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The map label for this gene is prfA

Identifier: 37679115

GI number: 37679115

Start: 931352

End: 932443

Strand: Direct

Name: prfA

Synonym: VV0931

Alternate gene names: 37679115

Gene position: 931352-932443 (Clockwise)

Preceding gene: 37679114

Following gene: 37679116

Centisome position: 27.76

GC content: 49.27

Gene sequence:

>1092_bases
ATGATGAAAGCCTCGATTCTAACCAAGCTAGAAATGCTGGTTGAGCGTTATGAAGAAGTGCAGCACCTTCTTGGTGATCC
GGGAGTGATCGGTGACCAAGACAAATTCCGTGCACTGTCAAAAGAGTATTCTCAACTTGAAGAAGTCACTAAGTGCTTTA
CTGCCTATAAACAAGCGCAAGAAGATTTAGTTGCTGCGGAAGAGATGGCCAAAGAAGACGACGCAGAGATGCGTGAAATG
GCGCAAGAAGAAATCAAAGCGGCAAAAGTGGCCATTGAAGATTTGGCTGCAGAGTTGCAAATTCTTCTGCTACCGAAAGA
TCCGAATGACGATCGCAACTGTTTCCTCGAAATTCGTGCGGGCGCAGGCGGTGATGAAGCCGGTATCTTTGCTGGTGATC
TGTTCCGTATGTACAGCAAGTTTGCAGAAAAACGTGGTTGGCGTATTGAAGTCATGTCCGCGAATGAAGCAGAACATGGC
GGCTACAAAGAGATGATCGCGAAAGTGAATGGCGATGGCGCTTACGGTTTCTTGAAGTTTGAGTCGGGCGGTCACCGTGT
TCAACGTGTTCCTGCAACCGAATCGCAAGGTCGCGTACACACCTCGGCGTGTACCGTGGCAATCATGCCTGAAATTCCTG
AAGCGGAAATTCCAGAGATCAAAGCAAGCGATCTAAAAATTGATACTTTCCGCTCTTCTGGTGCGGGCGGTCAGCACGTT
AACACCACCGACTCGGCAATCCGTATTACCCACTTACCAACCGGTACTGTCGTGGAGTGTCAGGACGAGCGTTCACAGCA
CAAAAACAAAGCCAAGGCGATGGCTGTTCTAGCGGCTCGTATTGTTCAAGCTGAAGAGGCTAAGCGTGCAGCTGAAGTGT
CTGATACGCGTCGTAACCTACTTGGTTCGGGCGATCGTAGTGACCGTATTCGTACCTACAACTACCCACAAGGCCGTGTG
TCAGATCACCGCATCAACTTGACGATTTACCGTCTTTCTGAAGTGATGGAAGGCGATCTTCAGTCTTTGATCGATCCTGT
GATTCAGGAACACCAAGCGGACCAATTGGCTGCTTTGGCGGAAAACAACTGA

Upstream 100 bases:

>100_bases
TTGGCCGCAGAGCAGGGTGAGCCCGCAAAATTAACCGTCATTCGCCAAAGCTTAGGGCTGGATGAACTCAAATAATTCTA
TCAATCAGTAAGATATATAA

Downstream 100 bases:

>100_bases
TCCCATGTCTGAGTTGATGACACTAGATGCCGCTGTTAAACAGGCGGCATCTCGCTTTTCTGAGTTAGGCAATGATTCGC
CGTCTTTAGACGCCGCGGTA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQEDLVAAEEMAKEDDAEMREM
AQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHG
GYKEMIAKVNGDGAYGFLKFESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHV
NTTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLLGSGDRSDRIRTYNYPQGRV
SDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAENN

Sequences:

>Translated_363_residues
MMKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQEDLVAAEEMAKEDDAEMREM
AQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHG
GYKEMIAKVNGDGAYGFLKFESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHV
NTTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLLGSGDRSDRIRTYNYPQGRV
SDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAENN
>Mature_363_residues
MMKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQEDLVAAEEMAKEDDAEMREM
AQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHG
GYKEMIAKVNGDGAYGFLKFESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHV
NTTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLLGSGDRSDRIRTYNYPQGRV
SDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAENN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=43.3774834437086, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI34577120, Length=371, Percent_Identity=35.5795148247978, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=42.512077294686, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=72.7777777777778, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=38.7573964497041, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=290, Percent_Identity=35.5172413793103, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=332, Percent_Identity=39.7590361445783, Blast_Score=245, Evalue=6e-66,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=40, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40270; Mature: 40270

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLVAAEEMAKEDDAEMREMAQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEC
GAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHGGYKEMIAKVNGDGAYGFLKF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEE
ESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHV
CCCCCEEEECCCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
NTTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNL
CCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSGDRSDRIRTYNYPQGRVSDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALA
CCCCCCCCCCEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
ENN
CCC
>Mature Secondary Structure
MMKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLVAAEEMAKEDDAEMREMAQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEC
GAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHGGYKEMIAKVNGDGAYGFLKF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEE
ESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHV
CCCCCEEEECCCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
NTTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNL
CCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSGDRSDRIRTYNYPQGRVSDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALA
CCCCCCCCCCEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
ENN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA