| Definition | Chromobacterium violaceum ATCC 12472 chromosome, complete genome. |
|---|---|
| Accession | NC_005085 |
| Length | 4,751,080 |
Click here to switch to the map view.
The map label for this gene is murF [H]
Identifier: 34499802
GI number: 34499802
Start: 4687368
End: 4688729
Strand: Reverse
Name: murF [H]
Synonym: CV_4347
Alternate gene names: 34499802
Gene position: 4688729-4687368 (Counterclockwise)
Preceding gene: 34499803
Following gene: 34499801
Centisome position: 98.69
GC content: 69.53
Gene sequence:
>1362_bases ATGATGACGCTGAGCCAAGCCGCCCGTTTCGCGCAAGGCCGCCTCGTCGGCGCCGACGGCGAAGTGTCGCGCGTGATCAC CGACAGCCGCCTGGCCCAGCCCGGCGACTTGTTCGTCGCGCTGAAGGGCGAACGTTTCGACGCCCACGATTTCGTCGCCG AGGTTGTCGCCCGCGGCGCCGCCGCCTTGGTGCGCGACGGCTTCGAGCTGGCCGGCGCCAGCCTGATCCAGGCCGGCGAC GACACCCGGCTGGCGCTGGGTCGTCTGGCCGCCGGCTGGCGCGAGACGCAGCCGGCCATCCGTATCGGCGTCACCGGCTC CAACGGCAAGACCACGGTCAAGGAAATGCTGGCGGCGATACTGCGCGCCCATGCCGGAGCGGATGCGGTGCTGGCCACCG CCGGCAATTTCAATAACGACATCGGCCTGCCGCTGACCTTGCTGCAGCTGAAGCCGCAGCATCGCTACGCGGTGATCGAG ATGGGCATGAACCATCACGGCGAGCTCAGCTACCTGACCGGGCTGACCCGGCCGCAGGTGGCGCTGGTCAACAACGCGAT GCGCGCCCATTTCGGGCACTTCGGCTCCACCGAGGACGTCGCCCGCGCCAAGGCCGAGATTTTCGAAGGCCTGATCGACG CCGGCGTGGCCGTGGTCAACGCCGACGACGCCAATCTGCCGCTGTTCCGCGCCGCCGCCGGCTCGCACCGCCAGCTGAGC TTCGGCCTGGGGCTGGCGGACGTCAGCGCGCGCGATCTGGCGCTGAGCGCGCTGGACAGCCGCTTCACGCTGGTCTCTCC GGCCGGACAGCTTGCCGTCTCGCTGCCGGCGCCGGGCGAGCACAACGTACGCAACGCGCTGGCCGCGGCGGCCGCCGCGC TGGCGCTGGAGGTGCCGCTGGCCGCCATCGCAGAGGGGCTGTCCGCCTTCGCCGGCGCCAAGGGCAGGCTGCAGATCAAG CAGAGCCCGCGCGGCCTGACCGTGATCGACGACAGCTACAACGCCAACCCGGATTCGATGAAGGCCGGCATCGACGTGCT GGCCGGCCTGCCCGGTCCGCGCTGTTTCGTGATGGGCGACATCGGCGAGCTGGGCGAGGCCGCGCCAGCCTTGCATGCCG AAGTCGGGGAACACGCGCGCCTGCGCGGCATCGAACAGCTCTTTGCGCTGGGCGAGGCCTCGCGCCGCGCGGTTCAGGCC TTCGGCGGCGCCGGGCAGGGTTTCGATTCGATTGACGAGCTGTTGGAGTGCCTGGATAGCCGGCTGGCTTCGGGTACCGC CGTTCTGGTCAAGGGGTCGCGCTTTATGCGCATGGAACGGGTGGTGGAACACCTGATGCAAGCAAAGAAAGAGGGAGTTT AA
Upstream 100 bases:
>100_bases GCAAGGGCCACGAGGAATACCAGGACATCGCCGGCGTCAAACGGCCGTTCTCCGACTTCCGCGTGGCGGAAGAAGCGCTG ACCGCGTGGGGGAAACGGCC
Downstream 100 bases:
>100_bases GGTGCTGCTTTGGCTGGCTGATCTGTTGGGTTCGCATATCCGGGCCTTCAATGTCTTCAATTACACCACGCTGCGCGCGG TGATGGCCGCGTTGACCGCG
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl- D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 453; Mature: 453
Protein sequence:
>453_residues MMTLSQAARFAQGRLVGADGEVSRVITDSRLAQPGDLFVALKGERFDAHDFVAEVVARGAAALVRDGFELAGASLIQAGD DTRLALGRLAAGWRETQPAIRIGVTGSNGKTTVKEMLAAILRAHAGADAVLATAGNFNNDIGLPLTLLQLKPQHRYAVIE MGMNHHGELSYLTGLTRPQVALVNNAMRAHFGHFGSTEDVARAKAEIFEGLIDAGVAVVNADDANLPLFRAAAGSHRQLS FGLGLADVSARDLALSALDSRFTLVSPAGQLAVSLPAPGEHNVRNALAAAAAALALEVPLAAIAEGLSAFAGAKGRLQIK QSPRGLTVIDDSYNANPDSMKAGIDVLAGLPGPRCFVMGDIGELGEAAPALHAEVGEHARLRGIEQLFALGEASRRAVQA FGGAGQGFDSIDELLECLDSRLASGTAVLVKGSRFMRMERVVEHLMQAKKEGV
Sequences:
>Translated_453_residues MMTLSQAARFAQGRLVGADGEVSRVITDSRLAQPGDLFVALKGERFDAHDFVAEVVARGAAALVRDGFELAGASLIQAGD DTRLALGRLAAGWRETQPAIRIGVTGSNGKTTVKEMLAAILRAHAGADAVLATAGNFNNDIGLPLTLLQLKPQHRYAVIE MGMNHHGELSYLTGLTRPQVALVNNAMRAHFGHFGSTEDVARAKAEIFEGLIDAGVAVVNADDANLPLFRAAAGSHRQLS FGLGLADVSARDLALSALDSRFTLVSPAGQLAVSLPAPGEHNVRNALAAAAAALALEVPLAAIAEGLSAFAGAKGRLQIK QSPRGLTVIDDSYNANPDSMKAGIDVLAGLPGPRCFVMGDIGELGEAAPALHAEVGEHARLRGIEQLFALGEASRRAVQA FGGAGQGFDSIDELLECLDSRLASGTAVLVKGSRFMRMERVVEHLMQAKKEGV >Mature_453_residues MMTLSQAARFAQGRLVGADGEVSRVITDSRLAQPGDLFVALKGERFDAHDFVAEVVARGAAALVRDGFELAGASLIQAGD DTRLALGRLAAGWRETQPAIRIGVTGSNGKTTVKEMLAAILRAHAGADAVLATAGNFNNDIGLPLTLLQLKPQHRYAVIE MGMNHHGELSYLTGLTRPQVALVNNAMRAHFGHFGSTEDVARAKAEIFEGLIDAGVAVVNADDANLPLFRAAAGSHRQLS FGLGLADVSARDLALSALDSRFTLVSPAGQLAVSLPAPGEHNVRNALAAAAAALALEVPLAAIAEGLSAFAGAKGRLQIK QSPRGLTVIDDSYNANPDSMKAGIDVLAGLPGPRCFVMGDIGELGEAAPALHAEVGEHARLRGIEQLFALGEASRRAVQA FGGAGQGFDSIDELLECLDSRLASGTAVLVKGSRFMRMERVVEHLMQAKKEGV
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=451, Percent_Identity=44.7893569844789, Blast_Score=310, Evalue=1e-85, Organism=Escherichia coli, GI1786276, Length=317, Percent_Identity=29.6529968454259, Blast_Score=65, Evalue=1e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 47307; Mature: 47307
Theoretical pI: Translated: 6.38; Mature: 6.38
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMTLSQAARFAQGRLVGADGEVSRVITDSRLAQPGDLFVALKGERFDAHDFVAEVVARGA CCCHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCH AALVRDGFELAGASLIQAGDDTRLALGRLAAGWRETQPAIRIGVTGSNGKTTVKEMLAAI HHHHHCCHHHHCCHHEECCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHH LRAHAGADAVLATAGNFNNDIGLPLTLLQLKPQHRYAVIEMGMNHHGELSYLTGLTRPQV HHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHCCCCCHH ALVNNAMRAHFGHFGSTEDVARAKAEIFEGLIDAGVAVVNADDANLPLFRAAAGSHRQLS HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEE FGLGLADVSARDLALSALDSRFTLVSPAGQLAVSLPAPGEHNVRNALAAAAAALALEVPL ECCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCH AAIAEGLSAFAGAKGRLQIKQSPRGLTVIDDSYNANPDSMKAGIDVLAGLPGPRCFVMGD HHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC IGELGEAAPALHAEVGEHARLRGIEQLFALGEASRRAVQAFGGAGQGFDSIDELLECLDS CHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH RLASGTAVLVKGSRFMRMERVVEHLMQAKKEGV HHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MMTLSQAARFAQGRLVGADGEVSRVITDSRLAQPGDLFVALKGERFDAHDFVAEVVARGA CCCHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCH AALVRDGFELAGASLIQAGDDTRLALGRLAAGWRETQPAIRIGVTGSNGKTTVKEMLAAI HHHHHCCHHHHCCHHEECCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHH LRAHAGADAVLATAGNFNNDIGLPLTLLQLKPQHRYAVIEMGMNHHGELSYLTGLTRPQV HHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHCCCCCHH ALVNNAMRAHFGHFGSTEDVARAKAEIFEGLIDAGVAVVNADDANLPLFRAAAGSHRQLS HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEE FGLGLADVSARDLALSALDSRFTLVSPAGQLAVSLPAPGEHNVRNALAAAAAALALEVPL ECCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCH AAIAEGLSAFAGAKGRLQIKQSPRGLTVIDDSYNANPDSMKAGIDVLAGLPGPRCFVMGD HHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC IGELGEAAPALHAEVGEHARLRGIEQLFALGEASRRAVQAFGGAGQGFDSIDELLECLDS CHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH RLASGTAVLVKGSRFMRMERVVEHLMQAKKEGV HHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]