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The map label for this gene is mexA [H]

Identifier: 37679579

GI number: 37679579

Start: 1417763

End: 1418803

Strand: Direct

Name: mexA [H]

Synonym: VV1395

Alternate gene names: 37679579

Gene position: 1417763-1418803 (Clockwise)

Preceding gene: 37679578

Following gene: 37679580

Centisome position: 42.26

GC content: 47.65

Gene sequence:

>1041_bases
ATGATAACCATGAGAAAAGCATTGGTTTTTCTTGCTGTCGCCACAGCATTGGGAGGATGTGGTCGAGAGCTTCCTCCTAT
TCCCGAGCCCGATTCGCGGCCTGCCAAGTTATTCACGGTGTCTGTGGGTGACGCTAAATTTGAACGAACTTTTCCTGCTG
TTTCTGAAGCGGGTGATAAGGCAGTTTTAACATTTCGTGTCCCTGGACAATTGCAGACCATTGATGTTACTGCAGGTCAA
AGAGTGACAAAAGGGCAGATTTTAGCCACCTTGAACCCAGATGAGTACGCGTTGCTGGCAAAACAAGCGAGAGCGAATTT
TAAACTGGCGGATGTGCAATATGAGCGCTACAAAAAGCTTCGCGCCGATAAGGTGGTCTCAGAGCAAGACTTCGATCAAG
CACAAGCCAACCACAACTCTGCGCGCGCTACGTTAGAGCAAGCTGAGGCCAACCTTCGTTACACTAAGCTGATTGCACCT
TATGATGGCACCATTTCTCTTATTCCAGCTGAAAATCACGAATACGTTGCGGCCAAACAAGGCGTAATGAATATCCAAAC
CAATCAATTGATGAAGGTGATTTTCCAGTTGCCAGATCATCTATTAGGCCGTTTCTCTCAGGGTGTTGAGCCGAATGCGG
TGATGCGTTTTGATGCATTCCCAGGGAGCGAATTCCCACTGCGCTTCCAAGAAATCGATACCGAAGCGGATACCAAAACC
GGCAGTTATAAAGTCACTATGATCATGGAGCGTCCTGCCGATCTTGGGGTGCTCCCCGGTATGGCTGGTAGTGTCCACGT
GAGTGCCAAATCTCAATCTGCCACACAAATCCCTACCTCTGCCATCTTTAAGCAAGATGAGAAAAAGTATGTTTGGCGAG
TGAATGATCAGAGCATTGTTGAGCTCGCGGAAGTGGAGCTTAACGACAAGCGCGAAGTGGTTAATGGTCTCAATGATGGT
GACCAAATCATTATTTCTGGTGTCAGTGGTATCGAGTCAGGGATAAAAGTTCGTGAATGGGTTAAAGAGCGAGGGCTATA
A

Upstream 100 bases:

>100_bases
AACCTTGCTCTCATTTTATTAAATTTCAGATATTTACAAAGCTGTTAACTGGAACAAAATCATAGTTTCATCTAAGGTAT
AGAGAACATTTTCTAACAGG

Downstream 100 bases:

>100_bases
CGTGAATAAATGGATTGGTTTAACCGCGTTGTCCGCCGCTTTACTGTTAGCCGGATGTGGGCAACAAAATCAGCCAGCCG
CTTTGGCTGACCCAAATGTC

Product: membrane-fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MITMRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVLTFRVPGQLQTIDVTAGQ
RVTKGQILATLNPDEYALLAKQARANFKLADVQYERYKKLRADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAP
YDGTISLIPAENHEYVAAKQGVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKT
GSYKVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELAEVELNDKREVVNGLNDG
DQIIISGVSGIESGIKVREWVKERGL

Sequences:

>Translated_346_residues
MITMRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVLTFRVPGQLQTIDVTAGQ
RVTKGQILATLNPDEYALLAKQARANFKLADVQYERYKKLRADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAP
YDGTISLIPAENHEYVAAKQGVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKT
GSYKVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELAEVELNDKREVVNGLNDG
DQIIISGVSGIESGIKVREWVKERGL
>Mature_346_residues
MITMRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDKAVLTFRVPGQLQTIDVTAGQ
RVTKGQILATLNPDEYALLAKQARANFKLADVQYERYKKLRADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAP
YDGTISLIPAENHEYVAAKQGVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKT
GSYKVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIVELAEVELNDKREVVNGLNDG
DQIIISGVSGIESGIKVREWVKERGL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=306, Percent_Identity=23.8562091503268, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=26.6129032258064, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081796, Length=150, Percent_Identity=34, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38123; Mature: 38123

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCHHHCCCCE
AVLTFRVPGQLQTIDVTAGQRVTKGQILATLNPDEYALLAKQARANFKLADVQYERYKKL
EEEEEECCCCEEEEEECCCCEECCCEEEEEECCCHHHHHHHHHHCCEEEECCHHHHHHHH
RADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDGTISLIPAENHEYVAAKQ
HHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCEEEECC
GVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKT
CCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEHEECCCCCCCCC
GSYKVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIV
CCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEEEEECCHHHH
ELAEVELNDKREVVNGLNDGDQIIISGVSGIESGIKVREWVKERGL
HHHHCCCCCHHHHHCCCCCCCEEEEEECHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MITMRKALVFLAVATALGGCGRELPPIPEPDSRPAKLFTVSVGDAKFERTFPAVSEAGDK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCHHHCCCCE
AVLTFRVPGQLQTIDVTAGQRVTKGQILATLNPDEYALLAKQARANFKLADVQYERYKKL
EEEEEECCCCEEEEEECCCCEECCCEEEEEECCCHHHHHHHHHHCCEEEECCHHHHHHHH
RADKVVSEQDFDQAQANHNSARATLEQAEANLRYTKLIAPYDGTISLIPAENHEYVAAKQ
HHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCEEEECC
GVMNIQTNQLMKVIFQLPDHLLGRFSQGVEPNAVMRFDAFPGSEFPLRFQEIDTEADTKT
CCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEHEECCCCCCCCC
GSYKVTMIMERPADLGVLPGMAGSVHVSAKSQSATQIPTSAIFKQDEKKYVWRVNDQSIV
CCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCEEEEEECCHHHH
ELAEVELNDKREVVNGLNDGDQIIISGVSGIESGIKVREWVKERGL
HHHHCCCCCHHHHHCCCCCCCEEEEEECHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]