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The map label for this gene is yaaJ [C]

Identifier: 34499105

GI number: 34499105

Start: 3948733

End: 3950148

Strand: Reverse

Name: yaaJ [C]

Synonym: CV_3650

Alternate gene names: 34499105

Gene position: 3950148-3948733 (Counterclockwise)

Preceding gene: 34499107

Following gene: 34499102

Centisome position: 83.14

GC content: 66.6

Gene sequence:

>1416_bases
ATGGAGTTGCTTCATTCTTTGATCAATGCCGTCAACGACGTGCTGTGGGGCAAGGTGCTGATCGCCCTGCTGTTGGCGGT
GGGTCTGTATTTCTCGCTGCGTTCCGGTTTGTTGCAGCTGAGGCTGTTCTCGCGCGGTTGGCGCGAAATGATGGGCGGCC
GCGGCGGCCATGTCAGCAAGAATGACATCTCGCCGTTCCAGGCCTTCGCCACCGGCCTCGCCAGCCGCGTGGGCACCGGC
AACATCGCCGGCGTCGCCATCGCCATCGCCGCCGGCGGTCCCGGCGCGGTGTTCTGGATGTGGCTGACCGCCTTGCTCGG
CATGTCCAGCGCCTTCGTCGAATCGACGCTGGCCCAGCTGTTCAAGACCAGCCACCACGACGACACTTTCCGCGGCGGCC
CCGCTTACTACATCCAGAAGGGCCTGGGCCTGCGCTGGCTGGGCGTGCTGTTCGCGCTGTGCCTGATCCTGGCTTTCGGC
CTGGTGTTCAACGCGGTGCAGGCCAACTCCATCGTCGCCGCGACCGAAGGCGCCTGGGGCTGGAACCGCTATGCGGTGGG
CGTCGGCCTGGTGCTGTTGACCGCACCGGTGATCTTCGGCGGCGTGCGCTCGGTGGCGCGGGTGGCGGAGTGGCTGGTGC
CGGTGATGGCGCTGATCTACCTGCTGATGGCCTTCTACGTGCTGGCCATCCACGCGGCCGAACTGCCGGGCCTGGTGGCG
CTGATCGTCAAGAGCGCGCTGGGCCTGGAGCAAGCCGCCGGCGGCGTGGCCGGTTACGCGGTCAGCCAGGCGATGATGCT
GGGCATCAAGCGCGGCCTGTTCTCCAACGAGGCCGGCATGGGTTCGGCGCCCAACGCCGCCGCGACCGCCACCTCCAAGC
ATCCGGCCAGCCAGGGCGCGATCCAGATGTTCGGCGTGTTCATCGACACCATGGTGGTGTGCACCGCCACCGCGGCCATC
ATCCTGCTGTCCGGCGTGCACGACCAGGGACTGAGCGGCGTCGCGCTGACGCAGAAGGCCGTCGAATCGCAGTTCGGCCC
GATCGGCGTGTCCTTCCTGGCGGTGGCGATGTTCCTGTTCGCGTTCTCGTCCATCCTGGGCAATTACGCCTACGCCGAGG
GCAACGTGGAGTTCATCCGCAACAACAAGCGCGCCTTGCTGGGCTTCCGCCTGGTGGTGCTGGCCATGGTGATGTTCGGC
GCGCTGGCCAGCCTGCCGCTGGTATGGGACATGGCCGATCTGGCGATGGGCCTGATGGCGCTGATCAACCTGGTGGCCAT
CGTGCTGCTGTCGCGCTATTTCTTCACCGTGCTGGGCGATTACCAGCGCCAGCTGAAGGCCGGCGTCGCCGCGCCGGAAT
TCAAGGCCGACCAGTATCCGGAACTGGCCGGCAAGGTGGAGAAGGGCGTCTGGTAA

Upstream 100 bases:

>100_bases
TTGCGCCCGCTCGCAATGATTTCACCCGGCGTCAGACCGGGAGACCGTTCCGCGGCCGCGCGCCGTGGACTCCGATACGC
TCAGGACAACAAGGAAAACC

Downstream 100 bases:

>100_bases
GCCTGTCTCCCAATCCGCGATGACGAAGCCCGCCGTTTCCGGCGGGCTTTTTGTTTGGACGGCGCGGGCGAGCCCGGCGG
TCAGACGGTGAAGCGTCCGG

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MELLHSLINAVNDVLWGKVLIALLLAVGLYFSLRSGLLQLRLFSRGWREMMGGRGGHVSKNDISPFQAFATGLASRVGTG
NIAGVAIAIAAGGPGAVFWMWLTALLGMSSAFVESTLAQLFKTSHHDDTFRGGPAYYIQKGLGLRWLGVLFALCLILAFG
LVFNAVQANSIVAATEGAWGWNRYAVGVGLVLLTAPVIFGGVRSVARVAEWLVPVMALIYLLMAFYVLAIHAAELPGLVA
LIVKSALGLEQAAGGVAGYAVSQAMMLGIKRGLFSNEAGMGSAPNAAATATSKHPASQGAIQMFGVFIDTMVVCTATAAI
ILLSGVHDQGLSGVALTQKAVESQFGPIGVSFLAVAMFLFAFSSILGNYAYAEGNVEFIRNNKRALLGFRLVVLAMVMFG
ALASLPLVWDMADLAMGLMALINLVAIVLLSRYFFTVLGDYQRQLKAGVAAPEFKADQYPELAGKVEKGVW

