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The map label for this gene is rumA

Identifier: 34497387

GI number: 34497387

Start: 2101407

End: 2102951

Strand: Reverse

Name: rumA

Synonym: CV_1932

Alternate gene names: 34497387

Gene position: 2102951-2101407 (Counterclockwise)

Preceding gene: 34497389

Following gene: 34497386

Centisome position: 44.26

GC content: 64.47

Gene sequence:

>1545_bases
ATGACGCACCCACAGACTATTGCCCTGGTCGAATCGCTGGATCACGAAGGCCGGGGGGTCGCCCACGTTGACGGCAAAAC
CCTCTTCATCGATGGCGCGCTGCCGTATGAAAAGGTTATCTACAGCAGCTACCGCAAGAAGCCCAGCTACGAAAACGCGC
AGACCAGCGCCGTGCTGAAGGAAAGCTTCATGCGCGCCGATCCGCGCTGCCCGCACTTCGGCGTCTGCGGCGGCTGCTCG
ATGCAGCACGTGGAATTCACCGCCCAGGTGGCGATCAAGCAACGCGTGCTGGAAGACAATCTGAAGCGCATCGGCAAGGT
CAAGGCCGAGCGCGTGCTCACCCCCATCGCCGGACCGGCCTGGCACTACCGTCATCGCGCCCGCCTGTCCGCCCGCATGG
TGGCCAAAAAGGGCGGCGTGCTGGTCGGCTTCCATGAGAAGAGCTCCAGCTACATTGCCGAGATGCGCGAGTGCCACATC
CTGCCCAAGCACATCTCCGACCTGATCATGCCGCTGCGCGAAATGATCGCCACGCTGTCCATCAGCCAACGCATGCCGCA
GGTGGAAGTGGCGGTCGGCGACAAGCTCGACATCCTGGTGTTCCGCAATATGGACGACATCACCGATGCCGACTTCGCCA
TCCTGAAGGCGTTTTCCGACAAGCACGGCGCGGCCGAGCGGCCGCTGCAGATCTGGCTGCAGCCCAAGGGGCCGGACACC
TGCTACCCGATCTATCCGCTGGACGCGCCGAAGCTGACCTACAGCATGCCGGAATTCGCGGTGGAGATGCCCTACTACCC
GACCGAATTCACCCAGGTGAACCCGCAGATCAACGCGGTGATGGTGGCGCGCGCGCTGAAGTTCCTGGATCCGCAGCCGG
GCGAGCGCATCGCCGACATGTTCTGCGGCATCGGCAACTTCACCCTGCCCATCGCCCGTTCCGGCGCCGTGGTGCACGGC
ATGGAAGGCAGCCAGCCGCTGGTGAGGCGTGCGGTGGAAAACGCCACCCACAACGGTCTGCAGGACAAGGTGAGCTACGA
AATGGCCAACCTGTTCGACGTGACCGAGGAGTCGTTCGCGGCGCTTGGCAAGTTCGACAAGATGCTGGTCGATCCGCCGC
GCGACGGCGCGGTGCAGCTGTTGAAGGCCATCACCGAGGAGACCGCGCCGCAACGCATCGTCTACGTGTCCTGTAATCCG
TCCACGCTGGCGCGCGACGCCGGCGTGCTGGTGCACACCAAGGGCTACACGCTGAAGGCGGCCGGCATCATCAATATGTT
CCCGCACACCGCGCACGTAGAGTCGGTGGCCTGGTTCGAGAAGACCGGCCCCTGCAAGACGCGCAAGGAAGTCGAGGAAA
TCGAGGCGCTGGAAGCCGCCGAACGCGAGGCCGCCAAAGCGGCGCGCCTGGCGGAAGAAAACGCGGAGAAGGCAAGAAAG
GCCGCCGAGCTGGCGGAGAAAGCCGCCGCCAAGGAAGCCCGCCGCGCGCACTACTTCGCGGAGAAAGCCCGCCGCGACGC
CGAGGAAGCCGCTTCGGATCAATGA

