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The map label for this gene is lipB

Identifier: 34498551

GI number: 34498551

Start: 3381029

End: 3381652

Strand: Direct

Name: lipB

Synonym: CV_3096

Alternate gene names: 34498551

Gene position: 3381029-3381652 (Clockwise)

Preceding gene: 34498550

Following gene: 34498552

Centisome position: 71.16

GC content: 64.42

Gene sequence:

>624_bases
GTGCCTCGCATCATCAAACATCTGGGCCGGGTCGATTACGAACCGACCTGGCGCGCCATGCAAGCCTTCACCGACAGCCG
CGGCGCCGAAACCCGCGACGAGCTGTGGGTAGTGGAGCATCCTCCGGTGTTCACCCAGGGCCTGGCCGGCAAGCCGGAAC
ACCTGCTGCAGCAGAACGACGTCCCGGTGGTGAAAACCGACCGCGGCGGCCAGATCACCTACCACGGCCCCGGCCAGCTG
GTGGTCTACCTGCTGGTGGACTTCAAACGCATGCATGTCGGCGTGCGCGAGCTGGTGCGCCGCATCGAGCAGGCCATCAT
AGACATGCTGGCCGAACAGGGCATCGCCGCCAACGGCGACGTCGATGCGCCTGGGGTATACGTGGACGGCGCCAAGATCG
CCTCGCTGGGCCTGCGCATCAAGAACGGCGCCACCTACCACGGCCTGAGCCTCAATGTGGACATGGACCTGACCCCGTTT
AGCTGGATCAACCCTTGCGGCTACGCCAACCTCAAGGTGACCCAAATGAAGAATCTGGGCGTGAACCTCACGGTCGCCGA
AGCCGCCGACAAGCTGCTGCCGCACCTGGAACGCCACCTGAGCACAAGCAAGGAGACCGCATGA

Upstream 100 bases:

>100_bases
ACCGTCACCGCCACCTCGCGCCAGCAGCTGGACAACATCTATCTGTCGCTGACCGGCCATCCGATGGTAAAAATGGTCCT
CTAAAACACCGGGAGTTCAG

Downstream 100 bases:

>100_bases
AGCCGGACAACCAGGCCGGGGTGAAGCACAAGGGCGCGGCCAAGACCGCCCGCATCCCGATCAAGATCGTGCCGCTGGAC
GAAAAGCTGAAAAAGCCGGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPRIIKHLGRVDYEPTWRAMQAFTDSRGAETRDELWVVEHPPVFTQGLAGKPEHLLQQNDVPVVKTDRGGQITYHGPGQL
VVYLLVDFKRMHVGVRELVRRIEQAIIDMLAEQGIAANGDVDAPGVYVDGAKIASLGLRIKNGATYHGLSLNVDMDLTPF
SWINPCGYANLKVTQMKNLGVNLTVAEAADKLLPHLERHLSTSKETA

Sequences:

>Translated_207_residues
MPRIIKHLGRVDYEPTWRAMQAFTDSRGAETRDELWVVEHPPVFTQGLAGKPEHLLQQNDVPVVKTDRGGQITYHGPGQL
VVYLLVDFKRMHVGVRELVRRIEQAIIDMLAEQGIAANGDVDAPGVYVDGAKIASLGLRIKNGATYHGLSLNVDMDLTPF
SWINPCGYANLKVTQMKNLGVNLTVAEAADKLLPHLERHLSTSKETA
>Mature_206_residues
PRIIKHLGRVDYEPTWRAMQAFTDSRGAETRDELWVVEHPPVFTQGLAGKPEHLLQQNDVPVVKTDRGGQITYHGPGQLV
VYLLVDFKRMHVGVRELVRRIEQAIIDMLAEQGIAANGDVDAPGVYVDGAKIASLGLRIKNGATYHGLSLNVDMDLTPFS
WINPCGYANLKVTQMKNLGVNLTVAEAADKLLPHLERHLSTSKETA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=208, Percent_Identity=34.1346153846154, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI87081767, Length=193, Percent_Identity=52.8497409326425, Blast_Score=219, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323268, Length=136, Percent_Identity=34.5588235294118, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=171, Percent_Identity=35.0877192982456, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_CHRVO (Q7NTG0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902766.1
- ProteinModelPortal:   Q7NTG0
- SMR:   Q7NTG0
- GeneID:   2548563
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3096
- NMPDR:   fig|243365.1.peg.3096
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q7NTG0
- ProtClustDB:   PRK14342
- BioCyc:   CVIO243365:CV_3096-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 22914; Mature: 22782

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIIKHLGRVDYEPTWRAMQAFTDSRGAETRDELWVVEHPPVFTQGLAGKPEHLLQQND
CCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHCCC
VPVVKTDRGGQITYHGPGQLVVYLLVDFKRMHVGVRELVRRIEQAIIDMLAEQGIAANGD
CCEEEECCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VDAPGVYVDGAKIASLGLRIKNGATYHGLSLNVDMDLTPFSWINPCGYANLKVTQMKNLG
CCCCCEEECCCEEEEECEEECCCCEEECEEEEECCCCCCCCCCCCCCCCCEEEEEECCCC
VNLTVAEAADKLLPHLERHLSTSKETA
CEEEHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PRIIKHLGRVDYEPTWRAMQAFTDSRGAETRDELWVVEHPPVFTQGLAGKPEHLLQQND
CHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHCCC
VPVVKTDRGGQITYHGPGQLVVYLLVDFKRMHVGVRELVRRIEQAIIDMLAEQGIAANGD
CCEEEECCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VDAPGVYVDGAKIASLGLRIKNGATYHGLSLNVDMDLTPFSWINPCGYANLKVTQMKNLG
CCCCCEEECCCEEEEECEEECCCCEEECEEEEECCCCCCCCCCCCCCCCCEEEEEECCCC
VNLTVAEAADKLLPHLERHLSTSKETA
CEEEHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782