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The map label for this gene is kdsB

Identifier: 34498799

GI number: 34498799

Start: 3642200

End: 3642955

Strand: Reverse

Name: kdsB

Synonym: CV_3344

Alternate gene names: 34498799

Gene position: 3642955-3642200 (Counterclockwise)

Preceding gene: 34498800

Following gene: 34498798

Centisome position: 76.68

GC content: 70.77

Gene sequence:

>756_bases
ATGAACGGCTTCACGGTGGTGATCCCGGCGCGGATGGCGTCCAGCCGTCTGCCTGGCAAGCCGCTGGCAGACATCGCCGG
CAAGCCCATGGTGGTGCGCGTGGCGGAGCAGGCGGCGAAGAGCCGCGCCGCCCGCGTCGTGGTCGCCACCGACCACGCCG
ACATCCTGGCCGCCTGCGCCGCGCACGGCGTCGAGGCGGTGCTGACGCGCGAAGACCACGCCAGCGGCACCGATCGCCTA
GCCGAAGTGGCCGCCAAGCTGGCGTTGCCGGACGACGCGCTGGTGGTCAACGTGCAGGGCGACGAGCCGCTGATCCAGCC
TGAGCTGATCAACCGGCTGGCCGAGCTGCTGGCCGGCGCCGACGCGCCGGTGGCGACCCTGGCGCACGCGCTGCACGACG
CGGCCGACCATTTCAATCCCAATGTGGTGAAAGTGGCGCTGGACAAGCATGGCCGCGCGCTGTACTTCAGCCGCGCGCCG
ATTCCCTACGCCCGCGACGCCTACGCCGCCGACCGCTCGGCGTTGCCGGCCGGACTGCCGGTTTACCGCCATATCGGCAT
GTACGGTTACCGGGCCGGTTTCCTGGCTGCCTATGCAGGCCTGGAGCCGGGGCCGCTGGAGCAATACGAAGCGCTGGAAC
AATTGCGCGTGCTCTGGCATGGTTACGGCATCGCGGTGGCATTGGCGGACGAGGCGCCGGCCGCCGGCGTGGACACGCCG
GAAGACCTGGAACGGGTGCGCCGGCTGTTCGCCTGA

Upstream 100 bases:

>100_bases
TGATCTGCAAGGGAGACCGCCTGGCGTTCCCGATCAAGGACGGCATCCCGATGATGCTGGAGAGCGAAGCGCGCGAGCTG
GCCCCGGAAGAAGAAGTCAA

Downstream 100 bases:

>100_bases
GGGGCAGGGAGCGGCCGCTCCGTCAAAGAATTCATAAATCAAACAGAACTGGAGTCACACAAATGCGATTGATCCTGTTG
GGCGCGCCCGGCGCCGGCAA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNGFTVVIPARMASSRLPGKPLADIAGKPMVVRVAEQAAKSRAARVVVATDHADILAACAAHGVEAVLTREDHASGTDRL
AEVAAKLALPDDALVVNVQGDEPLIQPELINRLAELLAGADAPVATLAHALHDAADHFNPNVVKVALDKHGRALYFSRAP
IPYARDAYAADRSALPAGLPVYRHIGMYGYRAGFLAAYAGLEPGPLEQYEALEQLRVLWHGYGIAVALADEAPAAGVDTP
EDLERVRRLFA

Sequences:

>Translated_251_residues
MNGFTVVIPARMASSRLPGKPLADIAGKPMVVRVAEQAAKSRAARVVVATDHADILAACAAHGVEAVLTREDHASGTDRL
AEVAAKLALPDDALVVNVQGDEPLIQPELINRLAELLAGADAPVATLAHALHDAADHFNPNVVKVALDKHGRALYFSRAP
IPYARDAYAADRSALPAGLPVYRHIGMYGYRAGFLAAYAGLEPGPLEQYEALEQLRVLWHGYGIAVALADEAPAAGVDTP
EDLERVRRLFA
>Mature_251_residues
MNGFTVVIPARMASSRLPGKPLADIAGKPMVVRVAEQAAKSRAARVVVATDHADILAACAAHGVEAVLTREDHASGTDRL
AEVAAKLALPDDALVVNVQGDEPLIQPELINRLAELLAGADAPVATLAHALHDAADHFNPNVVKVALDKHGRALYFSRAP
IPYARDAYAADRSALPAGLPVYRHIGMYGYRAGFLAAYAGLEPGPLEQYEALEQLRVLWHGYGIAVALADEAPAAGVDTP
EDLERVRRLFA

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=244, Percent_Identity=56.9672131147541, Blast_Score=273, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_CHRVO (Q7NSS6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903014.1
- HSSP:   P04951
- ProteinModelPortal:   Q7NSS6
- SMR:   Q7NSS6
- GeneID:   2547822
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3344
- NMPDR:   fig|243365.1.peg.3344
- HOGENOM:   HBG637773
- OMA:   FSRAPLP
- PhylomeDB:   Q7NSS6
- ProtClustDB:   PRK05450
- BioCyc:   CVIO243365:CV_3344-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 26627; Mature: 26627

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFTVVIPARMASSRLPGKPLADIAGKPMVVRVAEQAAKSRAARVVVATDHADILAACA
CCCEEEEEECHHHHCCCCCCCHHHHCCCCEEEHHHHHHHHHHHEEEEEEECCHHHHHHHH
AHGVEAVLTREDHASGTDRLAEVAAKLALPDDALVVNVQGDEPLIQPELINRLAELLAGA
HHCHHEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
DAPVATLAHALHDAADHFNPNVVKVALDKHGRALYFSRAPIPYARDAYAADRSALPAGLP
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCH
VYRHIGMYGYRAGFLAAYAGLEPGPLEQYEALEQLRVLWHGYGIAVALADEAPAAGVDTP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCH
EDLERVRRLFA
HHHHHHHHHHC
>Mature Secondary Structure
MNGFTVVIPARMASSRLPGKPLADIAGKPMVVRVAEQAAKSRAARVVVATDHADILAACA
CCCEEEEEECHHHHCCCCCCCHHHHCCCCEEEHHHHHHHHHHHEEEEEEECCHHHHHHHH
AHGVEAVLTREDHASGTDRLAEVAAKLALPDDALVVNVQGDEPLIQPELINRLAELLAGA
HHCHHEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
DAPVATLAHALHDAADHFNPNVVKVALDKHGRALYFSRAPIPYARDAYAADRSALPAGLP
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCH
VYRHIGMYGYRAGFLAAYAGLEPGPLEQYEALEQLRVLWHGYGIAVALADEAPAAGVDTP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCH
EDLERVRRLFA
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782