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The map label for this gene is lipA

Identifier: 34498552

GI number: 34498552

Start: 3381649

End: 3382596

Strand: Direct

Name: lipA

Synonym: CV_3097

Alternate gene names: 34498552

Gene position: 3381649-3382596 (Clockwise)

Preceding gene: 34498551

Following gene: 34498553

Centisome position: 71.18

GC content: 64.77

Gene sequence:

>948_bases
ATGAAGCCGGACAACCAGGCCGGGGTGAAGCACAAGGGCGCGGCCAAGACCGCCCGCATCCCGATCAAGATCGTGCCGCT
GGACGAAAAGCTGAAAAAGCCGGAGTGGATCCGCGCCAAGCTGCCCAATGGCCAGCGTTTCCACGAGATCAAGCAGATCC
TGCGCGAGCAGAAGCTGCACACGGTCTGCGAAGAGGCCACCTGCCCGAACATCGGCGAGTGCTTCAGCAAGGGCACCGCC
ACCTTTATGATCATGGGCGACATCTGCACCCGCCGCTGCCCGTTCTGCGACGTCGGCCACGGCCGTCCCAATCCGCTGGA
CGAGAACGAGCCGAGACACCTGGCCGAGAGCGTGGCCGCGATGCGCCTGAAGTACGTGGTGGTCACCTCGGTGGACCGCG
ACGACCTGCGCGACGGCGGCGCCCAACACTTCGCAGACTGCATCAACGCCGTGCGCGAAATGTCGCCGGCGACCCAGATC
GAGACCCTGGTGCCCGACTTCCGCGGCCGGCTGGACATCGCCGTCGACATCCTGACCCAGACGCCGCCGGACGTGATGAA
CCACAACCTGGAAACCGTGCCGCGCCTGTACAAGCAGGCGCGCCCGGGCGCCGACTACGCGCACTCGCTGCAGCTCCTCA
AGGACTACAAGGCGAAGAATCCGAACGTGCGCACCAAGTCCGGCCTGATGGTGGGCTTGGGCGAGACCGACGAGGAAATC
CTGGAAGTGATGCGCGACCTGCGCGCGCACAACGTGGACATGCTGACCATAGGCCAGTACCTGCAGCCGTCCGACGGCCA
CCTGCCGGTGCTGCGCTACGTGCATCCCGACGTATTCAAGATGTTCGAGGAAAAAGCCTATGAAATGGGCTTCGTCCATG
CCGCGGTCGGCGCGATGGTGCGCTCCAGCTACCATGCCGACGTGCAGGCGCACGAAGCCGGCGTTTAA

Upstream 100 bases:

>100_bases
CCCAAATGAAGAATCTGGGCGTGAACCTCACGGTCGCCGAAGCCGCCGACAAGCTGCTGCCGCACCTGGAACGCCACCTG
AGCACAAGCAAGGAGACCGC

Downstream 100 bases:

>100_bases
TTTCCGCAACTCACTCAAACGGCATCCAGGGCGCCAATCCGGATGCCGTTTTTCTATCCTCTCGCCGTTTCTTTCATCCC
GCCTCATGAATATCCTGCGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKPDNQAGVKHKGAAKTARIPIKIVPLDEKLKKPEWIRAKLPNGQRFHEIKQILREQKLHTVCEEATCPNIGECFSKGTA
TFMIMGDICTRRCPFCDVGHGRPNPLDENEPRHLAESVAAMRLKYVVVTSVDRDDLRDGGAQHFADCINAVREMSPATQI
ETLVPDFRGRLDIAVDILTQTPPDVMNHNLETVPRLYKQARPGADYAHSLQLLKDYKAKNPNVRTKSGLMVGLGETDEEI
LEVMRDLRAHNVDMLTIGQYLQPSDGHLPVLRYVHPDVFKMFEEKAYEMGFVHAAVGAMVRSSYHADVQAHEAGV

