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The map label for this gene is pflB [H]

Identifier: 34496867

GI number: 34496867

Start: 1488143

End: 1490470

Strand: Direct

Name: pflB [H]

Synonym: CV_1412

Alternate gene names: 34496867

Gene position: 1488143-1490470 (Clockwise)

Preceding gene: 34496866

Following gene: 34496868

Centisome position: 31.32

GC content: 64.13

Gene sequence:

>2328_bases
ATGGACGCCATCACTCAGAATGCTACCCAAGCCAACCCGTGGCGCGATTTCGTCGCCGGCGACTGGCAGAGCAAGGTCGA
TGTCCGCAACTTCATCCAGCTGAACTATCAGCCGTACGAAGGCGACGACAGCTTCCTGGCTGGCGCCACCGAGCGCACCA
AGAAGCTGTGGGATACCCTGGGCGAACTGCTGAAGCAGGAACGCGCCAAGGGCGTGCTGGACGTGTCCGCCGACCGCGGC
TCCTCCATCACCGCGCATGACGCCGGCTACATAGACCAGGCCAACGAAGTGATCGTCGGCCTGCAAACCGACGCCCCGCT
GAAGCGCGCCATCATGCCGAACGGCGGCCTGCGCATGGTCGAGAACGGCCTGGAAGCCTTCGGCTTCAAGCTGGACCCGA
TGATCAAGGAAGTGTGGGAAAAGTATCGCAAGAGCCACAACCAGGGCGTGTTCGACGTCTACACCCCGGAAATCATGGCT
TGCCGCAAGTCCGGCGTGATCACCGGCCTGCCCGACGCCTACGGCCGCGGCCGCATCATCGGCGACTACCGCCGCGTGGC
CCTGTACGGCACCGACTTCCTGCGCGCCGAGCGCCAGCAAGTGTTCCATGAACTGGACAACGCTACCTTCACCGACGACG
TGATGCGTCTGCGCGAAGAGCTGTCCGAACAATTCCGCGCGCTGGACGAACTGAAGCAGATGGCCGCCAAGTACGGCTAC
GACATCAGCCGTCCGGCCGCCAACGCCCGCGAAGCGGTGCAATGGGTGTACTTCGCCTACCTGGCCGCCGTCAAGGAACA
GAATGGCGCCGCCATGTCCTTCGGCCGCGTGTCCACCTTCCTGGACATCTACATCGAGCGCGACATCGCCGCCGGCGTGC
TGACCGAAGAGCAAGCCCAGGAAATGATCGACGACCTGGTGATCAAGCTGCGCATCGTCCGCTTCCTGCGCACGCCGGAA
TACGATCAGCTGTTCTCCGGCGACCCGACCTGGGTGACCGAATCCATCGGCGGCATGGGCCAGGACGGCCGCACCCTGGT
GACCAAGAACAGCTTCCGCTTCCTGAACACCCTGTACAACCTGGGCCCGGCGCCGGAGCCGAACCTGACCGTGCTGTGGT
CCACCCGCTTCCCGCAGGGCTTCAAGAACTTCTGCGCCAAGGTGTCCATCGATACCAGCGCCATCCAGTACGAAAACGAC
GATCTGATGCTGCCTTACTGGGGCGACGACTACGGCATCGCCTGCTGCGTGTCGGCGATGAAGATCGGCAAGCAGATGCA
GTTCTTCGGCGCCCGCGCCAACCTGGCCAAGGCCCTGCTGTACGCGATCAACGGCGGCCGCGACGAGAAGTCCGGCGCGC
TGGTGGCCAAGGGTTTCGAGCCGATCACCAGCGACGTGCTGACCTACGACGAGCTGATGCCCAAGTTCGAGAAGATGATG
GACTGGCTGGCCGCCACCTACGTCAAGGCGCTGAACTGCATCCACTACATGCACGACAAGTACGCCTACGAGCGCATCGA
AATGGCGCTGCACGACCGCGACATCGTCCGCACCATGGCTTGCGGCATCGCCGGCCTGTCCGTGGCCGCCGACTCGCTGT
CCGCCGTCAAGTTCGCCAAGGTGCACATCATCCGCAATGAGGAAGGCCTGGCCGTCGACTACAAGATCGAAGGCGACTAC
CCGGCTTACGGCAACAACGACGACCGCGTCGACGACATCGCCGTGTGGCTGACCCAGACCTTCATGGATCGCATCAAGGC
CCAGCCGTACTTCTACCGCGACTCCAAGCCGACCCAGTCGGTGCTGACCATCACCTCCAACGTGGTGTACGGCAAGAAGA
CCGGCAACACGCCGGACGGCCGCCGCGCCGGCGAACCGTTCTCCCCGGGCGCCAACCCGATGAACGGCCGCGACGTGAAG
GGCTTCGTCGCCGCGGGCGCCTCGGTGGCCAAGCTGCCGTACGACTCCGCGCTGGACGGCATCAGCTGGACCGCTTCGGC
CACGCCGGACGCGCTGGGCCACACCGCTGAAGAGCGCATCCTGAACCTGGCCAACTGCCTGGATGGCTTCTGCTCCGCGC
ACCCGACCGAGTTCAGCAGCGCCAACTGCACGCCGTTCGACTGCGAAGGCGAGCGCCACATCGCCGGCGGCGGCTTCCAC
GTCAACGTGAACGTGTTCAAGCGCGAAACCCTGCTGGACGCGATGGAGCATCCGGAACTGTACCCGCAGCTGACCATCCG
CGTGTCGGGCTACGCCGTGAACTTCATCAAGCTGACCCGCGAACAGCAACTGGACGTGATCAACCGCACCTTCCACGGCA
AGATGTAA

