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The map label for this gene is gyrB [H]

Identifier: 42521654

GI number: 42521654

Start: 3966

End: 6386

Strand: Direct

Name: gyrB [H]

Synonym: Bd0004

Alternate gene names: 42521654

Gene position: 3966-6386 (Clockwise)

Preceding gene: 42521653

Following gene: 42521655

Centisome position: 0.1

GC content: 48.04

Gene sequence:

>2421_bases
GTGTCAGCAGAAGAACAAAAATCCTATTCAGCCGATTCAATTCAGGTTCTCGAAGGTCTCGAGGCGGTTCGTAAGCGTCC
CGGTATGTATATCGGTGATACCGCTTTCAAGGGCTACCATCACCTTGTGTATGAAATTGTGGATAACTCGGTAGACGAAC
ATTTGGCTGGTTACTGCAAAAAAATCAGTGTCTCCATCAACGCCGATGAATCCATCACAGTCGAAGATGACGGTCGTGGT
ATTCCGGTGGCTACTCAGAAACAAACCGGCAAGTCCGCGCTAGAGCTGGTGATGACCGTTCTCCACGCCGGTGGTAAATT
TGACGGCGGCGGATACAAAGTGTCCGGTGGTCTGCACGGTGTGGGTGCTTCCGTTGTGAATGCGCTTTCTGACCGTGTGT
CCGTGGAAGTTCAGCGTGAAGGTCATTTCTGGAGACAGAACTACGAGCGCGGAAAAATCCTGGCGCCCGTGGCTCAGGGT
GAAACCACCACAAAAACTGGAACGAAAGTAACTTTCAAACCGGATCGCACCATCTTCAAAGACGAAACTCTGAGCTACGA
CTTCACTACTCTGGCGAATCGTTTCCGTGAATTGGCATTCCTGAATGCGGGCCTGCATATCTCTTTGACCGATGAACGCA
ACGGCAAAAAGCAGGACTTCCAGTATTCCGAGGGTGTGGCTGAATTCGTGAAGTACATGAATCAGTCCAAAAAATCCCTG
CACAACGAAGTGGTTTACTTCAAAGGCGAAAAAGACAATGTCGAAGTCGAAATCGCCATGCAGTGGAATGATTCCTACTC
TGAATCTGTTTTCACTTATTGCAACAACATCAACACGCATGAAGGTGGTACTCACCTTGTCGGCTTCCGTGCCGCTTTGA
CTCGTACAACGAATGCGTATGCGACTGAAAAAAATCTTCTGAAAGATCTGAAAGCCAACCTTGAGGGGGAAGACATCCGT
GAAGGTCTGGCAGCCGTTATCTCTGTGAAAGTTCGTGAACCACAGTTCGAAGGTCAGACCAAAACCAAGCTGGGTAATGC
CGAAGTCAAAGGTATCGTCGAAACCCTGGTGAATGAAAAACTGGCCGACTGGATGGATCGCAATCCATCTGTAGCAAAAA
ACATCATCATGAAATGTGTGGAATCCGCGCGTGCCCGTGAAGCTGCCAGAAAAGCCCGTGATCTGACTCGTCGTAAGACA
GTTCTGGATGGTGGCTCTCTGCCAGGTAAAATGGCGGACTGTCAGGAACGGGACCCGGCACTTTGTGAACTGTACCTGGT
GGAGGGGGACTCCGCAGGTGGATCCGCGAAGCAAGGACGCGACCGTCGCACGCAGGCGATTTTGCCGTTGAAAGGTAAAA
TCCTGAACGTCGAAAAAGCGCGTTTTGACAAAATCATTTCTTCGGAAGAGATCAAAGTCATTATCTCCGCTTTGGGGACC
GGTATCGGTAAAGACAACGTGAATGTGGATAAAATCCGCTATCACAAAATCATCATCATGACCGATGCCGACGTCGATGG
ATCCCACATCATGACTTTGCTTTTGACGTTCTTCTACCGTCAGATGCCACTGGTGCTTGAGCGTGGTTATGTGTATATCG
CGCAACCACCTTTGTACCGTGCGAAAAAAGGCAAAGAGGAAACTTACCTGAAAAATGAAGCGGCCTTGACTGAGTATCTT
CTGAGCTCGGGCCTGGGTAACTTCAAAATCAAAGGCAAAGAATCCCTGCCGGAAAAAGATCTGCGTCAGCTGATCTTGAA
CATCCAGCGTTTCAACGACCTTCTGCGTGTTTCCTCGAAGAAATACGACAAGGACGTTTTGTACTTCCTGCTAAGCAAAG
TGACTGATTTTGAAAAAACATTCACGGACGCGAAAAAAATCGAACAGGTTCTTACCGACCTGGGTGCGTGGGTTAATTCT
GACCAGACTTTGGGTATCACCGAATACAAAGGTGAAGTTAAAACCGAAGCTGAAACCGGAAAAATGTATGCTGACATCTA
CACCGTTCGTTATGCCGACCGAATGACAACAAAATTCAGCACAGACAATCTGCGTTCTTCCGAGATCATCGAGCTTAGAA
AAATCTGGGCCGATATTCAGGCGATCAGCACTTTGCCTTTGACGATTCTTGAGGGTGATAACGAAGTTCAGTTTGAAAAC
TACAATGACTTCTACACTCACGTTATGGAATCCACGAAAAAAGGAATCTACATCCAGCGCTATAAAGGATTGGGAGAGAT
GAATCCGGAGCAGTTGTGGGAAACCACTCTGAACAAGGAAAACAGAACTCTTCTGAAAGTGACTATCGACGATGCCGTTG
CCGCCGACGAAACATTCTCCATCCTGATGGGTGAAATGGTTGAGCCTCGTCGTCAATTCATCCACGACAACGCATTGTTG
GCACGAAGCCTGGACGTTTAA

Upstream 100 bases:

>100_bases
AAATCCTTGAGTAGCTTTCTGCAGTTTCTCAACTGCGGAAAATGATGAACGCGACACCTTCGAATCTGTCGAGGGGTATG
CAAAGCAATGAGGGGTTCCA

Downstream 100 bases:

>100_bases
TTTTTTAAGGGATTTGAAATTTTATGGAAAATAATCAAGAAGAAAAAGGCGTCACGTTAGTTGATATCAGCAAGGAGATG
CGCGGAGCCTATCTGCAGTA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MSAEEQKSYSADSIQVLEGLEAVRKRPGMYIGDTAFKGYHHLVYEIVDNSVDEHLAGYCKKISVSINADESITVEDDGRG
IPVATQKQTGKSALELVMTVLHAGGKFDGGGYKVSGGLHGVGASVVNALSDRVSVEVQREGHFWRQNYERGKILAPVAQG
ETTTKTGTKVTFKPDRTIFKDETLSYDFTTLANRFRELAFLNAGLHISLTDERNGKKQDFQYSEGVAEFVKYMNQSKKSL
HNEVVYFKGEKDNVEVEIAMQWNDSYSESVFTYCNNINTHEGGTHLVGFRAALTRTTNAYATEKNLLKDLKANLEGEDIR
EGLAAVISVKVREPQFEGQTKTKLGNAEVKGIVETLVNEKLADWMDRNPSVAKNIIMKCVESARAREAARKARDLTRRKT
VLDGGSLPGKMADCQERDPALCELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKIISSEEIKVIISALGT
GIGKDNVNVDKIRYHKIIIMTDADVDGSHIMTLLLTFFYRQMPLVLERGYVYIAQPPLYRAKKGKEETYLKNEAALTEYL
LSSGLGNFKIKGKESLPEKDLRQLILNIQRFNDLLRVSSKKYDKDVLYFLLSKVTDFEKTFTDAKKIEQVLTDLGAWVNS
DQTLGITEYKGEVKTEAETGKMYADIYTVRYADRMTTKFSTDNLRSSEIIELRKIWADIQAISTLPLTILEGDNEVQFEN
YNDFYTHVMESTKKGIYIQRYKGLGEMNPEQLWETTLNKENRTLLKVTIDDAVAADETFSILMGEMVEPRRQFIHDNALL
ARSLDV

Sequences:

>Translated_806_residues
MSAEEQKSYSADSIQVLEGLEAVRKRPGMYIGDTAFKGYHHLVYEIVDNSVDEHLAGYCKKISVSINADESITVEDDGRG
IPVATQKQTGKSALELVMTVLHAGGKFDGGGYKVSGGLHGVGASVVNALSDRVSVEVQREGHFWRQNYERGKILAPVAQG
ETTTKTGTKVTFKPDRTIFKDETLSYDFTTLANRFRELAFLNAGLHISLTDERNGKKQDFQYSEGVAEFVKYMNQSKKSL
HNEVVYFKGEKDNVEVEIAMQWNDSYSESVFTYCNNINTHEGGTHLVGFRAALTRTTNAYATEKNLLKDLKANLEGEDIR
EGLAAVISVKVREPQFEGQTKTKLGNAEVKGIVETLVNEKLADWMDRNPSVAKNIIMKCVESARAREAARKARDLTRRKT
VLDGGSLPGKMADCQERDPALCELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKIISSEEIKVIISALGT
GIGKDNVNVDKIRYHKIIIMTDADVDGSHIMTLLLTFFYRQMPLVLERGYVYIAQPPLYRAKKGKEETYLKNEAALTEYL
LSSGLGNFKIKGKESLPEKDLRQLILNIQRFNDLLRVSSKKYDKDVLYFLLSKVTDFEKTFTDAKKIEQVLTDLGAWVNS
DQTLGITEYKGEVKTEAETGKMYADIYTVRYADRMTTKFSTDNLRSSEIIELRKIWADIQAISTLPLTILEGDNEVQFEN
YNDFYTHVMESTKKGIYIQRYKGLGEMNPEQLWETTLNKENRTLLKVTIDDAVAADETFSILMGEMVEPRRQFIHDNALL
ARSLDV
>Mature_805_residues
SAEEQKSYSADSIQVLEGLEAVRKRPGMYIGDTAFKGYHHLVYEIVDNSVDEHLAGYCKKISVSINADESITVEDDGRGI
PVATQKQTGKSALELVMTVLHAGGKFDGGGYKVSGGLHGVGASVVNALSDRVSVEVQREGHFWRQNYERGKILAPVAQGE
TTTKTGTKVTFKPDRTIFKDETLSYDFTTLANRFRELAFLNAGLHISLTDERNGKKQDFQYSEGVAEFVKYMNQSKKSLH
NEVVYFKGEKDNVEVEIAMQWNDSYSESVFTYCNNINTHEGGTHLVGFRAALTRTTNAYATEKNLLKDLKANLEGEDIRE
GLAAVISVKVREPQFEGQTKTKLGNAEVKGIVETLVNEKLADWMDRNPSVAKNIIMKCVESARAREAARKARDLTRRKTV
LDGGSLPGKMADCQERDPALCELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKIISSEEIKVIISALGTG
IGKDNVNVDKIRYHKIIIMTDADVDGSHIMTLLLTFFYRQMPLVLERGYVYIAQPPLYRAKKGKEETYLKNEAALTEYLL
SSGLGNFKIKGKESLPEKDLRQLILNIQRFNDLLRVSSKKYDKDVLYFLLSKVTDFEKTFTDAKKIEQVLTDLGAWVNSD
QTLGITEYKGEVKTEAETGKMYADIYTVRYADRMTTKFSTDNLRSSEIIELRKIWADIQAISTLPLTILEGDNEVQFENY
NDFYTHVMESTKKGIYIQRYKGLGEMNPEQLWETTLNKENRTLLKVTIDDAVAADETFSILMGEMVEPRRQFIHDNALLA
RSLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=580, Percent_Identity=25, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI19913408, Length=577, Percent_Identity=25.8232235701906, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI48994957, Length=809, Percent_Identity=50.6798516687268, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=592, Percent_Identity=39.8648648648649, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI212645845, Length=494, Percent_Identity=27.9352226720648, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17535065, Length=571, Percent_Identity=25.3940455341506, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=159, Percent_Identity=32.7044025157233, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=574, Percent_Identity=26.8292682926829, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17136538, Length=579, Percent_Identity=25.5613126079447, Blast_Score=128, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90639; Mature: 90508

