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The map label for this gene is ipk

Identifier: 34499514

GI number: 34499514

Start: 4389349

End: 4390203

Strand: Reverse

Name: ipk

Synonym: CV_4059

Alternate gene names: 34499514

Gene position: 4390203-4389349 (Counterclockwise)

Preceding gene: 34499515

Following gene: 34499513

Centisome position: 92.4

GC content: 60.58

Gene sequence:

>855_bases
ATGCAGCATCCGTTTCATTCCTACCCGGCTCCGGCCAAGCTCAACCTTCTGCTGCACGTGGTCGGCAAGAGGCCCGACGG
CTACCACCTGCTGGAAACGGTGTTCCGTTTCATCGATTTCGGCGATACGCTGGAGTTGGCCGTCCGCGACGACGGCCAGA
TCGTTCTGCTGACGCCGACCGACGGCGTGCCGCCGGAGCAGGACCTGACGGTCCGCGCCGCGCGCCTGCTGCAGCGGGAA
AGCGGCTGCCGTTTGGGCGCCAGCATCAAATTGGAGAAGCGCACGCCGATGGGCGGCGGCCTGGGCGGCGGCAGTTCCGA
CGCCGCCACCGCGCTGATCGCGCTGAACCGGCTGTGGGGGCTGGCCTGGCCGCGCGAGCGCTTGCAGGCGCTGGGCCTGC
AGCTGGGCGCCGACGTGCCGGTTTTCATCTTCGGCCGCAACGCGTTGGCCACCGGCGTCGGCGAGGTGTTGGAGCCGATC
CCGCTGAAGCCCGCCTGGTATTTGGTAATTCACCCACAGGTCCATGTGTCGACCATTGAGGTATTCCGAAATTTTTCGCA
AACCGTGTTGACAGAGATAGGACGCGTCGGCATAATGCGAATCCTCGAAACAACGCAGCAGCGCAGAAATGACCTGCAAA
GCGTTGTAGAGAAAAGATTTCCAGCGGTGAATGAAGTACTGAGCGAGTTGAGAAAATATGGTTCGCCGCTGATGACTGGT
TCCGGATCCTGCGTGTTCCTGGAATTCGAGTCGAAAGACGAAGCCGATAAAGTTTACCGGGTGTTGTCGCAAAAATATCA
GGGATTTGTAGCGGAAGGACTGGATGTCCACCCCCTGTTCGACAGCGCTGAATAA

Upstream 100 bases:

>100_bases
GATGCAGCGCGAAGGCCTGACCGTCAAGGTGGTGGTGCAGAGCTGGCAGTGAGCCGCGCGCGCCGCGTCCCGATACCATT
CAATACAGATCAAGATCGAC

Downstream 100 bases:

>100_bases
GGTTACTGGGGAGTCGCCAAGTGGTAAGGCACCGGATTTTGATTCCGGCATTCGTAGGTTCGATCCCTACCTCCCCAGCC
AGGACTTTCTCCGAATAAGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQHPFHSYPAPAKLNLLLHVVGKRPDGYHLLETVFRFIDFGDTLELAVRDDGQIVLLTPTDGVPPEQDLTVRAARLLQRE
SGCRLGASIKLEKRTPMGGGLGGGSSDAATALIALNRLWGLAWPRERLQALGLQLGADVPVFIFGRNALATGVGEVLEPI
PLKPAWYLVIHPQVHVSTIEVFRNFSQTVLTEIGRVGIMRILETTQQRRNDLQSVVEKRFPAVNEVLSELRKYGSPLMTG
SGSCVFLEFESKDEADKVYRVLSQKYQGFVAEGLDVHPLFDSAE

Sequences:

>Translated_284_residues
MQHPFHSYPAPAKLNLLLHVVGKRPDGYHLLETVFRFIDFGDTLELAVRDDGQIVLLTPTDGVPPEQDLTVRAARLLQRE
SGCRLGASIKLEKRTPMGGGLGGGSSDAATALIALNRLWGLAWPRERLQALGLQLGADVPVFIFGRNALATGVGEVLEPI
PLKPAWYLVIHPQVHVSTIEVFRNFSQTVLTEIGRVGIMRILETTQQRRNDLQSVVEKRFPAVNEVLSELRKYGSPLMTG
SGSCVFLEFESKDEADKVYRVLSQKYQGFVAEGLDVHPLFDSAE
>Mature_284_residues
MQHPFHSYPAPAKLNLLLHVVGKRPDGYHLLETVFRFIDFGDTLELAVRDDGQIVLLTPTDGVPPEQDLTVRAARLLQRE
SGCRLGASIKLEKRTPMGGGLGGGSSDAATALIALNRLWGLAWPRERLQALGLQLGADVPVFIFGRNALATGVGEVLEPI
PLKPAWYLVIHPQVHVSTIEVFRNFSQTVLTEIGRVGIMRILETTQQRRNDLQSVVEKRFPAVNEVLSELRKYGSPLMTG
SGSCVFLEFESKDEADKVYRVLSQKYQGFVAEGLDVHPLFDSAE

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=47.6868327402135, Blast_Score=228, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CHRVO (Q7NQS8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903729.1
- ProteinModelPortal:   Q7NQS8
- SMR:   Q7NQS8
- GeneID:   2550097
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4059
- NMPDR:   fig|243365.1.peg.4059
- HOGENOM:   HBG734593
- OMA:   WIEEAPC
- PhylomeDB:   Q7NQS8
- ProtClustDB:   PRK00343
- BioCyc:   CVIO243365:CV_4059-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31381; Mature: 31381

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHPFHSYPAPAKLNLLLHVVGKRPDGYHLLETVFRFIDFGDTLELAVRDDGQIVLLTPT
CCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECC
DGVPPEQDLTVRAARLLQRESGCRLGASIKLEKRTPMGGGLGGGSSDAATALIALNRLWG
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHC
LAWPRERLQALGLQLGADVPVFIFGRNALATGVGEVLEPIPLKPAWYLVIHPQVHVSTIE
CCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHH
VFRNFSQTVLTEIGRVGIMRILETTQQRRNDLQSVVEKRFPAVNEVLSELRKYGSPLMTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEC
SGSCVFLEFESKDEADKVYRVLSQKYQGFVAEGLDVHPLFDSAE
CCCEEEEEECCCCCHHHHHHHHHHHHCCHHHCCCEECCCCCCCC
>Mature Secondary Structure
MQHPFHSYPAPAKLNLLLHVVGKRPDGYHLLETVFRFIDFGDTLELAVRDDGQIVLLTPT
CCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECC
DGVPPEQDLTVRAARLLQRESGCRLGASIKLEKRTPMGGGLGGGSSDAATALIALNRLWG
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHC
LAWPRERLQALGLQLGADVPVFIFGRNALATGVGEVLEPIPLKPAWYLVIHPQVHVSTIE
CCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHH
VFRNFSQTVLTEIGRVGIMRILETTQQRRNDLQSVVEKRFPAVNEVLSELRKYGSPLMTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEC
SGSCVFLEFESKDEADKVYRVLSQKYQGFVAEGLDVHPLFDSAE
CCCEEEEEECCCCCHHHHHHHHHHHHCCHHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782