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The map label for this gene is dnaN [H]

Identifier: 42521652

GI number: 42521652

Start: 1649

End: 2755

Strand: Direct

Name: dnaN [H]

Synonym: Bd0002

Alternate gene names: 42521652

Gene position: 1649-2755 (Clockwise)

Preceding gene: 42521651

Following gene: 42521653

Centisome position: 0.04

GC content: 43.18

Gene sequence:

>1107_bases
ATGAAATTAGAGATTGATAAGCGAGATCTGTTAAGTCTTATCGGAAAAACACAGAACATCGTTGAGAAGCGCAACACCAT
GCCCATCCTCATCAATGTTTTGTTGGAAGCTGATCAGAACACTTTGAAAGTTTTTGCCACCGATCTGGAAGTCAGCCTTA
CTGATCAGATCAAAGTTCAAGTTCATCAGGCCGGCAAAGTAGCTGTCAGCGCAAAAAGTCTTTTTGATATCGCCAAGGAA
CTTTCTGAAGGTCCGATCACTTTGATCAAAAAAGAAAACAACTGGTTGGAAATCAAACAAGGGAAATACACATCCAAGAT
TGTGGGTATCTCTGCAGAAGAATATCCGATCTTCCCGACTTACAACTCTCAGGCCTTCATCAAGATTCACGCTCAAGTGC
TGAAAGAGATGATTGATAAAACAATCTACTCTGTGTCCACAGACGAAACTCGCTACCACCTGAACGGTGTGTTTTTTGAA
TTGAATCCTCAGACAGGTTTCAAAATGGTTGCGACCGATGGTCACCGTATGAGCCTGGTGAACAAACCTTCCGAGGACAT
CAAGGTCAATGCCACACAAGGTGTGATCATTCCGCGCAAAGGTCTGCACGAGATTAAAAAAATTCTGGAAAGCATCGACG
GCACTGTGGAAATAGCTGTGGAAGGCTCCCAATTCGTATTGAAACATTCATCAACAATTCTGATGATTCGTTTGATCGAA
GGAAAGTACCCGAATTATCAACAGTTTATTCCACAGAAGCTTCCGCAAAAAGTGATGATCAGCCGTGATGCGTTCCTGAC
TTCACTGAAACGTGTATCCCTTTTGGCGAATCAGAAATCCAAAGCGGTGTTGTTGAATCTATCCAACGGCCGTATGGAGA
TTTCTTCGAACAACCCTGAACTGGGTGATGCAAAAGAAGAGATCGAAGTGGATTACATGGGCGGCGAAATCAAAATCGGT
TTCAATGCGAAGTACATCACTGACATCCTGACCAGCATGAACCAGGACAAAATTGATTTCGAACTGAATGATCATCTTTC
ACCAGGATTGATGAGACCTCACAACGACCAGACTTACACCTGCGTTGTGATGCCAATGAGAATCTAA

Upstream 100 bases:

>100_bases
GACTTAGGGGATTTGTTAGGTTTTCCACGCCCCTACTACTACTACTAGATATTTATATATATAGAATTAGATAATAAGAG
CGTTCAAAAGAGGGTCTTTC

Downstream 100 bases:

>100_bases
TGATTTTCGAAAGACTGCGTCTGGTTAATTTCCGAAATTATCGGGACGTGGTTTTGTCCTTCTCTCCTCGGGTGAATGTT
TTCCTCGGAGAGAACGGACA

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKLEIDKRDLLSLIGKTQNIVEKRNTMPILINVLLEADQNTLKVFATDLEVSLTDQIKVQVHQAGKVAVSAKSLFDIAKE
LSEGPITLIKKENNWLEIKQGKYTSKIVGISAEEYPIFPTYNSQAFIKIHAQVLKEMIDKTIYSVSTDETRYHLNGVFFE
LNPQTGFKMVATDGHRMSLVNKPSEDIKVNATQGVIIPRKGLHEIKKILESIDGTVEIAVEGSQFVLKHSSTILMIRLIE
GKYPNYQQFIPQKLPQKVMISRDAFLTSLKRVSLLANQKSKAVLLNLSNGRMEISSNNPELGDAKEEIEVDYMGGEIKIG
FNAKYITDILTSMNQDKIDFELNDHLSPGLMRPHNDQTYTCVVMPMRI

Sequences:

>Translated_368_residues
MKLEIDKRDLLSLIGKTQNIVEKRNTMPILINVLLEADQNTLKVFATDLEVSLTDQIKVQVHQAGKVAVSAKSLFDIAKE
LSEGPITLIKKENNWLEIKQGKYTSKIVGISAEEYPIFPTYNSQAFIKIHAQVLKEMIDKTIYSVSTDETRYHLNGVFFE
LNPQTGFKMVATDGHRMSLVNKPSEDIKVNATQGVIIPRKGLHEIKKILESIDGTVEIAVEGSQFVLKHSSTILMIRLIE
GKYPNYQQFIPQKLPQKVMISRDAFLTSLKRVSLLANQKSKAVLLNLSNGRMEISSNNPELGDAKEEIEVDYMGGEIKIG
FNAKYITDILTSMNQDKIDFELNDHLSPGLMRPHNDQTYTCVVMPMRI
>Mature_368_residues
MKLEIDKRDLLSLIGKTQNIVEKRNTMPILINVLLEADQNTLKVFATDLEVSLTDQIKVQVHQAGKVAVSAKSLFDIAKE
LSEGPITLIKKENNWLEIKQGKYTSKIVGISAEEYPIFPTYNSQAFIKIHAQVLKEMIDKTIYSVSTDETRYHLNGVFFE
LNPQTGFKMVATDGHRMSLVNKPSEDIKVNATQGVIIPRKGLHEIKKILESIDGTVEIAVEGSQFVLKHSSTILMIRLIE
GKYPNYQQFIPQKLPQKVMISRDAFLTSLKRVSLLANQKSKAVLLNLSNGRMEISSNNPELGDAKEEIEVDYMGGEIKIG
FNAKYITDILTSMNQDKIDFELNDHLSPGLMRPHNDQTYTCVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=370, Percent_Identity=29.1891891891892, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41522; Mature: 41522

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEIDKRDLLSLIGKTQNIVEKRNTMPILINVLLEADQNTLKVFATDLEVSLTDQIKVQ
CEEECCHHHHHHHHCCHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEEEEEECEEEEE
VHQAGKVAVSAKSLFDIAKELSEGPITLIKKENNWLEIKQGKYTSKIVGISAEEYPIFPT
EEECCEEEEEHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCEEEC
YNSQAFIKIHAQVLKEMIDKTIYSVSTDETRYHLNGVFFELNPQTGFKMVATDGHRMSLV
CCCCEEEEEHHHHHHHHHHHHHEECCCCCCEEEEEEEEEEECCCCCEEEEEECCCEEEEE
NKPSEDIKVNATQGVIIPRKGLHEIKKILESIDGTVEIAVEGSQFVLKHSSTILMIRLIE
CCCCCCEEEECCCCEEECCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCEEEEEEEEC
GKYPNYQQFIPQKLPQKVMISRDAFLTSLKRVSLLANQKSKAVLLNLSNGRMEISSNNPE
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCC
LGDAKEEIEVDYMGGEIKIGFNAKYITDILTSMNQDKIDFELNDHLSPGLMRPHNDQTYT
CCCCHHHEEEEEECCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEE
CVVMPMRI
EEEEEECC
>Mature Secondary Structure
MKLEIDKRDLLSLIGKTQNIVEKRNTMPILINVLLEADQNTLKVFATDLEVSLTDQIKVQ
CEEECCHHHHHHHHCCHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEEEEEECEEEEE
VHQAGKVAVSAKSLFDIAKELSEGPITLIKKENNWLEIKQGKYTSKIVGISAEEYPIFPT
EEECCEEEEEHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCEEEC
YNSQAFIKIHAQVLKEMIDKTIYSVSTDETRYHLNGVFFELNPQTGFKMVATDGHRMSLV
CCCCEEEEEHHHHHHHHHHHHHEECCCCCCEEEEEEEEEEECCCCCEEEEEECCCEEEEE
NKPSEDIKVNATQGVIIPRKGLHEIKKILESIDGTVEIAVEGSQFVLKHSSTILMIRLIE
CCCCCCEEEECCCCEEECCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCEEEEEEEEC
GKYPNYQQFIPQKLPQKVMISRDAFLTSLKRVSLLANQKSKAVLLNLSNGRMEISSNNPE
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCC
LGDAKEEIEVDYMGGEIKIGFNAKYITDILTSMNQDKIDFELNDHLSPGLMRPHNDQTYT
CCCCHHHEEEEEECCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEE
CVVMPMRI
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]