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The map label for this gene is prsA

Identifier: 34499513

GI number: 34499513

Start: 4388195

End: 4389178

Strand: Reverse

Name: prsA

Synonym: CV_4058

Alternate gene names: 34499513

Gene position: 4389178-4388195 (Counterclockwise)

Preceding gene: 34499514

Following gene: 34499512

Centisome position: 92.38

GC content: 62.91

Gene sequence:

>984_bases
ATGGCGGCATACGACAGTTTGATGGTATTTACCGGTACGGCTAATCCGGAACTTGCTCAGAACGTAGTCAAACATTTGGA
TATTTCGTTGGGACGCGCCGATGTCGGCAAGTTCAGCGACGGCGAAGTGGCGGTGGAACTGCTGGAAAACGTTCGCGGCC
GCGACGTTTTCATTCTGCAATCCACCTGTGCGCCGACCAACGACAACCTGATGGAAATCCTGACCATGGCCGACGCGCTC
AAGCGCGCTTCCGCCGGTCGGATCACCGCGGCGATTCCGTATTTCGGCTACGCCCGCCAGGATCGCCGTCCGCGTTCCGC
CCGCGTGCCGATCTCGGCCAAGCTGGTGGCCAATATGCTGACCAGCGCCGGCATCGACCGCGTGCTCACCGTCGACCTGC
ATGCCGACCAGATCCAGGGCTTCTTCGACATCCCGGTGGACAACGTGTATGCGACCCCGGTGTTGCTGAAAGACATCCGC
GCCCAGCGCTTCGACGATCTGATCGTGGTCAGCCCGGACGTGGGCGGCGTGGTCCGCGCCCGCGCGGTGGCCAAGGCGCT
GAACACCGACCTGGCGATCATCGACAAGCGCCGTCCGAAAGCCAATGTGGCCGAAGTGATGAACATCATCGGCGACGTGT
CGGGCCGTACCTGCCTGATCGTCGACGACATGATCGACACCGCCAACACGCTGTGCAAGGCGGCCAGCGCTCTGAAGGAG
CGCGGCGCCGAGCGCGTGCTGGCCTATGCGACCCACGCCATCTTCTCCGGACAGGCGGTGGATCGCATCAAGAATTCCGA
CATCGACATGGTGGTGGTGACCGACACCATCCCGTTGACCGCCGCGGCCAAGGCCTGCCCGAACATCCGGGTCGCCTCGA
TCGCCGGCCTGCTGGCGGAGACGCTGCGCAGGATCAACAACGAAGAATCGGTGTCCTACCTCTTCAATGAGGAACTGGTT
GCGACGGGCGCTTGCCTGCCGTGA

Upstream 100 bases:

>100_bases
CTCCCCAGCCAGGACTTTCTCCGAATAAGAGAGAGCATGAAAAAAGCGTGTAAGAGAGCCTTACACGCTTTTTCTTTATC
CAGCTAAGGCAAAGCGAATC

Downstream 100 bases:

>100_bases
ACATCTGGCTCGCTGGTCGCGGCGGGCCGGGTGCTTTTAATCTGAAACTGGAGTTTTGACATGTCTTACGAACTGATCGC
CGCCAAGCGCGTTGACCTGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAAYDSLMVFTGTANPELAQNVVKHLDISLGRADVGKFSDGEVAVELLENVRGRDVFILQSTCAPTNDNLMEILTMADAL
KRASAGRITAAIPYFGYARQDRRPRSARVPISAKLVANMLTSAGIDRVLTVDLHADQIQGFFDIPVDNVYATPVLLKDIR
AQRFDDLIVVSPDVGGVVRARAVAKALNTDLAIIDKRRPKANVAEVMNIIGDVSGRTCLIVDDMIDTANTLCKAASALKE
RGAERVLAYATHAIFSGQAVDRIKNSDIDMVVVTDTIPLTAAAKACPNIRVASIAGLLAETLRRINNEESVSYLFNEELV
ATGACLP

Sequences:

>Translated_327_residues
MAAYDSLMVFTGTANPELAQNVVKHLDISLGRADVGKFSDGEVAVELLENVRGRDVFILQSTCAPTNDNLMEILTMADAL
KRASAGRITAAIPYFGYARQDRRPRSARVPISAKLVANMLTSAGIDRVLTVDLHADQIQGFFDIPVDNVYATPVLLKDIR
AQRFDDLIVVSPDVGGVVRARAVAKALNTDLAIIDKRRPKANVAEVMNIIGDVSGRTCLIVDDMIDTANTLCKAASALKE
RGAERVLAYATHAIFSGQAVDRIKNSDIDMVVVTDTIPLTAAAKACPNIRVASIAGLLAETLRRINNEESVSYLFNEELV
ATGACLP
>Mature_326_residues
AAYDSLMVFTGTANPELAQNVVKHLDISLGRADVGKFSDGEVAVELLENVRGRDVFILQSTCAPTNDNLMEILTMADALK
RASAGRITAAIPYFGYARQDRRPRSARVPISAKLVANMLTSAGIDRVLTVDLHADQIQGFFDIPVDNVYATPVLLKDIRA
QRFDDLIVVSPDVGGVVRARAVAKALNTDLAIIDKRRPKANVAEVMNIIGDVSGRTCLIVDDMIDTANTLCKAASALKER
GAERVLAYATHAIFSGQAVDRIKNSDIDMVVVTDTIPLTAAAKACPNIRVASIAGLLAETLRRINNEESVSYLFNEELVA
TGACLP

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=51.2738853503185, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=50.6369426751592, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=50, Blast_Score=314, Evalue=6e-86,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=50.9554140127389, Blast_Score=314, Evalue=8e-86,
Organism=Homo sapiens, GI194018537, Length=331, Percent_Identity=38.0664652567976, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI4506133, Length=345, Percent_Identity=35.9420289855072, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=67.6282051282051, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=48.4076433121019, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17554704, Length=314, Percent_Identity=48.7261146496815, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=48.4076433121019, Blast_Score=307, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=48.4076433121019, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17570245, Length=344, Percent_Identity=33.4302325581395, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319403, Length=319, Percent_Identity=46.0815047021944, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321776, Length=300, Percent_Identity=48, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320946, Length=316, Percent_Identity=43.9873417721519, Blast_Score=265, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=41.3265306122449, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=114, Percent_Identity=42.1052631578947, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=51.1182108626198, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=47.7611940298507, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=34.180790960452, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=34.180790960452, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=34.3661971830986, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=34.3661971830986, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24651462, Length=202, Percent_Identity=35.6435643564356, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24651464, Length=202, Percent_Identity=35.6435643564356, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI45552010, Length=203, Percent_Identity=35.9605911330049, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_CHRVO (Q7NQS9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903728.1
- ProteinModelPortal:   Q7NQS9
- SMR:   Q7NQS9
- GeneID:   2550095
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4058
- NMPDR:   fig|243365.1.peg.4058
- HOGENOM:   HBG519284
- OMA:   YKTAGAD
- PhylomeDB:   Q7NQS9
- ProtClustDB:   PRK01259
- BioCyc:   CVIO243365:CV_4058-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35195; Mature: 35064

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYDSLMVFTGTANPELAQNVVKHLDISLGRADVGKFSDGEVAVELLENVRGRDVFILQ
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEE
STCAPTNDNLMEILTMADALKRASAGRITAAIPYFGYARQDRRPRSARVPISAKLVANML
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHH
TSAGIDRVLTVDLHADQIQGFFDIPVDNVYATPVLLKDIRAQRFDDLIVVSPDVGGVVRA
HHCCCCEEEEEEEEHHHCCCEEECCCCCEEECHHHHHHHHHHHCCCEEEECCCCCHHHHH
RAVAKALNTDLAIIDKRRPKANVAEVMNIIGDVSGRTCLIVDDMIDTANTLCKAASALKE
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RGAERVLAYATHAIFSGQAVDRIKNSDIDMVVVTDTIPLTAAAKACPNIRVASIAGLLAE
CCHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHH
TLRRINNEESVSYLFNEELVATGACLP
HHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AAYDSLMVFTGTANPELAQNVVKHLDISLGRADVGKFSDGEVAVELLENVRGRDVFILQ
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEE
STCAPTNDNLMEILTMADALKRASAGRITAAIPYFGYARQDRRPRSARVPISAKLVANML
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHH
TSAGIDRVLTVDLHADQIQGFFDIPVDNVYATPVLLKDIRAQRFDDLIVVSPDVGGVVRA
HHCCCCEEEEEEEEHHHCCCEEECCCCCEEECHHHHHHHHHHHCCCEEEECCCCCHHHHH
RAVAKALNTDLAIIDKRRPKANVAEVMNIIGDVSGRTCLIVDDMIDTANTLCKAASALKE
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RGAERVLAYATHAIFSGQAVDRIKNSDIDMVVVTDTIPLTAAAKACPNIRVASIAGLLAE
CCHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHH
TLRRINNEESVSYLFNEELVATGACLP
HHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782