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The map label for this gene is potH [H]

Identifier: 34497413

GI number: 34497413

Start: 2130289

End: 2131197

Strand: Direct

Name: potH [H]

Synonym: CV_1958

Alternate gene names: 34497413

Gene position: 2130289-2131197 (Clockwise)

Preceding gene: 34497412

Following gene: 34497414

Centisome position: 44.84

GC content: 59.3

Gene sequence:

>909_bases
ATGAAACTACCGGATCTGAAACGTTGTCTGCCGACGGGCAGGGGGGCCGTGATAGGCGTGCCTTTCCTGTTTCTGTTCGT
GTTCTTCCTGCTACCTTTCCTGCTGGTGGTGGGTATCAGCTTTTCCCAGCAGCAGCTGGGCATTCCGCCGTATACGCCGC
TCACCAAGTTCGAGAACGACGTGATCGGCATCGCGCTCAACATCGGCCACTACAAGTTCATGCTGAGCGATGACCTGTAC
TTCGCCACCTACATCAGCTCGGTGAAGATGGCCTTCGTCTCCACCGTGCTGTGCCTGCTGATCGGCTACCCGATGGCTTA
CTACATCGCCAGGGCCGAGGGCGCGGCGCGCAACACGCTGATGATGATGGTGATGCTGCCGTTCTGGACCTCCTACCTGA
TCCGCGTCTACGCCTGGATCGGCATCCTGAAGAACGACGGCTTTCTCAACCAGTTCCTGCTGTGGCTGGGCGTGATCGAC
AGTCCGCTGCACCTGTACCACACCAATTGGGGCGTCTACATCGGCATGGTGTTTTCTTATCTGCCGTTCATGATCATGCC
GTTGTACGCCCACCTGGTGAAGATGGACCTGACGCTGCTGGAGGCCGCCTACGACCTGGGCGCCAAACCGTGGCGCGCGT
TCTGGCAGGTGACCTTGCCCCTGTCGAAGAACGGCATCATCGCCGGCAGCCTGCTGGTGTTCATCCCGGCGGTGGGCGAG
TACGTGATTCCGGAGCTGTTGGGCGGCTCGGACACGCTGATGATAGGACGGGTGATGTGGGAGGAGTTCTTCAACAATAT
GGATTGGCCGATGGCCGCCACCGTCACCTGCTGCATGGTATTGCTGCTGCTGGTGCCGATGGCGCTGTTCCAGCACTACC
AGGTCAAGAGCATGGAGGAAGCGCAATGA

Upstream 100 bases:

>100_bases
GTGGAACATCGACCGCCCGTCCGGGCGGGCGACGGGTATCGGCGGGCGTGCCGCCGATGGGGAAGCAACGCTTTGACGCC
TCGCGCGTCGTGGTGCCGCC

Downstream 100 bases:

>100_bases
TCAAGCCGAGCAAACCCTTGTCTTTCCTGGTGCTGGGGCTGGGCTACGCCTTCCTCTACCTGCCCATCGCGCTTCTGGTG
ATCTATTCGTTCAACGAGTC

Product: putrescine transport protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKLPDLKRCLPTGRGAVIGVPFLFLFVFFLLPFLLVVGISFSQQQLGIPPYTPLTKFENDVIGIALNIGHYKFMLSDDLY
FATYISSVKMAFVSTVLCLLIGYPMAYYIARAEGAARNTLMMMVMLPFWTSYLIRVYAWIGILKNDGFLNQFLLWLGVID
SPLHLYHTNWGVYIGMVFSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPWRAFWQVTLPLSKNGIIAGSLLVFIPAVGE
YVIPELLGGSDTLMIGRVMWEEFFNNMDWPMAATVTCCMVLLLLVPMALFQHYQVKSMEEAQ

Sequences:

>Translated_302_residues
MKLPDLKRCLPTGRGAVIGVPFLFLFVFFLLPFLLVVGISFSQQQLGIPPYTPLTKFENDVIGIALNIGHYKFMLSDDLY
FATYISSVKMAFVSTVLCLLIGYPMAYYIARAEGAARNTLMMMVMLPFWTSYLIRVYAWIGILKNDGFLNQFLLWLGVID
SPLHLYHTNWGVYIGMVFSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPWRAFWQVTLPLSKNGIIAGSLLVFIPAVGE
YVIPELLGGSDTLMIGRVMWEEFFNNMDWPMAATVTCCMVLLLLVPMALFQHYQVKSMEEAQ
>Mature_302_residues
MKLPDLKRCLPTGRGAVIGVPFLFLFVFFLLPFLLVVGISFSQQQLGIPPYTPLTKFENDVIGIALNIGHYKFMLSDDLY
FATYISSVKMAFVSTVLCLLIGYPMAYYIARAEGAARNTLMMMVMLPFWTSYLIRVYAWIGILKNDGFLNQFLLWLGVID
SPLHLYHTNWGVYIGMVFSYLPFMIMPLYAHLVKMDLTLLEAAYDLGAKPWRAFWQVTLPLSKNGIIAGSLLVFIPAVGE
YVIPELLGGSDTLMIGRVMWEEFFNNMDWPMAATVTCCMVLLLLVPMALFQHYQVKSMEEAQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=288, Percent_Identity=55.2083333333333, Blast_Score=321, Evalue=4e-89,
Organism=Escherichia coli, GI1787369, Length=270, Percent_Identity=41.8518518518518, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787713, Length=222, Percent_Identity=31.981981981982, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788764, Length=220, Percent_Identity=22.7272727272727, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34274; Mature: 34274

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPDLKRCLPTGRGAVIGVPFLFLFVFFLLPFLLVVGISFSQQQLGIPPYTPLTKFEND
CCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHCCCC
VIGIALNIGHYKFMLSDDLYFATYISSVKMAFVSTVLCLLIGYPMAYYIARAEGAARNTL
EEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MMMVMLPFWTSYLIRVYAWIGILKNDGFLNQFLLWLGVIDSPLHLYHTNWGVYIGMVFSY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
LPFMIMPLYAHLVKMDLTLLEAAYDLGAKPWRAFWQVTLPLSKNGIIAGSLLVFIPAVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHH
YVIPELLGGSDTLMIGRVMWEEFFNNMDWPMAATVTCCMVLLLLVPMALFQHYQVKSMEE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
AQ
CC
>Mature Secondary Structure
MKLPDLKRCLPTGRGAVIGVPFLFLFVFFLLPFLLVVGISFSQQQLGIPPYTPLTKFEND
CCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHCCCC
VIGIALNIGHYKFMLSDDLYFATYISSVKMAFVSTVLCLLIGYPMAYYIARAEGAARNTL
EEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MMMVMLPFWTSYLIRVYAWIGILKNDGFLNQFLLWLGVIDSPLHLYHTNWGVYIGMVFSY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
LPFMIMPLYAHLVKMDLTLLEAAYDLGAKPWRAFWQVTLPLSKNGIIAGSLLVFIPAVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHH
YVIPELLGGSDTLMIGRVMWEEFFNNMDWPMAATVTCCMVLLLLVPMALFQHYQVKSMEE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]