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The map label for this gene is menH [H]

Identifier: 37679301

GI number: 37679301

Start: 1125562

End: 1126353

Strand: Direct

Name: menH [H]

Synonym: VV1117

Alternate gene names: 37679301

Gene position: 1125562-1126353 (Clockwise)

Preceding gene: 37679300

Following gene: 161486639

Centisome position: 33.55

GC content: 48.86

Gene sequence:

>792_bases
ATGTCCGTGCTCTCGTTTAATTATCAGCAAGGCAATGGTGATGACCGTTCGCCCGTGTTGGTATTTTTGCACGGATTATT
GGGCAGTGCGGAAGATTGGTGTGCCACGCGATGTTCACTCAAAGCGTATCCAACCCTAACGATTGACCTTGCAGGGCATG
GCCAAAGTGTTGGCATTGCCCCAAGTTGTGCTGCGAAGAGTGCAGAGCAAGTGGCGACGGTGATTGAGCAGCAATTGGCC
CAACGCCCATGTATTCTCATTGGTTATTCAATGGGAGGCCGAATTGTCATGCAGGGGCTTGTCGAGCACGCTTTTGCCGC
ACTGAATCTACGCGGAGCTTTGATTGAAGGCGGCAATTTTGGTTTAGTCAGTGAGCAAGAAAAGCAGCAACGCTGGATCA
GTGATAGCCATTGGGCGAAGCGATTTCGTGAAGAGCCCATTGAACAGGTATTAGCCGATTGGTATCAGCAAGGGGTATTT
TCTTCACTAAACCATGCGCAAAAACAAACCTTGATAGCAAAACGAAGTGCTAATCTTGGTGTGCCAGTCGCGGAGATGCT
ATTAGCCACTTCTTTGGCGAAGCAACCGAATCTTTTGCCTCAGTTGCAACGCTCTAGCGTGCCTTTGCACTATGTATGCG
GCGAAAAAGACACAAAATTTCAAGAACTAGCGAGGCAGAGTGGCTTGTCCTGCTCGATAGTTGACAATGCGGGCCACAAT
GTCCATCAAGAGCAGCCTAACGCTTTTGCTGACATCGTCCGTGCGTTTATTCATTCGGTTTATCAACGATAA

Upstream 100 bases:

>100_bases
CGAATTGTTGGCCATTTTGCACACGGCTGTGGCGCTTTATTGGTTGAAGTCAACACGCCGCCAAACCAAGCGTCACAGCA
CATAAAACAATTGGCTGATC

Downstream 100 bases:

>100_bases
ACACATTAGAACAATGGGAACCACCATGGCTAAAACAGTAGGCATCACTGAAGCAGAACTTTACGCACCAGTTAATTGGA
ATGACTGTAGTGCGCCCTAT

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSVLSFNYQQGNGDDRSPVLVFLHGLLGSAEDWCATRCSLKAYPTLTIDLAGHGQSVGIAPSCAAKSAEQVATVIEQQLA
QRPCILIGYSMGGRIVMQGLVEHAFAALNLRGALIEGGNFGLVSEQEKQQRWISDSHWAKRFREEPIEQVLADWYQQGVF
SSLNHAQKQTLIAKRSANLGVPVAEMLLATSLAKQPNLLPQLQRSSVPLHYVCGEKDTKFQELARQSGLSCSIVDNAGHN
VHQEQPNAFADIVRAFIHSVYQR

Sequences:

>Translated_263_residues
MSVLSFNYQQGNGDDRSPVLVFLHGLLGSAEDWCATRCSLKAYPTLTIDLAGHGQSVGIAPSCAAKSAEQVATVIEQQLA
QRPCILIGYSMGGRIVMQGLVEHAFAALNLRGALIEGGNFGLVSEQEKQQRWISDSHWAKRFREEPIEQVLADWYQQGVF
SSLNHAQKQTLIAKRSANLGVPVAEMLLATSLAKQPNLLPQLQRSSVPLHYVCGEKDTKFQELARQSGLSCSIVDNAGHN
VHQEQPNAFADIVRAFIHSVYQR
>Mature_262_residues
SVLSFNYQQGNGDDRSPVLVFLHGLLGSAEDWCATRCSLKAYPTLTIDLAGHGQSVGIAPSCAAKSAEQVATVIEQQLAQ
RPCILIGYSMGGRIVMQGLVEHAFAALNLRGALIEGGNFGLVSEQEKQQRWISDSHWAKRFREEPIEQVLADWYQQGVFS
SLNHAQKQTLIAKRSANLGVPVAEMLLATSLAKQPNLLPQLQRSSVPLHYVCGEKDTKFQELARQSGLSCSIVDNAGHNV
HQEQPNAFADIVRAFIHSVYQR

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=245, Percent_Identity=39.1836734693878, Blast_Score=173, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 28866; Mature: 28735

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLSFNYQQGNGDDRSPVLVFLHGLLGSAEDWCATRCSLKAYPTLTIDLAGHGQSVGIA
CCEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCEECCEEEEEECCCCCCCCCC
PSCAAKSAEQVATVIEQQLAQRPCILIGYSMGGRIVMQGLVEHAFAALNLRGALIEGGNF
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCCC
GLVSEQEKQQRWISDSHWAKRFREEPIEQVLADWYQQGVFSSLNHAQKQTLIAKRSANLG
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPVAEMLLATSLAKQPNLLPQLQRSSVPLHYVCGEKDTKFQELARQSGLSCSIVDNAGHN
CHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCEEEEECCCCCC
VHQEQPNAFADIVRAFIHSVYQR
CCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVLSFNYQQGNGDDRSPVLVFLHGLLGSAEDWCATRCSLKAYPTLTIDLAGHGQSVGIA
CEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCEECCEEEEEECCCCCCCCCC
PSCAAKSAEQVATVIEQQLAQRPCILIGYSMGGRIVMQGLVEHAFAALNLRGALIEGGNF
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCCC
GLVSEQEKQQRWISDSHWAKRFREEPIEQVLADWYQQGVFSSLNHAQKQTLIAKRSANLG
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPVAEMLLATSLAKQPNLLPQLQRSSVPLHYVCGEKDTKFQELARQSGLSCSIVDNAGHN
CHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCEEEEECCCCCC
VHQEQPNAFADIVRAFIHSVYQR
CCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA