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The map label for this gene is ppsA [H]

Identifier: 34499164

GI number: 34499164

Start: 4010431

End: 4012818

Strand: Reverse

Name: ppsA [H]

Synonym: CV_3709

Alternate gene names: 34499164

Gene position: 4012818-4010431 (Counterclockwise)

Preceding gene: 34499166

Following gene: 34499159

Centisome position: 84.46

GC content: 64.28

Gene sequence:

>2388_bases
ATGGCAGACAACTACGTCATCTGGTTCGAGAAGCTGCGCATGACCGACGTGGAAGAGGTAGGCGGCAAGAACGCCTCCCT
CGGCGAAATGATCAGTCAGCTGGCGGGCTCCGGCGTTCGTGTTCCCGGCGGCTTTGCCACCACTGCTCAGGCATACCGGG
ATTTCCTGCAGCACAATGGCCTGGCCGACAAGATCAACGCCGCGCTGGCCAAACTCGATATCGACGACGTCAGCGAACTG
GCCCGGGTGGGCAAGGAAATCCGCCAGTGGATCATCGACACCCCGTTCCCGGCCAAGCTGGATGCCGACATCAAGGAAGC
TTGGGACAAGATGGTGGCCGACGCCGGCGGCGCCGACATCTCCGTGGCGGTCCGCTCCTCCGCCACCGCTGAAGACCTCC
CCGACGCCTCGTTCGCAGGCCAGCAAGAAACCTTCCTGAACATCCGCGGCCTCGACAACGTCAAGGACGCGATGAAGCAC
GTGTTCGCCTCCCTGTACAACGACCGCGCCATCTCCTACCGCGTGCACAAGGGCTTTGCCCACGACGTGGTGGCCCTGTC
CGCAGGCGTGCAGCGCATGGTGCGTTCCGACATGGGCGCGGCCGGCGTGATGTTCACCATCGACACCGAATCCGGCTTCG
AGGACGTGGTGTTCATCACCGCTTCCTACGGCCTGGGCGAGACCGTGGTGCAGGGCGCCGTGAACCCGGACGAGTTCTAC
GTGCACAAGCCGACGCTGAAGGCCGGCCGCCCGGCGGTGCTGCGCAAGACCATGGGCTCCAAGCTGATCAAGATGGAGTT
CACCGACGCGTCGCAAGCCGGCCGCTCGGTCAAGACCGTGGATGTGGAGCCGGCGCTGCGCAAAGCGTTCTCGATCAGCG
ACGCCGAAGTGGCTGAACTGGCCCAGTACGCGCTGATCATCGAAAAACACTACGGCCGCCCGATGGACATCGAGTGGGGC
CGCGACGGCGTGGACGGCAAGCTGTACATTCTGCAGGCGCGTCCGGAAACCGTTAAGTCGCAGGAAGACCGCAAGGACAC
CCTGCGCCGCTACCGCCTGAACAGCAAGTCCGAGATCCTGTGCGAAGGCCGCGCCATCGGCCAGAAGATCGGCCAGGGCG
TGGTGCGCACCATCAAGGACGCGTCCGAGATGGACCGCGTCAAGCCGGGCGATGTGCTGGTCACCGACATGACCGACCCG
GATTGGGAACCGGTGATGAAGCGCGCCGCCGCCATCGTGACCAACCGCGGCGGCCGCACCTGCCACGCGGCGATCATCGC
GCGCGAGCTGGGCATCCCGGCCGTGGTCGGCTGCGGCGACGCCACCGACGTGCTGAAGGAAGGCATGCAGGTCACCGTGT
CCTGCGCCGAAGGCGACACCGGCAACATCTACGACGGCCTGCTGGACGTGGAAGTGATCGACCTGGAACTGGACAAGATG
CCCAAGGCCCCGGTCAAGATCATGATGAACGTCGGCAATCCGGAACTGGCTTTCGATTTCGCCCAACTGCCTAACGAAGG
CGTGGGCCTGGCGCGGATGGAATTCGTCATCAACCGCCAGATCGGCATCCACCCGAAAGCGCTGCTGGAGTTCGACAAGC
TGAGCCCGGAGCTGAAGGAAACCATTTCCGACCGCATCGCCGGCTACGCCAGCCCGGTGGACTTCTACGTCGACAAGATC
GCCGAGGGCGTGTCCACGCTGGCCGCGGCCTTCTACCCGAAGAAGGTCATCGTGCGCATGTCGGACTTCAAGTCCAACGA
GTACGCCAACCTGATCGGCGGCCAGCTGTACGAGCCGCACGAAGAGAACCCGATGATCGGCTTCCGCGGCGCCGCCCGCT
ACGTGGCCGAGTCGTTCCGCGACTGTTTCGAGCTGGAATGCCGCGCGATCAAGAAGGTGCGCGACGTGATGGGCCTGACC
AACGTGGAAGTGATGATCCCGTTCGTCCGCACCCTGGCCGAAGCCGAGAAGGTGGTCGAGATCCTGGCTGAAAACGGCCT
GAAGCGCGGCGAAAACGGCTTGCGCCTGATCATGATGTGCGAGCTGCCGACCAACGCGGTGCTGGCTGAGAAGTTCCTGC
AGCACTTCGACGGCTTCTCCATCGGCTCCAACGACATGACCCAGCTGACGCTGGGCGTGGACCGCGATTCCGGCGGCCCG
ATCGCCTCCACCTTCGACGAGCGCAACGAGGCGGTCAAGGCCATGCTGCACATGTCCATCCAAGCTTGCCGCAAGCTGGG
CAAGTACGTGGGCATCTGCGGCCAGGGCCCGTCCGACCACCCGGACTTCGCCAAGTGGCTGGTGGAGGAGGGCATCGAGA
CCGTGTCGCTGAACCCGGACACCGTGGTGGAAACCTGGCTTTACCTGGCCAAGGAGCTGAACAGCTGA

Upstream 100 bases:

>100_bases
GCAAAAAGTCTTGTGCAGCGCAAAAGATGCTACGCAATTCGCACGTAATTCGGCTAGAATGCGGCCCTATCGTCCCAATC
CCAACACAGGAAGTGAAGTG

Downstream 100 bases:

>100_bases
GGCTGACGGCAGCCCATCGGTGAATGAGAAACCGCCCGGAAACGGGCGGTTTTCGCTTGTTTGCGGCAGGCTGATGATCA
GCGGCCGGGGTCGCCGTTCC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MADNYVIWFEKLRMTDVEEVGGKNASLGEMISQLAGSGVRVPGGFATTAQAYRDFLQHNGLADKINAALAKLDIDDVSEL
ARVGKEIRQWIIDTPFPAKLDADIKEAWDKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNIRGLDNVKDAMKH
VFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDMGAAGVMFTIDTESGFEDVVFITASYGLGETVVQGAVNPDEFY
VHKPTLKAGRPAVLRKTMGSKLIKMEFTDASQAGRSVKTVDVEPALRKAFSISDAEVAELAQYALIIEKHYGRPMDIEWG
RDGVDGKLYILQARPETVKSQEDRKDTLRRYRLNSKSEILCEGRAIGQKIGQGVVRTIKDASEMDRVKPGDVLVTDMTDP
DWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATDVLKEGMQVTVSCAEGDTGNIYDGLLDVEVIDLELDKM
PKAPVKIMMNVGNPELAFDFAQLPNEGVGLARMEFVINRQIGIHPKALLEFDKLSPELKETISDRIAGYASPVDFYVDKI
AEGVSTLAAAFYPKKVIVRMSDFKSNEYANLIGGQLYEPHEENPMIGFRGAARYVAESFRDCFELECRAIKKVRDVMGLT
NVEVMIPFVRTLAEAEKVVEILAENGLKRGENGLRLIMMCELPTNAVLAEKFLQHFDGFSIGSNDMTQLTLGVDRDSGGP
IASTFDERNEAVKAMLHMSIQACRKLGKYVGICGQGPSDHPDFAKWLVEEGIETVSLNPDTVVETWLYLAKELNS

Sequences:

