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The map label for this gene is rfbK [H]

Identifier: 37678537

GI number: 37678537

Start: 361498

End: 362907

Strand: Direct

Name: rfbK [H]

Synonym: VV0353

Alternate gene names: 37678537

Gene position: 361498-362907 (Clockwise)

Preceding gene: 37678536

Following gene: 37678538

Centisome position: 10.78

GC content: 40.92

Gene sequence:

>1410_bases
ATGAATACAAAGGCAGTAATTCAAACAAGTGGCATTGCATTTGGTACCAGTGGTGCTAGAGGCCTCGTTGTCGACTTTAC
ACCAGAAGTGTGTGCTGCTTTTTCAATTGCATTTGCTCAGAGTATTAAAAGCGAATTCGTTTTTAATACAATGGCCATTG
CTATTGATAATCGCCCGAGCAGTTTTGATATCGCCAAAGCCTGCGCAGCAGGTTTAAAGCAGCTAAATATAGACGTTGTG
TATTACGGTGTGGTACCAACTCCAGCGCTTGCTTATAAAGCGATGCAAGACAATATGCCTTGTATTATGGTTACAGGTAG
TCATATTCCATTTGATAGAAATGGCTTAAAGTTTTACCGCCCTGATGGTGAAATTACCAAGCAAGATGAGCAAGCTATTT
TATCTGCTGATTTTGATTTTGCTGACTTGAACCTAGTTGATATCAGTTTACCAACAAGTGCTAAAGCAAAAGATGCTTAT
ATTGCTCGATATACAAACCTGTTTAACGAAGAGTCGTTATCCGGTAAAAGAGTAGGCATTTATGAGCACTCGAGTGCAGG
TAGAGATATTTATAAATCACTATTTGAGCAACTAGGCGCAGAAGTTATTTGCCTAGGGCGCAGTGACGAATTTATCCCTA
TCGATACCGAGGCTGTATCAGATGAAGATAAGCTAAGAGCCAAAAAGTGGGTAAGCGAATATCATTTAGATATGCTGTTT
TCAACTGACGGTGATGGTGACAGACCGCTCGTTGCAGGCGAGGATGGGGAGTGGTTAAGGGGGGATATTTTAGGCCTCCT
TTGTAGTCAAGCACTCTCAGTAGGAGCGCTTGCAACACCAATTAGCTGTAATACAGCCATTGAGTTGTCAGGTAGCTTTA
AAACAGTGACTAGAACAAAGATTGGCTCACCTTATGTAATAGAAGCCTTTGCAGAGTTAAACAAAACTTACAAGCGAGTA
GCGGGTTTTGAAGCAAATGGTGGCTACTTATTAGCGAGTGACTTTGAATTTGACGGAAAAGTACTGAAAGCACTGCCAAC
AAGAGATGCTGTGTTACCTGCACTTATAGTAGCAACAATGGGTGATAGTATAGCAAGTTTGCTAGCAGCATTACCTCAAA
GGTTTACAGCAAGTGATAGGCTTAAGGATTTTGCAACAGAAAAGGGCAAAGCGCTAATAGCGGGACTAAATGCAAACCCC
AATGCTATTTTGAAGAGCTTAGGTTTAGGTGAAGCAAGGTCCGTTAGTAAAATAGATGGTTTAAGGTTGACTGCAGAGAA
CGGCGATATAGTTCACCTAAGGACTTCTGGTAATGCGCCTGAGCTTAGATGCTACGTTGAAAGTGGTTGTCTTGAAAGTT
TAGAAGTGCTCTTAAAATCTGTTTTGAAAAATATTGAAGAAATGCAATGA

Upstream 100 bases:

>100_bases
AGTAGATTTAGAGCTTATTGAGGTTCAGTCTGGTTCGTACTTAGGCGAAGATGATATTGTGCGCTTTGAAGACCGCTATG
GAAGGGTAAAAGATTAAGTT

Downstream 100 bases:

>100_bases
TTATTATGACTAAAAATAGAGAAAACCAAATCTGTACTAATTGTGTTATGGACACAACTGACTCAAAAATTACATTTGAC
TCAAAAGGTGTTTGCGATCA

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MNTKAVIQTSGIAFGTSGARGLVVDFTPEVCAAFSIAFAQSIKSEFVFNTMAIAIDNRPSSFDIAKACAAGLKQLNIDVV
YYGVVPTPALAYKAMQDNMPCIMVTGSHIPFDRNGLKFYRPDGEITKQDEQAILSADFDFADLNLVDISLPTSAKAKDAY
IARYTNLFNEESLSGKRVGIYEHSSAGRDIYKSLFEQLGAEVICLGRSDEFIPIDTEAVSDEDKLRAKKWVSEYHLDMLF
STDGDGDRPLVAGEDGEWLRGDILGLLCSQALSVGALATPISCNTAIELSGSFKTVTRTKIGSPYVIEAFAELNKTYKRV
AGFEANGGYLLASDFEFDGKVLKALPTRDAVLPALIVATMGDSIASLLAALPQRFTASDRLKDFATEKGKALIAGLNANP
NAILKSLGLGEARSVSKIDGLRLTAENGDIVHLRTSGNAPELRCYVESGCLESLEVLLKSVLKNIEEMQ

Sequences:

