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The map label for this gene is ppnK

Identifier: 34497777

GI number: 34497777

Start: 2513449

End: 2514324

Strand: Reverse

Name: ppnK

Synonym: CV_2322

Alternate gene names: 34497777

Gene position: 2514324-2513449 (Counterclockwise)

Preceding gene: 34497778

Following gene: 34497776

Centisome position: 52.92

GC content: 66.1

Gene sequence:

>876_bases
ATGGAAAGATTGTTCAAACACATCTGCCTGGTGGCGCGCCACAGCAAGCCCGGCATCACCCCCGCGCTGATGCAGCTGGC
CAACCACCTGGCCGCCGGCGGCGCCACGGTGCTGATCGACAAGGAAAGCGTCACACCCGACGAGGCCAACGGTTACCCGC
TGATAGACCGCACCGACATGGGCAAGCTGGCCGACCTCTGCATCGTGCTGGGCGGCGACGGCACCATGCTGTCCATCGCC
CGCCTGCTGGCCCCGTACCGCGTGCCGCTGGTCGGCATCAACCAGGGCCGGCTGGGCTTCATGACCGACATCCCGCTGCA
CGAGATGCTGGATTCCGTGGACGCCATCCTGCACGGGAAATTCGTGCCGGAAGACCGCATCCTGCTGCAGGCGGCCGTTG
TCCGCGAGGATGCCGAAGTCGCCAGCGCGCTGGCCTTCAACGACGTGGTGTTCAGCCGCGGCGCGGTGGGCTCGATGATC
GAGTTCGAAGTCTTCATAGACAACCAGTTCGTCTACAGCCAGCGCTCGGATGGCCTGATCGTGTCCACGCCCACCGGCTC
CACCGCCTACTCGCTGGCGTCGGGCGGCCCCATCCTGCACCCGACGCTGCAGGCCATCGCGCTGGTGCCGATCTGCCCGC
AGTCGCTGTCCAACCGGCCGATTGCCGTCAACGACTCCTGCGAGGTGGAATTCATGCTCACCCGCGGGCTGGACGCGCGC
GTGCACTTCGACGGCCAGCTGCACTGCGACCTGATGGAAATGGACCGGGTGCTGATCCGCCGCTACCGCAACCCGCTGCG
CATCCTGCACCCCGAGGGCTACAACTACTACGACATGCTGCGCCACAAGCTGCACTGGGGCGAGCGCCTGATCTAA

Upstream 100 bases:

>100_bases
CCGTTTCTACCTGAAAGCGCTGGAAGCCACCGGCAAGAAACAGCGGATAGACGAAGAGATTTTGCTCAACGAATTCCCGC
CAAACCAGTAGAATCGCCGC

Downstream 100 bases:

>100_bases
CCGGAACAGACCACACACCATGCTTCTCTCGCTGACCGTCAAAGATTTCGTCATCGTCGACAGCATCGCGCTGGACTTTT
CCGGCGGCTTCACCGTGCTC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MERLFKHICLVARHSKPGITPALMQLANHLAAGGATVLIDKESVTPDEANGYPLIDRTDMGKLADLCIVLGGDGTMLSIA
RLLAPYRVPLVGINQGRLGFMTDIPLHEMLDSVDAILHGKFVPEDRILLQAAVVREDAEVASALAFNDVVFSRGAVGSMI
EFEVFIDNQFVYSQRSDGLIVSTPTGSTAYSLASGGPILHPTLQAIALVPICPQSLSNRPIAVNDSCEVEFMLTRGLDAR
VHFDGQLHCDLMEMDRVLIRRYRNPLRILHPEGYNYYDMLRHKLHWGERLI

Sequences:

