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The map label for this gene is prpC [H]

Identifier: 34497511

GI number: 34497511

Start: 2244175

End: 2245323

Strand: Reverse

Name: prpC [H]

Synonym: CV_2056

Alternate gene names: 34497511

Gene position: 2245323-2244175 (Counterclockwise)

Preceding gene: 34497512

Following gene: 34497510

Centisome position: 47.26

GC content: 64.23

Gene sequence:

>1149_bases
ATGACCGAAGTGGTTCTGACCGGCAAACCGAAGAAATCCGTTGCCCTGTCCGGCGTCGCCGCCGGCAACACCGCGCTGTG
CACCGTCGGCCGCAGCGGCAACGACCTGCACTATCGCGGCTACGACATCCTGGACTTGGCAGGCCACTGCGAATTCGAGG
AAGTCGCCCACCTGCTGGTGCACGGCAAGCTGCCCAACAAGGCCGAGCTCGCCGGCTACAAGAAGAAGCTGAAATCGCTG
CGCGGCCTGCCGGCCTCGGTCAAAGCCGCGCTGGAAGCGCTGCCCGCCGCCGCCCATCCGATGGACGTGATGCGCAGCGG
CGTGTCGGCGCTGGGCTGCGCGCTGCCCGAGAAGGACGACCACAACCTGGCCGGCGCCCGCGACATCGCCGACCGCCTGA
TGGCCTCGATGGGTTCCATGCTGCTCTATTGGTATCACTACAGCCACAACGGCAAGCGCATCGAGGTGGAAACCGACGAC
GACAGCATAGGCGGGCACTTCCTGCATCTGCTGCACGGGGTCAAGCCTTCCTACGAGTGGGTTCGCGCGATGCACACCTC
GCTGAACCTGTACGCCGAGCACGAATTCAACGCTTCCACCTTCACCGCCCGCGTCATCGCCGGCACCGGCTCCGACATGC
ACAGCGCCATCACCGGCGCCATCGGCGCGCTGCGCGGCCCGAAGCACGGCGGCGCCAACGAGGTGGCGTTTGAAATCCAG
CAGCGCTATGAAAATCCGGACGAAGCCGAAGCCGACATCCGCGCCCGCGTGGAACGCAAGGAAGTGGTGATCGGCTTCGG
CCACCCCGTTTATACGATTTCCGATCCGCGCAACAAGGTCATCAAGGAAGTGGCTCGCGAGCTGTCCAGGAGCAACAGCG
ACATGAAGATGTTCGACATCGCCGAACGGCTGGAGTCGGTGATGTGGGACGTCAAGAAAATGTTCCCCAACCTGGACTGG
TTCTCCGCCGTCAGCTACCACGCGATGGGCGTGCCGACCGCGATGTTCACGCCGCTGTTCGTGATCTCGCGCACCAGCGG
CTGGGCCGCTCACGTGATCGAGCAACGCCAGGACGGCAAGATCATCCGTCCGTCGGCCAATTACATCGGACCGGAAGACC
AGGCCTTCGTGCCGCTGGAGAAACGCTGA

Upstream 100 bases:

>100_bases
ACCACGAATACGAAAAGAAACTGGACCAGCTGTTCGCCGCCGGCAAGCAATAACAATCAAGGCAGCGGCATCGCCCCAAG
TCTGACAAAGGAGAAACAGC

Downstream 100 bases:

>100_bases
GCCATGCAGCAAGCGACGCGGCCGGACAGCCGCGTCGGCAAGACTGGAGCAGTCAATGAAGCAAATGTTGTTCTGGCAGC
GCCTGGACTGCCCGGGACTG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTEVVLTGKPKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDILDLAGHCEFEEVAHLLVHGKLPNKAELAGYKKKLKSL
RGLPASVKAALEALPAAAHPMDVMRSGVSALGCALPEKDDHNLAGARDIADRLMASMGSMLLYWYHYSHNGKRIEVETDD
DSIGGHFLHLLHGVKPSYEWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMHSAITGAIGALRGPKHGGANEVAFEIQ
QRYENPDEAEADIRARVERKEVVIGFGHPVYTISDPRNKVIKEVARELSRSNSDMKMFDIAERLESVMWDVKKMFPNLDW
FSAVSYHAMGVPTAMFTPLFVISRTSGWAAHVIEQRQDGKIIRPSANYIGPEDQAFVPLEKR