>Translated_469_residues
MNTKAVIQTSGIAFGTSGARGLVVDFTPEVCAAFSIAFAQSIKSEFVFNTMAIAIDNRPSSFDIAKACAAGLKQLNIDVV
YYGVVPTPALAYKAMQDNMPCIMVTGSHIPFDRNGLKFYRPDGEITKQDEQAILSADFDFADLNLVDISLPTSAKAKDAY
IARYTNLFNEESLSGKRVGIYEHSSAGRDIYKSLFEQLGAEVICLGRSDEFIPIDTEAVSDEDKLRAKKWVSEYHLDMLF
STDGDGDRPLVAGEDGEWLRGDILGLLCSQALSVGALATPISCNTAIELSGSFKTVTRTKIGSPYVIEAFAELNKTYKRV
AGFEANGGYLLASDFEFDGKVLKALPTRDAVLPALIVATMGDSIASLLAALPQRFTASDRLKDFATEKGKALIAGLNANP
NAILKSLGLGEARSVSKIDGLRLTAENGDIVHLRTSGNAPELRCYVESGCLESLEVLLKSVLKNIEEMQ
>Mature_469_residues
MNTKAVIQTSGIAFGTSGARGLVVDFTPEVCAAFSIAFAQSIKSEFVFNTMAIAIDNRPSSFDIAKACAAGLKQLNIDVV
YYGVVPTPALAYKAMQDNMPCIMVTGSHIPFDRNGLKFYRPDGEITKQDEQAILSADFDFADLNLVDISLPTSAKAKDAY
IARYTNLFNEESLSGKRVGIYEHSSAGRDIYKSLFEQLGAEVICLGRSDEFIPIDTEAVSDEDKLRAKKWVSEYHLDMLF
STDGDGDRPLVAGEDGEWLRGDILGLLCSQALSVGALATPISCNTAIELSGSFKTVTRTKIGSPYVIEAFAELNKTYKRV
AGFEANGGYLLASDFEFDGKVLKALPTRDAVLPALIVATMGDSIASLLAALPQRFTASDRLKDFATEKGKALIAGLNANP
NAILKSLGLGEARSVSKIDGLRLTAENGDIVHLRTSGNAPELRCYVESGCLESLEVLLKSVLKNIEEMQ

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1789566, Length=317, Percent_Identity=28.7066246056782, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788361, Length=449, Percent_Identity=24.0534521158129, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 50582; Mature: 50582

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKAVIQTSGIAFGTSGARGLVVDFTPEVCAAFSIAFAQSIKSEFVFNTMAIAIDNRPS
CCCCEEEEECCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
SFDIAKACAAGLKQLNIDVVYYGVVPTPALAYKAMQDNMPCIMVTGSHIPFDRNGLKFYR
CCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEC
PDGEITKQDEQAILSADFDFADLNLVDISLPTSAKAKDAYIARYTNLFNEESLSGKRVGI
CCCCCCCCCCHHEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEE
YEHSSAGRDIYKSLFEQLGAEVICLGRSDEFIPIDTEAVSDEDKLRAKKWVSEYHLDMLF
EECCCCCHHHHHHHHHHCCCEEEEECCCCCEEECCHHHCCCHHHHHHHHHHHHHCEEEEE
STDGDGDRPLVAGEDGEWLRGDILGLLCSQALSVGALATPISCNTAIELSGSFKTVTRTK
ECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCEEEECCCEEEEEHHC
IGSPYVIEAFAELNKTYKRVAGFEANGGYLLASDFEFDGKVLKALPTRDAVLPALIVATM
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHH
GDSIASLLAALPQRFTASDRLKDFATEKGKALIAGLNANPNAILKSLGLGEARSVSKIDG
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHCCC
LRLTAENGDIVHLRTSGNAPELRCYVESGCLESLEVLLKSVLKNIEEMQ
EEEEECCCCEEEEEECCCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTKAVIQTSGIAFGTSGARGLVVDFTPEVCAAFSIAFAQSIKSEFVFNTMAIAIDNRPS
CCCCEEEEECCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
SFDIAKACAAGLKQLNIDVVYYGVVPTPALAYKAMQDNMPCIMVTGSHIPFDRNGLKFYR
CCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEC
PDGEITKQDEQAILSADFDFADLNLVDISLPTSAKAKDAYIARYTNLFNEESLSGKRVGI
CCCCCCCCCCHHEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEE
YEHSSAGRDIYKSLFEQLGAEVICLGRSDEFIPIDTEAVSDEDKLRAKKWVSEYHLDMLF
EECCCCCHHHHHHHHHHCCCEEEEECCCCCEEECCHHHCCCHHHHHHHHHHHHHCEEEEE
STDGDGDRPLVAGEDGEWLRGDILGLLCSQALSVGALATPISCNTAIELSGSFKTVTRTK
ECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCEEEECCCEEEEEHHC
IGSPYVIEAFAELNKTYKRVAGFEANGGYLLASDFEFDGKVLKALPTRDAVLPALIVATM
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHH
GDSIASLLAALPQRFTASDRLKDFATEKGKALIAGLNANPNAILKSLGLGEARSVSKIDG
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHCCC
LRLTAENGDIVHLRTSGNAPELRCYVESGCLESLEVLLKSVLKNIEEMQ
EEEEECCCCEEEEEECCCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]