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The map label for this gene is rbsB [H]

Identifier: 34498471

GI number: 34498471

Start: 3293263

End: 3294198

Strand: Reverse

Name: rbsB [H]

Synonym: CV_3016

Alternate gene names: 34498471

Gene position: 3294198-3293263 (Counterclockwise)

Preceding gene: 34498472

Following gene: 34498470

Centisome position: 69.34

GC content: 67.31

Gene sequence:

>936_bases
ATGAAACGCATTCTTACTCCGCTCGTGGCCGGCATCCTGGCCTTCGGCATCGCCGCCTGCTCCAAGCAGGGCCCGGGCGG
CGCGCCGGCCGGCGACGCCAGCGCCGCGGCGGCAGACGGCAAGATCGCCGTCGGCCTGGCGGTGTCCACGCTCAACAACC
CGTTCTTCGTCGAGCTGCGCGACGGCGCGGCGGCCGAAGCCAAGAAACAGGGCGTCAACCTGATCACCGTCGACGCCCAG
GACGATCCGGCCAAGCAGCAGGCCAGCGTGGAAGACCTGATCCAGAAGAAGGTCAGCGTGATCCTGATCAACCCGACCGA
CTCCTCCGCCGTGGCCAACGTGGTGAAGGAAGCGACGTCCAAGGGCATCAAGGTGGTGTCGCTGGACCGCAGCGTCAACG
GCGCGGAAGTCAGCGCCCACATCGCGTCCGACAACATCGCCGGCGGCGTGATGGCCGGCAAGTACCTGCTGGACAAGCTG
GGCGGCAAGGGCCGCATCGTCGAGCTGGAAGGCATCGCCGGTTCTTCCGCCGCCCGCGAGCGCGGCGAGGGCTTCCACCA
GGTGGTGGACAAGAAGGACGGCGTCAAGCTGCTGGCCAAGCAGCCGGCCGACTTCGACCGCGCCAAGGGCCTGTCGGTGA
TGGAAAACATCATCCAGGGCAATAAGGACATCCAGGGCGTGTTCGCGCACAACGACGAAATGGCGCTGGGCGCGGTGAAG
GCGATCCAGGCAGCCGGCCTGAAGAACGTGGTGGTGGTGGGCTTCGATGCCACGCCGGACGCCGTGGCCGCGGTCAAGGG
CGGCACGCTGTCCGCCACCGTGCAGCAGCAGCCGGCGCTGATCGGCCAGTACGGCGTGCAGACCGCCAAGAAGCTGGCCG
ACGGCCAGAAGGTGGACAAGTTCATCCCGGTGCCGCTGAACCTGGTCCATCAGTAA

Upstream 100 bases:

>100_bases
TCCTGCTGGCGGTACTGCTGGATCGCAGCAACAAGAAGTGACGATGGCGGCGCGCGCCGCTCAATGCAGTGCATCATTCC
ATTTCTCAGGGAGCATCAGC

Downstream 100 bases:

>100_bases
AGCCCGCAGACAGAACCCCGGCCGCCGCTTGGCGGCCGGGGTTCTACCCGCTGGAGACGCGGTGCGGCAACGGGGCCGGC
GCAAGGCGCGCGGTCCCGTT

Product: ribose ABC transporter substrate binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKRILTPLVAGILAFGIAACSKQGPGGAPAGDASAAAADGKIAVGLAVSTLNNPFFVELRDGAAAEAKKQGVNLITVDAQ
DDPAKQQASVEDLIQKKVSVILINPTDSSAVANVVKEATSKGIKVVSLDRSVNGAEVSAHIASDNIAGGVMAGKYLLDKL
GGKGRIVELEGIAGSSAARERGEGFHQVVDKKDGVKLLAKQPADFDRAKGLSVMENIIQGNKDIQGVFAHNDEMALGAVK
AIQAAGLKNVVVVGFDATPDAVAAVKGGTLSATVQQQPALIGQYGVQTAKKLADGQKVDKFIPVPLNLVHQ

Sequences:

>Translated_311_residues
MKRILTPLVAGILAFGIAACSKQGPGGAPAGDASAAAADGKIAVGLAVSTLNNPFFVELRDGAAAEAKKQGVNLITVDAQ
DDPAKQQASVEDLIQKKVSVILINPTDSSAVANVVKEATSKGIKVVSLDRSVNGAEVSAHIASDNIAGGVMAGKYLLDKL
GGKGRIVELEGIAGSSAARERGEGFHQVVDKKDGVKLLAKQPADFDRAKGLSVMENIIQGNKDIQGVFAHNDEMALGAVK
AIQAAGLKNVVVVGFDATPDAVAAVKGGTLSATVQQQPALIGQYGVQTAKKLADGQKVDKFIPVPLNLVHQ
>Mature_311_residues
MKRILTPLVAGILAFGIAACSKQGPGGAPAGDASAAAADGKIAVGLAVSTLNNPFFVELRDGAAAEAKKQGVNLITVDAQ
DDPAKQQASVEDLIQKKVSVILINPTDSSAVANVVKEATSKGIKVVSLDRSVNGAEVSAHIASDNIAGGVMAGKYLLDKL
GGKGRIVELEGIAGSSAARERGEGFHQVVDKKDGVKLLAKQPADFDRAKGLSVMENIIQGNKDIQGVFAHNDEMALGAVK
AIQAAGLKNVVVVGFDATPDAVAAVKGGTLSATVQQQPALIGQYGVQTAKKLADGQKVDKFIPVPLNLVHQ

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=275, Percent_Identity=53.8181818181818, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1790526, Length=268, Percent_Identity=35.8208955223881, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790674, Length=230, Percent_Identity=34.3478260869565, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1789990, Length=270, Percent_Identity=28.1481481481481, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788473, Length=285, Percent_Identity=28.7719298245614, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790194, Length=237, Percent_Identity=25.3164556962025, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786540, Length=226, Percent_Identity=28.7610619469027, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31824; Mature: 31824

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILTPLVAGILAFGIAACSKQGPGGAPAGDASAAAADGKIAVGLAVSTLNNPFFVELR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEC
DGAAAEAKKQGVNLITVDAQDDPAKQQASVEDLIQKKVSVILINPTDSSAVANVVKEATS
CCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
KGIKVVSLDRSVNGAEVSAHIASDNIAGGVMAGKYLLDKLGGKGRIVELEGIAGSSAARE
CCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
RGEGFHQVVDKKDGVKLLAKQPADFDRAKGLSVMENIIQGNKDIQGVFAHNDEMALGAVK
HCCHHHHHHCCCCCCEEEECCCCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
AIQAAGLKNVVVVGFDATPDAVAAVKGGTLSATVQQQPALIGQYGVQTAKKLADGQKVDK
HHHHCCCCEEEEEEECCCCCHHHEECCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCHHCC
FIPVPLNLVHQ
CCCCCHHHCCC
>Mature Secondary Structure
MKRILTPLVAGILAFGIAACSKQGPGGAPAGDASAAAADGKIAVGLAVSTLNNPFFVELR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEC
DGAAAEAKKQGVNLITVDAQDDPAKQQASVEDLIQKKVSVILINPTDSSAVANVVKEATS
CCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
KGIKVVSLDRSVNGAEVSAHIASDNIAGGVMAGKYLLDKLGGKGRIVELEGIAGSSAARE
CCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
RGEGFHQVVDKKDGVKLLAKQPADFDRAKGLSVMENIIQGNKDIQGVFAHNDEMALGAVK
HCCHHHHHHCCCCCCEEEECCCCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
AIQAAGLKNVVVVGFDATPDAVAAVKGGTLSATVQQQPALIGQYGVQTAKKLADGQKVDK
HHHHCCCCEEEEEEECCCCCHHHEECCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCHHCC
FIPVPLNLVHQ
CCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]