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The map label for this gene is purA2

Identifier: 34498983

GI number: 34498983

Start: 3835680

End: 3836975

Strand: Reverse

Name: purA2

Synonym: CV_3528

Alternate gene names: 34498983

Gene position: 3836975-3835680 (Counterclockwise)

Preceding gene: 34498984

Following gene: 34498980

Centisome position: 80.76

GC content: 65.35

Gene sequence:

>1296_bases
ATGTCCAAGAACGTTGTCGTGATTGGCACCCAGTGGGGTGACGAAGGCAAAGGCAAAATCGTTGACTGGCTGACCGACCA
CGCGCGTGCCGTTGTGCGCTTCCAAGGCGGCCACAACGCCGGCCACACCCTGTGGGTGAATGGCAAGAAAACCGTCGTGC
GCCTGGTGCCGTCCGGCATTCTGCGCCCGGATGTGGAGTGCTTCATCGGCAACGGCGTGGTGCTGTCGCCGGAAGCGCTG
CTGAAGGAGATCGACGAGCTGGAGGCCGCCGGCGTCAACGCCTCCGCCCGCCTGAAGATCGCCGAAGGCTGCCCGCTGAT
CCTGCCGTACCACATCGCGCTGGACCAGGCCCGCGAAGCCGCCAAGGGCGACGCCAAGATCGGCACCACCGGCCGCGGCA
TCGGCCCCTGCTACGAAGACAAGGTGGCCCGCCGCGCGCTGAAGGTGATCGACCTGTTCGATCCGGCCCGCTTCGAGACC
AAGCTGAAGGAAAACGTAGACTACTACAACTTCCTGCTGACCAATCTGTTCAAGGCCGAGCCGGTGAGCTATGAAGCGAT
CCTGGCCGACACCATGAAGATGGCCGAGCGCATCAAGCCGATGGTGGCCGACGTGTCCCGCACCCTGTACGACCTGGACA
AGGCCGGCACGCCCATTCTGTTCGAAGGCGCGCAGGGTACGCTGCTGGACATCGACCACGGCACTTACCCGTATGTCACC
TCGTCCAACTGCGTGGCCGGCGCCGCCGCGCCGGGCGCCGGCGTGCCGCCGCAAATGCTGAACTACGTGCTGGGCATCGT
GAAGGGCTACGCCACCCGCGTCGGCTCCGGTCCGTTCCCGACCGAGCAGGAAAACGAAATCGGCGCCTTCCTGGCCAAGC
GCGGCAACGAGTTCGGCTCCGTCACCGGTCGTCCGCGCCGCTGCGGCTGGTTCGACGCCGCCGCGCTGAAGCGCTCGATC
CAGATCAACGGCGTGTCCGGCCTGTGCGTGATGAAGCTGGACGTGATGGACGGCCTGGAAGAGATCAAGCTGTGCACCGG
CTACATGCTGGATGGCCAGAAGGTCGACATCTTGCCGTTCGGTTCCGATGCCGTCACCAAGTGCGAGCCGGTGTACGAAA
CCCTGCCGGGCTGGACCGGCACCACCGTCGGCGTCAAGCGCTGGGAAGACCTGCCGGCCAACGCCCAGGCTTACCTGAAG
CGCATCGAAGAAGTTTGCGGCGCGCCGGTCGACATCGTGTCCACCGGTCCGGACCGCGAAGAAACCATCGTGCTGCGCCA
CCCGTTCGGCCTGTAA

Upstream 100 bases:

>100_bases
CAATTGCGACCGAGAGTTGGTTCCCGCTGCCGATGGTTGGCAGCTGGCGCCGTTTCATTGAATTTTTGTTTGAATCTGAC
TGACGAAGAGGTTTTTTCCA

Downstream 100 bases:

>100_bases
GCCGAGCGCTGGTTCCCGCTGTTGGAAAGCCCACCGCTTGCGGTGGGCTTTTTGTTTGCCTGCTGGGGCCTGCTCATGAA
TGGCTTCCGGCGCCGGACGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSKNVVVIGTQWGDEGKGKIVDWLTDHARAVVRFQGGHNAGHTLWVNGKKTVVRLVPSGILRPDVECFIGNGVVLSPEAL
LKEIDELEAAGVNASARLKIAEGCPLILPYHIALDQAREAAKGDAKIGTTGRGIGPCYEDKVARRALKVIDLFDPARFET
KLKENVDYYNFLLTNLFKAEPVSYEAILADTMKMAERIKPMVADVSRTLYDLDKAGTPILFEGAQGTLLDIDHGTYPYVT
SSNCVAGAAAPGAGVPPQMLNYVLGIVKGYATRVGSGPFPTEQENEIGAFLAKRGNEFGSVTGRPRRCGWFDAAALKRSI
QINGVSGLCVMKLDVMDGLEEIKLCTGYMLDGQKVDILPFGSDAVTKCEPVYETLPGWTGTTVGVKRWEDLPANAQAYLK
RIEEVCGAPVDIVSTGPDREETIVLRHPFGL

Sequences:

>Translated_431_residues
MSKNVVVIGTQWGDEGKGKIVDWLTDHARAVVRFQGGHNAGHTLWVNGKKTVVRLVPSGILRPDVECFIGNGVVLSPEAL
LKEIDELEAAGVNASARLKIAEGCPLILPYHIALDQAREAAKGDAKIGTTGRGIGPCYEDKVARRALKVIDLFDPARFET
KLKENVDYYNFLLTNLFKAEPVSYEAILADTMKMAERIKPMVADVSRTLYDLDKAGTPILFEGAQGTLLDIDHGTYPYVT
SSNCVAGAAAPGAGVPPQMLNYVLGIVKGYATRVGSGPFPTEQENEIGAFLAKRGNEFGSVTGRPRRCGWFDAAALKRSI
QINGVSGLCVMKLDVMDGLEEIKLCTGYMLDGQKVDILPFGSDAVTKCEPVYETLPGWTGTTVGVKRWEDLPANAQAYLK
RIEEVCGAPVDIVSTGPDREETIVLRHPFGL
>Mature_430_residues
SKNVVVIGTQWGDEGKGKIVDWLTDHARAVVRFQGGHNAGHTLWVNGKKTVVRLVPSGILRPDVECFIGNGVVLSPEALL
KEIDELEAAGVNASARLKIAEGCPLILPYHIALDQAREAAKGDAKIGTTGRGIGPCYEDKVARRALKVIDLFDPARFETK
LKENVDYYNFLLTNLFKAEPVSYEAILADTMKMAERIKPMVADVSRTLYDLDKAGTPILFEGAQGTLLDIDHGTYPYVTS
SNCVAGAAAPGAGVPPQMLNYVLGIVKGYATRVGSGPFPTEQENEIGAFLAKRGNEFGSVTGRPRRCGWFDAAALKRSIQ
INGVSGLCVMKLDVMDGLEEIKLCTGYMLDGQKVDILPFGSDAVTKCEPVYETLPGWTGTTVGVKRWEDLPANAQAYLKR
IEEVCGAPVDIVSTGPDREETIVLRHPFGL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=43.7209302325581, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=42.3611111111111, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=42.5692695214106, Blast_Score=308, Evalue=7e-84,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=60.4651162790698, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=41.3394919168591, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=41.3394919168591, Blast_Score=308, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=43.5897435897436, Blast_Score=343, Evalue=3e-95,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.8235294117647, Blast_Score=324, Evalue=8e-89,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA2_CHRVO (Q7NS98)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903198.1
- ProteinModelPortal:   Q7NS98
- SMR:   Q7NS98
- GeneID:   2547652
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3528
- NMPDR:   fig|243365.1.peg.3528
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q7NS98
- ProtClustDB:   PRK01117
- BioCyc:   CVIO243365:CV_3528-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46638; Mature: 46507

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNVVVIGTQWGDEGKGKIVDWLTDHARAVVRFQGGHNAGHTLWVNGKKTVVRLVPSGI
CCCCEEEEEECCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCC
LRPDVECFIGNGVVLSPEALLKEIDELEAAGVNASARLKIAEGCPLILPYHIALDQAREA
CCCCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHH
AKGDAKIGTTGRGIGPCYEDKVARRALKVIDLFDPARFETKLKENVDYYNFLLTNLFKAE
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
PVSYEAILADTMKMAERIKPMVADVSRTLYDLDKAGTPILFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEEE
SSNCVAGAAAPGAGVPPQMLNYVLGIVKGYATRVGSGPFPTEQENEIGAFLAKRGNEFGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
VTGRPRRCGWFDAAALKRSIQINGVSGLCVMKLDVMDGLEEIKLCTGYMLDGQKVDILPF
CCCCCCCCCCHHHHHHHCEEEECCCCCEEEEEEHHHCCHHHHHHHHCEEECCCEEEEEEC
GSDAVTKCEPVYETLPGWTGTTVGVKRWEDLPANAQAYLKRIEEVCGAPVDIVSTGPDRE
CCCCCHHCCHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
ETIVLRHPFGL
CEEEEECCCCC
>Mature Secondary Structure 
SKNVVVIGTQWGDEGKGKIVDWLTDHARAVVRFQGGHNAGHTLWVNGKKTVVRLVPSGI
CCCEEEEEECCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCC
LRPDVECFIGNGVVLSPEALLKEIDELEAAGVNASARLKIAEGCPLILPYHIALDQAREA
CCCCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHH
AKGDAKIGTTGRGIGPCYEDKVARRALKVIDLFDPARFETKLKENVDYYNFLLTNLFKAE
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
PVSYEAILADTMKMAERIKPMVADVSRTLYDLDKAGTPILFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEEE
SSNCVAGAAAPGAGVPPQMLNYVLGIVKGYATRVGSGPFPTEQENEIGAFLAKRGNEFGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
VTGRPRRCGWFDAAALKRSIQINGVSGLCVMKLDVMDGLEEIKLCTGYMLDGQKVDILPF
CCCCCCCCCCHHHHHHHCEEEECCCCCEEEEEEHHHCCHHHHHHHHCEEECCCEEEEEEC
GSDAVTKCEPVYETLPGWTGTTVGVKRWEDLPANAQAYLKRIEEVCGAPVDIVSTGPDRE
CCCCCHHCCHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
ETIVLRHPFGL
CEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782