Sequences:

>Translated_471_residues
MELLHSLINAVNDVLWGKVLIALLLAVGLYFSLRSGLLQLRLFSRGWREMMGGRGGHVSKNDISPFQAFATGLASRVGTG
NIAGVAIAIAAGGPGAVFWMWLTALLGMSSAFVESTLAQLFKTSHHDDTFRGGPAYYIQKGLGLRWLGVLFALCLILAFG
LVFNAVQANSIVAATEGAWGWNRYAVGVGLVLLTAPVIFGGVRSVARVAEWLVPVMALIYLLMAFYVLAIHAAELPGLVA
LIVKSALGLEQAAGGVAGYAVSQAMMLGIKRGLFSNEAGMGSAPNAAATATSKHPASQGAIQMFGVFIDTMVVCTATAAI
ILLSGVHDQGLSGVALTQKAVESQFGPIGVSFLAVAMFLFAFSSILGNYAYAEGNVEFIRNNKRALLGFRLVVLAMVMFG
ALASLPLVWDMADLAMGLMALINLVAIVLLSRYFFTVLGDYQRQLKAGVAAPEFKADQYPELAGKVEKGVW
>Mature_471_residues
MELLHSLINAVNDVLWGKVLIALLLAVGLYFSLRSGLLQLRLFSRGWREMMGGRGGHVSKNDISPFQAFATGLASRVGTG
NIAGVAIAIAAGGPGAVFWMWLTALLGMSSAFVESTLAQLFKTSHHDDTFRGGPAYYIQKGLGLRWLGVLFALCLILAFG
LVFNAVQANSIVAATEGAWGWNRYAVGVGLVLLTAPVIFGGVRSVARVAEWLVPVMALIYLLMAFYVLAIHAAELPGLVA
LIVKSALGLEQAAGGVAGYAVSQAMMLGIKRGLFSNEAGMGSAPNAAATATSKHPASQGAIQMFGVFIDTMVVCTATAAI
ILLSGVHDQGLSGVALTQKAVESQFGPIGVSFLAVAMFLFAFSSILGNYAYAEGNVEFIRNNKRALLGFRLVVLAMVMFG
ALASLPLVWDMADLAMGLMALINLVAIVLLSRYFFTVLGDYQRQLKAGVAAPEFKADQYPELAGKVEKGVW

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=466, Percent_Identity=44.206008583691, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49803; Mature: 49803

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLHSLINAVNDVLWGKVLIALLLAVGLYFSLRSGLLQLRLFSRGWREMMGGRGGHVSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDISPFQAFATGLASRVGTGNIAGVAIAIAAGGPGAVFWMWLTALLGMSSAFVESTLAQL
CCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
FKTSHHDDTFRGGPAYYIQKGLGLRWLGVLFALCLILAFGLVFNAVQANSIVAATEGAWG
HHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
WNRYAVGVGLVLLTAPVIFGGVRSVARVAEWLVPVMALIYLLMAFYVLAIHAAELPGLVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LIVKSALGLEQAAGGVAGYAVSQAMMLGIKRGLFSNEAGMGSAPNAAATATSKHPASQGA
HHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCHHH
IQMFGVFIDTMVVCTATAAIILLSGVHDQGLSGVALTQKAVESQFGPIGVSFLAVAMFLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
AFSSILGNYAYAEGNVEFIRNNKRALLGFRLVVLAMVMFGALASLPLVWDMADLAMGLMA
HHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LINLVAIVLLSRYFFTVLGDYQRQLKAGVAAPEFKADQYPELAGKVEKGVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MELLHSLINAVNDVLWGKVLIALLLAVGLYFSLRSGLLQLRLFSRGWREMMGGRGGHVSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDISPFQAFATGLASRVGTGNIAGVAIAIAAGGPGAVFWMWLTALLGMSSAFVESTLAQL
CCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
FKTSHHDDTFRGGPAYYIQKGLGLRWLGVLFALCLILAFGLVFNAVQANSIVAATEGAWG
HHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
WNRYAVGVGLVLLTAPVIFGGVRSVARVAEWLVPVMALIYLLMAFYVLAIHAAELPGLVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LIVKSALGLEQAAGGVAGYAVSQAMMLGIKRGLFSNEAGMGSAPNAAATATSKHPASQGA
HHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCHHH
IQMFGVFIDTMVVCTATAAIILLSGVHDQGLSGVALTQKAVESQFGPIGVSFLAVAMFLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
AFSSILGNYAYAEGNVEFIRNNKRALLGFRLVVLAMVMFGALASLPLVWDMADLAMGLMA
HHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LINLVAIVLLSRYFFTVLGDYQRQLKAGVAAPEFKADQYPELAGKVEKGVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]