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The map label for this gene is clcA [H]

Identifier: 34499074

GI number: 34499074

Start: 3924346

End: 3925713

Strand: Direct

Name: clcA [H]

Synonym: CV_3619

Alternate gene names: 34499074

Gene position: 3924346-3925713 (Clockwise)

Preceding gene: 34499073

Following gene: 34499075

Centisome position: 82.6

GC content: 68.49

Gene sequence:

>1368_bases
ATGCAGGATCACCTACCCCGCCGTTCCAGACGCCAGCGGATGAACCGCCGCCTGCGCCATCTCTACCTGTCCGCCGCCCA
GTTGCGGCGCCAGGTGCCGGTCTGGATCGCGGCCATCCTGATCGGCCTGGTGGCCTACGTGTTCGCGCTGGGCAGCCATT
GGTCGCACGACCTGTTTTTCAAGGCCTACGACGCGTCGCCGTACTGGAGCCTGCTGATCACGCCGGCCGGCTTGGCGCTG
GCGATCTGGATCATGCGGCGCTTTTTCCCCAGCTCCGCCGGCGGCGGCATTCCGCAGTCGATCGCCGCGCTGGAGCCCGG
CATGGAAAAGCTGCGCGAGTACTGCTTCACGCTGAAGGCCGCCTTCGGCAAGGTGCTGCTGACGCTGATCGGCATCAGCT
CCGGCGCCACCTTCGGCTATGAAGGCCCCATCGTCCAGGTCGGCGCGGCGATCAAGTATTCGCTGAACCGCAAGGCGGCG
CAGCGCCACGAGGGCGCCGCCCGCAGCTTCATCCTGGCCGGCGGCGCCGCCGGCGTGGCCGCGGCCTTCAACGCGCCGCT
GGCCGGCATCGTGTTCGCCATCGAGGAAATGGGCCGCATCTTCGACATGCGCGCCAGCTCCACCATCCTGCTGTCGGTGA
TCGTCGCCGGCCTCACCACCTTCGCCATCAACGGCAACTACAGCTACTTCGGCATCGTCCACGTGGCGATGGACAACCAG
CAGGCCTGGCTGGCCATCCCGGCCTGCGGCGTCGTCGGCGGACTGATAGGCGGGCTGGCCTGCCGCGTGCTGCTGGCCTT
GTCCAGCCGGCTGCCCGGCCCGCTGCACGGCTGGCGCATCCGCTATCCGATCCGCTTCGCCGCCGGCTGCGGCCTGGCGC
TGGCCGTGATCGGCATCGTCAGCCACGGCAGCACCTTCGGCACCGGCTATTTCCGCGCCCGCGACATCATCGAGGGCCAC
GGCGCGGCGCTGCAGGATTACGGCGTGCTGAAGCTGATCAGCATGTTCGTCAGCCACATCAGCGCGGTGCCGGGCGGCAT
GTTCGCGCCATCGCTGGCTGTCGGCGCCGGCTTCGGCCTCAATATGTCGCAGCTGCTGCCCTTCCTGCCGCAGAGCATGG
TGGTGCTGCTGGGCATGGTGGGCTTTTTCGCCGGCATGACGCGCGCGCCGATGACAGGCTTCGTCATCGTGATGGAGATG
ACCGACACCTCGGGCATGATCATTCCGCTGATGGCCACCGCGCTGATCGCGGCCTCGGTGTCCCGGCTGGTGAGCCGGCG
CGCGCTGTACGACGGCCAAGTGCGGCTGTTCCTGCCGCAATGGCGGACCCAGGCCGGCCAAATTGACCAGCCAGCCGCCC
GCACGTAG

Upstream 100 bases:

>100_bases
GCAGGCGGGCCGCCCTCGTATTAGGCGGCAATAGAGAGTATATTAGACAAAAATGATTAATGGGCGGAATTGGACTCCGC
CCAATGGGAGCCGCCAAACG

Downstream 100 bases:

>100_bases
AATAACCTATTGAAAAGATCGGGTATTTCCGCCGGCTCCTTCGCCGGCCCACAGCAAGGATTCAATACATGTCGATTCAA
CAAGATATCCAGCAGACCGT

Product: voltage-gated chloride channel family protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MQDHLPRRSRRQRMNRRLRHLYLSAAQLRRQVPVWIAAILIGLVAYVFALGSHWSHDLFFKAYDASPYWSLLITPAGLAL
AIWIMRRFFPSSAGGGIPQSIAALEPGMEKLREYCFTLKAAFGKVLLTLIGISSGATFGYEGPIVQVGAAIKYSLNRKAA
QRHEGAARSFILAGGAAGVAAAFNAPLAGIVFAIEEMGRIFDMRASSTILLSVIVAGLTTFAINGNYSYFGIVHVAMDNQ
QAWLAIPACGVVGGLIGGLACRVLLALSSRLPGPLHGWRIRYPIRFAAGCGLALAVIGIVSHGSTFGTGYFRARDIIEGH
GAALQDYGVLKLISMFVSHISAVPGGMFAPSLAVGAGFGLNMSQLLPFLPQSMVVLLGMVGFFAGMTRAPMTGFVIVMEM
TDTSGMIIPLMATALIAASVSRLVSRRALYDGQVRLFLPQWRTQAGQIDQPAART

Sequences:

>Translated_455_residues
MQDHLPRRSRRQRMNRRLRHLYLSAAQLRRQVPVWIAAILIGLVAYVFALGSHWSHDLFFKAYDASPYWSLLITPAGLAL
AIWIMRRFFPSSAGGGIPQSIAALEPGMEKLREYCFTLKAAFGKVLLTLIGISSGATFGYEGPIVQVGAAIKYSLNRKAA
QRHEGAARSFILAGGAAGVAAAFNAPLAGIVFAIEEMGRIFDMRASSTILLSVIVAGLTTFAINGNYSYFGIVHVAMDNQ
QAWLAIPACGVVGGLIGGLACRVLLALSSRLPGPLHGWRIRYPIRFAAGCGLALAVIGIVSHGSTFGTGYFRARDIIEGH
GAALQDYGVLKLISMFVSHISAVPGGMFAPSLAVGAGFGLNMSQLLPFLPQSMVVLLGMVGFFAGMTRAPMTGFVIVMEM
TDTSGMIIPLMATALIAASVSRLVSRRALYDGQVRLFLPQWRTQAGQIDQPAART
>Mature_455_residues
MQDHLPRRSRRQRMNRRLRHLYLSAAQLRRQVPVWIAAILIGLVAYVFALGSHWSHDLFFKAYDASPYWSLLITPAGLAL
AIWIMRRFFPSSAGGGIPQSIAALEPGMEKLREYCFTLKAAFGKVLLTLIGISSGATFGYEGPIVQVGAAIKYSLNRKAA
QRHEGAARSFILAGGAAGVAAAFNAPLAGIVFAIEEMGRIFDMRASSTILLSVIVAGLTTFAINGNYSYFGIVHVAMDNQ
QAWLAIPACGVVGGLIGGLACRVLLALSSRLPGPLHGWRIRYPIRFAAGCGLALAVIGIVSHGSTFGTGYFRARDIIEGH
GAALQDYGVLKLISMFVSHISAVPGGMFAPSLAVGAGFGLNMSQLLPFLPQSMVVLLGMVGFFAGMTRAPMTGFVIVMEM
TDTSGMIIPLMATALIAASVSRLVSRRALYDGQVRLFLPQWRTQAGQIDQPAART

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557473, Length=441, Percent_Identity=25.6235827664399, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI189217923, Length=441, Percent_Identity=25.6235827664399, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI189217921, Length=441, Percent_Identity=25.6235827664399, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI153252026, Length=464, Percent_Identity=24.1379310344828, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI55770840, Length=435, Percent_Identity=23.6781609195402, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI55770838, Length=435, Percent_Identity=23.6781609195402, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786350, Length=442, Percent_Identity=27.6018099547511, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081943, Length=333, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 48775; Mature: 48775

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDHLPRRSRRQRMNRRLRHLYLSAAQLRRQVPVWIAAILIGLVAYVFALGSHWSHDLFF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
KAYDASPYWSLLITPAGLALAIWIMRRFFPSSAGGGIPQSIAALEPGMEKLREYCFTLKA
EEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
AFGKVLLTLIGISSGATFGYEGPIVQVGAAIKYSLNRKAAQRHEGAARSFILAGGAAGVA
HHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHEEEECCCHHHH
AAFNAPLAGIVFAIEEMGRIFDMRASSTILLSVIVAGLTTFAINGNYSYFGIVHVAMDNQ
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEEEEECCC
QAWLAIPACGVVGGLIGGLACRVLLALSSRLPGPLHGWRIRYPIRFAAGCGLALAVIGIV
CEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECEEECCCCHHHHHHHHHH
SHGSTFGTGYFRARDIIEGHGAALQDYGVLKLISMFVSHISAVPGGMFAPSLAVGAGFGL
HCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCC
NMSQLLPFLPQSMVVLLGMVGFFAGMTRAPMTGFVIVMEMTDTSGMIIPLMATALIAASV
CHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHH
SRLVSRRALYDGQVRLFLPQWRTQAGQIDQPAART
HHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQDHLPRRSRRQRMNRRLRHLYLSAAQLRRQVPVWIAAILIGLVAYVFALGSHWSHDLFF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
KAYDASPYWSLLITPAGLALAIWIMRRFFPSSAGGGIPQSIAALEPGMEKLREYCFTLKA
EEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
AFGKVLLTLIGISSGATFGYEGPIVQVGAAIKYSLNRKAAQRHEGAARSFILAGGAAGVA
HHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHEEEECCCHHHH
AAFNAPLAGIVFAIEEMGRIFDMRASSTILLSVIVAGLTTFAINGNYSYFGIVHVAMDNQ
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEEEEECCC
QAWLAIPACGVVGGLIGGLACRVLLALSSRLPGPLHGWRIRYPIRFAAGCGLALAVIGIV
CEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECEEECCCCHHHHHHHHHH
SHGSTFGTGYFRARDIIEGHGAALQDYGVLKLISMFVSHISAVPGGMFAPSLAVGAGFGL
HCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCC
NMSQLLPFLPQSMVVLLGMVGFFAGMTRAPMTGFVIVMEMTDTSGMIIPLMATALIAASV
CHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHH
SRLVSRRALYDGQVRLFLPQWRTQAGQIDQPAART
HHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA