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The map label for this gene is manC [H]

Identifier: 37678536

GI number: 37678536

Start: 360085

End: 361494

Strand: Direct

Name: manC [H]

Synonym: VV0352

Alternate gene names: 37678536

Gene position: 360085-361494 (Clockwise)

Preceding gene: 37678535

Following gene: 37678537

Centisome position: 10.73

GC content: 41.49

Gene sequence:

>1410_bases
ATGATATTACCAATCATTATGGCAGGCGGTTCAGGCACGCGTTTATGGCCATTGTCGCGTGATAACTACCCAAAACAATT
TTTAAAATTGCATGGTGAAAATACTATGCTGCAAGAGACTATTTTAAGATTAAATGGTCTTGAGCACTCACCTGCGATGT
TGATTTGTAACGAAGAACATCGTTTTATTGCAGCAGAGCAAGTTCGTCAAATTGGTGTTGCACATAGTGGCATCTTTTTA
GAACCCGTGGGTCGCAATACCGCACCAGCCATTGCATTAGCAGCTTTTAAAGCAGTTGAAAACAATCAAGATGCATTGCT
GCTTGTTTTGGCAGCAGACCATGTTATTGAAAATCAAGCAGCGTTCCAAGAAAGTGTTAACAACGCAAAGTTGTTGGCAG
AGCAAGGTAAACTCGTGACCTTTGGTATCGTTGGTAATACGCCTGAAACGGCTTATGGCTATATTAAACGTGGAAAAGAA
GTTGAACATGGCTTTGTGGTTGATAGCTTTGTAGAAAAACCAGACCTTCAAACAGCACAAGAATATATTGCCAGTGGCAA
TTACTTCTGGAATAGCGGCATGTTCTTGTTCAAGGCAAGCCGTTATCTTGAAGAGCTTAAAGCATACCGCCCAGACATTT
ATGAAGCGTGTGAAAAAGCTGTTCAAGTACAAAATAATGACATTGATTTTGTGCGTGTAGATAAAGACGCATTTGAAGCA
TGCCCAGATGAATCCGTTGACTATGCAGTCATGGAGCATACACAGGATGCTGTTGTTGTGCCGATGAATGCAAACTGGAA
TGATGTTGGCGGCTTTGCAGCATTATGGGAAGTGTCTGATCAAGATGAAAATGGCAACGCTTTTGTCGGCGACGTTAAAT
CAGTTGATACAAAAAACACCCTTGTGTTTGCTAAAGACAAGTTGGTTACAACAGTTGGTGTTGAAGACTTAGTTATCGTT
AATACCAAGGATGCTGTATTAGTTGCGCACAAAGACGAATCTCAAAAAGTAAAAGAAATCGTTAGCCAATTAAAAGCGGA
CGAGCGTTCAGAAGTGACATTCCACCGTGAAGTATATCGCCCTTGGGGTAAATACGATTCGGTAGATAACGGAGAGCGCT
TCCAAGTTAAACGTATTACGGTTAAGCCTGGCGCAAAACTATCCGTGCAAATGCATCATCACAGAGCTGAGCATTGGATT
GTGGTTTCTGGTACAGCAAAAGTACAAATTGATGATACAGAGCAGTTCGTCACTGAAAATGAATCAGTATACATTCCTAT
CACCGCGGTTCACGCACTAGAAAATCCAGGCAAAGTAGATTTAGAGCTTATTGAGGTTCAGTCTGGTTCGTACTTAGGCG
AAGATGATATTGTGCGCTTTGAAGACCGCTATGGAAGGGTAAAAGATTAA

Upstream 100 bases:

>100_bases
ATCAATGGTTTGATATAGATAGCCTATTAATAGATGAAAAAGTCCATAAGCATACAAAATGGTACTTTGAAGCATTGCAG
AATACACAGGGACAATTAAT

Downstream 100 bases:

>100_bases
GTTATGAATACAAAGGCAGTAATTCAAACAAGTGGCATTGCATTTGGTACCAGTGGTGCTAGAGGCCTCGTTGTCGACTT
TACACCAGAAGTGTGTGCTG

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MILPIIMAGGSGTRLWPLSRDNYPKQFLKLHGENTMLQETILRLNGLEHSPAMLICNEEHRFIAAEQVRQIGVAHSGIFL
EPVGRNTAPAIALAAFKAVENNQDALLLVLAADHVIENQAAFQESVNNAKLLAEQGKLVTFGIVGNTPETAYGYIKRGKE
VEHGFVVDSFVEKPDLQTAQEYIASGNYFWNSGMFLFKASRYLEELKAYRPDIYEACEKAVQVQNNDIDFVRVDKDAFEA
CPDESVDYAVMEHTQDAVVVPMNANWNDVGGFAALWEVSDQDENGNAFVGDVKSVDTKNTLVFAKDKLVTTVGVEDLVIV
NTKDAVLVAHKDESQKVKEIVSQLKADERSEVTFHREVYRPWGKYDSVDNGERFQVKRITVKPGAKLSVQMHHHRAEHWI
VVSGTAKVQIDDTEQFVTENESVYIPITAVHALENPGKVDLELIEVQSGSYLGEDDIVRFEDRYGRVKD

Sequences:

