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The map label for this gene is lolB

Identifier: 34499515

GI number: 34499515

Start: 4390252

End: 4390794

Strand: Reverse

Name: lolB

Synonym: CV_4060

Alternate gene names: 34499515

Gene position: 4390794-4390252 (Counterclockwise)

Preceding gene: 34499516

Following gene: 34499514

Centisome position: 92.42

GC content: 69.24

Gene sequence:

>543_bases
TTGATCCGGCGCGTCCTGCTGCTGTCGCTGGCGTTGCTGTTGGCCGGCTGCGCCAACGAGGCGTTGTTCCGGCCCAAGCC
GGCGCTGGAGTCGGCCGCAGACGCGCCCTTCAGCGTCAGCGGCCGGCTGTCGGCCAATCTGGACGGCAAGGGCCACGTCG
CCAATTTCGACTGGCGGCACCTGCCGCCGCGGGACGAAGTGGCGATCAATTCGCCGCTGGGCAACACCGTGGCCAAGGTG
CTGCGCGATCCCGGCGGCGTCACGCTGTTGGCCGACGGCAAGCGCTGGCAGGCGGACGACGTGGAAAGCCTGACCCGGCA
GGTGATGGGCTGGCCGCTGCCGCTGTCCAACCTGGTATGGTGGATACGCGGCCTGCCGGCGCCCGGCGTGGAGAGCCGGG
TCGACGCCGACGGCAATCTGGAGCAGCAGGGCTGGCACATCCGTTTCATCCGCGACGCCGACGTAGACAGCGACCATCCC
AAGCGGGTGGAGATGCAGCGCGAAGGCCTGACCGTCAAGGTGGTGGTGCAGAGCTGGCAGTGA

Upstream 100 bases:

>100_bases
GCCGCCGCGACGAGGCGCTGGCGGTGTGGCGCAAGGAGCTGGCGCAGCACCCCGGGCATGAGGTGATCGCCGAGACGATG
CGGCGCCTGGGAGCCAAGCC

Downstream 100 bases:

>100_bases
GCCGCGCGCGCCGCGTCCCGATACCATTCAATACAGATCAAGATCGACATGCAGCATCCGTTTCATTCCTACCCGGCTCC
GGCCAAGCTCAACCTTCTGC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MIRRVLLLSLALLLAGCANEALFRPKPALESAADAPFSVSGRLSANLDGKGHVANFDWRHLPPRDEVAINSPLGNTVAKV
LRDPGGVTLLADGKRWQADDVESLTRQVMGWPLPLSNLVWWIRGLPAPGVESRVDADGNLEQQGWHIRFIRDADVDSDHP
KRVEMQREGLTVKVVVQSWQ

Sequences:

>Translated_180_residues
MIRRVLLLSLALLLAGCANEALFRPKPALESAADAPFSVSGRLSANLDGKGHVANFDWRHLPPRDEVAINSPLGNTVAKV
LRDPGGVTLLADGKRWQADDVESLTRQVMGWPLPLSNLVWWIRGLPAPGVESRVDADGNLEQQGWHIRFIRDADVDSDHP
KRVEMQREGLTVKVVVQSWQ
>Mature_180_residues
MIRRVLLLSLALLLAGCANEALFRPKPALESAADAPFSVSGRLSANLDGKGHVANFDWRHLPPRDEVAINSPLGNTVAKV
LRDPGGVTLLADGKRWQADDVESLTRQVMGWPLPLSNLVWWIRGLPAPGVESRVDADGNLEQQGWHIRFIRDADVDSDHP
KRVEMQREGLTVKVVVQSWQ

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_CHRVO (Q7NQS7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903730.1
- ProteinModelPortal:   Q7NQS7
- SMR:   Q7NQS7
- GeneID:   2551229
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4060
- NMPDR:   fig|243365.1.peg.4060
- HOGENOM:   HBG451855
- OMA:   PPVETIN
- PhylomeDB:   Q7NQS7
- ProtClustDB:   PRK00022
- BioCyc:   CVIO243365:CV_4060-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 19895; Mature: 19895

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRVLLLSLALLLAGCANEALFRPKPALESAADAPFSVSGRLSANLDGKGHVANFDWRH
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEECCEEEECCCCCCCCCCCCCCC
LPPRDEVAINSPLGNTVAKVLRDPGGVTLLADGKRWQADDVESLTRQVMGWPLPLSNLVW
CCCCCCEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
WIRGLPAPGVESRVDADGNLEQQGWHIRFIRDADVDSDHPKRVEMQREGLTVKVVVQSWQ
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MIRRVLLLSLALLLAGCANEALFRPKPALESAADAPFSVSGRLSANLDGKGHVANFDWRH
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEECCEEEECCCCCCCCCCCCCCC
LPPRDEVAINSPLGNTVAKVLRDPGGVTLLADGKRWQADDVESLTRQVMGWPLPLSNLVW
CCCCCCEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
WIRGLPAPGVESRVDADGNLEQQGWHIRFIRDADVDSDHPKRVEMQREGLTVKVVVQSWQ
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782