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The map label for this gene is murD

Identifier: 37523301

GI number: 37523301

Start: 3942301

End: 3943626

Strand: Reverse

Name: murD

Synonym: gvip505

Alternate gene names: 37523301

Gene position: 3943626-3942301 (Counterclockwise)

Preceding gene: 37523304

Following gene: 37523300

Centisome position: 84.64

GC content: 66.59

Gene sequence:

>1326_bases
ATGGCGGCGGTAGCGGTGGTCGGAGCGGGCAAATCCGGGCAGGCGGCGGCGCGGTGGTTGGCGCTTGGGGGAAGACGGGT
AGTGCTCTGGGATGGCCGCGACAGCGAGGCATTGCGGGTAGTTGCCGGGGCATTGGCTCCGTTCGGTGTCGAGGCGGTGC
TCGGCCGGGAATTCGTGCCCGAGGAGCCGGATTTGAGCCTGGTGGTGGTGAGCCCCGGGGTGCGTTGGGATCATCCGGGC
TTGGTGGCGGCGCGGGCGCGGGGAGTCACGGTGACCGGCGAGGTGGGCCTTGCCTGGGAATCGCTCTCGCATCGCCGGTG
GCTTTGTGTGACGGGCACCAACGGCAAGACGACCACCACCGCCCTGGTTGGCCACATCCTCAAGACGGCCGGTTTGCGGG
CACCGGTCTGCGGCAACATCGGCCGTCCCGTCACCGACTTGCTGCTGGAGCCCGAAGATTACGACTGGATCGTCGCTGAA
TTGAGCAGCTTTCAGATCGAAAGCGCTCAGGGGATACGCCCGGAGGTGGCGGTGTGGACGACCTTTACTCCCGACCACCT
CAATCGCCACGGTACCCTGGAGCGCTACGCGGCGATCAAAGCCGGGTTGCTGATGCAAGCCCGGCGCGCCGTGCTCAACG
GTGACGACGCCTACCTGGGGGCCCGACGCTCCGCCTGGCCCGACGCCTGGTGGACGTCTACGCAAGCTCCGGCGGCGGTC
AGTCTCGCCGGGAAAGACATTTGCATCGAAAACCGGCCTGTACTGCCGGTGAGCGCCGTGCGCCTGCCCGGGGCGCACAA
CTTGCAAAACGTGCTGATGGCCGTGGCCGCCTGTCATCTGACGGGAGTCGGCGACGCAGCGATTGCCTCGGGTGTGGCGA
GCTTTATAGGGGTGCCCCATCGCCTTGAAGCAGTGGGCGAATACCGGGGAGTGCGCTTTATCAACGATTCGAAGGCCACC
AACTACGACGCCGCTCTGGTAGGGCTCACCGCCGTCCCGGCCCCGAGCGTGCTCATCGCCGGCGGTCAGGCCAAAACCGG
CGAAAGCGGACCGTGGCTCCGGGCCATCGCCGAGCGCTGTGCGTCCGTGGTCCTCATCGGCGAAGCGGCGCCGCTGTTTG
AAAAGTGGCTTCGAGCACAAGATTATCGGGCCGTCTACACAGCCCATACCCTGGAGCGGGCGGTGCCTATGGCTTTCGAG
CAGGCCCGCGCCCAAGGGGCACAGTGCGTGCTGTTTTCACCCGCCTGCGCCAGCTTCGATCAATTTCGCAATTTTGAGGA
GCGGGGCGATCGCTTCCGCGCCCTGATCGCCGCGCTCGCAAGCTAG

Upstream 100 bases:

>100_bases
GATTACAAATTTAGAATAGATCTAATTTGAGAGTGTGTCAAGATCGGTTGGCAAAAATACCCGTACACTGGCGTGGCGAT
GGTGCGAGGTGGTGCCCTTT

Downstream 100 bases:

>100_bases
GACGGGTCGCGCTCGATGATAAAAAGCAATGCCGCCGCCGCCATAAAGGGCACCACCAGGGTGGTGATGATCACCAGGGC
CGGGAACACGTTGTAGGACT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAAVAVVGAGKSGQAAARWLALGGRRVVLWDGRDSEALRVVAGALAPFGVEAVLGREFVPEEPDLSLVVVSPGVRWDHPG
LVAARARGVTVTGEVGLAWESLSHRRWLCVTGTNGKTTTTALVGHILKTAGLRAPVCGNIGRPVTDLLLEPEDYDWIVAE
LSSFQIESAQGIRPEVAVWTTFTPDHLNRHGTLERYAAIKAGLLMQARRAVLNGDDAYLGARRSAWPDAWWTSTQAPAAV
SLAGKDICIENRPVLPVSAVRLPGAHNLQNVLMAVAACHLTGVGDAAIASGVASFIGVPHRLEAVGEYRGVRFINDSKAT
NYDAALVGLTAVPAPSVLIAGGQAKTGESGPWLRAIAERCASVVLIGEAAPLFEKWLRAQDYRAVYTAHTLERAVPMAFE
QARAQGAQCVLFSPACASFDQFRNFEERGDRFRALIAALAS

Sequences:

>Translated_441_residues
MAAVAVVGAGKSGQAAARWLALGGRRVVLWDGRDSEALRVVAGALAPFGVEAVLGREFVPEEPDLSLVVVSPGVRWDHPG
LVAARARGVTVTGEVGLAWESLSHRRWLCVTGTNGKTTTTALVGHILKTAGLRAPVCGNIGRPVTDLLLEPEDYDWIVAE
LSSFQIESAQGIRPEVAVWTTFTPDHLNRHGTLERYAAIKAGLLMQARRAVLNGDDAYLGARRSAWPDAWWTSTQAPAAV
SLAGKDICIENRPVLPVSAVRLPGAHNLQNVLMAVAACHLTGVGDAAIASGVASFIGVPHRLEAVGEYRGVRFINDSKAT
NYDAALVGLTAVPAPSVLIAGGQAKTGESGPWLRAIAERCASVVLIGEAAPLFEKWLRAQDYRAVYTAHTLERAVPMAFE
QARAQGAQCVLFSPACASFDQFRNFEERGDRFRALIAALAS
>Mature_440_residues
AAVAVVGAGKSGQAAARWLALGGRRVVLWDGRDSEALRVVAGALAPFGVEAVLGREFVPEEPDLSLVVVSPGVRWDHPGL
VAARARGVTVTGEVGLAWESLSHRRWLCVTGTNGKTTTTALVGHILKTAGLRAPVCGNIGRPVTDLLLEPEDYDWIVAEL
SSFQIESAQGIRPEVAVWTTFTPDHLNRHGTLERYAAIKAGLLMQARRAVLNGDDAYLGARRSAWPDAWWTSTQAPAAVS
LAGKDICIENRPVLPVSAVRLPGAHNLQNVLMAVAACHLTGVGDAAIASGVASFIGVPHRLEAVGEYRGVRFINDSKATN
YDAALVGLTAVPAPSVLIAGGQAKTGESGPWLRAIAERCASVVLIGEAAPLFEKWLRAQDYRAVYTAHTLERAVPMAFEQ
ARAQGAQCVLFSPACASFDQFRNFEERGDRFRALIAALAS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=388, Percent_Identity=34.5360824742268, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1786274, Length=291, Percent_Identity=30.5841924398625, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_GLOVI (Q7NEZ5)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926678.1
- ProteinModelPortal:   Q7NEZ5
- SMR:   Q7NEZ5
- GeneID:   2602136
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3732
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02705
- BioCyc:   GVIO251221:GLL3732-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46888; Mature: 46757

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVAVVGAGKSGQAAARWLALGGRRVVLWDGRDSEALRVVAGALAPFGVEAVLGREFVP
CCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCCCC
EEPDLSLVVVSPGVRWDHPGLVAARARGVTVTGEVGLAWESLSHRRWLCVTGTNGKTTTT
CCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCCCCCHHHCCCCEEEEEECCCCCHHHH
ALVGHILKTAGLRAPVCGNIGRPVTDLLLEPEDYDWIVAELSSFQIESAQGIRPEVAVWT
HHHHHHHHHCCCCCCCCCCCCCCHHHHEECCCCCCCCEEEHHHHEECCCCCCCCCEEEEE
TFTPDHLNRHGTLERYAAIKAGLLMQARRAVLNGDDAYLGARRSAWPDAWWTSTQAPAAV
EECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCEE
SLAGKDICIENRPVLPVSAVRLPGAHNLQNVLMAVAACHLTGVGDAAIASGVASFIGVPH
EECCCEEEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
RLEAVGEYRGVRFINDSKATNYDAALVGLTAVPAPSVLIAGGQAKTGESGPWLRAIAERC
HHHHHHHHCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHH
ASVVLIGEAAPLFEKWLRAQDYRAVYTAHTLERAVPMAFEQARAQGAQCVLFSPACASFD
CEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCHH
QFRNFEERGDRFRALIAALAS
HHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAVAVVGAGKSGQAAARWLALGGRRVVLWDGRDSEALRVVAGALAPFGVEAVLGREFVP
CEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCCCC
EEPDLSLVVVSPGVRWDHPGLVAARARGVTVTGEVGLAWESLSHRRWLCVTGTNGKTTTT
CCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCCCCCHHHCCCCEEEEEECCCCCHHHH
ALVGHILKTAGLRAPVCGNIGRPVTDLLLEPEDYDWIVAELSSFQIESAQGIRPEVAVWT
HHHHHHHHHCCCCCCCCCCCCCCHHHHEECCCCCCCCEEEHHHHEECCCCCCCCCEEEEE
TFTPDHLNRHGTLERYAAIKAGLLMQARRAVLNGDDAYLGARRSAWPDAWWTSTQAPAAV
EECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCEE
SLAGKDICIENRPVLPVSAVRLPGAHNLQNVLMAVAACHLTGVGDAAIASGVASFIGVPH
EECCCEEEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
RLEAVGEYRGVRFINDSKATNYDAALVGLTAVPAPSVLIAGGQAKTGESGPWLRAIAERC
HHHHHHHHCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHH
ASVVLIGEAAPLFEKWLRAQDYRAVYTAHTLERAVPMAFEQARAQGAQCVLFSPACASFD
CEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCHH
QFRNFEERGDRFRALIAALAS
HHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292