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The map label for this gene is hisH

Identifier: 37678539

GI number: 37678539

Start: 364052

End: 364672

Strand: Direct

Name: hisH

Synonym: VV0355

Alternate gene names: 37678539

Gene position: 364052-364672 (Clockwise)

Preceding gene: 37678538

Following gene: 37678540

Centisome position: 10.85

GC content: 37.84

Gene sequence:

>621_bases
ATGATTACTATCGTTGATTATGGTCTTGGTAATATCAAGGCATTTGCTAACTTATACAAGAAACTGAATATTGAGTTAGT
GTTTGCTAGTGATGTAAATGACCTTGCTAACGCAACCAAGATTATTTTACCTGGTGTAGGAGCATTTGATCATGCTATGC
AAATGCTTAATGACTCAGGTATGCGCGAGACATTAGATGAACTAGTGTTAGAAAAAAAGGTACCTGTAATTGGCATATGT
GTTGGTATGCAAATGATGGCTGACTCCAGCGAAGAGGGGCAATTAAAAGGACTCGGATGGATACCTGGTACAGTTAAAAA
GTTTAAAAGAGAGTCTCAAGCTCTAGAAAGTCAATATCCGCTACCGCATATGGGATGGAATTCACTTGAAATAGCAAGAA
CATCTGAGTTGTTGTTAGGCTTGGACGAGCAAAAACTTTTTTATTTTTTGCATTCTTATTATTACGAGCCAAACGATAAA
GAACATGTGGTCGCCACTGCAAATTATGGCTTTGAGTACGCTTGCATTGTGAACAAAGATAACGTTTATGGTATCCAATG
CCACCCTGAAAAAAGTCACCATAACGGTGTTGCTTTGTTAAAGAGTTTTGCGAGTTTATAG

Upstream 100 bases:

>100_bases
AACCGAACAAAACATACAAAGATTACAATAATCAAGAGTCTATGTTTGAGTTTGGAGCGAAAGTATTGCGATGGTTAGGT
GTTGAGAGGTCTATTAAACG

Downstream 100 bases:

>100_bases
AATGTTACGATCTCGAGTTATCCCTGTTTTATTAATGCGTGAAAAAGGCTTGGTAAAAACAGTAAAGTTTAAAGAGGGAA
AATATGTTGGCGATCCTCTT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 3; IGP synthase subunit hisH 3; ImGP synthase subunit hisH 3; IGPS subunit hisH 3

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MITIVDYGLGNIKAFANLYKKLNIELVFASDVNDLANATKIILPGVGAFDHAMQMLNDSGMRETLDELVLEKKVPVIGIC
VGMQMMADSSEEGQLKGLGWIPGTVKKFKRESQALESQYPLPHMGWNSLEIARTSELLLGLDEQKLFYFLHSYYYEPNDK
EHVVATANYGFEYACIVNKDNVYGIQCHPEKSHHNGVALLKSFASL

Sequences:

>Translated_206_residues
MITIVDYGLGNIKAFANLYKKLNIELVFASDVNDLANATKIILPGVGAFDHAMQMLNDSGMRETLDELVLEKKVPVIGIC
VGMQMMADSSEEGQLKGLGWIPGTVKKFKRESQALESQYPLPHMGWNSLEIARTSELLLGLDEQKLFYFLHSYYYEPNDK
EHVVATANYGFEYACIVNKDNVYGIQCHPEKSHHNGVALLKSFASL
>Mature_206_residues
MITIVDYGLGNIKAFANLYKKLNIELVFASDVNDLANATKIILPGVGAFDHAMQMLNDSGMRETLDELVLEKKVPVIGIC
VGMQMMADSSEEGQLKGLGWIPGTVKKFKRESQALESQYPLPHMGWNSLEIARTSELLLGLDEQKLFYFLHSYYYEPNDK
EHVVATANYGFEYACIVNKDNVYGIQCHPEKSHHNGVALLKSFASL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=203, Percent_Identity=35.4679802955665, Blast_Score=114, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=215, Percent_Identity=30.6976744186047, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS53_VIBVY (Q7MPK8)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_933148.1
- ProteinModelPortal:   Q7MPK8
- SMR:   Q7MPK8
- STRING:   Q7MPK8
- GeneID:   2623122
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0355
- NMPDR:   fig|196600.1.peg.423
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   ATKIILP
- ProtClustDB:   PRK13181
- BioCyc:   VVUL196600:VV0355-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23036; Mature: 23036

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 188-188 ACT_SITE 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIVDYGLGNIKAFANLYKKLNIELVFASDVNDLANATKIILPGVGAFDHAMQMLNDSG
CEEEEECCCCHHHHHHHHHHHCCEEEEEECCCHHHHCCCEEEECCCCHHHHHHHHHCCCC
MRETLDELVLEKKVPVIGICVGMQMMADSSEEGQLKGLGWIPGTVKKFKRESQALESQYP
HHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
LPHMGWNSLEIARTSELLLGLDEQKLFYFLHSYYYEPNDKEHVVATANYGFEYACIVNKD
CCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECC
NVYGIQCHPEKSHHNGVALLKSFASL
CEEEEEECCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MITIVDYGLGNIKAFANLYKKLNIELVFASDVNDLANATKIILPGVGAFDHAMQMLNDSG
CEEEEECCCCHHHHHHHHHHHCCEEEEEECCCHHHHCCCEEEECCCCHHHHHHHHHCCCC
MRETLDELVLEKKVPVIGICVGMQMMADSSEEGQLKGLGWIPGTVKKFKRESQALESQYP
HHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
LPHMGWNSLEIARTSELLLGLDEQKLFYFLHSYYYEPNDKEHVVATANYGFEYACIVNKD
CCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECC
NVYGIQCHPEKSHHNGVALLKSFASL
CEEEEEECCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA