| Definition | Chromobacterium violaceum ATCC 12472 chromosome, complete genome. |
|---|---|
| Accession | NC_005085 |
| Length | 4,751,080 |
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The map label for this gene is gra-orf6 [H]
Identifier: 34499587
GI number: 34499587
Start: 4472107
End: 4472865
Strand: Reverse
Name: gra-orf6 [H]
Synonym: CV_4132
Alternate gene names: 34499587
Gene position: 4472865-4472107 (Counterclockwise)
Preceding gene: 34499591
Following gene: 34499586
Centisome position: 94.14
GC content: 71.81
Gene sequence:
>759_bases ATGCTAACAGAGCAACAACAAGATGAGCGCGCGGTCCTGATCACCGGCGCCGCGCGGCGGGTGGGCGCGGGCATCGCCCG GCAGCTGCACGCGCGCGGGCTGAGGCTGGTTTTGCACTACCGGGGCTCGCGCGGCGAAGCCGAGGCGCTGGCGGCGGAGT TGAACCGCGCGCGGCCGGACAGCGTCAGGCTGGTCCAGGCCGATCTGCTGGACGCCGCGTCGCTGCCGGAGCTGGCGGCG CGGGCGGTGGCGGCCTTCGGCCGGCTGGACGGCCTGGTCAACAACGCGTCCAGCTTCTTTCCGACCGAGGTCGGAAAGAT AGACGAGGCCGCCTGGCACGACCTGATGGGCAGCAATCTGAAGGCGCCGCTGTTCCTCAGCCAGGCGCTGGCGCCGGAGC TGACGCGCCGCGGCGGCGCCATCGTCGGCATCGCCGACATCCATGTGGACAGGCCGATGGCGCGCCACGTGGTGTACAAC CTGGCCAAGGCCGGCCACGCCCAGCTGATCCGCAGCCTGGCGATCGAGCTGGCGCCGGCGGTGCGCGTCAACGGCGTGGC GCCGGGCGTCAATCTGTGGCCGGAGAGCGAGGCTTCGTTCGACGCCGCCGGGCGCGCGGCGATCGAGGCGACCATTCCGC TGCGGCGCACCGGCGTGCCGGAAGACATCGCCCGTGCGGTGGCTTTCCTGCTGCTGGACGCTTCCTACGTCACCGGCCAG ATCCTGGCGGTGGACGGCGGCCGCAGCATCGTGTTGTAG
Upstream 100 bases:
>100_bases GGGTAGGGTGGTCATGGCTGTCGTCCTGCTACAATGTGGCGCGATTGTGGTGCAAAGCGTCGCGCCCGTGAAGGGGCGGG ACATTCGATTCGAGGAAGCA
Downstream 100 bases:
>100_bases GCGCTTTCCGCCGCCATGGCTTTTGGCGGCGGGAGGGGGTAAAATCGCCTGTTTTTCAATTTCTTAGCCGTACCCATGTC CGAACTGAACGCCGCGCTCG
Product: pteridine reductase
Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]
Alternate protein names: ORF6 [H]
Number of amino acids: Translated: 252; Mature: 252
Protein sequence:
>252_residues MLTEQQQDERAVLITGAARRVGAGIARQLHARGLRLVLHYRGSRGEAEALAAELNRARPDSVRLVQADLLDAASLPELAA RAVAAFGRLDGLVNNASSFFPTEVGKIDEAAWHDLMGSNLKAPLFLSQALAPELTRRGGAIVGIADIHVDRPMARHVVYN LAKAGHAQLIRSLAIELAPAVRVNGVAPGVNLWPESEASFDAAGRAAIEATIPLRRTGVPEDIARAVAFLLLDASYVTGQ ILAVDGGRSIVL
Sequences:
>Translated_252_residues MLTEQQQDERAVLITGAARRVGAGIARQLHARGLRLVLHYRGSRGEAEALAAELNRARPDSVRLVQADLLDAASLPELAA RAVAAFGRLDGLVNNASSFFPTEVGKIDEAAWHDLMGSNLKAPLFLSQALAPELTRRGGAIVGIADIHVDRPMARHVVYN LAKAGHAQLIRSLAIELAPAVRVNGVAPGVNLWPESEASFDAAGRAAIEATIPLRRTGVPEDIARAVAFLLLDASYVTGQ ILAVDGGRSIVL >Mature_252_residues MLTEQQQDERAVLITGAARRVGAGIARQLHARGLRLVLHYRGSRGEAEALAAELNRARPDSVRLVQADLLDAASLPELAA RAVAAFGRLDGLVNNASSFFPTEVGKIDEAAWHDLMGSNLKAPLFLSQALAPELTRRGGAIVGIADIHVDRPMARHVVYN LAKAGHAQLIRSLAIELAPAVRVNGVAPGVNLWPESEASFDAAGRAAIEATIPLRRTGVPEDIARAVAFLLLDASYVTGQ ILAVDGGRSIVL
Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI19923817, Length=248, Percent_Identity=31.0483870967742, Blast_Score=112, Evalue=3e-25, Organism=Homo sapiens, GI32483357, Length=251, Percent_Identity=32.6693227091633, Blast_Score=106, Evalue=2e-23, Organism=Homo sapiens, GI7705925, Length=243, Percent_Identity=32.9218106995885, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=30.8943089430894, Blast_Score=88, Evalue=8e-18, Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=28.8537549407115, Blast_Score=86, Evalue=2e-17, Organism=Homo sapiens, GI33667109, Length=185, Percent_Identity=30.2702702702703, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI59889578, Length=229, Percent_Identity=29.2576419213974, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=27.6, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI10190704, Length=248, Percent_Identity=29.8387096774194, Blast_Score=67, Evalue=1e-11, Organism=Escherichia coli, GI1787335, Length=242, Percent_Identity=34.297520661157, Blast_Score=94, Evalue=9e-21, Organism=Escherichia coli, GI1789208, Length=188, Percent_Identity=35.6382978723404, Blast_Score=87, Evalue=1e-18, Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=32.6848249027237, Blast_Score=82, Evalue=3e-17, Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=28.7449392712551, Blast_Score=80, Evalue=2e-16, Organism=Escherichia coli, GI1787905, Length=243, Percent_Identity=29.6296296296296, Blast_Score=78, Evalue=6e-16, Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=27.9527559055118, Blast_Score=71, Evalue=7e-14, Organism=Escherichia coli, GI1787891, Length=243, Percent_Identity=26.7489711934156, Blast_Score=71, Evalue=8e-14, Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=27.6679841897233, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1790717, Length=245, Percent_Identity=28.1632653061224, Blast_Score=70, Evalue=2e-13, Organism=Escherichia coli, GI1787545, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=2e-13, Organism=Escherichia coli, GI2367365, Length=242, Percent_Identity=29.3388429752066, Blast_Score=62, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=31.5789473684211, Blast_Score=113, Evalue=9e-26, Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=30.5660377358491, Blast_Score=97, Evalue=9e-21, Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=31.0483870967742, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17560676, Length=244, Percent_Identity=30.327868852459, Blast_Score=92, Evalue=3e-19, Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=28.996282527881, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17562906, Length=261, Percent_Identity=29.5019157088123, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=28.1368821292776, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17551412, Length=264, Percent_Identity=28.030303030303, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17563726, Length=257, Percent_Identity=28.4046692607004, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17562990, Length=260, Percent_Identity=28.4615384615385, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=29.8892988929889, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17561402, Length=247, Percent_Identity=28.7449392712551, Blast_Score=84, Evalue=5e-17, Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=30.1204819277108, Blast_Score=84, Evalue=6e-17, Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=28.6290322580645, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17560150, Length=260, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=29.277566539924, Blast_Score=82, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=28.8973384030418, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI17555706, Length=253, Percent_Identity=29.6442687747036, Blast_Score=77, Evalue=8e-15, Organism=Caenorhabditis elegans, GI17531453, Length=265, Percent_Identity=28.6792452830189, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17544670, Length=261, Percent_Identity=27.2030651340996, Blast_Score=75, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17565030, Length=267, Percent_Identity=26.5917602996255, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=27.7566539923954, Blast_Score=70, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17538486, Length=266, Percent_Identity=26.6917293233083, Blast_Score=67, Evalue=7e-12, Organism=Caenorhabditis elegans, GI17560220, Length=266, Percent_Identity=26.6917293233083, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI25147288, Length=246, Percent_Identity=31.3008130081301, Blast_Score=66, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=6e-14, Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=7e-23, Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=30.9236947791165, Blast_Score=96, Evalue=3e-20, Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=32.2834645669291, Blast_Score=92, Evalue=3e-19, Organism=Drosophila melanogaster, GI21357041, Length=252, Percent_Identity=31.3492063492063, Blast_Score=90, Evalue=1e-18, Organism=Drosophila melanogaster, GI24643142, Length=244, Percent_Identity=29.0983606557377, Blast_Score=83, Evalue=1e-16, Organism=Drosophila melanogaster, GI24644337, Length=199, Percent_Identity=34.1708542713568, Blast_Score=80, Evalue=1e-15, Organism=Drosophila melanogaster, GI21355319, Length=245, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=5e-15, Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=27.2357723577236, Blast_Score=64, Evalue=8e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.100 [C]
Molecular weight: Translated: 26703; Mature: 26703
Theoretical pI: Translated: 7.95; Mature: 7.95
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLTEQQQDERAVLITGAARRVGAGIARQLHARGLRLVLHYRGSRGEAEALAAELNRARPD CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCC SVRLVQADLLDAASLPELAARAVAAFGRLDGLVNNASSFFPTEVGKIDEAAWHDLMGSNL HHEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCC KAPLFLSQALAPELTRRGGAIVGIADIHVDRPMARHVVYNLAKAGHAQLIRSLAIELAPA CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC VRVNGVAPGVNLWPESEASFDAAGRAAIEATIPLRRTGVPEDIARAVAFLLLDASYVTGQ EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHCCE ILAVDGGRSIVL EEEECCCCEECC >Mature Secondary Structure MLTEQQQDERAVLITGAARRVGAGIARQLHARGLRLVLHYRGSRGEAEALAAELNRARPD CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCC SVRLVQADLLDAASLPELAARAVAAFGRLDGLVNNASSFFPTEVGKIDEAAWHDLMGSNL HHEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCC KAPLFLSQALAPELTRRGGAIVGIADIHVDRPMARHVVYNLAKAGHAQLIRSLAIELAPA CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC VRVNGVAPGVNLWPESEASFDAAGRAAIEATIPLRRTGVPEDIARAVAFLLLDASYVTGQ EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHCCE ILAVDGGRSIVL EEEECCCCEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NADPH [C]
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]
Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]
General reaction: Redox reaction [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 2583128; 9831526 [H]