Sequences:

>Translated_315_residues
MKPDNQAGVKHKGAAKTARIPIKIVPLDEKLKKPEWIRAKLPNGQRFHEIKQILREQKLHTVCEEATCPNIGECFSKGTA
TFMIMGDICTRRCPFCDVGHGRPNPLDENEPRHLAESVAAMRLKYVVVTSVDRDDLRDGGAQHFADCINAVREMSPATQI
ETLVPDFRGRLDIAVDILTQTPPDVMNHNLETVPRLYKQARPGADYAHSLQLLKDYKAKNPNVRTKSGLMVGLGETDEEI
LEVMRDLRAHNVDMLTIGQYLQPSDGHLPVLRYVHPDVFKMFEEKAYEMGFVHAAVGAMVRSSYHADVQAHEAGV
>Mature_315_residues
MKPDNQAGVKHKGAAKTARIPIKIVPLDEKLKKPEWIRAKLPNGQRFHEIKQILREQKLHTVCEEATCPNIGECFSKGTA
TFMIMGDICTRRCPFCDVGHGRPNPLDENEPRHLAESVAAMRLKYVVVTSVDRDDLRDGGAQHFADCINAVREMSPATQI
ETLVPDFRGRLDIAVDILTQTPPDVMNHNLETVPRLYKQARPGADYAHSLQLLKDYKAKNPNVRTKSGLMVGLGETDEEI
LEVMRDLRAHNVDMLTIGQYLQPSDGHLPVLRYVHPDVFKMFEEKAYEMGFVHAAVGAMVRSSYHADVQAHEAGV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=47.0175438596491, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=47.3895582329317, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=308, Percent_Identity=65.2597402597403, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32564533, Length=295, Percent_Identity=43.3898305084746, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=46.8531468531469, Blast_Score=265, Evalue=5e-72,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=48.2517482517483, Blast_Score=277, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CHRVO (Q7NTF9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902767.1
- ProteinModelPortal:   Q7NTF9
- GeneID:   2548564
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3097
- NMPDR:   fig|243365.1.peg.3097
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q7NTF9
- ProtClustDB:   PRK05481
- BioCyc:   CVIO243365:CV_3097-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35337; Mature: 35337

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDNQAGVKHKGAAKTARIPIKIVPLDEKLKKPEWIRAKLPNGQRFHEIKQILREQKLH
CCCCCCCCCCCCCCCCEECCCEEEECCHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
TVCEEATCPNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNPLDENEPRHLAESVAA
HHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MRLKYVVVTSVDRDDLRDGGAQHFADCINAVREMSPATQIETLVPDFRGRLDIAVDILTQ
HHHHEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEHHC
TPPDVMNHNLETVPRLYKQARPGADYAHSLQLLKDYKAKNPNVRTKSGLMVGLGETDEEI
CCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
LEVMRDLRAHNVDMLTIGQYLQPSDGHLPVLRYVHPDVFKMFEEKAYEMGFVHAAVGAMV
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RSSYHADVQAHEAGV
HHHHHCCCHHHCCCC
>Mature Secondary Structure
MKPDNQAGVKHKGAAKTARIPIKIVPLDEKLKKPEWIRAKLPNGQRFHEIKQILREQKLH
CCCCCCCCCCCCCCCCEECCCEEEECCHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
TVCEEATCPNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNPLDENEPRHLAESVAA
HHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MRLKYVVVTSVDRDDLRDGGAQHFADCINAVREMSPATQIETLVPDFRGRLDIAVDILTQ
HHHHEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEHHC
TPPDVMNHNLETVPRLYKQARPGADYAHSLQLLKDYKAKNPNVRTKSGLMVGLGETDEEI
CCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
LEVMRDLRAHNVDMLTIGQYLQPSDGHLPVLRYVHPDVFKMFEEKAYEMGFVHAAVGAMV
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RSSYHADVQAHEAGV
HHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782