>Translated_469_residues
MILPIIMAGGSGTRLWPLSRDNYPKQFLKLHGENTMLQETILRLNGLEHSPAMLICNEEHRFIAAEQVRQIGVAHSGIFL
EPVGRNTAPAIALAAFKAVENNQDALLLVLAADHVIENQAAFQESVNNAKLLAEQGKLVTFGIVGNTPETAYGYIKRGKE
VEHGFVVDSFVEKPDLQTAQEYIASGNYFWNSGMFLFKASRYLEELKAYRPDIYEACEKAVQVQNNDIDFVRVDKDAFEA
CPDESVDYAVMEHTQDAVVVPMNANWNDVGGFAALWEVSDQDENGNAFVGDVKSVDTKNTLVFAKDKLVTTVGVEDLVIV
NTKDAVLVAHKDESQKVKEIVSQLKADERSEVTFHREVYRPWGKYDSVDNGERFQVKRITVKPGAKLSVQMHHHRAEHWI
VVSGTAKVQIDDTEQFVTENESVYIPITAVHALENPGKVDLELIEVQSGSYLGEDDIVRFEDRYGRVKD
>Mature_469_residues
MILPIIMAGGSGTRLWPLSRDNYPKQFLKLHGENTMLQETILRLNGLEHSPAMLICNEEHRFIAAEQVRQIGVAHSGIFL
EPVGRNTAPAIALAAFKAVENNQDALLLVLAADHVIENQAAFQESVNNAKLLAEQGKLVTFGIVGNTPETAYGYIKRGKE
VEHGFVVDSFVEKPDLQTAQEYIASGNYFWNSGMFLFKASRYLEELKAYRPDIYEACEKAVQVQNNDIDFVRVDKDAFEA
CPDESVDYAVMEHTQDAVVVPMNANWNDVGGFAALWEVSDQDENGNAFVGDVKSVDTKNTLVFAKDKLVTTVGVEDLVIV
NTKDAVLVAHKDESQKVKEIVSQLKADERSEVTFHREVYRPWGKYDSVDNGERFQVKRITVKPGAKLSVQMHHHRAEHWI
VVSGTAKVQIDDTEQFVTENESVYIPITAVHALENPGKVDLELIEVQSGSYLGEDDIVRFEDRYGRVKD

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=474, Percent_Identity=63.7130801687764, Blast_Score=601, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR018247
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52484; Mature: 52484

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPIIMAGGSGTRLWPLSRDNYPKQFLKLHGENTMLQETILRLNGLEHSPAMLICNEEH
CEEEEEEECCCCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
RFIAAEQVRQIGVAHSGIFLEPVGRNTAPAIALAAFKAVENNQDALLLVLAADHVIENQA
CEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
AFQESVNNAKLLAEQGKLVTFGIVGNTPETAYGYIKRGKEVEHGFVVDSFVEKPDLQTAQ
HHHHCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEHHHHCCCCHHHHH
EYIASGNYFWNSGMFLFKASRYLEELKAYRPDIYEACEKAVQVQNNDIDFVRVDKDAFEA
HHHHCCCEEECCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECHHHHHC
CPDESVDYAVMEHTQDAVVVPMNANWNDVGGFAALWEVSDQDENGNAFVGDVKSVDTKNT
CCCCCCCCCEEECCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCE
LVFAKDKLVTTVGVEDLVIVNTKDAVLVAHKDESQKVKEIVSQLKADERSEVTFHREVYR
EEEEECCEEEEECCCEEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEHHHHC
PWGKYDSVDNGERFQVKRITVKPGAKLSVQMHHHRAEHWIVVSGTAKVQIDDTEQFVTEN
CCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCEEEEEECEEEEEECCCHHHHCCC
ESVYIPITAVHALENPGKVDLELIEVQSGSYLGEDDIVRFEDRYGRVKD
CCEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEEHHCCCCCCC
>Mature Secondary Structure
MILPIIMAGGSGTRLWPLSRDNYPKQFLKLHGENTMLQETILRLNGLEHSPAMLICNEEH
CEEEEEEECCCCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
RFIAAEQVRQIGVAHSGIFLEPVGRNTAPAIALAAFKAVENNQDALLLVLAADHVIENQA
CEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
AFQESVNNAKLLAEQGKLVTFGIVGNTPETAYGYIKRGKEVEHGFVVDSFVEKPDLQTAQ
HHHHCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEHHHHCCCCHHHHH
EYIASGNYFWNSGMFLFKASRYLEELKAYRPDIYEACEKAVQVQNNDIDFVRVDKDAFEA
HHHHCCCEEECCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECHHHHHC
CPDESVDYAVMEHTQDAVVVPMNANWNDVGGFAALWEVSDQDENGNAFVGDVKSVDTKNT
CCCCCCCCCEEECCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCE
LVFAKDKLVTTVGVEDLVIVNTKDAVLVAHKDESQKVKEIVSQLKADERSEVTFHREVYR
EEEEECCEEEEECCCEEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEHHHHC
PWGKYDSVDNGERFQVKRITVKPGAKLSVQMHHHRAEHWIVVSGTAKVQIDDTEQFVTEN
CCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCEEEEEECEEEEEECCCHHHHCCC
ESVYIPITAVHALENPGKVDLELIEVQSGSYLGEDDIVRFEDRYGRVKD
CCEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]