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The map label for this gene is murE

Identifier: 34499803

GI number: 34499803

Start: 4688726

End: 4690207

Strand: Reverse

Name: murE

Synonym: CV_4348

Alternate gene names: 34499803

Gene position: 4690207-4688726 (Counterclockwise)

Preceding gene: 34499804

Following gene: 34499802

Centisome position: 98.72

GC content: 70.31

Gene sequence:

>1482_bases
ATGAAGAGCCGTTTGACTGCGTTGCCGGACTGGGATCCGGCCTTGTTGCAACAGCTGGGCCTCCCGATCAAGCGGGTGGA
GGCCGACAGCCGCCGCGTGCTGCCGGGCGATGTGTTCCTCGCCTGCCGCGGCGAGTACGCCGACGGCCGCGACTTCATCC
CCGCCGCGCTGGAAAAGGGCGCGGCCGCGGTGCTGTGGGACGAAGCCGACGGCTTCGCCTGGAAAGCCGAGTGGCAAGCG
CCCAATCTGGCGGTGCCGAATCTGCGCGAGCGCGCCGGCATCGTCGCCGCCCACGTGCTGGGCCTGCCCTCGCGCGACCT
GACCGTGGTCGGCATCACCGGCACCAACGGCAAGACCTCCATTTCGCACTGGCTGGCGCAGGCCTTCTCGCTGCTGGGCC
AGAAAGCGGCGCTGATCGGCACCGTCGGCAACGGCTTCTACGGCCACTTGACCGAAACCACCCATACCACGCCGGACCCG
GTGACGGTGCAGCAGAAGCTGGCCGAATACCGCCGCCAGGGCGCGCACGTGGTGACGATGGAAGTGTCCAGCCACGGCCT
GGACCAGTTCCGCGTCAACGGCGTCGAGTTCGCCACCGCGGTGTTCACCAACCTGACCCGCGACCACCTCGACTACCACG
GCTCGATGGAAGCCTATGGCGAGTCCAAGAAGAAGCTGTTCTTCTGGGAAGGCCTGAAGCACGCGGTGATCAACGCCGAC
GACGCCTTCGGCCGCCAGCTCGCCGCCGGGATCGACCCCAAGCAGACCCGCGTGGTCACCTATGGCCTGGAGCAGGGCGA
CGTGCGTCCGCTGGCGCTGGCCGCCACGCTGGAAGGCCTGCAGCTGACCGTGGCCACGCCGTGGGGCACGGTCGACGTGC
GCACCGGCCTGGTCGGCCGCTTCAACGCCGCCAACCTGCTGGCCTGTCTGGCCACGCTGTGCGTCAACGGCGTCAGCCTG
CAGGACGCCGCCGCGGTGATGGCGCGCATCCAGCCGGCGCGCGGCCGGATGCAGAGCGTCGGCGGCGCGCACGAGCCGCT
GGTGGTGATCGATTACGCCCACACGCCCGACGCGCTGGAGAAGGCGCTGGCCACGCTGTCCGAGATCCGCCCCGCCGGCG
GCAGGCTGTTCTGCGTGTTCGGCTGCGGCGGCGACCGCGATCCGGGCAAACGTCCGATGATGGGCGCCATCGCCGAAAAG
CACGCCGACGTGGCGGTGCTGACCAGCGACAACCCGCGCAGCGAGGACCCGCAGGCCATCATCCGCGACGTGCTGGCCGG
CATGGACGCCGCCCGCGCGCATGTCGAGGCCGACCGCGAGGCCGCCATCCACTGGGCGGTGGCCCAGGCCCGCGTCGGCG
ACGTGGTGCTGGTGGCCGGCAAGGGCCACGAGGAATACCAGGACATCGCCGGCGTCAAACGGCCGTTCTCCGACTTCCGC
GTGGCGGAAGAAGCGCTGACCGCGTGGGGGAAACGGCCATGA

Upstream 100 bases:

>100_bases
GCGGGCGGCGCGCTGCGCGTGCTGAACATCGCGCCGGACGAGCCATCCAACAACACTTTATTGCCGGAATCGACCCCGGT
TCCGGCGGATTTCTAAGATC

Downstream 100 bases:

>100_bases
TGACGCTGAGCCAAGCCGCCCGTTTCGCGCAAGGCCGCCTCGTCGGCGCCGACGGCGAAGTGTCGCGCGTGATCACCGAC
AGCCGCCTGGCCCAGCCCGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKSRLTALPDWDPALLQQLGLPIKRVEADSRRVLPGDVFLACRGEYADGRDFIPAALEKGAAAVLWDEADGFAWKAEWQA
PNLAVPNLRERAGIVAAHVLGLPSRDLTVVGITGTNGKTSISHWLAQAFSLLGQKAALIGTVGNGFYGHLTETTHTTPDP
VTVQQKLAEYRRQGAHVVTMEVSSHGLDQFRVNGVEFATAVFTNLTRDHLDYHGSMEAYGESKKKLFFWEGLKHAVINAD
DAFGRQLAAGIDPKQTRVVTYGLEQGDVRPLALAATLEGLQLTVATPWGTVDVRTGLVGRFNAANLLACLATLCVNGVSL
QDAAAVMARIQPARGRMQSVGGAHEPLVVIDYAHTPDALEKALATLSEIRPAGGRLFCVFGCGGDRDPGKRPMMGAIAEK
HADVAVLTSDNPRSEDPQAIIRDVLAGMDAARAHVEADREAAIHWAVAQARVGDVVLVAGKGHEEYQDIAGVKRPFSDFR
VAEEALTAWGKRP

Sequences:

>Translated_493_residues
MKSRLTALPDWDPALLQQLGLPIKRVEADSRRVLPGDVFLACRGEYADGRDFIPAALEKGAAAVLWDEADGFAWKAEWQA
PNLAVPNLRERAGIVAAHVLGLPSRDLTVVGITGTNGKTSISHWLAQAFSLLGQKAALIGTVGNGFYGHLTETTHTTPDP
VTVQQKLAEYRRQGAHVVTMEVSSHGLDQFRVNGVEFATAVFTNLTRDHLDYHGSMEAYGESKKKLFFWEGLKHAVINAD
DAFGRQLAAGIDPKQTRVVTYGLEQGDVRPLALAATLEGLQLTVATPWGTVDVRTGLVGRFNAANLLACLATLCVNGVSL
QDAAAVMARIQPARGRMQSVGGAHEPLVVIDYAHTPDALEKALATLSEIRPAGGRLFCVFGCGGDRDPGKRPMMGAIAEK
HADVAVLTSDNPRSEDPQAIIRDVLAGMDAARAHVEADREAAIHWAVAQARVGDVVLVAGKGHEEYQDIAGVKRPFSDFR
VAEEALTAWGKRP
>Mature_493_residues
MKSRLTALPDWDPALLQQLGLPIKRVEADSRRVLPGDVFLACRGEYADGRDFIPAALEKGAAAVLWDEADGFAWKAEWQA
PNLAVPNLRERAGIVAAHVLGLPSRDLTVVGITGTNGKTSISHWLAQAFSLLGQKAALIGTVGNGFYGHLTETTHTTPDP
VTVQQKLAEYRRQGAHVVTMEVSSHGLDQFRVNGVEFATAVFTNLTRDHLDYHGSMEAYGESKKKLFFWEGLKHAVINAD
DAFGRQLAAGIDPKQTRVVTYGLEQGDVRPLALAATLEGLQLTVATPWGTVDVRTGLVGRFNAANLLACLATLCVNGVSL
QDAAAVMARIQPARGRMQSVGGAHEPLVVIDYAHTPDALEKALATLSEIRPAGGRLFCVFGCGGDRDPGKRPMMGAIAEK
HADVAVLTSDNPRSEDPQAIIRDVLAGMDAARAHVEADREAAIHWAVAQARVGDVVLVAGKGHEEYQDIAGVKRPFSDFR
VAEEALTAWGKRP

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=465, Percent_Identity=46.2365591397849, Blast_Score=331, Evalue=6e-92,
Organism=Escherichia coli, GI1786274, Length=372, Percent_Identity=27.4193548387097, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CHRVO (Q7NPZ4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904018.1
- ProteinModelPortal:   Q7NPZ4
- SMR:   Q7NPZ4
- GeneID:   2549599
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4348
- NMPDR:   fig|243365.1.peg.4348
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- PhylomeDB:   Q7NPZ4
- ProtClustDB:   PRK00139
- BioCyc:   CVIO243365:CV_4348-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 52958; Mature: 52958

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 183-183 BINDING 189-189 BINDING 191-191 BINDING 386-386 BINDING 460-460 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRLTALPDWDPALLQQLGLPIKRVEADSRRVLPGDVFLACRGEYADGRDFIPAALEKG
CCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCC
AAAVLWDEADGFAWKAEWQAPNLAVPNLRERAGIVAAHVLGLPSRDLTVVGITGTNGKTS
CEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
ISHWLAQAFSLLGQKAALIGTVGNGFYGHLTETTHTTPDPVTVQQKLAEYRRQGAHVVTM
HHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
EVSSHGLDQFRVNGVEFATAVFTNLTRDHLDYHGSMEAYGESKKKLFFWEGLKHAVINAD
EECCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCHHEECCC
DAFGRQLAAGIDPKQTRVVTYGLEQGDVRPLALAATLEGLQLTVATPWGTVDVRTGLVGR
HHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCEEEEECCCCCEEECCCCCCC
FNAANLLACLATLCVNGVSLQDAAAVMARIQPARGRMQSVGGAHEPLVVIDYAHTPDALE
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCHHHHH
KALATLSEIRPAGGRLFCVFGCGGDRDPGKRPMMGAIAEKHADVAVLTSDNPRSEDPQAI
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHH
IRDVLAGMDAARAHVEADREAAIHWAVAQARVGDVVLVAGKGHEEYQDIAGVKRPFSDFR
HHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCHHHHH
VAEEALTAWGKRP
HHHHHHHHCCCCC
>Mature Secondary Structure
MKSRLTALPDWDPALLQQLGLPIKRVEADSRRVLPGDVFLACRGEYADGRDFIPAALEKG
CCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCC
AAAVLWDEADGFAWKAEWQAPNLAVPNLRERAGIVAAHVLGLPSRDLTVVGITGTNGKTS
CEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
ISHWLAQAFSLLGQKAALIGTVGNGFYGHLTETTHTTPDPVTVQQKLAEYRRQGAHVVTM
HHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
EVSSHGLDQFRVNGVEFATAVFTNLTRDHLDYHGSMEAYGESKKKLFFWEGLKHAVINAD
EECCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCHHEECCC
DAFGRQLAAGIDPKQTRVVTYGLEQGDVRPLALAATLEGLQLTVATPWGTVDVRTGLVGR
HHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCEEEEECCCCCEEECCCCCCC
FNAANLLACLATLCVNGVSLQDAAAVMARIQPARGRMQSVGGAHEPLVVIDYAHTPDALE
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCHHHHH
KALATLSEIRPAGGRLFCVFGCGGDRDPGKRPMMGAIAEKHADVAVLTSDNPRSEDPQAI
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHH
IRDVLAGMDAARAHVEADREAAIHWAVAQARVGDVVLVAGKGHEEYQDIAGVKRPFSDFR
HHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCHHHHH
VAEEALTAWGKRP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782