Upstream 100 bases:

>100_bases
AACAGAAAGGCCAGGAATTCCGGGATCGTAAAAAAACCAGCAAGAATCCCCGGTTTTTGAGAAAATCCGCGTTTTACCGT
TTGATTTTGCAAGCAAGACG

Downstream 100 bases:

>100_bases
CGGGACAACGGCCCGTTTTGGCGACTTCGCCAAAGCGGGCCGTTTATCTTTACCTAAAGACAGATTGTTTGATCTAAAGA
CAAGTATTATCCTCCGCTTC

Product: 23S rRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTHPQTIALVESLDHEGRGVAHVDGKTLFIDGALPYEKVIYSSYRKKPSYENAQTSAVLKESFMRADPRCPHFGVCGGCS
MQHVEFTAQVAIKQRVLEDNLKRIGKVKAERVLTPIAGPAWHYRHRARLSARMVAKKGGVLVGFHEKSSSYIAEMRECHI
LPKHISDLIMPLREMIATLSISQRMPQVEVAVGDKLDILVFRNMDDITDADFAILKAFSDKHGAAERPLQIWLQPKGPDT
CYPIYPLDAPKLTYSMPEFAVEMPYYPTEFTQVNPQINAVMVARALKFLDPQPGERIADMFCGIGNFTLPIARSGAVVHG
MEGSQPLVRRAVENATHNGLQDKVSYEMANLFDVTEESFAALGKFDKMLVDPPRDGAVQLLKAITEETAPQRIVYVSCNP
STLARDAGVLVHTKGYTLKAAGIINMFPHTAHVESVAWFEKTGPCKTRKEVEEIEALEAAEREAAKAARLAEENAEKARK
AAELAEKAAAKEARRAHYFAEKARRDAEEAASDQ

Sequences:

>Translated_514_residues
MTHPQTIALVESLDHEGRGVAHVDGKTLFIDGALPYEKVIYSSYRKKPSYENAQTSAVLKESFMRADPRCPHFGVCGGCS
MQHVEFTAQVAIKQRVLEDNLKRIGKVKAERVLTPIAGPAWHYRHRARLSARMVAKKGGVLVGFHEKSSSYIAEMRECHI
LPKHISDLIMPLREMIATLSISQRMPQVEVAVGDKLDILVFRNMDDITDADFAILKAFSDKHGAAERPLQIWLQPKGPDT
CYPIYPLDAPKLTYSMPEFAVEMPYYPTEFTQVNPQINAVMVARALKFLDPQPGERIADMFCGIGNFTLPIARSGAVVHG
MEGSQPLVRRAVENATHNGLQDKVSYEMANLFDVTEESFAALGKFDKMLVDPPRDGAVQLLKAITEETAPQRIVYVSCNP
STLARDAGVLVHTKGYTLKAAGIINMFPHTAHVESVAWFEKTGPCKTRKEVEEIEALEAAEREAAKAARLAEENAEKARK
AAELAEKAAAKEARRAHYFAEKARRDAEEAASDQ
>Mature_513_residues
THPQTIALVESLDHEGRGVAHVDGKTLFIDGALPYEKVIYSSYRKKPSYENAQTSAVLKESFMRADPRCPHFGVCGGCSM
QHVEFTAQVAIKQRVLEDNLKRIGKVKAERVLTPIAGPAWHYRHRARLSARMVAKKGGVLVGFHEKSSSYIAEMRECHIL
PKHISDLIMPLREMIATLSISQRMPQVEVAVGDKLDILVFRNMDDITDADFAILKAFSDKHGAAERPLQIWLQPKGPDTC
YPIYPLDAPKLTYSMPEFAVEMPYYPTEFTQVNPQINAVMVARALKFLDPQPGERIADMFCGIGNFTLPIARSGAVVHGM
EGSQPLVRRAVENATHNGLQDKVSYEMANLFDVTEESFAALGKFDKMLVDPPRDGAVQLLKAITEETAPQRIVYVSCNPS
TLARDAGVLVHTKGYTLKAAGIINMFPHTAHVESVAWFEKTGPCKTRKEVEEIEALEAAEREAAKAARLAEENAEKARKA
AELAEKAAAKEARRAHYFAEKARRDAEEAASDQ

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumA subfamily

Homologues:

Organism=Escherichia coli, GI1789148, Length=438, Percent_Identity=38.8127853881278, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=23.4986945169713, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24666579, Length=225, Percent_Identity=29.3333333333333, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_CHRVO (Q7NWP9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901602.1
- ProteinModelPortal:   Q7NWP9
- SMR:   Q7NWP9
- GeneID:   2547247
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1932
- NMPDR:   fig|243365.1.peg.1932
- HOGENOM:   HBG690163
- OMA:   AVENATH
- PhylomeDB:   Q7NWP9
- ProtClustDB:   CLSK2485433
- BioCyc:   CVIO243365:CV_1932-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 57026; Mature: 56895

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 398-398 BINDING 272-272 BINDING 301-301 BINDING 306-306 BINDING 322-322 BINDING 350-350 BINDING 371-371

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPQTIALVESLDHEGRGVAHVDGKTLFIDGALPYEKVIYSSYRKKPSYENAQTSAVLK
CCCCHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
ESFMRADPRCPHFGVCGGCSMQHVEFTAQVAIKQRVLEDNLKRIGKVKAERVLTPIAGPA
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
WHYRHRARLSARMVAKKGGVLVGFHEKSSSYIAEMRECHILPKHISDLIMPLREMIATLS
HHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ISQRMPQVEVAVGDKLDILVFRNMDDITDADFAILKAFSDKHGAAERPLQIWLQPKGPDT
HHHCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
CYPIYPLDAPKLTYSMPEFAVEMPYYPTEFTQVNPQINAVMVARALKFLDPQPGERIADM
EEEECCCCCCCCEECCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
FCGIGNFTLPIARSGAVVHGMEGSQPLVRRAVENATHNGLQDKVSYEMANLFDVTEESFA
HHCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ALGKFDKMLVDPPRDGAVQLLKAITEETAPQRIVYVSCNPSTLARDAGVLVHTKGYTLKA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCEEEEECCCEEEE
AGIINMFPHTAHVESVAWFEKTGPCKTRKEVEEIEALEAAEREAAKAARLAEENAEKARK
CCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAELAEKAAAKEARRAHYFAEKARRDAEEAASDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
THPQTIALVESLDHEGRGVAHVDGKTLFIDGALPYEKVIYSSYRKKPSYENAQTSAVLK
CCCHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
ESFMRADPRCPHFGVCGGCSMQHVEFTAQVAIKQRVLEDNLKRIGKVKAERVLTPIAGPA
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
WHYRHRARLSARMVAKKGGVLVGFHEKSSSYIAEMRECHILPKHISDLIMPLREMIATLS
HHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ISQRMPQVEVAVGDKLDILVFRNMDDITDADFAILKAFSDKHGAAERPLQIWLQPKGPDT
HHHCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
CYPIYPLDAPKLTYSMPEFAVEMPYYPTEFTQVNPQINAVMVARALKFLDPQPGERIADM
EEEECCCCCCCCEECCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
FCGIGNFTLPIARSGAVVHGMEGSQPLVRRAVENATHNGLQDKVSYEMANLFDVTEESFA
HHCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ALGKFDKMLVDPPRDGAVQLLKAITEETAPQRIVYVSCNPSTLARDAGVLVHTKGYTLKA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCEEEEECCCEEEE
AGIINMFPHTAHVESVAWFEKTGPCKTRKEVEEIEALEAAEREAAKAARLAEENAEKARK
CCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAELAEKAAAKEARRAHYFAEKARRDAEEAASDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782