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The map label for this gene is fixJ [H]

Identifier: 34497778

GI number: 34497778

Start: 2514334

End: 2515008

Strand: Reverse

Name: fixJ [H]

Synonym: CV_2323

Alternate gene names: 34497778

Gene position: 2515008-2514334 (Counterclockwise)

Preceding gene: 34497779

Following gene: 34497777

Centisome position: 52.94

GC content: 61.33

Gene sequence:

>675_bases
ATGCAAAACACGACCCCTACCGTCTTCATCGTCGACGATGATCCGGCCGTACTGGACTCCCTGGCCATGCTGATCACGGC
CCAGGGCATGAAAACCGTCACCTTCCCCAATGCGATGGAGTTCCTGGACGGCTACCAGGGCAACCAGATCTGCTGCCTGG
TGCTGGACATCCGCATGCCGCAGATCACCGGCCTCGCGCTGCAGGAACAACTGATGGAGCGGAACATCAGCATCCCCATC
GTCTTCATCACCGGCCATGGCGACATCGAGCAATGCCGCCGCGCGTTCCAGACCGGCGCCATCGATTTCCTGACCAAGCC
GATAGACCAGAACCGGCTGATCGCCAGCCTGCGCCGCGGCATACGCATCAGTATCCAGCAGCACCAGCAGGAAGAGGAAA
CCCAGGAGGTGATGAGCCAGCTCTCGCGCATCAGCGGCCGCGAACGCGAGGTGCTGGAGCTGGTGGCCGACGGCCTGTCC
AGCAAGGAGATCGCCCGCGAACTGGACCTGTCGCCTCGCACCATCGAAGTGCACCGCGCCAACCTGTTCAGCAAGCTGGG
CGTGGACTCGCTGGCCGATCTGGTCCGTTTCTACCTGAAAGCGCTGGAAGCCACCGGCAAGAAACAGCGGATAGACGAAG
AGATTTTGCTCAACGAATTCCCGCCAAACCAGTAG

Upstream 100 bases:

>100_bases
AGCTGATAGCCGGCAACAAGCCCGGCTCGGGAGCGGAGTTCATCATTGACCTGCCATGCGCCCCGCGCGACATGGCCGTA
CCATCCGGAGTGGAAGCCTG

Downstream 100 bases:

>100_bases
AATCGCCGCATGGAAAGATTGTTCAAACACATCTGCCTGGTGGCGCGCCACAGCAAGCCCGGCATCACCCCCGCGCTGAT
GCAGCTGGCCAACCACCTGG

Product: LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQNTTPTVFIVDDDPAVLDSLAMLITAQGMKTVTFPNAMEFLDGYQGNQICCLVLDIRMPQITGLALQEQLMERNISIPI
VFITGHGDIEQCRRAFQTGAIDFLTKPIDQNRLIASLRRGIRISIQQHQQEEETQEVMSQLSRISGREREVLELVADGLS
SKEIARELDLSPRTIEVHRANLFSKLGVDSLADLVRFYLKALEATGKKQRIDEEILLNEFPPNQ

Sequences:

>Translated_224_residues
MQNTTPTVFIVDDDPAVLDSLAMLITAQGMKTVTFPNAMEFLDGYQGNQICCLVLDIRMPQITGLALQEQLMERNISIPI
VFITGHGDIEQCRRAFQTGAIDFLTKPIDQNRLIASLRRGIRISIQQHQQEEETQEVMSQLSRISGREREVLELVADGLS
SKEIARELDLSPRTIEVHRANLFSKLGVDSLADLVRFYLKALEATGKKQRIDEEILLNEFPPNQ
>Mature_224_residues
MQNTTPTVFIVDDDPAVLDSLAMLITAQGMKTVTFPNAMEFLDGYQGNQICCLVLDIRMPQITGLALQEQLMERNISIPI
VFITGHGDIEQCRRAFQTGAIDFLTKPIDQNRLIASLRRGIRISIQQHQQEEETQEVMSQLSRISGREREVLELVADGLS
SKEIARELDLSPRTIEVHRANLFSKLGVDSLADLVRFYLKALEATGKKQRIDEEILLNEFPPNQ

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=34.375, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790102, Length=202, Percent_Identity=33.1683168316832, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=24.2574257425743, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788222, Length=203, Percent_Identity=23.6453201970443, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=29.0598290598291, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=158, Percent_Identity=25.9493670886076, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25280; Mature: 25280

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNTTPTVFIVDDDPAVLDSLAMLITAQGMKTVTFPNAMEFLDGYQGNQICCLVLDIRMP
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCEEEEEEEECCCC
QITGLALQEQLMERNISIPIVFITGHGDIEQCRRAFQTGAIDFLTKPIDQNRLIASLRRG
CHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCC
IRISIQQHQQEEETQEVMSQLSRISGREREVLELVADGLSSKEIARELDLSPRTIEVHRA
CEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHH
NLFSKLGVDSLADLVRFYLKALEATGKKQRIDEEILLNEFPPNQ
HHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MQNTTPTVFIVDDDPAVLDSLAMLITAQGMKTVTFPNAMEFLDGYQGNQICCLVLDIRMP
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCEEEEEEEECCCC
QITGLALQEQLMERNISIPIVFITGHGDIEQCRRAFQTGAIDFLTKPIDQNRLIASLRRG
CHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCC
IRISIQQHQQEEETQEVMSQLSRISGREREVLELVADGLSSKEIARELDLSPRTIEVHRA
CEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHH
NLFSKLGVDSLADLVRFYLKALEATGKKQRIDEEILLNEFPPNQ
HHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]