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The map label for this gene is scrR [H]

Identifier: 34498470

GI number: 34498470

Start: 3292105

End: 3293064

Strand: Reverse

Name: scrR [H]

Synonym: CV_3015

Alternate gene names: 34498470

Gene position: 3293064-3292105 (Counterclockwise)

Preceding gene: 34498471

Following gene: 34498466

Centisome position: 69.31

GC content: 69.48

Gene sequence:

>960_bases
GTGTCGCGCACCACCGCCAGCATGGTGTTGAACGGCCATGCCGAGCGTTACCGCATCTCGCCGGCCACCGTGGAAAAAGT
GGAGCGGGTGGCGCGCGAGCACCATTTCAAGCCTTCCCAGCAGGCCCGCAGCCTGCGCAGCCGGCGCAGCAGCAGCATAG
GACTGGTGGTGCCGGACCTGACCAACTCCACCCACGCCGCGCTGGCGCAGGCGCTGGAGAACCGCTGCCGCGAACGGGGC
TACCAGTTGCTGATGGTGACCAGCGACGAAGACCCGGAACGGGAGAGCGACGGCATCGGCCAACTGGCCGCGCGCCAGGT
GGACGGCATGATCGTGGTGCCGTGCAGCGGCGACGCCGCGCGCTATCAGCCCTGGACCGGCCGGCTGCCGTTCGTGTTCG
CCGACCGCCATATTCCCGGCAGCGGCATCCCGTCGGCGGTCACCGACTCCGGCGACAGCGTCGCCCGGCTGCTGTCCCCG
CTGTTGGCCGGCGGGGTGGACGAGCTGGCATACTTTGGCGGCCAGCCGGAACTGTCGGCCAGCCGCGAGCGCCTGGCTGG
CTACCGGCAGGCGCTGCAAGCCGCGGGTTTGGAAGAGGGCGCAGACTGGGTGGCCGAGCGGGATTTCCAGCGCGAATCCG
GCTACCGGATGATGGCCGACTGGCATGCCCGCCACGGGCGCTATCCGCGCGCGCTGTTCACCGCGTCCATCACCTTGCTG
GAGGGCGTGCTGTCTTTCATCCGCGAACGGCATCGGCTGCGCGAGTCGCCGCGATATCTGCTCACTTTCGACGATCATCC
GCTGCTCGACTGCCTGCCGCTGCCGGTGGACGCCATATGCCAGGACAGCCGCGCGCTGGCCGAGGCCAGCCTGGGCCAGG
TGCTGGCGCTACTGCAGGGCGAGGCGTTGGCCGAGCGCGATCTGCGCGTGCCGGCCAGCCTTAATTTGCGCCGTATCTAA

Upstream 100 bases:

>100_bases
TTTCGCCGTATTGCGATTATCATTTCGCTTTCATCGCCCGGTCCGCCTCATGAGCAGCTTCAAACGCCTGACCATAGACG
ACATCGCCGCCCTGGCCGGC

Downstream 100 bases:

>100_bases
GCGCCCTGAGCGGCCTGGCGCGCGGCGGGCTCCCGCGTTTCGCGCATCAGCAGCGAGCCCAGCAGGAAGGTGGACGACAG
CAGCGTGGCGAACGCCATCG

Product: sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSRTTASMVLNGHAERYRISPATVEKVERVAREHHFKPSQQARSLRSRRSSSIGLVVPDLTNSTHAALAQALENRCRERG
YQLLMVTSDEDPERESDGIGQLAARQVDGMIVVPCSGDAARYQPWTGRLPFVFADRHIPGSGIPSAVTDSGDSVARLLSP
LLAGGVDELAYFGGQPELSASRERLAGYRQALQAAGLEEGADWVAERDFQRESGYRMMADWHARHGRYPRALFTASITLL
EGVLSFIRERHRLRESPRYLLTFDDHPLLDCLPLPVDAICQDSRALAEASLGQVLALLQGEALAERDLRVPASLNLRRI

Sequences:

>Translated_319_residues
MSRTTASMVLNGHAERYRISPATVEKVERVAREHHFKPSQQARSLRSRRSSSIGLVVPDLTNSTHAALAQALENRCRERG
YQLLMVTSDEDPERESDGIGQLAARQVDGMIVVPCSGDAARYQPWTGRLPFVFADRHIPGSGIPSAVTDSGDSVARLLSP
LLAGGVDELAYFGGQPELSASRERLAGYRQALQAAGLEEGADWVAERDFQRESGYRMMADWHARHGRYPRALFTASITLL
EGVLSFIRERHRLRESPRYLLTFDDHPLLDCLPLPVDAICQDSRALAEASLGQVLALLQGEALAERDLRVPASLNLRRI
>Mature_318_residues
SRTTASMVLNGHAERYRISPATVEKVERVAREHHFKPSQQARSLRSRRSSSIGLVVPDLTNSTHAALAQALENRCRERGY
QLLMVTSDEDPERESDGIGQLAARQVDGMIVVPCSGDAARYQPWTGRLPFVFADRHIPGSGIPSAVTDSGDSVARLLSPL
LAGGVDELAYFGGQPELSASRERLAGYRQALQAAGLEEGADWVAERDFQRESGYRMMADWHARHGRYPRALFTASITLLE
GVLSFIRERHRLRESPRYLLTFDDHPLLDCLPLPVDAICQDSRALAEASLGQVLALLQGEALAERDLRVPASLNLRRI

Specific function: Repressor for the scr operon. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=300, Percent_Identity=38.6666666666667, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=25.625, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787948, Length=251, Percent_Identity=27.0916334661355, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790369, Length=290, Percent_Identity=23.448275862069, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787906, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786540, Length=326, Percent_Identity=25.7668711656442, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789068, Length=314, Percent_Identity=23.5668789808917, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787580, Length=225, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35291; Mature: 35160

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTTASMVLNGHAERYRISPATVEKVERVAREHHFKPSQQARSLRSRRSSSIGLVVPDL
CCCCHHHHHHCCCCHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCC
TNSTHAALAQALENRCRERGYQLLMVTSDEDPERESDGIGQLAARQVDGMIVVPCSGDAA
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCC
RYQPWTGRLPFVFADRHIPGSGIPSAVTDSGDSVARLLSPLLAGGVDELAYFGGQPELSA
CCCCCCCCCCEEEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHH
SRERLAGYRQALQAAGLEEGADWVAERDFQRESGYRMMADWHARHGRYPRALFTASITLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCHHHHHHHHHHH
EGVLSFIRERHRLRESPRYLLTFDDHPLLDCLPLPVDAICQDSRALAEASLGQVLALLQG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHC
EALAERDLRVPASLNLRRI
CHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRTTASMVLNGHAERYRISPATVEKVERVAREHHFKPSQQARSLRSRRSSSIGLVVPDL
CCCHHHHHHCCCCHHEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCC
TNSTHAALAQALENRCRERGYQLLMVTSDEDPERESDGIGQLAARQVDGMIVVPCSGDAA
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCC
RYQPWTGRLPFVFADRHIPGSGIPSAVTDSGDSVARLLSPLLAGGVDELAYFGGQPELSA
CCCCCCCCCCEEEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHH
SRERLAGYRQALQAAGLEEGADWVAERDFQRESGYRMMADWHARHGRYPRALFTASITLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCHHHHHHHHHHH
EGVLSFIRERHRLRESPRYLLTFDDHPLLDCLPLPVDAICQDSRALAEASLGQVLALLQG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHC
EALAERDLRVPASLNLRRI
CHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412665 [H]