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The map label for this gene is gatB

Identifier: 34499809

GI number: 34499809

Start: 4694038

End: 4695465

Strand: Reverse

Name: gatB

Synonym: CV_4354

Alternate gene names: 34499809

Gene position: 4695465-4694038 (Counterclockwise)

Preceding gene: 34499810

Following gene: 34499807

Centisome position: 98.83

GC content: 64.15

Gene sequence:

>1428_bases
ATGAAATGGGAAGTCGTAATCGGTATCGAGGTGCATGTGCAGCTCAATACCGCCTCCAAGATTTTCTCCGGCGCCAGCAC
CGCTTTCGGCGCGGAGCCCAACACCCAGGCTTCCGCGGTGGAGCTGGCGCTGCCCGGCGTGTTGCCGGTGCTGAACCGCG
CGGTGGTGGACAAGGCGATCCGCCTGGGCCTGGCGCTGGATGCCAACATCAACCAGAAGAACGTGTTCGCCCGCAAGAAC
TACTTCTACCCGGACCTTCCCAAGGGCTACCAGATCAGCCAGATGGACCTGCCCATCGTCGAGCACGGCAAGCTGAAAAT
CCTGGTTGGCGATCAGGAAAAAGTCATCGGCGTGACGCGCGCCCACATGGAAGAGGACGCCGGCAAGAGCCTGCACGAAG
ACTTCCAGGGCCTGTCCGGCATCGACCTGAACCGCGCCGGCACGCCGCTGCTGGAAGTGGTGTCCGAGCCCGACATGCGC
TCGGTCGACGAGGCGCTGGCCTACGTCAAGGCCTTGTACACCCTGGTGACCTGGCTGGGCATCTGTGACGGCAATATGCA
GGAAGGCAGCTTCCGCATGGACGTCAACGTGTCGGTGCGCCCGGAAGGCCAAAAGGAATTCGGCACCCGCCGCGAGATCA
AGAACCTCAACTCCTTCCGCTTCCTGGAACAGGCCGCCAAGTACGAGATCCAGTGGCAGATCGACACGCTGGAAGACGGC
GGCAAGGTGCAGCAGGCCACCGTGCTGTTCGACCCGGATACCGGTGAGACCCGCATGATGCGCAGCAAGGAAGACGCGCA
CGACTATCGCTACTTCCCGGACCCGGACTTGCTGCCGGTGCGCATCTCCGACGAGCAGGTTGCCCGCATCAAGGGCGAGA
TGCCGGAGCTGCCGGGCGCGATGCAGGCCCGCTTCGTCGACGCTTACGGCGTGTCCGCCTACGATGCGGCGCTGCTCACC
TCCAGCCTGGCGCAGGCGCAGTATTTCGAAGCCGTGGCCAAGGCCTCCGGCCAGGGCAAGCTGGCGGCCAACTGGATCAA
TGGCGAGATCGCCGCGCGCCTGAACCGCGACGGCAAGGACATCCTAGACTGCCCGATCTCGGCTGAGCGCCTGTCCGGCC
TGATCGCGCGCATCGCCGACAACACCCTGTCCAGCAAGCTGGCCAAGCAGGTGTTCGACGCGCTGTGGGACAGCGAGCTG
TCCGCCGACGCCATCATCGAGCGCGACGGTCTGAAGCAGGTGTCGGACGTCGGCGCCATCGAGAAAATGGTGGAAGAGGC
GATCGCCGCCAATCCGAAGGCGGTGGAGGAATTCCGCGCCGGCAAGGAAAAGGCGCTCAACGCCTTGGCCGGCCAGGTGA
TGAAGGCGTCCAAGGGCAAGGCCAATCCGGCCCAGGTGCAGGATATCCTGCGGCAAAAACTGTCCTGA

Upstream 100 bases:

>100_bases
GCGCGCGCCCGCCTTGTCTTCGCGCCGCTCGCGCGCTGGCTAATAAGTTTACGTTTCCCAGAGCGATGCTGCGCTCTGGC
ATGAAAAGGATTTTCCGACT

Downstream 100 bases:

>100_bases
GCGCTCTGCGTTTGAAACAGCAAGGCGGCCTGAATGGCCGCCCTGTTGTTTTTTAAATACATGGCTGAAAATTGCCGCCG
GTAATTTGTTGCTTTTCCTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKWEVVIGIEVHVQLNTASKIFSGASTAFGAEPNTQASAVELALPGVLPVLNRAVVDKAIRLGLALDANINQKNVFARKN
YFYPDLPKGYQISQMDLPIVEHGKLKILVGDQEKVIGVTRAHMEEDAGKSLHEDFQGLSGIDLNRAGTPLLEVVSEPDMR
SVDEALAYVKALYTLVTWLGICDGNMQEGSFRMDVNVSVRPEGQKEFGTRREIKNLNSFRFLEQAAKYEIQWQIDTLEDG
GKVQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPVRISDEQVARIKGEMPELPGAMQARFVDAYGVSAYDAALLT
SSLAQAQYFEAVAKASGQGKLAANWINGEIAARLNRDGKDILDCPISAERLSGLIARIADNTLSSKLAKQVFDALWDSEL
SADAIIERDGLKQVSDVGAIEKMVEEAIAANPKAVEEFRAGKEKALNALAGQVMKASKGKANPAQVQDILRQKLS

Sequences:

>Translated_475_residues
MKWEVVIGIEVHVQLNTASKIFSGASTAFGAEPNTQASAVELALPGVLPVLNRAVVDKAIRLGLALDANINQKNVFARKN
YFYPDLPKGYQISQMDLPIVEHGKLKILVGDQEKVIGVTRAHMEEDAGKSLHEDFQGLSGIDLNRAGTPLLEVVSEPDMR
SVDEALAYVKALYTLVTWLGICDGNMQEGSFRMDVNVSVRPEGQKEFGTRREIKNLNSFRFLEQAAKYEIQWQIDTLEDG
GKVQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPVRISDEQVARIKGEMPELPGAMQARFVDAYGVSAYDAALLT
SSLAQAQYFEAVAKASGQGKLAANWINGEIAARLNRDGKDILDCPISAERLSGLIARIADNTLSSKLAKQVFDALWDSEL
SADAIIERDGLKQVSDVGAIEKMVEEAIAANPKAVEEFRAGKEKALNALAGQVMKASKGKANPAQVQDILRQKLS
>Mature_475_residues
MKWEVVIGIEVHVQLNTASKIFSGASTAFGAEPNTQASAVELALPGVLPVLNRAVVDKAIRLGLALDANINQKNVFARKN
YFYPDLPKGYQISQMDLPIVEHGKLKILVGDQEKVIGVTRAHMEEDAGKSLHEDFQGLSGIDLNRAGTPLLEVVSEPDMR
SVDEALAYVKALYTLVTWLGICDGNMQEGSFRMDVNVSVRPEGQKEFGTRREIKNLNSFRFLEQAAKYEIQWQIDTLEDG
GKVQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPVRISDEQVARIKGEMPELPGAMQARFVDAYGVSAYDAALLT
SSLAQAQYFEAVAKASGQGKLAANWINGEIAARLNRDGKDILDCPISAERLSGLIARIADNTLSSKLAKQVFDALWDSEL
SADAIIERDGLKQVSDVGAIEKMVEEAIAANPKAVEEFRAGKEKALNALAGQVMKASKGKANPAQVQDILRQKLS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=37.7016129032258, Blast_Score=339, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=37.953795379538, Blast_Score=210, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=31.1023622047244, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45550808, Length=484, Percent_Identity=35.7438016528926, Blast_Score=314, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CHRVO (Q7NPY8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904024.1
- ProteinModelPortal:   Q7NPY8
- SMR:   Q7NPY8
- GeneID:   2549584
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4354
- NMPDR:   fig|243365.1.peg.4354
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q7NPY8
- ProtClustDB:   PRK05477
- BioCyc:   CVIO243365:CV_4354-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52051; Mature: 52051

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWEVVIGIEVHVQLNTASKIFSGASTAFGAEPNTQASAVELALPGVLPVLNRAVVDKAI
CCEEEEEEEEEEEEECCHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
RLGLALDANINQKNVFARKNYFYPDLPKGYQISQMDLPIVEHGKLKILVGDQEKVIGVTR
HHCEEEECCCCCCCEEEECCCCCCCCCCCCEECCCCCCEECCCEEEEEECCCHHHHHHHH
AHMEEDAGKSLHEDFQGLSGIDLNRAGTPLLEVVSEPDMRSVDEALAYVKALYTLVTWLG
HHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMQEGSFRMDVNVSVRPEGQKEFGTRREIKNLNSFRFLEQAAKYEIQWQIDTLEDG
HCCCCCCCCCEEEEEEEEECCCCHHHHCHHHHHHCCHHHHHHHHHHCEEEEEEEEECCCC
GKVQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPVRISDEQVARIKGEMPELPGA
CCEEEEEEEECCCCCHHHHHHCCCHHHCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCH
MQARFVDAYGVSAYDAALLTSSLAQAQYFEAVAKASGQGKLAANWINGEIAARLNRDGKD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCH
ILDCPISAERLSGLIARIADNTLSSKLAKQVFDALWDSELSADAIIERDGLKQVSDVGAI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHH
EKMVEEAIAANPKAVEEFRAGKEKALNALAGQVMKASKGKANPAQVQDILRQKLS
HHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKWEVVIGIEVHVQLNTASKIFSGASTAFGAEPNTQASAVELALPGVLPVLNRAVVDKAI
CCEEEEEEEEEEEEECCHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
RLGLALDANINQKNVFARKNYFYPDLPKGYQISQMDLPIVEHGKLKILVGDQEKVIGVTR
HHCEEEECCCCCCCEEEECCCCCCCCCCCCEECCCCCCEECCCEEEEEECCCHHHHHHHH
AHMEEDAGKSLHEDFQGLSGIDLNRAGTPLLEVVSEPDMRSVDEALAYVKALYTLVTWLG
HHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMQEGSFRMDVNVSVRPEGQKEFGTRREIKNLNSFRFLEQAAKYEIQWQIDTLEDG
HCCCCCCCCCEEEEEEEEECCCCHHHHCHHHHHHCCHHHHHHHHHHCEEEEEEEEECCCC
GKVQQATVLFDPDTGETRMMRSKEDAHDYRYFPDPDLLPVRISDEQVARIKGEMPELPGA
CCEEEEEEEECCCCCHHHHHHCCCHHHCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCH
MQARFVDAYGVSAYDAALLTSSLAQAQYFEAVAKASGQGKLAANWINGEIAARLNRDGKD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCH
ILDCPISAERLSGLIARIADNTLSSKLAKQVFDALWDSELSADAIIERDGLKQVSDVGAI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHH
EKMVEEAIAANPKAVEEFRAGKEKALNALAGQVMKASKGKANPAQVQDILRQKLS
HHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782