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The map label for this gene is menD [H]

Identifier: 37679300

GI number: 37679300

Start: 1123887

End: 1125581

Strand: Direct

Name: menD [H]

Synonym: VV1116

Alternate gene names: 37679300

Gene position: 1123887-1125581 (Clockwise)

Preceding gene: 37679299

Following gene: 37679301

Centisome position: 33.5

GC content: 50.91

Gene sequence:

>1695_bases
ATGAACCACGATCAAGCCGTATTGAATCGTATTTGGTGTGAAACGCTGTTTGAAGAATTGTACCGCTTTGGGGTACGAGA
TGTTTGTGTCGCACCTGGCTCACGTTCAACCCCGCTCGCCCTCGAAGCGAATGCCCACACCAGTTTGAAGCTGCATACGC
ATTTTGATGAGCGTGGATTGGGTTTTCTTGCACTTGGTTTAGCCAAAGCCAGCCAGCGGCCTGTGGCGGTGGTGGTGACA
TCAGGCACTGCGGTGGCCAATCTTTTGCCAGCAGTGGCAGAGGCAGGCTTAACCGGGGAGAAACTGGTATTACTGACCGC
AGATCGACCGATCGAGCTGGTGGGATGCGGGGCCAACCAGGCGATCGCACAGCAAGGCATCTTCTCAAATCATGTTTGTG
CTTCATTAAACTTGCCCAGTCCAAACACGCAGACGTCATTAAACTGGTTACTGACATCTGTGGATCAAGTGCTTCATCAA
CAGGCGGTCAGTGGCCATGCGGTGCACATCAACTGTCCCTTTCCTGAGCCGCTTTATTCCAATGCACCAAAGTCGATTTA
CCAGAGTTATATCGATACGGTGGCTGTGTGGCGTGCAGAGGGGGGCATCTACAGCAACAAACAAATGCCGCTCCCCATGC
CGACTTCTATTGCAGAAATTGAACAACGTAAAGCCGTGGTGGTGATTGGCAGTGTAACACTGCAAGAAGCCAAACAGGCC
CATCAATTTGCGGCGCAAATGGGATGGCCTGTGCTGTGTGACCCACAATCCGGAACCACTTCTGATTGGAGTGGTTTTGA
TATCTGGTTGCAAAATCCTGCAGCTCGTGCACAACTGTCTCAATGTGATTTGATCATCCAGTTTGGCCGTCGTTTGGTCT
CGAAGCGTTTACACCAATGGTTAGAGCAGCAAGTGCAAGCTGGCTGCGATTATTGGTACGTTTCACCGGATTTTGAGCGA
GACAACCAGAGCCACCTACCTCAACAGCATTTTGTCTGCTCAATCGCAGCGTGGTTGAACGTGGTGACCAACCGCGAAGT
GCAGCCTGTCGCGTGGGCGAATGAATTGCCGCGTTTTAGTGCGGAGGTGAACAAGCAAGCCAGAGAGATCGCCCAAAGCA
GCCTGTGTGAAATGATGATTGCTCTGCACTTATCCTCACTTGTGGGCAGCGCCGATCTGTTTTTAGGCAATAGCTTGTTT
GTGCGTATGGTCGATATGGTTGGTCAATTGCATGGTGTCGAAACATTTACCAATCGTGGCGCATCGGGGATTGATGGCCT
CTTTGCCACGGCAAGTGGCGTGCAAAGAGCAAGATCCAACCCAATGTTATTGATGATTGGTGACACGTCTGCGTTGTATG
ACTTGAACTCGCTGGCGCTGTATAGCCATCAAGAGACTCCGGTGGTGATCGTGGTGACCAACAACGACGGCGGCGCGATT
TTTGATTTATTACCGGTACCACCGCAGCAAAAACAAGCGTTATACCAAATGCCTCATGGCTACCGTTTCGAATTCGCAGC
CAAACAATTTGGCCTTGATTACGTGCGTCCCACATCGATGACGGAGCTAACAGAACGAATTGTTGGCCATTTTGCACACG
GCTGTGGCGCTTTATTGGTTGAAGTCAACACGCCGCCAAACCAAGCGTCACAGCACATAAAACAATTGGCTGATCATGTC
CGTGCTCTCGTTTAA

Upstream 100 bases:

>100_bases
TTCCTGGCTCAATCGCAGAGCATGAATGGAGTGAACTGGATAAAAAAATGTCGACCTTATTGTCACTGATTTCTGAGCAC
CCACCGCTTGGAGTGGCATC

Downstream 100 bases:

