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The map label for this gene is gatA

Identifier: 34499810

GI number: 34499810

Start: 4695626

End: 4697077

Strand: Reverse

Name: gatA

Synonym: CV_4355

Alternate gene names: 34499810

Gene position: 4697077-4695626 (Counterclockwise)

Preceding gene: 34499811

Following gene: 34499809

Centisome position: 98.86

GC content: 67.08

Gene sequence:

>1452_bases
ATGACACAAGCCACCCTCAAGCAATTGTCGCAACAGCTGGCGGCCAAGCAGGTTTCCAGCGTGGAGCTGGCCAGCCAGTA
CCTGGACCGCATCGAGGCGCTGAACCCGCAGCTCAACGCCATCGTCACCGTAGACCGCGAAAAGACGCTGGCCGAGGCCC
GCGCGGCCGACGCCCGCCTCGCCGCCGGCGATGCCCGTGCCCTGACCGGCGTGCCGCTGGTGCACAAGGACCTGTTCTGC
CAGCAAGGTTGGAAAACCAGCTGCGGCTCCAGAATGCTGGACAACTTCGTTTCGCCGTACAGCGCCCACGTGGTGGAGCA
GTGCGCCGCCGCCGGCATGGTGACGCTGGGCCGCGCCAATATGGACGAGTTCGCGATGGGCTCGTCGAATGAAAACTCGT
TCTACGGCGCGGTGAAGAACCCGTGGGACCTGAACGCGATTCCGGGCGGTTCGTCCGGCGGCTCCGCCGCCGCGGTGGCC
GCGCGCCTGGCGCCGGTGGCCACCGCCACCGACACCGGCGGTTCCATCCGCCAGCCGGCCAGCCATTGCGGCGTCACCGG
CATCAAGCCGACCTACGGCGTGGTGTCGCGTTACGGCATGGTGGCCTACGCCTCCTCGCTGGACCAGGGCGGCCCGATCG
CCCAGACCGCCGAGGACTGCGCGCTGATGCTGAACGTGATGGCCGGCTTCGACGCGCGCGACTCCACCAGCCTGGAGCGC
GCCAAGGAAGACTACGCCCGCGACCTGAACCAGTCCTTGTCCGGACTGAAGGTGGGCCTGCCCAAGGAATACTTCGCCGC
CGGTCTGGACGCCGACGTCGCGCGCGCCGTCGACAACGCGGTGGCCGAGCTGAAAAAGCTGGGCGCCGAGGCGGTGGAGA
TCAGCCTGCCCAATACCGAGCTGTCGATCCCGGCTTATTACGTGATCGCCCCGGCCGAGGCCTCGACCAACCTGTCCCGC
TACGACGGCGTCCGCTACGGCCACCGCGCCAAGGACTACAAGGACTTGGTCGACATGTACGAGAAGACCCGCGCCGAAGG
CTTCGGCGACGAGGTCAAGCGCCGCATCCTGGTGGGTAGCTATGTGCTGAGCCACGGTTACTACGACGCCTACTACCTGA
AGGCGCAGAAGATCCGCCGCCTGATCGCCAACGACTTCAAGGCCGCCTTCGAGCAGTGCGACGTGATCCTGGGGCCGGTG
GCGCCGACCGCCGCCTTCAATATCGGCGAGAAGTCCGGCGATCCGGTGCAGATGTATCTGTCCGACATCTACACGCTGTC
GGTGAACCTGGCCGGCCTGCCGGGCATGAGCGTGCCGGCCGGCTTCGCCGCCAACGGCCGCCCGATCGGCCTGCAGATCA
TCGGCAACTACTTTGCCGAAGCCAGGATGCTGAACGTGGCGCACCAGTTCCAGCAGGCGACTGACTGGCACGCCAAGGCG
CCGTCGCTGTAA

Upstream 100 bases:

>100_bases
CCCAAAGTGATCGAGTAAGCCTGTGAGCCGTCCCGCCGCGATTGGTTCCCAGAAAATCGCCGGCCGGGCCAGACTATCCG
GATAGCAAGATTTGATCGAA

Downstream 100 bases:

>100_bases
AACCAGCGGCTCGACGCGAATACGGCGTTGGGCGCGCTTGCCGTACTCAATGTACTGTCTGCGCGCGCCCGCCTTGTCTT
CGCGCCGCTCGCGCGCTGGC

Product: Glu-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTQATLKQLSQQLAAKQVSSVELASQYLDRIEALNPQLNAIVTVDREKTLAEARAADARLAAGDARALTGVPLVHKDLFC
QQGWKTSCGSRMLDNFVSPYSAHVVEQCAAAGMVTLGRANMDEFAMGSSNENSFYGAVKNPWDLNAIPGGSSGGSAAAVA
ARLAPVATATDTGGSIRQPASHCGVTGIKPTYGVVSRYGMVAYASSLDQGGPIAQTAEDCALMLNVMAGFDARDSTSLER
AKEDYARDLNQSLSGLKVGLPKEYFAAGLDADVARAVDNAVAELKKLGAEAVEISLPNTELSIPAYYVIAPAEASTNLSR
YDGVRYGHRAKDYKDLVDMYEKTRAEGFGDEVKRRILVGSYVLSHGYYDAYYLKAQKIRRLIANDFKAAFEQCDVILGPV
APTAAFNIGEKSGDPVQMYLSDIYTLSVNLAGLPGMSVPAGFAANGRPIGLQIIGNYFAEARMLNVAHQFQQATDWHAKA
PSL

Sequences:

>Translated_483_residues
MTQATLKQLSQQLAAKQVSSVELASQYLDRIEALNPQLNAIVTVDREKTLAEARAADARLAAGDARALTGVPLVHKDLFC
QQGWKTSCGSRMLDNFVSPYSAHVVEQCAAAGMVTLGRANMDEFAMGSSNENSFYGAVKNPWDLNAIPGGSSGGSAAAVA
ARLAPVATATDTGGSIRQPASHCGVTGIKPTYGVVSRYGMVAYASSLDQGGPIAQTAEDCALMLNVMAGFDARDSTSLER
AKEDYARDLNQSLSGLKVGLPKEYFAAGLDADVARAVDNAVAELKKLGAEAVEISLPNTELSIPAYYVIAPAEASTNLSR
YDGVRYGHRAKDYKDLVDMYEKTRAEGFGDEVKRRILVGSYVLSHGYYDAYYLKAQKIRRLIANDFKAAFEQCDVILGPV
APTAAFNIGEKSGDPVQMYLSDIYTLSVNLAGLPGMSVPAGFAANGRPIGLQIIGNYFAEARMLNVAHQFQQATDWHAKA
PSL
>Mature_482_residues
TQATLKQLSQQLAAKQVSSVELASQYLDRIEALNPQLNAIVTVDREKTLAEARAADARLAAGDARALTGVPLVHKDLFCQ
QGWKTSCGSRMLDNFVSPYSAHVVEQCAAAGMVTLGRANMDEFAMGSSNENSFYGAVKNPWDLNAIPGGSSGGSAAAVAA
RLAPVATATDTGGSIRQPASHCGVTGIKPTYGVVSRYGMVAYASSLDQGGPIAQTAEDCALMLNVMAGFDARDSTSLERA
KEDYARDLNQSLSGLKVGLPKEYFAAGLDADVARAVDNAVAELKKLGAEAVEISLPNTELSIPAYYVIAPAEASTNLSRY
DGVRYGHRAKDYKDLVDMYEKTRAEGFGDEVKRRILVGSYVLSHGYYDAYYLKAQKIRRLIANDFKAAFEQCDVILGPVA
PTAAFNIGEKSGDPVQMYLSDIYTLSVNLAGLPGMSVPAGFAANGRPIGLQIIGNYFAEARMLNVAHQFQQATDWHAKAP
SL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=38.12375249501, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI195972892, Length=486, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17543272, Length=411, Percent_Identity=38.6861313868613, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=25.6, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=314, Percent_Identity=24.8407643312102, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=240, Percent_Identity=29.5833333333333, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=188, Percent_Identity=28.1914893617021, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=344, Percent_Identity=24.4186046511628, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=319, Percent_Identity=25.3918495297806, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=35.0467289719626, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=484, Percent_Identity=27.8925619834711, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.7959183673469, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=27.378640776699, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=26.1133603238866, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=26.1133603238866, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=26.1133603238866, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=26.1133603238866, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=27.6470588235294, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24648435, Length=498, Percent_Identity=24.6987951807229, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648437, Length=498, Percent_Identity=24.6987951807229, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550774, Length=498, Percent_Identity=24.6987951807229, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=24.5934959349593, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=431, Percent_Identity=24.1299303944316, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=431, Percent_Identity=24.1299303944316, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CHRVO (Q7NPY7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904025.1
- ProteinModelPortal:   Q7NPY7
- SMR:   Q7NPY7
- GeneID:   2549585
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4355
- NMPDR:   fig|243365.1.peg.4355
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q7NPY7
- BioCyc:   CVIO243365:CV_4355-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51550; Mature: 51419