Sequences:

>Translated_382_residues
MTEVVLTGKPKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDILDLAGHCEFEEVAHLLVHGKLPNKAELAGYKKKLKSL
RGLPASVKAALEALPAAAHPMDVMRSGVSALGCALPEKDDHNLAGARDIADRLMASMGSMLLYWYHYSHNGKRIEVETDD
DSIGGHFLHLLHGVKPSYEWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMHSAITGAIGALRGPKHGGANEVAFEIQ
QRYENPDEAEADIRARVERKEVVIGFGHPVYTISDPRNKVIKEVARELSRSNSDMKMFDIAERLESVMWDVKKMFPNLDW
FSAVSYHAMGVPTAMFTPLFVISRTSGWAAHVIEQRQDGKIIRPSANYIGPEDQAFVPLEKR
>Mature_381_residues
TEVVLTGKPKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDILDLAGHCEFEEVAHLLVHGKLPNKAELAGYKKKLKSLR
GLPASVKAALEALPAAAHPMDVMRSGVSALGCALPEKDDHNLAGARDIADRLMASMGSMLLYWYHYSHNGKRIEVETDDD
SIGGHFLHLLHGVKPSYEWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMHSAITGAIGALRGPKHGGANEVAFEIQQ
RYENPDEAEADIRARVERKEVVIGFGHPVYTISDPRNKVIKEVARELSRSNSDMKMFDIAERLESVMWDVKKMFPNLDWF
SAVSYHAMGVPTAMFTPLFVISRTSGWAAHVIEQRQDGKIIRPSANYIGPEDQAFVPLEKR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=79.6791443850267, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1786939, Length=370, Percent_Identity=32.7027027027027, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17555174, Length=337, Percent_Identity=24.0356083086053, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319850, Length=345, Percent_Identity=24.0579710144928, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640126, Length=336, Percent_Identity=23.5119047619048, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640124, Length=336, Percent_Identity=23.5119047619048, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42031; Mature: 41900

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVVLTGKPKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDILDLAGHCEFEEVAHLLV
CCEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCEECCCEEEECCCCCCHHHHHHHHH
HGKLPNKAELAGYKKKLKSLRGLPASVKAALEALPAAAHPMDVMRSGVSALGCALPEKDD
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
HNLAGARDIADRLMASMGSMLLYWYHYSHNGKRIEVETDDDSIGGHFLHLLHGVKPSYEW
CCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCHHHH
VRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMHSAITGAIGALRGPKHGGANEVAFEIQ
HHHHHHHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QRYENPDEAEADIRARVERKEVVIGFGHPVYTISDPRNKVIKEVARELSRSNSDMKMFDI
HHCCCCCHHHHHHHHHHCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHH
AERLESVMWDVKKMFPNLDWFSAVSYHAMGVPTAMFTPLFVISRTSGWAAHVIEQRQDGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHEEECCCCHHHHHHHHCCCCC
IIRPSANYIGPEDQAFVPLEKR
EECCCCCCCCCCCCEECCCCCC
>Mature Secondary Structure 
TEVVLTGKPKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDILDLAGHCEFEEVAHLLV
CEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCEECCCEEEECCCCCCHHHHHHHHH
HGKLPNKAELAGYKKKLKSLRGLPASVKAALEALPAAAHPMDVMRSGVSALGCALPEKDD
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
HNLAGARDIADRLMASMGSMLLYWYHYSHNGKRIEVETDDDSIGGHFLHLLHGVKPSYEW
CCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCHHHH
VRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMHSAITGAIGALRGPKHGGANEVAFEIQ
HHHHHHHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QRYENPDEAEADIRARVERKEVVIGFGHPVYTISDPRNKVIKEVARELSRSNSDMKMFDI
HHCCCCCHHHHHHHHHHCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHH
AERLESVMWDVKKMFPNLDWFSAVSYHAMGVPTAMFTPLFVISRTSGWAAHVIEQRQDGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHEEECCCCHHHHHHHHCCCCC
IIRPSANYIGPEDQAFVPLEKR
EECCCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]