>Translated_291_residues
MERLFKHICLVARHSKPGITPALMQLANHLAAGGATVLIDKESVTPDEANGYPLIDRTDMGKLADLCIVLGGDGTMLSIA
RLLAPYRVPLVGINQGRLGFMTDIPLHEMLDSVDAILHGKFVPEDRILLQAAVVREDAEVASALAFNDVVFSRGAVGSMI
EFEVFIDNQFVYSQRSDGLIVSTPTGSTAYSLASGGPILHPTLQAIALVPICPQSLSNRPIAVNDSCEVEFMLTRGLDAR
VHFDGQLHCDLMEMDRVLIRRYRNPLRILHPEGYNYYDMLRHKLHWGERLI
>Mature_291_residues
MERLFKHICLVARHSKPGITPALMQLANHLAAGGATVLIDKESVTPDEANGYPLIDRTDMGKLADLCIVLGGDGTMLSIA
RLLAPYRVPLVGINQGRLGFMTDIPLHEMLDSVDAILHGKFVPEDRILLQAAVVREDAEVASALAFNDVVFSRGAVGSMI
EFEVFIDNQFVYSQRSDGLIVSTPTGSTAYSLASGGPILHPTLQAIALVPICPQSLSNRPIAVNDSCEVEFMLTRGLDAR
VHFDGQLHCDLMEMDRVLIRRYRNPLRILHPEGYNYYDMLRHKLHWGERLI

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=326, Percent_Identity=27.6073619631902, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788968, Length=292, Percent_Identity=40.4109589041096, Blast_Score=222, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=31.6872427983539, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6322509, Length=244, Percent_Identity=29.5081967213115, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325068, Length=184, Percent_Identity=31.5217391304348, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573828, Length=257, Percent_Identity=32.295719844358, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28573826, Length=257, Percent_Identity=32.295719844358, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573830, Length=257, Percent_Identity=32.295719844358, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI161077047, Length=257, Percent_Identity=32.295719844358, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573832, Length=257, Percent_Identity=32.295719844358, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129957, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI281363321, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281363323, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653424, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653422, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_CHRVO (Q7NVM0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901992.1
- ProteinModelPortal:   Q7NVM0
- SMR:   Q7NVM0
- GeneID:   2549861
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2322
- NMPDR:   fig|243365.1.peg.2322
- HOGENOM:   HBG713904
- OMA:   FESFMID
- PhylomeDB:   Q7NVM0
- ProtClustDB:   PRK04539
- BioCyc:   CVIO243365:CV_2322-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32155; Mature: 32155

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLFKHICLVARHSKPGITPALMQLANHLAAGGATVLIDKESVTPDEANGYPLIDRTDM
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
GKLADLCIVLGGDGTMLSIARLLAPYRVPLVGINQGRLGFMTDIPLHEMLDSVDAILHGK
CCCEEEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHCCC
FVPEDRILLQAAVVREDAEVASALAFNDVVFSRGAVGSMIEFEVFIDNQFVYSQRSDGLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEECCCCCCEE
VSTPTGSTAYSLASGGPILHPTLQAIALVPICPQSLSNRPIAVNDSCEVEFMLTRGLDAR
EECCCCCCEEECCCCCCCCCHHHHHHEEECCCCHHHCCCCEEECCCCCEEEEEECCCCEE
VHFDGQLHCDLMEMDRVLIRRYRNPLRILHPEGYNYYDMLRHKLHWGERLI
EEECCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERLFKHICLVARHSKPGITPALMQLANHLAAGGATVLIDKESVTPDEANGYPLIDRTDM
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
GKLADLCIVLGGDGTMLSIARLLAPYRVPLVGINQGRLGFMTDIPLHEMLDSVDAILHGK
CCCEEEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHCCC
FVPEDRILLQAAVVREDAEVASALAFNDVVFSRGAVGSMIEFEVFIDNQFVYSQRSDGLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEECCCCCCEE
VSTPTGSTAYSLASGGPILHPTLQAIALVPICPQSLSNRPIAVNDSCEVEFMLTRGLDAR
EECCCCCCEEECCCCCCCCCHHHHHHEEECCCCHHHCCCCEEECCCCCEEEEEECCCCEE
VHFDGQLHCDLMEMDRVLIRRYRNPLRILHPEGYNYYDMLRHKLHWGERLI
EEECCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782