>Translated_795_residues
MADNYVIWFEKLRMTDVEEVGGKNASLGEMISQLAGSGVRVPGGFATTAQAYRDFLQHNGLADKINAALAKLDIDDVSEL
ARVGKEIRQWIIDTPFPAKLDADIKEAWDKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNIRGLDNVKDAMKH
VFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDMGAAGVMFTIDTESGFEDVVFITASYGLGETVVQGAVNPDEFY
VHKPTLKAGRPAVLRKTMGSKLIKMEFTDASQAGRSVKTVDVEPALRKAFSISDAEVAELAQYALIIEKHYGRPMDIEWG
RDGVDGKLYILQARPETVKSQEDRKDTLRRYRLNSKSEILCEGRAIGQKIGQGVVRTIKDASEMDRVKPGDVLVTDMTDP
DWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATDVLKEGMQVTVSCAEGDTGNIYDGLLDVEVIDLELDKM
PKAPVKIMMNVGNPELAFDFAQLPNEGVGLARMEFVINRQIGIHPKALLEFDKLSPELKETISDRIAGYASPVDFYVDKI
AEGVSTLAAAFYPKKVIVRMSDFKSNEYANLIGGQLYEPHEENPMIGFRGAARYVAESFRDCFELECRAIKKVRDVMGLT
NVEVMIPFVRTLAEAEKVVEILAENGLKRGENGLRLIMMCELPTNAVLAEKFLQHFDGFSIGSNDMTQLTLGVDRDSGGP
IASTFDERNEAVKAMLHMSIQACRKLGKYVGICGQGPSDHPDFAKWLVEEGIETVSLNPDTVVETWLYLAKELNS
>Mature_794_residues
ADNYVIWFEKLRMTDVEEVGGKNASLGEMISQLAGSGVRVPGGFATTAQAYRDFLQHNGLADKINAALAKLDIDDVSELA
RVGKEIRQWIIDTPFPAKLDADIKEAWDKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNIRGLDNVKDAMKHV
FASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDMGAAGVMFTIDTESGFEDVVFITASYGLGETVVQGAVNPDEFYV
HKPTLKAGRPAVLRKTMGSKLIKMEFTDASQAGRSVKTVDVEPALRKAFSISDAEVAELAQYALIIEKHYGRPMDIEWGR
DGVDGKLYILQARPETVKSQEDRKDTLRRYRLNSKSEILCEGRAIGQKIGQGVVRTIKDASEMDRVKPGDVLVTDMTDPD
WEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATDVLKEGMQVTVSCAEGDTGNIYDGLLDVEVIDLELDKMP
KAPVKIMMNVGNPELAFDFAQLPNEGVGLARMEFVINRQIGIHPKALLEFDKLSPELKETISDRIAGYASPVDFYVDKIA
EGVSTLAAAFYPKKVIVRMSDFKSNEYANLIGGQLYEPHEENPMIGFRGAARYVAESFRDCFELECRAIKKVRDVMGLTN
VEVMIPFVRTLAEAEKVVEILAENGLKRGENGLRLIMMCELPTNAVLAEKFLQHFDGFSIGSNDMTQLTLGVDRDSGGPI
ASTFDERNEAVKAMLHMSIQACRKLGKYVGICGQGPSDHPDFAKWLVEEGIETVSLNPDTVVETWLYLAKELNS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=786, Percent_Identity=66.6666666666667, Blast_Score=1110, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=433, Percent_Identity=26.0969976905312, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=31.7708333333333, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI48994992, Length=373, Percent_Identity=24.9329758713137, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789193, Length=395, Percent_Identity=25.0632911392405, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17564524, Length=316, Percent_Identity=27.2151898734177, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87177; Mature: 87045

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNYVIWFEKLRMTDVEEVGGKNASLGEMISQLAGSGVRVPGGFATTAQAYRDFLQHNG
CCCCEEEEEEHHHHCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
LADKINAALAKLDIDDVSELARVGKEIRQWIIDTPFPAKLDADIKEAWDKMVADAGGADI
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCE
SVAVRSSATAEDLPDASFAGQQETFLNIRGLDNVKDAMKHVFASLYNDRAISYRVHKGFA
EEEEECCCCHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCH
HDVVALSAGVQRMVRSDMGAAGVMFTIDTESGFEDVVFITASYGLGETVVQGAVNPDEFY
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCEE
VHKPTLKAGRPAVLRKTMGSKLIKMEFTDASQAGRSVKTVDVEPALRKAFSISDAEVAEL
EECCCCCCCCHHHHHHHHCCCEEEEEECCHHHCCCCEEEEECCHHHHHHHCCCHHHHHHH
AQYALIIEKHYGRPMDIEWGRDGVDGKLYILQARPETVKSQEDRKDTLRRYRLNSKSEIL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHCCCCCCEE
CEGRAIGQKIGQGVVRTIKDASEMDRVKPGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRT
ECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCH
CHAAIIARELGIPAVVGCGDATDVLKEGMQVTVSCAEGDTGNIYDGLLDVEVIDLELDKM
HHHHHHHHHCCCCEEEECCCHHHHHHCCCEEEEEECCCCCCCHHCCEEEEEEEEEECCCC
PKAPVKIMMNVGNPELAFDFAQLPNEGVGLARMEFVINRQIGIHPKALLEFDKLSPELKE
CCCCEEEEEECCCCHHEEEHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHH
TISDRIAGYASPVDFYVDKIAEGVSTLAAAFYPKKVIVRMSDFKSNEYANLIGGQLYEPH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHCCCCCCCC
EENPMIGFRGAARYVAESFRDCFELECRAIKKVRDVMGLTNVEVMIPFVRTLAEAEKVVE
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ILAENGLKRGENGLRLIMMCELPTNAVLAEKFLQHFDGFSIGSNDMTQLTLGVDRDSGGP
HHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCC
IASTFDERNEAVKAMLHMSIQACRKLGKYVGICGQGPSDHPDFAKWLVEEGIETVSLNPD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEECCCH
TVVETWLYLAKELNS
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADNYVIWFEKLRMTDVEEVGGKNASLGEMISQLAGSGVRVPGGFATTAQAYRDFLQHNG
CCCEEEEEEHHHHCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
LADKINAALAKLDIDDVSELARVGKEIRQWIIDTPFPAKLDADIKEAWDKMVADAGGADI
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCE
SVAVRSSATAEDLPDASFAGQQETFLNIRGLDNVKDAMKHVFASLYNDRAISYRVHKGFA
EEEEECCCCHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCH
HDVVALSAGVQRMVRSDMGAAGVMFTIDTESGFEDVVFITASYGLGETVVQGAVNPDEFY
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCEE
VHKPTLKAGRPAVLRKTMGSKLIKMEFTDASQAGRSVKTVDVEPALRKAFSISDAEVAEL
EECCCCCCCCHHHHHHHHCCCEEEEEECCHHHCCCCEEEEECCHHHHHHHCCCHHHHHHH
AQYALIIEKHYGRPMDIEWGRDGVDGKLYILQARPETVKSQEDRKDTLRRYRLNSKSEIL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHCCCCCCEE
CEGRAIGQKIGQGVVRTIKDASEMDRVKPGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRT
ECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCH
CHAAIIARELGIPAVVGCGDATDVLKEGMQVTVSCAEGDTGNIYDGLLDVEVIDLELDKM
HHHHHHHHHCCCCEEEECCCHHHHHHCCCEEEEEECCCCCCCHHCCEEEEEEEEEECCCC
PKAPVKIMMNVGNPELAFDFAQLPNEGVGLARMEFVINRQIGIHPKALLEFDKLSPELKE
CCCCEEEEEECCCCHHEEEHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHH
TISDRIAGYASPVDFYVDKIAEGVSTLAAAFYPKKVIVRMSDFKSNEYANLIGGQLYEPH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHCCCCCCCC
EENPMIGFRGAARYVAESFRDCFELECRAIKKVRDVMGLTNVEVMIPFVRTLAEAEKVVE
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ILAENGLKRGENGLRLIMMCELPTNAVLAEKFLQHFDGFSIGSNDMTQLTLGVDRDSGGP
HHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCC
IASTFDERNEAVKAMLHMSIQACRKLGKYVGICGQGPSDHPDFAKWLVEEGIETVSLNPD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEECCCH
TVVETWLYLAKELNS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]