Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 34497512

GI number: 34497512

Start: 2245371

End: 2246249

Strand: Reverse

Name: prpB [H]

Synonym: CV_2057

Alternate gene names: 34497512

Gene position: 2246249-2245371 (Counterclockwise)

Preceding gene: 34497533

Following gene: 34497511

Centisome position: 47.28

GC content: 66.55

Gene sequence:

>879_bases
ATGAGCACTCCCGGGCAGCGTTTCCGCCAGGCCGTGGCCGCCGAAAAACCATTGCAAGTGGTGGGGGCGATCAACGCCTA
CGCTGCACGTTTGGCCGAACACTCAGGTTTCAAGGCCCTGTACCTGTCCGGCGGCGGCGTCGCCGCCTCGTCCTGCGGCA
TCCCGGACCTCGGCATCACCACGCTGGAAGACGTGCTGATCGACGCGCGCCGCATCACCGACAACACCGACCTGCCGCTG
CTGGTGGACATCGACACCGGCTGGGGCGGCGCCTTCAACATCGCCCGCGCGGTGCGCAGCCTGGAGAAGGCCGGCGTCGC
CGCCGTCCACATCGAAGACCAGGTGCAGCAGAAACGCTGCGGCCACCGCCCGAACAAGGCCATCGTACCTCAAGATGAGA
TGGTGGACCGCATCAAGGCCGCCACCGATGCCCGCCGCGACGCCAACTTCGTCATCATGGCGCGCACCGACGCGTTGGCC
GTGGAAGGCCTGGACGCCGCCATCGCGCGCGCCATCGCCTGTGTGGAGGCCGGCGCCGACATGATCTTCCCCGAAGCGAT
GACCGACCTGGCCATGTACAAGCGCTTCGCCGAAGCGGTGAAGGTGCCGGTGCTGGCCAACATCACCGAATTCGGCGCCA
CGCCGCTCTACACCACGGAAGAGCTGGCCGAGAACGGCGTCAGCCTGGTGCTGTACCCGCTGTCCGCCTTCCGCGCGATG
AGCCAGGCCGCGCTGGAAGTGTACGGCGCGATCCGCCGCGACGGCACCCAACAGGCGGTGCTGGACAAGATGCAGACCCG
GATGGAGCTGTACGACCACCTGGGCTACCACGAATACGAAAAGAAACTGGACCAGCTGTTCGCCGCCGGCAAGCAATAA

Upstream 100 bases:

>100_bases
AATATCAACTATTGCCAGCAATTTCTAGAGCCTTTACACTCCAACCAAGCACTTGCTTGAATGACAGGCAATTAATCCAA
TCCACCGTCAGGAGCATTCC

Downstream 100 bases:

>100_bases
CAATCAAGGCAGCGGCATCGCCCCAAGTCTGACAAAGGAGAAACAGCATGACCGAAGTGGTTCTGACCGGCAAACCGAAG
AAATCCGTTGCCCTGTCCGG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSTPGQRFRQAVAAEKPLQVVGAINAYAARLAEHSGFKALYLSGGGVAASSCGIPDLGITTLEDVLIDARRITDNTDLPL
LVDIDTGWGGAFNIARAVRSLEKAGVAAVHIEDQVQQKRCGHRPNKAIVPQDEMVDRIKAATDARRDANFVIMARTDALA
VEGLDAAIARAIACVEAGADMIFPEAMTDLAMYKRFAEAVKVPVLANITEFGATPLYTTEELAENGVSLVLYPLSAFRAM
SQAALEVYGAIRRDGTQQAVLDKMQTRMELYDHLGYHEYEKKLDQLFAAGKQ

Sequences:

>Translated_292_residues
MSTPGQRFRQAVAAEKPLQVVGAINAYAARLAEHSGFKALYLSGGGVAASSCGIPDLGITTLEDVLIDARRITDNTDLPL
LVDIDTGWGGAFNIARAVRSLEKAGVAAVHIEDQVQQKRCGHRPNKAIVPQDEMVDRIKAATDARRDANFVIMARTDALA
VEGLDAAIARAIACVEAGADMIFPEAMTDLAMYKRFAEAVKVPVLANITEFGATPLYTTEELAENGVSLVLYPLSAFRAM
SQAALEVYGAIRRDGTQQAVLDKMQTRMELYDHLGYHEYEKKLDQLFAAGKQ
>Mature_291_residues
STPGQRFRQAVAAEKPLQVVGAINAYAARLAEHSGFKALYLSGGGVAASSCGIPDLGITTLEDVLIDARRITDNTDLPLL
VDIDTGWGGAFNIARAVRSLEKAGVAAVHIEDQVQQKRCGHRPNKAIVPQDEMVDRIKAATDARRDANFVIMARTDALAV
EGLDAAIARAIACVEAGADMIFPEAMTDLAMYKRFAEAVKVPVLANITEFGATPLYTTEELAENGVSLVLYPLSAFRAMS
QAALEVYGAIRRDGTQQAVLDKMQTRMELYDHLGYHEYEKKLDQLFAAGKQ

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=289, Percent_Identity=66.0899653979239, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790445, Length=262, Percent_Identity=25.1908396946565, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31584; Mature: 31453

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPGQRFRQAVAAEKPLQVVGAINAYAARLAEHSGFKALYLSGGGVAASSCGIPDLGIT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCCCCCHH
TLEDVLIDARRITDNTDLPLLVDIDTGWGGAFNIARAVRSLEKAGVAAVHIEDQVQQKRC
HHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHC
GHRPNKAIVPQDEMVDRIKAATDARRDANFVIMARTDALAVEGLDAAIARAIACVEAGAD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHCCC
MIFPEAMTDLAMYKRFAEAVKVPVLANITEFGATPLYTTEELAENGVSLVLYPLSAFRAM
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCEEEEEHHHHHHHH
SQAALEVYGAIRRDGTQQAVLDKMQTRMELYDHLGYHEYEKKLDQLFAAGKQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STPGQRFRQAVAAEKPLQVVGAINAYAARLAEHSGFKALYLSGGGVAASSCGIPDLGIT
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCCCCCHH
TLEDVLIDARRITDNTDLPLLVDIDTGWGGAFNIARAVRSLEKAGVAAVHIEDQVQQKRC
HHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHC
GHRPNKAIVPQDEMVDRIKAATDARRDANFVIMARTDALAVEGLDAAIARAIACVEAGAD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHCCC
MIFPEAMTDLAMYKRFAEAVKVPVLANITEFGATPLYTTEELAENGVSLVLYPLSAFRAM
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCEEEEEHHHHHHHH
SQAALEVYGAIRRDGTQQAVLDKMQTRMELYDHLGYHEYEKKLDQLFAAGKQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]