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The map label for this gene is metY [H]

Identifier: 34497389

GI number: 34497389

Start: 2104191

End: 2105468

Strand: Reverse

Name: metY [H]

Synonym: CV_1934

Alternate gene names: 34497389

Gene position: 2105468-2104191 (Counterclockwise)

Preceding gene: 34497390

Following gene: 34497387

Centisome position: 44.32

GC content: 66.12

Gene sequence:

>1278_bases
ATGAAACTGGAAACCCTCGCCGTCCACGCCGGCTACAGCCCCGACCCGACCACCAAGGCGGTGGCGGTGCCGCTGTACCA
GACCACCAGCTACGCCTTCGACAGCACCCAGCATGGCGCCGACCTGTTCGATCTCAAGGTGCAGGGCAATATCTACACCC
GCATCATGAATCCCACCACCGACGTGCTGGAAAAGAGGATAGCGGCGCTGGAGGGCGGCGTCGGCGCGCTGGCGGTGGCC
TCAGGCATGGCGGCCGTCAGCTATTCGATCCAGACCATCGCCGAGGCCGGCGACAACATCGTCGCCACCCGCACGCTGTA
CGGCGGCACCTACAACCTGTTCGCCCACACCTTCCCGCAGCTGGGCATCGAAGTCCGCTTCGTCGACTACCAGGACCCGG
CAGCCATCGCCGCGCTGGTGGATGACCGCACCAAGGCGGTGTTCTGCGAATCGATAGGCAATCCGCTGGGCAATGTGGTG
GACTTCGCCGCCTTCGCCGCCGAAGCCCACCGCCACGGCCTGCCGCTGATCGTCGACAACACCGTGCCCTCGCCCTACCT
GTGCCGGCCGTTCGAGCACGGCGCCGACATCGTGGTCCACAGCCTGACCAAATACCTGGGCGGCCACGGCAACAGCATAG
GCGGCGCCATTGTCGACTCCGGCAAATTCCCATGGGGGCGACACAAGACGCGCTTCAAGCGGCTGAACGAGCCGGATGTC
AGCTACCATGGCGTCAATTACGTGGAAGCGCTGGGCGAGGCCGCCTACATCGCCCGCGCCCGCGTGGTGCCGCTGCGCAA
TATGGGCGCGGCGCTGTCGCCGTTCAACGCCTTCCTGATCCTGCAGGGCATAGAAACGCTGGCGCTGCGCATGGACCGCA
TTTGCGACAACGCATTGAAAGTAGCGGGCTTCCTGAAGGAAAGCGCGGCGGTGGAATGGGTGGAATACGCCGGCCTGCCT
GACCACCCCAGCAAGCCGCTGGCCGACCGCTATCTGGGCGGCCGCGCCTCCGGCATCCTGTCTTTCGGCATCAAGGGCGG
CGCCGAAGCCGGCGGCCGCTTCATCGACGCGCTGCGGCTGGTGACGCGGCTGGTCAATATCGGCGACGCCAAGTCGCTGG
CCTGCCACCCCGCCACCACTACCCACCGCCAGCTTTCGCCGGAGGAGCAACTGAGCGCCGGGGTAAAGCCGGAACTGATC
CGGCTGTCCATTGGCATCGAGCACATCGACGACATCCTGGCCGACATCGGCCAGGCGCTGAAAGCCGCGGCCCAGTAA

Upstream 100 bases:

>100_bases
CGGACAGTCATATCCAGTATGATTTTTATGTCCTTGTGATCGACAGCCGGCGGCGCTAAGGTAGACAGCCATGACAAACG
CCTGACCAGGAGCCGCCACG

Downstream 100 bases:

>100_bases
GCCCAGGCATGGGACGAACCGTCCCAAACGAAACGGGGCCGCGAAAGCGGCCCCCGGTCCATCGCATCAGGCCTGCGGCC
TGAGCGTGGCTGACAAAACT

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKLETLAVHAGYSPDPTTKAVAVPLYQTTSYAFDSTQHGADLFDLKVQGNIYTRIMNPTTDVLEKRIAALEGGVGALAVA
SGMAAVSYSIQTIAEAGDNIVATRTLYGGTYNLFAHTFPQLGIEVRFVDYQDPAAIAALVDDRTKAVFCESIGNPLGNVV
DFAAFAAEAHRHGLPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGRHKTRFKRLNEPDV
SYHGVNYVEALGEAAYIARARVVPLRNMGAALSPFNAFLILQGIETLALRMDRICDNALKVAGFLKESAAVEWVEYAGLP
DHPSKPLADRYLGGRASGILSFGIKGGAEAGGRFIDALRLVTRLVNIGDAKSLACHPATTTHRQLSPEEQLSAGVKPELI
RLSIGIEHIDDILADIGQALKAAAQ

Sequences:

>Translated_425_residues
MKLETLAVHAGYSPDPTTKAVAVPLYQTTSYAFDSTQHGADLFDLKVQGNIYTRIMNPTTDVLEKRIAALEGGVGALAVA
SGMAAVSYSIQTIAEAGDNIVATRTLYGGTYNLFAHTFPQLGIEVRFVDYQDPAAIAALVDDRTKAVFCESIGNPLGNVV
DFAAFAAEAHRHGLPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGRHKTRFKRLNEPDV
SYHGVNYVEALGEAAYIARARVVPLRNMGAALSPFNAFLILQGIETLALRMDRICDNALKVAGFLKESAAVEWVEYAGLP
DHPSKPLADRYLGGRASGILSFGIKGGAEAGGRFIDALRLVTRLVNIGDAKSLACHPATTTHRQLSPEEQLSAGVKPELI
RLSIGIEHIDDILADIGQALKAAAQ
>Mature_425_residues
MKLETLAVHAGYSPDPTTKAVAVPLYQTTSYAFDSTQHGADLFDLKVQGNIYTRIMNPTTDVLEKRIAALEGGVGALAVA
SGMAAVSYSIQTIAEAGDNIVATRTLYGGTYNLFAHTFPQLGIEVRFVDYQDPAAIAALVDDRTKAVFCESIGNPLGNVV
DFAAFAAEAHRHGLPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGRHKTRFKRLNEPDV
SYHGVNYVEALGEAAYIARARVVPLRNMGAALSPFNAFLILQGIETLALRMDRICDNALKVAGFLKESAAVEWVEYAGLP
DHPSKPLADRYLGGRASGILSFGIKGGAEAGGRFIDALRLVTRLVNIGDAKSLACHPATTTHRQLSPEEQLSAGVKPELI
RLSIGIEHIDDILADIGQALKAAAQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.9881796690307, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=31.6784869976359, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.8056872037915, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1790375, Length=422, Percent_Identity=31.9905213270142, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=27.7389277389277, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71980708, Length=435, Percent_Identity=30.8045977011494, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=34.4339622641509, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=35.1415094339623, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=48.8425925925926, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=433, Percent_Identity=33.0254041570439, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=26.5765765765766, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=358, Percent_Identity=29.3296089385475, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=23.3256351039261, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=33.7236533957845, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45428; Mature: 45428

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETLAVHAGYSPDPTTKAVAVPLYQTTSYAFDSTQHGADLFDLKVQGNIYTRIMNPTT
CCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCHH
DVLEKRIAALEGGVGALAVASGMAAVSYSIQTIAEAGDNIVATRTLYGGTYNLFAHTFPQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHC
LGIEVRFVDYQDPAAIAALVDDRTKAVFCESIGNPLGNVVDFAAFAAEAHRHGLPLIVDN
CCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEC
TVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGRHKTRFKRLNEPDV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCC
SYHGVNYVEALGEAAYIARARVVPLRNMGAALSPFNAFLILQGIETLALRMDRICDNALK
CCCCCHHHHHHHHHHHHHHHEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGFLKESAAVEWVEYAGLPDHPSKPLADRYLGGRASGILSFGIKGGAEAGGRFIDALRL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHH
VTRLVNIGDAKSLACHPATTTHRQLSPEEQLSAGVKPELIRLSIGIEHIDDILADIGQAL
HHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHH
KAAAQ
HHHCC
>Mature Secondary Structure
MKLETLAVHAGYSPDPTTKAVAVPLYQTTSYAFDSTQHGADLFDLKVQGNIYTRIMNPTT
CCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCHH
DVLEKRIAALEGGVGALAVASGMAAVSYSIQTIAEAGDNIVATRTLYGGTYNLFAHTFPQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHC
LGIEVRFVDYQDPAAIAALVDDRTKAVFCESIGNPLGNVVDFAAFAAEAHRHGLPLIVDN
CCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEC
TVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGRHKTRFKRLNEPDV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCC
SYHGVNYVEALGEAAYIARARVVPLRNMGAALSPFNAFLILQGIETLALRMDRICDNALK
CCCCCHHHHHHHHHHHHHHHEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGFLKESAAVEWVEYAGLPDHPSKPLADRYLGGRASGILSFGIKGGAEAGGRFIDALRL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHH
VTRLVNIGDAKSLACHPATTTHRQLSPEEQLSAGVKPELIRLSIGIEHIDDILADIGQAL
HHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHH
KAAAQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]