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The map label for this gene is dctS [H]

Identifier: 34497779

GI number: 34497779

Start: 2515008

End: 2516261

Strand: Reverse

Name: dctS [H]

Synonym: CV_2324

Alternate gene names: 34497779

Gene position: 2516261-2515008 (Counterclockwise)

Preceding gene: 34497780

Following gene: 34497778

Centisome position: 52.96

GC content: 63.64

Gene sequence:

>1254_bases
CTGATCGCGCTGCTGCCCGCCTATCTGCTGTGGGTGGTGCTGTCCTTGCTGGAATACAACCGTCAGGCCCGGGAACAGAT
CCAGGACGACATCTTCCGCGAACGCGAATACGCCAGCGTGGCCCTGCGCGGCATCAGCGATGCGGTGGTCAGCATAGACA
CCGAACAGGTGATCCAATACCTGAATCCGGCGGCGGAAGCCTTATTGGGGCTGCCCGCCGCGCAACTGCTGCAGCGTCGG
CTGAAACAGGTGGCGCCGCTGCACTACGAATTTTCGCGCCAGCCCGTGCAAGACCCGATCGAACAAGCCATCGCCACCCA
ACAAGTGGTTCATCTGGCGCAAAATTGCTACCTGAAGCGGCACAATGGAGAGAAACTGCTGGTGGAAGGCGCCGTGGCGC
CGTTGCTCGGACGCAACGGCCAACTGATCGGCAGCGTGCTGACCTTCCGCGACACTGCGCCCATCCGCCGCCGCATGCTG
GCGGCGCTGGAGGCCAGCGAAAGCCGACTGCGGCAACACGAACTGGAGCTGGCGCGCGTCACCCGCATCAACACCATGGG
GGAGATGGCCTCCGGCATCGCCCACGAAATCAATCAGCCGCTGTCGGCCATCATGAGCTACTGCCAGGCCGGCCTCAGCC
TGCTGGAAGACGACGAGCTGGACATGGCGATGCTGCGCCGGGCGCTGACCTCGTCGGTCAGCCAGGCCGACCGCGCCGGC
CGCATCATCCACCGCCTGCGGGAGTTCGTCACCCAGAAGAACCAGCAACTGGCGCCGGTACAGTTGAATCAGGCTGTGCA
CAACGCATTGTCGCTGCTGGATTACGAATTGCATGATCACGACATCCATATCGAGCAAAACTTCGCACCCGAGTTGCCGT
TGGTATACGCTGACACTATCCAGTTGGAACAGGTGGTGCTGAACCTGGCGCGCAACGCGGTGGAAGCCATGGAGCCAACC
CGTCCCTGGGGACGCCTGAGCATTACCACGCGCCGCCAGGGCGAGCGCGTGCAGCTCATCATCAGCGACAACGGCTGCGG
CATCCCCCCGGACAAGCTGGACCGGATTTTCGACCCCTTCTTCAGCACCAAGCCCAACGGCATGGGGCTGGGCCTGGCCA
TCTGCCAGACGGCGGTCGAGGCCTTCGGCGGCAAGCTGATAGCCGGCAACAAGCCCGGCTCGGGAGCGGAGTTCATCATT
GACCTGCCATGCGCCCCGCGCGACATGGCCGTACCATCCGGAGTGGAAGCCTGA

Upstream 100 bases:

>100_bases
CCGCGCCAGCCTCAACCTACCCGCCCTGCCCTACGACCTGTTGCTCAGCCGCCAGCTACGGCTGGAGCGCACCACCGCCG
CGGAAGCGGCCCTGTGCCTG

Downstream 100 bases:

>100_bases
TGCAAAACACGACCCCTACCGTCTTCATCGTCGACGATGATCCGGCCGTACTGGACTCCCTGGCCATGCTGATCACGGCC
CAGGGCATGAAAACCGTCAC

Product: two-component sensor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MIALLPAYLLWVVLSLLEYNRQAREQIQDDIFREREYASVALRGISDAVVSIDTEQVIQYLNPAAEALLGLPAAQLLQRR
LKQVAPLHYEFSRQPVQDPIEQAIATQQVVHLAQNCYLKRHNGEKLLVEGAVAPLLGRNGQLIGSVLTFRDTAPIRRRML
AALEASESRLRQHELELARVTRINTMGEMASGIAHEINQPLSAIMSYCQAGLSLLEDDELDMAMLRRALTSSVSQADRAG
RIIHRLREFVTQKNQQLAPVQLNQAVHNALSLLDYELHDHDIHIEQNFAPELPLVYADTIQLEQVVLNLARNAVEAMEPT
RPWGRLSITTRRQGERVQLIISDNGCGIPPDKLDRIFDPFFSTKPNGMGLGLAICQTAVEAFGGKLIAGNKPGSGAEFII
DLPCAPRDMAVPSGVEA

