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The map label for this gene is mutY [H]

Identifier: 34499158

GI number: 34499158

Start: 4003498

End: 4004541

Strand: Reverse

Name: mutY [H]

Synonym: CV_3703

Alternate gene names: 34499158

Gene position: 4004541-4003498 (Counterclockwise)

Preceding gene: 34499159

Following gene: 34499157

Centisome position: 84.29

GC content: 69.54

Gene sequence:

>1044_bases
TTGCACAGCAGTTTCGCCTCCCGGCTGGTGGCCTGGCAACGGCGGCATGGCCGCCACGACCTGCCCTGGCAGGTGAAGGA
CCCGTATCGGGTGTGGCTGTCAGAGATCATGCTGCAGCAGACCCAGGTGAAGAGCGTGCTCGACTATTACCCGCGTTTCC
TGGCCCGCTTCCCGGACGTGGCCAGCCTGGCCGCCGCGCCGGTGGACGACGTGCTGGCGCAGTGGAGCGGCCTGGGCTAC
TACAGCCGGGCGCGCAATCTGCACAAGGCGGCGAAGATGGTGATGGATGCTTTCGGCGGCGCCTTTCCCCCCGAGCGGGA
GCGGCTGGAGCAGCTGCCCGGCGTCGGGCGCTCCACCGCCGCCGCGATTTCCGCCTTCGCCTTCGGCCGGCGCGAGACCA
TCCTGGACGGCAATGTCAAACGGGTGCTGGCGCGCTGCTTCGGCATAGACGGCTTTCCCGGCGACAAGGCGATAGAGAAG
CGGATGTGGGCGCTGGCCGAGGAGATCCTGCCGGCCGCCGCCGCCGACATCGGCCCCTATGTGCAGGGTCTGATGGACCT
GGGCGCGACGGTGTGCAGCCGCGGCAAGCCGGCCTGCACCGCCTGTCCGATGGTGGACGGCTGCGTGGCGGCGCGGGAGG
GCCGCACCGGCGAACTGCCGACGCCGCGGCCGAAAAAGGCTGTGCCGATCCGGCACACGGCGATGCTGCTGGCGCGGCAT
GAAAACAAGGTATGGCTTGAGCGCCGGCCGCCGACCGGCATCTGGGGCGGGCTGTTGTCGCTGCCGGAGTTCGCCACCAC
CTTGGAAATGGAAGACTGGCTGAGCGGCCGGGGCGATGGCGACATGCTGCCCGCCTGGCCGGAGCTGGAGCATGTGTTTA
CTCATTTCCGCTTGATCATCACGCCGCAGCCGGTCCGGATCGACCGCCTGCATGCGGCGGGAGCCGCCGAGGCCGATGGC
CAGTGGCTGGACATCGATCAGGCTGTGGACGCCGGCGTGCCGGCGCCGGTCCGTCGCCTGCTGCTCAGGCTGGCGTCGGA
TTGA

Upstream 100 bases:

>100_bases
AAGTAATCCTTTAATTTTAACGTATTTGGCCGCCGCCTGCTTGGTGGCGCTGCGGCTTTCCGCTACCATAACCGGTTCGA
TTCGATATTGGATTGCGATG

Downstream 100 bases:

>100_bases
CGAGGGCGGACGAGGGTCCGCCGATTGTGGCAAATGCCGGGCGCGCCCCGGCTTACAATTGAAGACGGCGAGCCCGTCCA
GATAGACAGATATGGTTTCC

Product: A/G-specific DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MHSSFASRLVAWQRRHGRHDLPWQVKDPYRVWLSEIMLQQTQVKSVLDYYPRFLARFPDVASLAAAPVDDVLAQWSGLGY
YSRARNLHKAAKMVMDAFGGAFPPERERLEQLPGVGRSTAAAISAFAFGRRETILDGNVKRVLARCFGIDGFPGDKAIEK
RMWALAEEILPAAAADIGPYVQGLMDLGATVCSRGKPACTACPMVDGCVAAREGRTGELPTPRPKKAVPIRHTAMLLARH
ENKVWLERRPPTGIWGGLLSLPEFATTLEMEDWLSGRGDGDMLPAWPELEHVFTHFRLIITPQPVRIDRLHAAGAAEADG
QWLDIDQAVDAGVPAPVRRLLLRLASD

Sequences:

>Translated_347_residues
MHSSFASRLVAWQRRHGRHDLPWQVKDPYRVWLSEIMLQQTQVKSVLDYYPRFLARFPDVASLAAAPVDDVLAQWSGLGY
YSRARNLHKAAKMVMDAFGGAFPPERERLEQLPGVGRSTAAAISAFAFGRRETILDGNVKRVLARCFGIDGFPGDKAIEK
RMWALAEEILPAAAADIGPYVQGLMDLGATVCSRGKPACTACPMVDGCVAAREGRTGELPTPRPKKAVPIRHTAMLLARH
ENKVWLERRPPTGIWGGLLSLPEFATTLEMEDWLSGRGDGDMLPAWPELEHVFTHFRLIITPQPVRIDRLHAAGAAEADG
QWLDIDQAVDAGVPAPVRRLLLRLASD
>Mature_347_residues
MHSSFASRLVAWQRRHGRHDLPWQVKDPYRVWLSEIMLQQTQVKSVLDYYPRFLARFPDVASLAAAPVDDVLAQWSGLGY
YSRARNLHKAAKMVMDAFGGAFPPERERLEQLPGVGRSTAAAISAFAFGRRETILDGNVKRVLARCFGIDGFPGDKAIEK
RMWALAEEILPAAAADIGPYVQGLMDLGATVCSRGKPACTACPMVDGCVAAREGRTGELPTPRPKKAVPIRHTAMLLARH
ENKVWLERRPPTGIWGGLLSLPEFATTLEMEDWLSGRGDGDMLPAWPELEHVFTHFRLIITPQPVRIDRLHAAGAAEADG
QWLDIDQAVDAGVPAPVRRLLLRLASD

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=218, Percent_Identity=40.3669724770642, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI115298654, Length=218, Percent_Identity=40.3669724770642, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI115298652, Length=218, Percent_Identity=40.3669724770642, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI115298650, Length=218, Percent_Identity=40.3669724770642, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI6912520, Length=219, Percent_Identity=40.6392694063927, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI190358497, Length=219, Percent_Identity=40.6392694063927, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI1789331, Length=344, Percent_Identity=45.9302325581395, Blast_Score=328, Evalue=3e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 38383; Mature: 38383

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSSFASRLVAWQRRHGRHDLPWQVKDPYRVWLSEIMLQQTQVKSVLDYYPRFLARFPDV
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ASLAAAPVDDVLAQWSGLGYYSRARNLHKAAKMVMDAFGGAFPPERERLEQLPGVGRSTA
HHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHH
AAISAFAFGRRETILDGNVKRVLARCFGIDGFPGDKAIEKRMWALAEEILPAAAADIGPY
HHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VQGLMDLGATVCSRGKPACTACPMVDGCVAAREGRTGELPTPRPKKAVPIRHTAMLLARH
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
ENKVWLERRPPTGIWGGLLSLPEFATTLEMEDWLSGRGDGDMLPAWPELEHVFTHFRLII
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEE
TPQPVRIDRLHAAGAAEADGQWLDIDQAVDAGVPAPVRRLLLRLASD
CCCCCCHHHHHCCCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHSSFASRLVAWQRRHGRHDLPWQVKDPYRVWLSEIMLQQTQVKSVLDYYPRFLARFPDV
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ASLAAAPVDDVLAQWSGLGYYSRARNLHKAAKMVMDAFGGAFPPERERLEQLPGVGRSTA
HHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHH
AAISAFAFGRRETILDGNVKRVLARCFGIDGFPGDKAIEKRMWALAEEILPAAAADIGPY
HHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VQGLMDLGATVCSRGKPACTACPMVDGCVAAREGRTGELPTPRPKKAVPIRHTAMLLARH
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
ENKVWLERRPPTGIWGGLLSLPEFATTLEMEDWLSGRGDGDMLPAWPELEHVFTHFRLII
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEE
TPQPVRIDRLHAAGAAEADGQWLDIDQAVDAGVPAPVRRLLLRLASD
CCCCCCHHHHHCCCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]