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEEQKSYSADSIQVLEGLEAVRKRPGMYIGDTAFKGYHHLVYEIVDNSVDEHLAGYCK
CCCCHHHCCCCHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHH
KISVSINADESITVEDDGRGIPVATQKQTGKSALELVMTVLHAGGKFDGGGYKVSGGLHG
EEEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNALSDRVSVEVQREGHFWRQNYERGKILAPVAQGETTTKTGTKVTFKPDRTIFK
HHHHHHHHHHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEEC
DETLSYDFTTLANRFRELAFLNAGLHISLTDERNGKKQDFQYSEGVAEFVKYMNQSKKSL
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HNEVVYFKGEKDNVEVEIAMQWNDSYSESVFTYCNNINTHEGGTHLVGFRAALTRTTNAY
HCCEEEEECCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCHH
ATEKNLLKDLKANLEGEDIREGLAAVISVKVREPQFEGQTKTKLGNAEVKGIVETLVNEK
HHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHH
LADWMDRNPSVAKNIIMKCVESARAREAARKARDLTRRKTVLDGGSLPGKMADCQERDPA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LCELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKIISSEEIKVIISALGT
EEEEEEEECCCCCCCCCCCCCCHHEEECCCCCCEEECHHHHHHHHCCCHHHHHHHHHHCC
GIGKDNVNVDKIRYHKIIIMTDADVDGSHIMTLLLTFFYRQMPLVLERGYVYIAQPPLYR
CCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCHH
AKKGKEETYLKNEAALTEYLLSSGLGNFKIKGKESLPEKDLRQLILNIQRFNDLLRVSSK
HCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KYDKDVLYFLLSKVTDFEKTFTDAKKIEQVLTDLGAWVNSDQTLGITEYKGEVKTEAETG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
KMYADIYTVRYADRMTTKFSTDNLRSSEIIELRKIWADIQAISTLPLTILEGDNEVQFEN
CEEEEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECC
YNDFYTHVMESTKKGIYIQRYKGLGEMNPEQLWETTLNKENRTLLKVTIDDAVAADETFS
CHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCHHCCCHHHH
ILMGEMVEPRRQFIHDNALLARSLDV
HHHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
SAEEQKSYSADSIQVLEGLEAVRKRPGMYIGDTAFKGYHHLVYEIVDNSVDEHLAGYCK
CCCHHHCCCCHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHH
KISVSINADESITVEDDGRGIPVATQKQTGKSALELVMTVLHAGGKFDGGGYKVSGGLHG
EEEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNALSDRVSVEVQREGHFWRQNYERGKILAPVAQGETTTKTGTKVTFKPDRTIFK
HHHHHHHHHHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEEC
DETLSYDFTTLANRFRELAFLNAGLHISLTDERNGKKQDFQYSEGVAEFVKYMNQSKKSL
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HNEVVYFKGEKDNVEVEIAMQWNDSYSESVFTYCNNINTHEGGTHLVGFRAALTRTTNAY
HCCEEEEECCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCHH
ATEKNLLKDLKANLEGEDIREGLAAVISVKVREPQFEGQTKTKLGNAEVKGIVETLVNEK
HHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHH
LADWMDRNPSVAKNIIMKCVESARAREAARKARDLTRRKTVLDGGSLPGKMADCQERDPA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LCELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKIISSEEIKVIISALGT
EEEEEEEECCCCCCCCCCCCCCHHEEECCCCCCEEECHHHHHHHHCCCHHHHHHHHHHCC
GIGKDNVNVDKIRYHKIIIMTDADVDGSHIMTLLLTFFYRQMPLVLERGYVYIAQPPLYR
CCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCHH
AKKGKEETYLKNEAALTEYLLSSGLGNFKIKGKESLPEKDLRQLILNIQRFNDLLRVSSK
HCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KYDKDVLYFLLSKVTDFEKTFTDAKKIEQVLTDLGAWVNSDQTLGITEYKGEVKTEAETG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
KMYADIYTVRYADRMTTKFSTDNLRSSEIIELRKIWADIQAISTLPLTILEGDNEVQFEN
CEEEEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECC
YNDFYTHVMESTKKGIYIQRYKGLGEMNPEQLWETTLNKENRTLLKVTIDDAVAADETFS
CHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCHHCCCHHHH
ILMGEMVEPRRQFIHDNALLARSLDV
HHHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]