Sequences:

>Translated_417_residues
MIALLPAYLLWVVLSLLEYNRQAREQIQDDIFREREYASVALRGISDAVVSIDTEQVIQYLNPAAEALLGLPAAQLLQRR
LKQVAPLHYEFSRQPVQDPIEQAIATQQVVHLAQNCYLKRHNGEKLLVEGAVAPLLGRNGQLIGSVLTFRDTAPIRRRML
AALEASESRLRQHELELARVTRINTMGEMASGIAHEINQPLSAIMSYCQAGLSLLEDDELDMAMLRRALTSSVSQADRAG
RIIHRLREFVTQKNQQLAPVQLNQAVHNALSLLDYELHDHDIHIEQNFAPELPLVYADTIQLEQVVLNLARNAVEAMEPT
RPWGRLSITTRRQGERVQLIISDNGCGIPPDKLDRIFDPFFSTKPNGMGLGLAICQTAVEAFGGKLIAGNKPGSGAEFII
DLPCAPRDMAVPSGVEA
>Mature_417_residues
MIALLPAYLLWVVLSLLEYNRQAREQIQDDIFREREYASVALRGISDAVVSIDTEQVIQYLNPAAEALLGLPAAQLLQRR
LKQVAPLHYEFSRQPVQDPIEQAIATQQVVHLAQNCYLKRHNGEKLLVEGAVAPLLGRNGQLIGSVLTFRDTAPIRRRML
AALEASESRLRQHELELARVTRINTMGEMASGIAHEINQPLSAIMSYCQAGLSLLEDDELDMAMLRRALTSSVSQADRAG
RIIHRLREFVTQKNQQLAPVQLNQAVHNALSLLDYELHDHDIHIEQNFAPELPLVYADTIQLEQVVLNLARNAVEAMEPT
RPWGRLSITTRRQGERVQLIISDNGCGIPPDKLDRIFDPFFSTKPNGMGLGLAICQTAVEAFGGKLIAGNKPGSGAEFII
DLPCAPRDMAVPSGVEA

Specific function: Member of the two-component regulatory system dctS/dctR involved in the transport of C4-dicarboxylates. DctS functions as a membrane-associated protein kinase that phosphorylates dctR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=376, Percent_Identity=29.5212765957447, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=32.618025751073, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790300, Length=383, Percent_Identity=26.3707571801567, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693157, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994928, Length=399, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=148, Percent_Identity=34.4594594594595, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081816, Length=234, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=27.208480565371, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790567, Length=97, Percent_Identity=38.1443298969072, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=25.609756097561, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=126, Percent_Identity=32.5396825396825, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=27.1929824561404, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46259; Mature: 46259

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALLPAYLLWVVLSLLEYNRQAREQIQDDIFREREYASVALRGISDAVVSIDTEQVIQY
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LNPAAEALLGLPAAQLLQRRLKQVAPLHYEFSRQPVQDPIEQAIATQQVVHLAQNCYLKR
HCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
HNGEKLLVEGAVAPLLGRNGQLIGSVLTFRDTAPIRRRMLAALEASESRLRQHELELARV
CCCCEEEECCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRINTMGEMASGIAHEINQPLSAIMSYCQAGLSLLEDDELDMAMLRRALTSSVSQADRAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RIIHRLREFVTQKNQQLAPVQLNQAVHNALSLLDYELHDHDIHIEQNFAPELPLVYADTI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHH
QLEQVVLNLARNAVEAMEPTRPWGRLSITTRRQGERVQLIISDNGCGIPPDKLDRIFDPF
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHH
FSTKPNGMGLGLAICQTAVEAFGGKLIAGNKPGSGAEFIIDLPCAPRDMAVPSGVEA
HCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIALLPAYLLWVVLSLLEYNRQAREQIQDDIFREREYASVALRGISDAVVSIDTEQVIQY
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LNPAAEALLGLPAAQLLQRRLKQVAPLHYEFSRQPVQDPIEQAIATQQVVHLAQNCYLKR
HCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
HNGEKLLVEGAVAPLLGRNGQLIGSVLTFRDTAPIRRRMLAALEASESRLRQHELELARV
CCCCEEEECCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRINTMGEMASGIAHEINQPLSAIMSYCQAGLSLLEDDELDMAMLRRALTSSVSQADRAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RIIHRLREFVTQKNQQLAPVQLNQAVHNALSLLDYELHDHDIHIEQNFAPELPLVYADTI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHH
QLEQVVLNLARNAVEAMEPTRPWGRLSITTRRQGERVQLIISDNGCGIPPDKLDRIFDPF
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHH
FSTKPNGMGLGLAICQTAVEAFGGKLIAGNKPGSGAEFIIDLPCAPRDMAVPSGVEA
HCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8455557 [H]