>100_bases
TTATCAGCAAGGCAATGGTGATGACCGTTCGCCCGTGTTGGTATTTTTGCACGGATTATTGGGCAGTGCGGAAGATTGGT
GTGCCACGCGATGTTCACTC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTSLKLHTHFDERGLGFLALGLAKASQRPVAVVVT
SGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQAIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQ
QAVSGHAVHINCPFPEPLYSNAPKSIYQSYIDTVAVWRAEGGIYSNKQMPLPMPTSIAEIEQRKAVVVIGSVTLQEAKQA
HQFAAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQWLEQQVQAGCDYWYVSPDFER
DNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFSAEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLF
VRMVDMVGQLHGVETFTNRGASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVRPTSMTELTERIVGHFAHGCGALLVEVNTPPNQASQHIKQLADHV
RALV

Sequences:

>Translated_564_residues
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTSLKLHTHFDERGLGFLALGLAKASQRPVAVVVT
SGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQAIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQ
QAVSGHAVHINCPFPEPLYSNAPKSIYQSYIDTVAVWRAEGGIYSNKQMPLPMPTSIAEIEQRKAVVVIGSVTLQEAKQA
HQFAAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQWLEQQVQAGCDYWYVSPDFER
DNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFSAEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLF
VRMVDMVGQLHGVETFTNRGASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVRPTSMTELTERIVGHFAHGCGALLVEVNTPPNQASQHIKQLADHV
RALV
>Mature_564_residues
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTSLKLHTHFDERGLGFLALGLAKASQRPVAVVVT
SGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQAIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQ
QAVSGHAVHINCPFPEPLYSNAPKSIYQSYIDTVAVWRAEGGIYSNKQMPLPMPTSIAEIEQRKAVVVIGSVTLQEAKQA
HQFAAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQWLEQQVQAGCDYWYVSPDFER
DNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFSAEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLF
VRMVDMVGQLHGVETFTNRGASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVRPTSMTELTERIVGHFAHGCGALLVEVNTPPNQASQHIKQLADHV
RALV

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=532, Percent_Identity=44.1729323308271, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 61826; Mature: 61826

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTSLKLHTHFDERGL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCEEEEEECCCCCCC
GFLALGLAKASQRPVAVVVTSGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQ
CEEEEHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCH
AIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQQAVSGHAVHINCPFPEPLYS
HHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHC
NAPKSIYQSYIDTVAVWRAEGGIYSNKQMPLPMPTSIAEIEQRKAVVVIGSVTLQEAKQA
CCCHHHHHHHHHHHHHEECCCCEECCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHHHH
HQFAAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQW
HHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQQVQAGCDYWYVSPDFERDNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFS
HHHHHHCCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
AEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLFVRMVDMVGQLHGVETFTNRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCHHHHHCCC
ASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHEEEECCCCCEEEEEECCCCCEE
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVRPTSMTELTERIVGHFAHGCGALLV
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
EVNTPPNQASQHIKQLADHVRALV
EECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHDQAVLNRIWCETLFEELYRFGVRDVCVAPGSRSTPLALEANAHTSLKLHTHFDERGL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCEEEEEECCCCCCC
GFLALGLAKASQRPVAVVVTSGTAVANLLPAVAEAGLTGEKLVLLTADRPIELVGCGANQ
CEEEEHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCH
AIAQQGIFSNHVCASLNLPSPNTQTSLNWLLTSVDQVLHQQAVSGHAVHINCPFPEPLYS
HHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHC
NAPKSIYQSYIDTVAVWRAEGGIYSNKQMPLPMPTSIAEIEQRKAVVVIGSVTLQEAKQA
CCCHHHHHHHHHHHHHEECCCCEECCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHHHH
HQFAAQMGWPVLCDPQSGTTSDWSGFDIWLQNPAARAQLSQCDLIIQFGRRLVSKRLHQW
HHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQQVQAGCDYWYVSPDFERDNQSHLPQQHFVCSIAAWLNVVTNREVQPVAWANELPRFS
HHHHHHCCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
AEVNKQAREIAQSSLCEMMIALHLSSLVGSADLFLGNSLFVRMVDMVGQLHGVETFTNRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCHHHHHCCC
ASGIDGLFATASGVQRARSNPMLLMIGDTSALYDLNSLALYSHQETPVVIVVTNNDGGAI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHEEEECCCCCEEEEEECCCCCEE
FDLLPVPPQQKQALYQMPHGYRFEFAAKQFGLDYVRPTSMTELTERIVGHFAHGCGALLV
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
EVNTPPNQASQHIKQLADHVRALV
EECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA