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The map label for this gene is fabF [C]

Identifier: 37522422

GI number: 37522422

Start: 3043489

End: 3044889

Strand: Reverse

Name: fabF [C]

Synonym: gll2853

Alternate gene names: 37522422

Gene position: 3044889-3043489 (Counterclockwise)

Preceding gene: 37522423

Following gene: 37522421

Centisome position: 65.35

GC content: 56.96

Gene sequence:

>1401_bases
GTGACACGTAATGTCCTGCCGAGTACTGAAGTGCCATGGCGTGTGGGCGCCGTCTCGATTTGGAATGCGGGCGAACACGC
CCAGAATCAAAGCAAGCTTCAACTTCTTCGATCCACAAAGGGGGAATATCTGATGTTGCCAGCTAGAAGGGTGGTTGTGA
CGGGTATGGGAACTGTGAATCCCATCGGCAAAAGTGTGAACGAGTTCTGGGAAAACTGTTGCAAGGGGGTCTCAGGTGTC
AAAGTCGTGTCGGATTTTGCGATAGCGGACAGCCAGAGCCAAATTGCGGGAGTCGTCGAGGACTTCGATTTTTGGGAATA
TTTTCCCCAGAACGATTTACTCGGAGTTGACCGCAGCTGCATTTTTGCACTGGTGGCGGCAAAGGAGGCCCTCGAATCGG
CCGGGCTTGGAGAATCCTTCCTAAAATCTTTGTCTCCGGGCAAATGCAGACTGCTGATGTCGACGGCCGCTGCCCAGATC
GCAAGCATGGAAAAAGATTTCTTGGCTTCCCTTGGCGACGAGACGCTCGCTCTGCATCGCCCCCGCGCCACCCCTGGAGT
CGTTCACTTTAATGCCACAGCAAAGACGTTGGCAAATCAGTTCGGGATTCCGGGGGAATGCCTGACCGTGACGACCGGTT
GCACAGGAGCGCTCGACGCCATGGGTTACGCCATGGACTTGATCCGGCTCGGCAAGATGGACCTGGCCGTTGTCGGCGGC
GCCGAAGCGCCGATCACGCCCATCTGTATGGCGGCTTTCAGCAAAATCGGGGCCACCACCAGCCGCAACGCCGAACCACA
GAAGGCGAGCCGTCCGTTTGACATGGGCCGGGACGGCTTTGTCCTGGCCGAGGGCAGCGGCATGTTGGTGATCGAAGCTC
TAGATCATGCCTTGCTGCGGGGAGCCCCAATCTTCGCGGAACTGGGCGGCTACGGAAGTTTGAACAACTTTTACCACATG
ACCAAAATGCCCGAAAACGGTCTGCAGCTTGCGCAGTCCGCCCGCACAGCCATAGAAGACGCTGGCATTTCTCCCGATGA
GATCGACTCAATCAATGCCCACGGTTCTTCCACGCCCCAGAACGACATTGCCGAAGCCAACGCTTACTGGCGTCTGTTTC
AAGAACGCACCCCGACGATCCCCGTCACCTGCATCAAGTCCCACATCGGTCACACATTGGCAGCGTCGAGCGCCATCGAA
GTGATCGCTTCGATTATGTCTATGCGGACTGGGATCGTTCCTCCCACCATCAACCTGGTCAAACAGGACTCGCGGTGCAT
GCTCGATGTCGTCGCCGACGAGCCGAGGCGGGCATCCGTCCGGTGCACTTTGAAGACCAGCAGCGGATTTTCCGGCATCC
ATTCGAGCCTGGTCATCCGCAAATTCGAGCACAGAAATTAA

Upstream 100 bases:

>100_bases
TGCTCAGCTAAATGGTTCGACAAAATCGCAAGCCCCGCTTGCGAAGCGCTGATCGGAGCACAGACCTTCGTTATTTTGCA
AAACAGGCAATGCTCGAACT

Downstream 100 bases:

>100_bases
ATAGATAAGAGGACAATCTTATGCCTATTGTTGGTAGTGGATATGCAGTCACGGGAATTGGCCTGGCAACCCCCATGGGT
TGCGAAGCGCAAACCGTTTG

Product: beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: actI ORF1 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTRNVLPSTEVPWRVGAVSIWNAGEHAQNQSKLQLLRSTKGEYLMLPARRVVVTGMGTVNPIGKSVNEFWENCCKGVSGV
KVVSDFAIADSQSQIAGVVEDFDFWEYFPQNDLLGVDRSCIFALVAAKEALESAGLGESFLKSLSPGKCRLLMSTAAAQI
ASMEKDFLASLGDETLALHRPRATPGVVHFNATAKTLANQFGIPGECLTVTTGCTGALDAMGYAMDLIRLGKMDLAVVGG
AEAPITPICMAAFSKIGATTSRNAEPQKASRPFDMGRDGFVLAEGSGMLVIEALDHALLRGAPIFAELGGYGSLNNFYHM
TKMPENGLQLAQSARTAIEDAGISPDEIDSINAHGSSTPQNDIAEANAYWRLFQERTPTIPVTCIKSHIGHTLAASSAIE
VIASIMSMRTGIVPPTINLVKQDSRCMLDVVADEPRRASVRCTLKTSSGFSGIHSSLVIRKFEHRN

Sequences:

>Translated_466_residues
MTRNVLPSTEVPWRVGAVSIWNAGEHAQNQSKLQLLRSTKGEYLMLPARRVVVTGMGTVNPIGKSVNEFWENCCKGVSGV
KVVSDFAIADSQSQIAGVVEDFDFWEYFPQNDLLGVDRSCIFALVAAKEALESAGLGESFLKSLSPGKCRLLMSTAAAQI
ASMEKDFLASLGDETLALHRPRATPGVVHFNATAKTLANQFGIPGECLTVTTGCTGALDAMGYAMDLIRLGKMDLAVVGG
AEAPITPICMAAFSKIGATTSRNAEPQKASRPFDMGRDGFVLAEGSGMLVIEALDHALLRGAPIFAELGGYGSLNNFYHM
TKMPENGLQLAQSARTAIEDAGISPDEIDSINAHGSSTPQNDIAEANAYWRLFQERTPTIPVTCIKSHIGHTLAASSAIE
VIASIMSMRTGIVPPTINLVKQDSRCMLDVVADEPRRASVRCTLKTSSGFSGIHSSLVIRKFEHRN
>Mature_465_residues
TRNVLPSTEVPWRVGAVSIWNAGEHAQNQSKLQLLRSTKGEYLMLPARRVVVTGMGTVNPIGKSVNEFWENCCKGVSGVK
VVSDFAIADSQSQIAGVVEDFDFWEYFPQNDLLGVDRSCIFALVAAKEALESAGLGESFLKSLSPGKCRLLMSTAAAQIA
SMEKDFLASLGDETLALHRPRATPGVVHFNATAKTLANQFGIPGECLTVTTGCTGALDAMGYAMDLIRLGKMDLAVVGGA
EAPITPICMAAFSKIGATTSRNAEPQKASRPFDMGRDGFVLAEGSGMLVIEALDHALLRGAPIFAELGGYGSLNNFYHMT
KMPENGLQLAQSARTAIEDAGISPDEIDSINAHGSSTPQNDIAEANAYWRLFQERTPTIPVTCIKSHIGHTLAASSAIEV
IASIMSMRTGIVPPTINLVKQDSRCMLDVVADEPRRASVRCTLKTSSGFSGIHSSLVIRKFEHRN

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=439, Percent_Identity=29.3849658314351, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI223972627, Length=144, Percent_Identity=32.6388888888889, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI41872631, Length=432, Percent_Identity=22.2222222222222, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1787337, Length=418, Percent_Identity=36.3636363636364, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788663, Length=428, Percent_Identity=29.9065420560748, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71983541, Length=421, Percent_Identity=29.9287410926366, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71983535, Length=259, Percent_Identity=33.976833976834, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI212642053, Length=224, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17550940, Length=222, Percent_Identity=25.2252252252252, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320904, Length=447, Percent_Identity=29.7539149888143, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24644341, Length=437, Percent_Identity=31.350114416476, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24581345, Length=228, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI221330659, Length=232, Percent_Identity=25.4310344827586, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920632, Length=232, Percent_Identity=25.4310344827586, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 49909; Mature: 49777

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNVLPSTEVPWRVGAVSIWNAGEHAQNQSKLQLLRSTKGEYLMLPARRVVVTGMGTVN
CCCCCCCCCCCCEEECEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCEEEEECCCCCC
PIGKSVNEFWENCCKGVSGVKVVSDFAIADSQSQIAGVVEDFDFWEYFPQNDLLGVDRSC
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IFALVAAKEALESAGLGESFLKSLSPGKCRLLMSTAAAQIASMEKDFLASLGDETLALHR
HHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
PRATPGVVHFNATAKTLANQFGIPGECLTVTTGCTGALDAMGYAMDLIRLGKMDLAVVGG
CCCCCCEEEECCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECC
AEAPITPICMAAFSKIGATTSRNAEPQKASRPFDMGRDGFVLAEGSGMLVIEALDHALLR
CCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHC
GAPIFAELGGYGSLNNFYHMTKMPENGLQLAQSARTAIEDAGISPDEIDSINAHGSSTPQ
CCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
NDIAEANAYWRLFQERTPTIPVTCIKSHIGHTLAASSAIEVIASIMSMRTGIVPPTINLV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KQDSRCMLDVVADEPRRASVRCTLKTSSGFSGIHSSLVIRKFEHRN
CCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRNVLPSTEVPWRVGAVSIWNAGEHAQNQSKLQLLRSTKGEYLMLPARRVVVTGMGTVN
CCCCCCCCCCCEEECEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCEEEEECCCCCC
PIGKSVNEFWENCCKGVSGVKVVSDFAIADSQSQIAGVVEDFDFWEYFPQNDLLGVDRSC
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IFALVAAKEALESAGLGESFLKSLSPGKCRLLMSTAAAQIASMEKDFLASLGDETLALHR
HHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
PRATPGVVHFNATAKTLANQFGIPGECLTVTTGCTGALDAMGYAMDLIRLGKMDLAVVGG
CCCCCCEEEECCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECC
AEAPITPICMAAFSKIGATTSRNAEPQKASRPFDMGRDGFVLAEGSGMLVIEALDHALLR
CCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHC
GAPIFAELGGYGSLNNFYHMTKMPENGLQLAQSARTAIEDAGISPDEIDSINAHGSSTPQ
CCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
NDIAEANAYWRLFQERTPTIPVTCIKSHIGHTLAASSAIEVIASIMSMRTGIVPPTINLV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KQDSRCMLDVVADEPRRASVRCTLKTSSGFSGIHSSLVIRKFEHRN
CCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953; 1527048 [H]