Click here to switch to the map view.

The map label for this gene is potI [H]

Identifier: 34497414

GI number: 34497414

Start: 2131194

End: 2132033

Strand: Direct

Name: potI [H]

Synonym: CV_1959

Alternate gene names: 34497414

Gene position: 2131194-2132033 (Clockwise)

Preceding gene: 34497413

Following gene: 34497415

Centisome position: 44.86

GC content: 63.57

Gene sequence:

>840_bases
ATGATCAAGCCGAGCAAACCCTTGTCTTTCCTGGTGCTGGGGCTGGGCTACGCCTTCCTCTACCTGCCCATCGCGCTTCT
GGTGATCTATTCGTTCAACGAGTCCAAGCTGGTGACGGTGTGGTCCGGCTTCTCCACCAAGTGGTACGCGGCGCTGCTGG
AGGACGACGAGCTGATCAGCGCGGCCTGGCTGAGCCTGAAGATCGCGCTGATGACGGCGACGGCGTCGGTGGTGATGGGC
ACCTGGGTCGGCTTCGTGCTGGGGCGCTTTGGCCGCTTCCGCCTGTTCACGCTGTTTACCGGCATGGTCAACGCGCCGCT
GGTGATTCCGGAAGTGATACAGGGCATCTCGCTGCTGCTCTTGTTCGTGGCGATGGAGCAGAGCTTCGGCTGGCCGGAGG
GGCGCGGCGTGTTCACCATCTGGATCGGCCACGTGATGCTGTGCGTGTCCTACGTCGCCATCGTGGTGCAGTCGCGGGTG
CGGGAGCTGAACCTGTCGCTGGAGGAGGCGGCGATGGACCTGGGCGCGCGGCCGCTGAAGGTGTTCTTCGTCATCACGCT
GCCCTTGATCTCGCAGGCGCTGGTGTCCGGCTGGCTGCTGTCGTTCACGCTGTCGCTGGACGATCTGGTGCTGACCGCCT
TCCTGTCCGGTCCCGGCTCCACCACGCTGCCCATGGTGATTTTCTCCCGCGTGCGGCTGGGCCTGAATCCGGAAATGAAC
GCGCTGGCCACCTTGTTCATCGTGCTGGTGTCGATGGGCGTGGTGGCGGCCAACATCTATATGAGCGCGATGACGCGCAA
GCGGGAAAAGGAGATGAGGCTGGCTTTCGCCAACGGTTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCCGCCACCGTCACCTGCTGCATGGTATTGCTGCTGCTGGTGCCGATGGCGCTGTTCCAGCACTACCAGGTC
AAGAGCATGGAGGAAGCGCA

Downstream 100 bases:

>100_bases
GATCGAATGCGGTGAAAACGACAACGGCAGTGCGGCACAAGGAGAAATGACGATGAAGAGGTTACTGATCAGCGCGGCGA
TCGCGGCCCTGCCGGGCATG

Product: putrescine transport system permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIKPSKPLSFLVLGLGYAFLYLPIALLVIYSFNESKLVTVWSGFSTKWYAALLEDDELISAAWLSLKIALMTATASVVMG
TWVGFVLGRFGRFRLFTLFTGMVNAPLVIPEVIQGISLLLLFVAMEQSFGWPEGRGVFTIWIGHVMLCVSYVAIVVQSRV
RELNLSLEEAAMDLGARPLKVFFVITLPLISQALVSGWLLSFTLSLDDLVLTAFLSGPGSTTLPMVIFSRVRLGLNPEMN
ALATLFIVLVSMGVVAANIYMSAMTRKREKEMRLAFANG

Sequences:

>Translated_279_residues
MIKPSKPLSFLVLGLGYAFLYLPIALLVIYSFNESKLVTVWSGFSTKWYAALLEDDELISAAWLSLKIALMTATASVVMG
TWVGFVLGRFGRFRLFTLFTGMVNAPLVIPEVIQGISLLLLFVAMEQSFGWPEGRGVFTIWIGHVMLCVSYVAIVVQSRV
RELNLSLEEAAMDLGARPLKVFFVITLPLISQALVSGWLLSFTLSLDDLVLTAFLSGPGSTTLPMVIFSRVRLGLNPEMN
ALATLFIVLVSMGVVAANIYMSAMTRKREKEMRLAFANG
>Mature_279_residues
MIKPSKPLSFLVLGLGYAFLYLPIALLVIYSFNESKLVTVWSGFSTKWYAALLEDDELISAAWLSLKIALMTATASVVMG
TWVGFVLGRFGRFRLFTLFTGMVNAPLVIPEVIQGISLLLLFVAMEQSFGWPEGRGVFTIWIGHVMLCVSYVAIVVQSRV
RELNLSLEEAAMDLGARPLKVFFVITLPLISQALVSGWLLSFTLSLDDLVLTAFLSGPGSTTLPMVIFSRVRLGLNPEMN
ALATLFIVLVSMGVVAANIYMSAMTRKREKEMRLAFANG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=272, Percent_Identity=57.7205882352941, Blast_Score=280, Evalue=6e-77,
Organism=Escherichia coli, GI1787368, Length=257, Percent_Identity=36.5758754863813, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1787714, Length=248, Percent_Identity=29.8387096774194, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30704; Mature: 30704

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]