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQATLKQLSQQLAAKQVSSVELASQYLDRIEALNPQLNAIVTVDREKTLAEARAADARL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
AAGDARALTGVPLVHKDLFCQQGWKTSCGSRMLDNFVSPYSAHVVEQCAAAGMVTLGRAN
CCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEECCCC
MDEFAMGSSNENSFYGAVKNPWDLNAIPGGSSGGSAAAVAARLAPVATATDTGGSIRQPA
CHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
SHCGVTGIKPTYGVVSRYGMVAYASSLDQGGPIAQTAEDCALMLNVMAGFDARDSTSLER
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
AKEDYARDLNQSLSGLKVGLPKEYFAAGLDADVARAVDNAVAELKKLGAEAVEISLPNTE
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
LSIPAYYVIAPAEASTNLSRYDGVRYGHRAKDYKDLVDMYEKTRAEGFGDEVKRRILVGS
CCCCEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YVLSHGYYDAYYLKAQKIRRLIANDFKAAFEQCDVILGPVAPTAAFNIGEKSGDPVQMYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHEEECCCCCCCHHHHHH
SDIYTLSVNLAGLPGMSVPAGFAANGRPIGLQIIGNYFAEARMLNVAHQFQQATDWHAKA
HHHHEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PSL
CCC
>Mature Secondary Structure 
TQATLKQLSQQLAAKQVSSVELASQYLDRIEALNPQLNAIVTVDREKTLAEARAADARL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
AAGDARALTGVPLVHKDLFCQQGWKTSCGSRMLDNFVSPYSAHVVEQCAAAGMVTLGRAN
CCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEECCCC
MDEFAMGSSNENSFYGAVKNPWDLNAIPGGSSGGSAAAVAARLAPVATATDTGGSIRQPA
CHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
SHCGVTGIKPTYGVVSRYGMVAYASSLDQGGPIAQTAEDCALMLNVMAGFDARDSTSLER
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
AKEDYARDLNQSLSGLKVGLPKEYFAAGLDADVARAVDNAVAELKKLGAEAVEISLPNTE
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
LSIPAYYVIAPAEASTNLSRYDGVRYGHRAKDYKDLVDMYEKTRAEGFGDEVKRRILVGS
CCCCEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YVLSHGYYDAYYLKAQKIRRLIANDFKAAFEQCDVILGPVAPTAAFNIGEKSGDPVQMYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHEEECCCCCCCHHHHHH
SDIYTLSVNLAGLPGMSVPAGFAANGRPIGLQIIGNYFAEARMLNVAHQFQQATDWHAKA
HHHHEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PSL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782