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The map label for this gene is alaXM [H]

Identifier: 34499208

GI number: 34499208

Start: 4050960

End: 4051667

Strand: Direct

Name: alaXM [H]

Synonym: CV_3753

Alternate gene names: 34499208

Gene position: 4050960-4051667 (Clockwise)

Preceding gene: 34499205

Following gene: 34499209

Centisome position: 85.26

GC content: 67.09

Gene sequence:

>708_bases
ATGAGCGAACAGTTCTACGCCAATGCCTATCAAACCCGCCTCGCCACCCGCGTGCTGCGTCACGACGACGCCGGCCTGAT
CCTGGAAGACACGCTGTGCTACCCGCTCGGCGGCGGCCAGCCCGGCGACAGCGCCGCGTTGACGCTGGCGGACCGCTCCG
CGCTGCGCATCGCAGACACCCGCCGCGACCGCGAAACCCGGGCCATCCTGCACCAGACAGCAGGCGACGCCCGTCTCGCG
CCGGGAACGGAAGTGACGCTGGAGCTGGACTGGGACCGCCGCTACCGCCACATGCGCATCCACACCTGTCTGCATTTGCT
GGGCGTGGTGGTCAAGGCCGGCGTCACCGGCGGCAACATGACCGCCGAATCGGGCCGGCTGGACTTCGCGCTGCCCGAGG
GCATGGAGCTGGACAAGGAAAACATCGAGGCCGAATTGAACCGCCTGGTGGACGCCGACCTGCCCCTCGCGGTGAGGATA
ACCAGCGGCGAAGCGTTGAAGGCGCAGCCTGAGCTGATCCGCACCATGTCGGTGACGCCGCCGCTGCATCTGCCGGAAAT
CCGTCTGATCGAAATCGAAGGCGTGGATCTGCAGCCCTGCGGCGGCACCCATGTCGCGCGCACCGGCGAAGTGGGCCGGG
TCAGGGTGAAGAAGATAGAAAGCAAGGGCGCGCGCAACAAGCGCGTGGTGGTGGAATTGGTCGACTGA

Upstream 100 bases:

>100_bases
CGCATCCAGGGTCCAAGAATGACGGCCATGCCCCGACCAGACCAATAATTTAGTTTTAGTTACTATAGTTCATGAAAATA
GTTAAAAACAAGGAAATCAC

Downstream 100 bases:

>100_bases
CGCCGGGAGAAACGCCATGGACGAAACCGTGATCGGCAGCGTGCTCGCCGTTCTGGCAGGCAAAGCCGTGCCCTATGCCC
GCCCCGGCGCGCTCAGCGCC

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSEQFYANAYQTRLATRVLRHDDAGLILEDTLCYPLGGGQPGDSAALTLADRSALRIADTRRDRETRAILHQTAGDARLA
PGTEVTLELDWDRRYRHMRIHTCLHLLGVVVKAGVTGGNMTAESGRLDFALPEGMELDKENIEAELNRLVDADLPLAVRI
TSGEALKAQPELIRTMSVTPPLHLPEIRLIEIEGVDLQPCGGTHVARTGEVGRVRVKKIESKGARNKRVVVELVD

Sequences:

>Translated_235_residues
MSEQFYANAYQTRLATRVLRHDDAGLILEDTLCYPLGGGQPGDSAALTLADRSALRIADTRRDRETRAILHQTAGDARLA
PGTEVTLELDWDRRYRHMRIHTCLHLLGVVVKAGVTGGNMTAESGRLDFALPEGMELDKENIEAELNRLVDADLPLAVRI
TSGEALKAQPELIRTMSVTPPLHLPEIRLIEIEGVDLQPCGGTHVARTGEVGRVRVKKIESKGARNKRVVVELVD
>Mature_234_residues
SEQFYANAYQTRLATRVLRHDDAGLILEDTLCYPLGGGQPGDSAALTLADRSALRIADTRRDRETRAILHQTAGDARLAP
GTEVTLELDWDRRYRHMRIHTCLHLLGVVVKAGVTGGNMTAESGRLDFALPEGMELDKENIEAELNRLVDADLPLAVRIT
SGEALKAQPELIRTMSVTPPLHLPEIRLIEIEGVDLQPCGGTHVARTGEVGRVRVKKIESKGARNKRVVVELVD

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) or Gly-tRNA(Ala). Has no activity on incorrectly charged Ser-tRNA(Thr), nor on correctly charged Ala-tRNA(Ala) or Ser-tRNA(Ser) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=216, Percent_Identity=32.4074074074074, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI13376886, Length=216, Percent_Identity=32.4074074074074, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1789048, Length=202, Percent_Identity=26.2376237623762, Blast_Score=60, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324288, Length=210, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 25856; Mature: 25725

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFYANAYQTRLATRVLRHDDAGLILEDTLCYPLGGGQPGDSAALTLADRSALRIADT
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCCCCCCEEEEEECCCEEEEHHC
RRDRETRAILHQTAGDARLAPGTEVTLELDWDRRYRHMRIHTCLHLLGVVVKAGVTGGNM
CCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
TAESGRLDFALPEGMELDKENIEAELNRLVDADLPLAVRITSGEALKAQPELIRTMSVTP
ECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHEEECCC
PLHLPEIRLIEIEGVDLQPCGGTHVARTGEVGRVRVKKIESKGARNKRVVVELVD
CCCCCCEEEEEEECCEECCCCCCCEECCCCCCEEEEEEECCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SEQFYANAYQTRLATRVLRHDDAGLILEDTLCYPLGGGQPGDSAALTLADRSALRIADT
CCCHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCCCCCCEEEEEECCCEEEEHHC
RRDRETRAILHQTAGDARLAPGTEVTLELDWDRRYRHMRIHTCLHLLGVVVKAGVTGGNM
CCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
TAESGRLDFALPEGMELDKENIEAELNRLVDADLPLAVRITSGEALKAQPELIRTMSVTP
ECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHEEECCC
PLHLPEIRLIEIEGVDLQPCGGTHVARTGEVGRVRVKKIESKGARNKRVVVELVD
CCCCCCEEEEEEECCEECCCCCCCEECCCCCCEEEEEEECCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA