLHEINTQIFVTTTDFTLPESFSLD
HHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
QLRVVRIKDGQILE
CEEEEEECCCCCCC
>Mature Secondary Structure
MIFERLRLVNFRNYRDVVLSFSPRVNVFLGENGQGKTNLLEAMYMISQGDSFRYSDNSTL
CCHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCEE
INTNTSESVIQALITQNDLHYKLKLGLSKSRKVLTLNDKRVNSADIRKIFASVVFSPESL
EECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHH
SSIKEGADHRRELVDELLVTFDRKNAQLIADYRKALKTRNKILKNFLEGLQDKVVTQNLL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLNPQFVRLATDLTHARITALHGLSKDFNNAMQYISGNSSVDISVEYLVSDQNAVSFTR
HHCCCCEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHH
EEVENAITKRLRELHDAELSSGTSLVGPHKHDIVFLYGQKDSRFFCSQGQQRAIILSFKM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCEEEEEEHHH
AQIVYHRKAHGTYPVLMLDDVLSELDKAKRDALITFLHEINTQIFVTTTDFTLPESFSLD
HHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
QLRVVRIKDGQILE
CEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA