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The map label for this gene is hisF3

Identifier: 37678540

GI number: 37678540

Start: 364659

End: 365441

Strand: Direct

Name: hisF3

Synonym: VV0356

Alternate gene names: 37678540

Gene position: 364659-365441 (Clockwise)

Preceding gene: 37678539

Following gene: 37678541

Centisome position: 10.87

GC content: 35.5

Gene sequence:

>783_bases
TTGCGAGTTTATAGAATGTTACGATCTCGAGTTATCCCTGTTTTATTAATGCGTGAAAAAGGCTTGGTAAAAACAGTAAA
GTTTAAAGAGGGAAAATATGTTGGCGATCCTCTTAATGCTGTAAAAATATTTAATGAACAAGAAGCTGATGAGTTAACGT
TACTCGATATTGATGCATCACGTTTAGGTCATGAGCCTGACTATGTGTTAATAGAGCGAATAGCGTCTGAGTGTAGAATG
CCTCTTTGTTATGGCGGTGGTATTAAAACGGTTGAACAGGCTGAGAGAATATTAAAGCTCGGTGTTGAGAAAGTATCATT
AAGTTCCGCGGTGTTTGAAAATCCAAAAATTATCACCCAATTAGCTGAAAGAGTTGGTCGCCAAAGTATTGTTGTTTGTT
TAGATGTTAAGAAAAGGCTATTTGGTTCTAAGTTTGATTGTTTTACTATTAATGGAACAAAAAAACAGTCTGTTGATACT
ATTGAATTTGTCAAACAAATACAGACATTAGGCGCTGGTGAGATAGTTTTAAACTTCATTGATAATGACGGTGTAATGAA
AGGATACGATTTAGACGCTGTTAGTAAGTTTAAAGCGTTGGTAAAAGTACCGTTAACAGTCGTTGGTGGAGCTGGTTGCG
TAGATGATATAGCAAAACTTGTTCAACAAGAAAAATTAGTAGGTGCCGCCGCAGGAAGTTTATTTGTATTTAAAGGTAAA
TACAAAGCAGTGCTTATTAACTATCCATCACCAAGTGAAAAGAAAAAAGCTTTGGAGTTGTAA

Upstream 100 bases:

>100_bases
GGCTTTGAGTACGCTTGCATTGTGAACAAAGATAACGTTTATGGTATCCAATGCCACCCTGAAAAAAGTCACCATAACGG
TGTTGCTTTGTTAAAGAGTT

Downstream 100 bases:

>100_bases
ACACTATTTGGGTTACGAAACGTATTTGAGAGATTAATTACTGATGAAACAAATATTACAAGACATGGCAAAAGGTACCT
CTAATTTAGTTGAGGCACCT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF3; ImGP synthase subunit hisF3; IGPS subunit hisF3

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRVYRMLRSRVIPVLLMREKGLVKTVKFKEGKYVGDPLNAVKIFNEQEADELTLLDIDASRLGHEPDYVLIERIASECRM
PLCYGGGIKTVEQAERILKLGVEKVSLSSAVFENPKIITQLAERVGRQSIVVCLDVKKRLFGSKFDCFTINGTKKQSVDT
IEFVKQIQTLGAGEIVLNFIDNDGVMKGYDLDAVSKFKALVKVPLTVVGGAGCVDDIAKLVQQEKLVGAAAGSLFVFKGK
YKAVLINYPSPSEKKKALEL

Sequences:

>Translated_260_residues
MRVYRMLRSRVIPVLLMREKGLVKTVKFKEGKYVGDPLNAVKIFNEQEADELTLLDIDASRLGHEPDYVLIERIASECRM
PLCYGGGIKTVEQAERILKLGVEKVSLSSAVFENPKIITQLAERVGRQSIVVCLDVKKRLFGSKFDCFTINGTKKQSVDT
IEFVKQIQTLGAGEIVLNFIDNDGVMKGYDLDAVSKFKALVKVPLTVVGGAGCVDDIAKLVQQEKLVGAAAGSLFVFKGK
YKAVLINYPSPSEKKKALEL
>Mature_260_residues
MRVYRMLRSRVIPVLLMREKGLVKTVKFKEGKYVGDPLNAVKIFNEQEADELTLLDIDASRLGHEPDYVLIERIASECRM
PLCYGGGIKTVEQAERILKLGVEKVSLSSAVFENPKIITQLAERVGRQSIVVCLDVKKRLFGSKFDCFTINGTKKQSVDT
IEFVKQIQTLGAGEIVLNFIDNDGVMKGYDLDAVSKFKALVKVPLTVVGGAGCVDDIAKLVQQEKLVGAAAGSLFVFKGK
YKAVLINYPSPSEKKKALEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=234, Percent_Identity=37.1794871794872, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=25.1162790697674, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=228, Percent_Identity=27.6315789473684, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS63_VIBVY (Q7MPK7)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_933149.1
- ProteinModelPortal:   Q7MPK7
- SMR:   Q7MPK7
- STRING:   Q7MPK7
- GeneID:   2623123
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0356
- NMPDR:   fig|196600.1.peg.424
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   TIENSSI
- ProtClustDB:   PRK01033
- BioCyc:   VVUL196600:VV0356-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28730; Mature: 28730

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYRMLRSRVIPVLLMREKGLVKTVKFKEGKYVGDPLNAVKIFNEQEADELTLLDIDAS
CHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECHH
RLGHEPDYVLIERIASECRMPLCYGGGIKTVEQAERILKLGVEKVSLSSAVFENPKIITQ
HCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHH
LAERVGRQSIVVCLDVKKRLFGSKFDCFTINGTKKQSVDTIEFVKQIQTLGAGEIVLNFI
HHHHHCCCEEEEEEECHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
DNDGVMKGYDLDAVSKFKALVKVPLTVVGGAGCVDDIAKLVQQEKLVGAAAGSLFVFKGK
CCCCCEECCCHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
YKAVLINYPSPSEKKKALEL
EEEEEEECCCCCHHHHCCCC
>Mature Secondary Structure
MRVYRMLRSRVIPVLLMREKGLVKTVKFKEGKYVGDPLNAVKIFNEQEADELTLLDIDAS
CHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECHH
RLGHEPDYVLIERIASECRMPLCYGGGIKTVEQAERILKLGVEKVSLSSAVFENPKIITQ
HCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHH
LAERVGRQSIVVCLDVKKRLFGSKFDCFTINGTKKQSVDTIEFVKQIQTLGAGEIVLNFI
HHHHHCCCEEEEEEECHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
DNDGVMKGYDLDAVSKFKALVKVPLTVVGGAGCVDDIAKLVQQEKLVGAAAGSLFVFKGK
CCCCCEECCCHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
YKAVLINYPSPSEKKKALEL
EEEEEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA