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The map label for this gene is cofH

Identifier: 161702943

GI number: 161702943

Start: 829477

End: 830646

Strand: Direct

Name: cofH

Synonym: glr0776

Alternate gene names: 161702943

Gene position: 829477-830646 (Clockwise)

Preceding gene: 37520342

Following gene: 37520348

Centisome position: 17.8

GC content: 67.35

Gene sequence:

>1170_bases
ATGCGCGATTGGAAACGGTTGCACGCCACCCTCGATGCTGCCCGGGAAGGCCGGGAGATGACGGTGCCCGAGGCGGCCTT
CTTGCTCGGCCAGACCGACCCGGCGGCCCGCGAGCTTGTCCGCTCGGCCGCCGACCGCCTGCGCGCCGAGCTGGTGGGTG
AGCGGGTGAGCTACGTGATCAACCGCAACATCAACTTCTCGAACATCTGTGTTCAGCACTGTTCGTTTTGCGCCTTCCGG
CGCGACGCGCAGGAAGCGGGAGCCTACAGCCTCGACTTTGCCCACATCCTGCAGAAGACTGCCGAAGCGGTCGCCTCGGG
CGCCACCGAGATCTGCATGCAGGGCGGTCTCAACCCGGCGGTGCGCGGTGCGGGCGGCCGGGTGCTCGACTATTATCTGC
AACTGGTAGATGCGATCAAAGCGCCTTTTCCCCGCATTCACCTGCACGCCTTTTCGCCGCAGGAGTCGTTTTTTATCGCC
CGCGAGGACCGGCTGCCCATCGAGACGGTGCTCGCCGAACTGCGCGCCGCCGGGGTCGATTCGATGCCCGGCACGGCGGC
GGAGGTGCTCCACGAGCCGGTGCGCCGCCGCCTCTGCCCGGAAAAGCTCAGTACCCTCGCCTGGGTGCGCACGGTCGAAG
CCGCCCACCGCACCGGTTTGCCGATGACCAGCACGATGCTGTCGGGCCACATCGAGACGGCTATGCACCGGGCCATTCAC
CTCGGCGTGCTGCGCACCATCCAGAAGCGTACCGGTGGGTTCACCGAATTTGTGCTCCTGCCCTACGTCGGACTTGCCGC
CCCCCGCGCCCTGCGCGCCCGCGTCGGGCGCGATCAGCCGGAACTGGCGGACGCGCTGCTCACCCAGGCGGTGGCGCGCC
TATTTTTTGGGCGCTCACTCGTCAATCACCAACCCAGTTGGGTGAAGCTCGGCCTGGAGGGGGCGGGCGAAGCGCTGCGC
TGGGGGTGCAACGACATCGGCGGCACCCTGATGGAGGAGCACATCACCTCGATGGCCGGAGCCCGGGGCGGCACCTGTCA
GACGCCCGAGGCTTTACGCGCAGCCGTCTTTCAGGCCGAACGCACCGCGTACCAGCGAGACACGCTCTACCAGGAAGTGG
CCGCAGAAAGTATCCAGCCGGCCGCATCGCTCAAGTCCGCCGCCAGATAA

Upstream 100 bases:

>100_bases
GTCCTTCGGGGGTTCGTCCGACGATCCCATCTTGGCAGTTTTCGCCCGGCCGCCGCTGCTCGCCCGTAACATGGTTTTGA
TCCGCCTTCGCTTGAGATCT

Downstream 100 bases:

>100_bases
TCCGGCTCGACGGTGTACTGGTAGCTGCCCGAACGCACTTGCTCCCCAAAGCCTGTCTGCACCAGAATGCCCCGGCAGCC
GGCGTTGCGGGCCAGGTCGA

Product: FO synthase subunit 2

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRDWKRLHATLDAAREGREMTVPEAAFLLGQTDPAARELVRSAADRLRAELVGERVSYVINRNINFSNICVQHCSFCAFR
RDAQEAGAYSLDFAHILQKTAEAVASGATEICMQGGLNPAVRGAGGRVLDYYLQLVDAIKAPFPRIHLHAFSPQESFFIA
REDRLPIETVLAELRAAGVDSMPGTAAEVLHEPVRRRLCPEKLSTLAWVRTVEAAHRTGLPMTSTMLSGHIETAMHRAIH
LGVLRTIQKRTGGFTEFVLLPYVGLAAPRALRARVGRDQPELADALLTQAVARLFFGRSLVNHQPSWVKLGLEGAGEALR
WGCNDIGGTLMEEHITSMAGARGGTCQTPEALRAAVFQAERTAYQRDTLYQEVAAESIQPAASLKSAAR

Sequences:

>Translated_389_residues
MRDWKRLHATLDAAREGREMTVPEAAFLLGQTDPAARELVRSAADRLRAELVGERVSYVINRNINFSNICVQHCSFCAFR
RDAQEAGAYSLDFAHILQKTAEAVASGATEICMQGGLNPAVRGAGGRVLDYYLQLVDAIKAPFPRIHLHAFSPQESFFIA
REDRLPIETVLAELRAAGVDSMPGTAAEVLHEPVRRRLCPEKLSTLAWVRTVEAAHRTGLPMTSTMLSGHIETAMHRAIH
LGVLRTIQKRTGGFTEFVLLPYVGLAAPRALRARVGRDQPELADALLTQAVARLFFGRSLVNHQPSWVKLGLEGAGEALR
WGCNDIGGTLMEEHITSMAGARGGTCQTPEALRAAVFQAERTAYQRDTLYQEVAAESIQPAASLKSAAR
>Mature_389_residues
MRDWKRLHATLDAAREGREMTVPEAAFLLGQTDPAARELVRSAADRLRAELVGERVSYVINRNINFSNICVQHCSFCAFR
RDAQEAGAYSLDFAHILQKTAEAVASGATEICMQGGLNPAVRGAGGRVLDYYLQLVDAIKAPFPRIHLHAFSPQESFFIA
REDRLPIETVLAELRAAGVDSMPGTAAEVLHEPVRRRLCPEKLSTLAWVRTVEAAHRTGLPMTSTMLSGHIETAMHRAIH
LGVLRTIQKRTGGFTEFVLLPYVGLAAPRALRARVGRDQPELADALLTQAVARLFFGRSLVNHQPSWVKLGLEGAGEALR
WGCNDIGGTLMEEHITSMAGARGGTCQTPEALRAAVFQAERTAYQRDTLYQEVAAESIQPAASLKSAAR

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO)

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFH_GLOVI (Q7NMI9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923722.2
- GeneID:   2599395
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr0776
- NMPDR:   fig|251221.1.peg.776
- HOGENOM:   HBG312134
- ProtClustDB:   PRK07360
- BioCyc:   GVIO251221:GLR0776-MONOMER
- HAMAP:   MF_01612
- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004762
- SMART:   SM00729
- TIGRFAMs:   TIGR00423
- TIGRFAMs:   TIGR03551

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 42558; Mature: 42558

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDWKRLHATLDAAREGREMTVPEAAFLLGQTDPAARELVRSAADRLRAELVGERVSYVI
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRNINFSNICVQHCSFCAFRRDAQEAGAYSLDFAHILQKTAEAVASGATEICMQGGLNPA
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCHH
VRGAGGRVLDYYLQLVDAIKAPFPRIHLHAFSPQESFFIAREDRLPIETVLAELRAAGVD
HCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCC
SMPGTAAEVLHEPVRRRLCPEKLSTLAWVRTVEAAHRTGLPMTSTMLSGHIETAMHRAIH
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LGVLRTIQKRTGGFTEFVLLPYVGLAAPRALRARVGRDQPELADALLTQAVARLFFGRSL
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VNHQPSWVKLGLEGAGEALRWGCNDIGGTLMEEHITSMAGARGGTCQTPEALRAAVFQAE
HCCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RTAYQRDTLYQEVAAESIQPAASLKSAAR
HHHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MRDWKRLHATLDAAREGREMTVPEAAFLLGQTDPAARELVRSAADRLRAELVGERVSYVI
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRNINFSNICVQHCSFCAFRRDAQEAGAYSLDFAHILQKTAEAVASGATEICMQGGLNPA
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCHH
VRGAGGRVLDYYLQLVDAIKAPFPRIHLHAFSPQESFFIAREDRLPIETVLAELRAAGVD
HCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCC
SMPGTAAEVLHEPVRRRLCPEKLSTLAWVRTVEAAHRTGLPMTSTMLSGHIETAMHRAIH
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LGVLRTIQKRTGGFTEFVLLPYVGLAAPRALRARVGRDQPELADALLTQAVARLFFGRSL
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VNHQPSWVKLGLEGAGEALRWGCNDIGGTLMEEHITSMAGARGGTCQTPEALRAAVFQAE
HCCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RTAYQRDTLYQEVAAESIQPAASLKSAAR
HHHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292