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The map label for this gene is fabF [C]

Identifier: 37522421

GI number: 37522421

Start: 3042296

End: 3043468

Strand: Reverse

Name: fabF [C]

Synonym: gll2852

Alternate gene names: 37522421

Gene position: 3043468-3042296 (Counterclockwise)

Preceding gene: 37522422

Following gene: 37522410

Centisome position: 65.32

GC content: 63.26

Gene sequence:

>1173_bases
ATGCCTATTGTTGGTAGTGGATATGCAGTCACGGGAATTGGCCTGGCAACCCCCATGGGTTGCGAAGCGCAAACCGTTTG
GAATCATCTGTCCCAGGGCCGGCCGCTGTTCGGCAAAGACTGTCCCGGCGCCGGTGCGGGCAGAGTTGACGACCACCATA
TCGAGCGCCGCATACCGGCCCGGCACGCACAAAAGCTCGATCGCTTCACGATGCTGGCGATGATTGCCGGCGAAAACGCT
CTGGCGGACTCCCACTTTGAGATTGCGCCGGAGAACTGCTACCGCGTCGGTCTGGTTGTGGGCAACAGCACAGGCGGATG
GACTTTCCTGGAACCGACGATGCAGGCCCTTTACAGCGAAGGCATGAAGGCGGTCAATTCTTACACCGCCACCGCCTGGT
TTCCGACCGCGGCGCAGGGAGAAATTTCGATCCACCATAGGATCGGCGGTTACAGCAAGACGGTGTGTGCCGATCGCCTC
AGTGCCGGTTTTGCCCTGGAATTGGCGAGCGTCGCCGTTGAACGCGGCCGGGTGGAAGCTGTCGTCGTCGGAGGCACGGA
GGCTCCCCTCAGCGATTTTGTGCTAAATGCCTACGCGGGTGCGGGCTGCCTCTCGCCATCGGGCTGCTACGCGCCTTTTG
AGGAAACCGCCGACGGCAGGCTGCTGGCTGAAGGTTCCGCCATGCTGGTGTTGGAGTTGAGTCCCGCCGCTGCGGCGAGG
GGGGCAAAGGTGCACTGCGAAATCCTGGCCATCGCCGGGGGGGACGATCTTGCCCAAGCGATGCGCTCGTGCCTGCACAG
CGCGGGGGTCTCCCCCGAAGCGGTGGACTATATCGTGCTCGATGCTTTCGGTACCCCGCAAAGGGATGACGAGGAATACC
GCGCCATCGGCGAAGTGTTTGCCAACCACGCCCAACTGCGTATGAGCGCCCCGAAATCCATGTACGGGGATCTCGTCGGG
GCGGGCGTGGCCATGGATGTGATCATCGCTTGCATGAGCATCGAACGGCAGGCAGTCCTGCCCACCGCCGGCACTCCTGC
GCGCGTCAAATCACCGCCCGTCGGCCGCCATGTCGTCGGCCGCCCCGAGGCGGCACCCATCCGCTACGCACTGGTAAACG
CCAGGGACGAAGCCGGCAGAAGCCTGGCCCTATTGCTCGCTAAACCGACGTGA

Upstream 100 bases:

>100_bases
GGTGCACTTTGAAGACCAGCAGCGGATTTTCCGGCATCCATTCGAGCCTGGTCATCCGCAAATTCGAGCACAGAAATTAA
ATAGATAAGAGGACAATCTT

Downstream 100 bases:

>100_bases
GCTACCTGCGCGGGCAGCGAATCCCGCCGCCTGCGAAGCCGGTCACTCGTCGTAGGTCTGGCAGGAATCCTGGTGGCAGT
CTTCCTCCCAATTGAAATGA

Product: beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: actI ORF2 [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPIVGSGYAVTGIGLATPMGCEAQTVWNHLSQGRPLFGKDCPGAGAGRVDDHHIERRIPARHAQKLDRFTMLAMIAGENA
LADSHFEIAPENCYRVGLVVGNSTGGWTFLEPTMQALYSEGMKAVNSYTATAWFPTAAQGEISIHHRIGGYSKTVCADRL
SAGFALELASVAVERGRVEAVVVGGTEAPLSDFVLNAYAGAGCLSPSGCYAPFEETADGRLLAEGSAMLVLELSPAAAAR
GAKVHCEILAIAGGDDLAQAMRSCLHSAGVSPEAVDYIVLDAFGTPQRDDEEYRAIGEVFANHAQLRMSAPKSMYGDLVG
AGVAMDVIIACMSIERQAVLPTAGTPARVKSPPVGRHVVGRPEAAPIRYALVNARDEAGRSLALLLAKPT

Sequences:

>Translated_390_residues
MPIVGSGYAVTGIGLATPMGCEAQTVWNHLSQGRPLFGKDCPGAGAGRVDDHHIERRIPARHAQKLDRFTMLAMIAGENA
LADSHFEIAPENCYRVGLVVGNSTGGWTFLEPTMQALYSEGMKAVNSYTATAWFPTAAQGEISIHHRIGGYSKTVCADRL
SAGFALELASVAVERGRVEAVVVGGTEAPLSDFVLNAYAGAGCLSPSGCYAPFEETADGRLLAEGSAMLVLELSPAAAAR
GAKVHCEILAIAGGDDLAQAMRSCLHSAGVSPEAVDYIVLDAFGTPQRDDEEYRAIGEVFANHAQLRMSAPKSMYGDLVG
AGVAMDVIIACMSIERQAVLPTAGTPARVKSPPVGRHVVGRPEAAPIRYALVNARDEAGRSLALLLAKPT
>Mature_389_residues
PIVGSGYAVTGIGLATPMGCEAQTVWNHLSQGRPLFGKDCPGAGAGRVDDHHIERRIPARHAQKLDRFTMLAMIAGENAL
ADSHFEIAPENCYRVGLVVGNSTGGWTFLEPTMQALYSEGMKAVNSYTATAWFPTAAQGEISIHHRIGGYSKTVCADRLS
AGFALELASVAVERGRVEAVVVGGTEAPLSDFVLNAYAGAGCLSPSGCYAPFEETADGRLLAEGSAMLVLELSPAAAARG
AKVHCEILAIAGGDDLAQAMRSCLHSAGVSPEAVDYIVLDAFGTPQRDDEEYRAIGEVFANHAQLRMSAPKSMYGDLVGA
GVAMDVIIACMSIERQAVLPTAGTPARVKSPPVGRHVVGRPEAAPIRYALVNARDEAGRSLALLLAKPT

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=368, Percent_Identity=26.3586956521739, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1787337, Length=410, Percent_Identity=25.8536585365854, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788663, Length=406, Percent_Identity=23.6453201970443, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI212642053, Length=326, Percent_Identity=24.8466257668712, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320904, Length=437, Percent_Identity=21.5102974828375, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24644341, Length=363, Percent_Identity=28.3746556473829, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 40901; Mature: 40770

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIVGSGYAVTGIGLATPMGCEAQTVWNHLSQGRPLFGKDCPGAGAGRVDDHHIERRIPA
CCEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCH
RHAQKLDRFTMLAMIAGENALADSHFEIAPENCYRVGLVVGNSTGGWTFLEPTMQALYSE
HHHHHHHHHHHHHHEECCCCCCCCCCEECCCCEEEEEEEEECCCCCEEEHHHHHHHHHHH
GMKAVNSYTATAWFPTAAQGEISIHHRIGGYSKTVCADRLSAGFALELASVAVERGRVEA
HHHHHHCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCEEE
VVVGGTEAPLSDFVLNAYAGAGCLSPSGCYAPFEETADGRLLAEGSAMLVLELSPAAAAR
EEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCHHHHC
GAKVHCEILAIAGGDDLAQAMRSCLHSAGVSPEAVDYIVLDAFGTPQRDDEEYRAIGEVF
CCEEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHH
ANHAQLRMSAPKSMYGDLVGAGVAMDVIIACMSIERQAVLPTAGTPARVKSPPVGRHVVG
HCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCC
RPEAAPIRYALVNARDEAGRSLALLLAKPT
CCCCCCEEEEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure 
PIVGSGYAVTGIGLATPMGCEAQTVWNHLSQGRPLFGKDCPGAGAGRVDDHHIERRIPA
CEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCH
RHAQKLDRFTMLAMIAGENALADSHFEIAPENCYRVGLVVGNSTGGWTFLEPTMQALYSE
HHHHHHHHHHHHHHEECCCCCCCCCCEECCCCEEEEEEEEECCCCCEEEHHHHHHHHHHH
GMKAVNSYTATAWFPTAAQGEISIHHRIGGYSKTVCADRLSAGFALELASVAVERGRVEA
HHHHHHCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCEEE
VVVGGTEAPLSDFVLNAYAGAGCLSPSGCYAPFEETADGRLLAEGSAMLVLELSPAAAAR
EEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCHHHHC
GAKVHCEILAIAGGDDLAQAMRSCLHSAGVSPEAVDYIVLDAFGTPQRDDEEYRAIGEVF
CCEEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHH
ANHAQLRMSAPKSMYGDLVGAGVAMDVIIACMSIERQAVLPTAGTPARVKSPPVGRHVVG
HCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCC
RPEAAPIRYALVNARDEAGRSLALLLAKPT
CCCCCCEEEEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1527048; 12000953 [H]