Upstream 100 bases:

>100_bases
GCGGGCTACTTGACTAAGATCAAGCGAAATTTTCGAATCCGAATGTACGATGTGTTCAATATCTAACACTTACTGGATCA
GCCAAGAAGGAGGCACCCAG

Downstream 100 bases:

>100_bases
GCTGATACTGGCCTCAAGCAGCAATGGGCGGGGCTTCCTTCCGGAACGCCCCGCCTTTTTCATGGATATACCGGAGATAC
GGCAATGACCCCACCCATCG

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MDAITQNATQANPWRDFVAGDWQSKVDVRNFIQLNYQPYEGDDSFLAGATERTKKLWDTLGELLKQERAKGVLDVSADRG
SSITAHDAGYIDQANEVIVGLQTDAPLKRAIMPNGGLRMVENGLEAFGFKLDPMIKEVWEKYRKSHNQGVFDVYTPEIMA
CRKSGVITGLPDAYGRGRIIGDYRRVALYGTDFLRAERQQVFHELDNATFTDDVMRLREELSEQFRALDELKQMAAKYGY
DISRPAANAREAVQWVYFAYLAAVKEQNGAAMSFGRVSTFLDIYIERDIAAGVLTEEQAQEMIDDLVIKLRIVRFLRTPE
YDQLFSGDPTWVTESIGGMGQDGRTLVTKNSFRFLNTLYNLGPAPEPNLTVLWSTRFPQGFKNFCAKVSIDTSAIQYEND
DLMLPYWGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGRDEKSGALVAKGFEPITSDVLTYDELMPKFEKMM
DWLAATYVKALNCIHYMHDKYAYERIEMALHDRDIVRTMACGIAGLSVAADSLSAVKFAKVHIIRNEEGLAVDYKIEGDY
PAYGNNDDRVDDIAVWLTQTFMDRIKAQPYFYRDSKPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFSPGANPMNGRDVK
GFVAAGASVAKLPYDSALDGISWTASATPDALGHTAEERILNLANCLDGFCSAHPTEFSSANCTPFDCEGERHIAGGGFH
VNVNVFKRETLLDAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHGKM

Sequences:

>Translated_775_residues
MDAITQNATQANPWRDFVAGDWQSKVDVRNFIQLNYQPYEGDDSFLAGATERTKKLWDTLGELLKQERAKGVLDVSADRG
SSITAHDAGYIDQANEVIVGLQTDAPLKRAIMPNGGLRMVENGLEAFGFKLDPMIKEVWEKYRKSHNQGVFDVYTPEIMA
CRKSGVITGLPDAYGRGRIIGDYRRVALYGTDFLRAERQQVFHELDNATFTDDVMRLREELSEQFRALDELKQMAAKYGY
DISRPAANAREAVQWVYFAYLAAVKEQNGAAMSFGRVSTFLDIYIERDIAAGVLTEEQAQEMIDDLVIKLRIVRFLRTPE
YDQLFSGDPTWVTESIGGMGQDGRTLVTKNSFRFLNTLYNLGPAPEPNLTVLWSTRFPQGFKNFCAKVSIDTSAIQYEND
DLMLPYWGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGRDEKSGALVAKGFEPITSDVLTYDELMPKFEKMM
DWLAATYVKALNCIHYMHDKYAYERIEMALHDRDIVRTMACGIAGLSVAADSLSAVKFAKVHIIRNEEGLAVDYKIEGDY
PAYGNNDDRVDDIAVWLTQTFMDRIKAQPYFYRDSKPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFSPGANPMNGRDVK
GFVAAGASVAKLPYDSALDGISWTASATPDALGHTAEERILNLANCLDGFCSAHPTEFSSANCTPFDCEGERHIAGGGFH
VNVNVFKRETLLDAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHGKM
>Mature_775_residues
MDAITQNATQANPWRDFVAGDWQSKVDVRNFIQLNYQPYEGDDSFLAGATERTKKLWDTLGELLKQERAKGVLDVSADRG
SSITAHDAGYIDQANEVIVGLQTDAPLKRAIMPNGGLRMVENGLEAFGFKLDPMIKEVWEKYRKSHNQGVFDVYTPEIMA
CRKSGVITGLPDAYGRGRIIGDYRRVALYGTDFLRAERQQVFHELDNATFTDDVMRLREELSEQFRALDELKQMAAKYGY
DISRPAANAREAVQWVYFAYLAAVKEQNGAAMSFGRVSTFLDIYIERDIAAGVLTEEQAQEMIDDLVIKLRIVRFLRTPE
YDQLFSGDPTWVTESIGGMGQDGRTLVTKNSFRFLNTLYNLGPAPEPNLTVLWSTRFPQGFKNFCAKVSIDTSAIQYEND
DLMLPYWGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGRDEKSGALVAKGFEPITSDVLTYDELMPKFEKMM
DWLAATYVKALNCIHYMHDKYAYERIEMALHDRDIVRTMACGIAGLSVAADSLSAVKFAKVHIIRNEEGLAVDYKIEGDY
PAYGNNDDRVDDIAVWLTQTFMDRIKAQPYFYRDSKPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFSPGANPMNGRDVK
GFVAAGASVAKLPYDSALDGISWTASATPDALGHTAEERILNLANCLDGFCSAHPTEFSSANCTPFDCEGERHIAGGGFH
VNVNVFKRETLLDAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHGKM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=775, Percent_Identity=64.7741935483871, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=777, Percent_Identity=60.8751608751609, Blast_Score=949, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=768, Percent_Identity=25.6510416666667, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790388, Length=694, Percent_Identity=24.3515850144092, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788933, Length=68, Percent_Identity=63.2352941176471, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 86637; Mature: 86637

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAITQNATQANPWRDFVAGDWQSKVDVRNFIQLNYQPYEGDDSFLAGATERTKKLWDTL
CCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHH
GELLKQERAKGVLDVSADRGSSITAHDAGYIDQANEVIVGLQTDAPLKRAIMPNGGLRMV
HHHHHHHHHCCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHCCCCCCHHHH
ENGLEAFGFKLDPMIKEVWEKYRKSHNQGVFDVYTPEIMACRKSGVITGLPDAYGRGRII
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCEECCCCCCCCCCEE
GDYRRVALYGTDFLRAERQQVFHELDNATFTDDVMRLREELSEQFRALDELKQMAAKYGY
ECEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DISRPAANAREAVQWVYFAYLAAVKEQNGAAMSFGRVSTFLDIYIERDIAAGVLTEEQAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
EMIDDLVIKLRIVRFLRTPEYDQLFSGDPTWVTESIGGMGQDGRTLVTKNSFRFLNTLYN
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHHH
LGPAPEPNLTVLWSTRFPQGFKNFCAKVSIDTSAIQYENDDLMLPYWGDDYGIACCVSAM
CCCCCCCCEEEEEECCCCHHHHHHHHEEEECCCEEEECCCCEEEEECCCCCHHHHHHHHH
KIGKQMQFFGARANLAKALLYAINGGRDEKSGALVAKGFEPITSDVLTYDELMPKFEKMM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
DWLAATYVKALNCIHYMHDKYAYERIEMALHDRDIVRTMACGIAGLSVAADSLSAVKFAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
VHIIRNEEGLAVDYKIEGDYPAYGNNDDRVDDIAVWLTQTFMDRIKAQPYFYRDSKPTQS
EEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHH
VLTITSNVVYGKKTGNTPDGRRAGEPFSPGANPMNGRDVKGFVAAGASVAKLPYDSALDG
HHEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCCHHHCCC
ISWTASATPDALGHTAEERILNLANCLDGFCSAHPTEFSSANCTPFDCEGERHIAGGGFH
CEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCEE
VNVNVFKRETLLDAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHGKM
EEEEEEHHHHHHHHHHCCCCCCEEEEEEECEEEEEEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDAITQNATQANPWRDFVAGDWQSKVDVRNFIQLNYQPYEGDDSFLAGATERTKKLWDTL
CCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHH
GELLKQERAKGVLDVSADRGSSITAHDAGYIDQANEVIVGLQTDAPLKRAIMPNGGLRMV
HHHHHHHHHCCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHCCCCCCHHHH
ENGLEAFGFKLDPMIKEVWEKYRKSHNQGVFDVYTPEIMACRKSGVITGLPDAYGRGRII
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCEECCCCCCCCCCEE
GDYRRVALYGTDFLRAERQQVFHELDNATFTDDVMRLREELSEQFRALDELKQMAAKYGY
ECEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DISRPAANAREAVQWVYFAYLAAVKEQNGAAMSFGRVSTFLDIYIERDIAAGVLTEEQAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
EMIDDLVIKLRIVRFLRTPEYDQLFSGDPTWVTESIGGMGQDGRTLVTKNSFRFLNTLYN
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHHH
LGPAPEPNLTVLWSTRFPQGFKNFCAKVSIDTSAIQYENDDLMLPYWGDDYGIACCVSAM
CCCCCCCCEEEEEECCCCHHHHHHHHEEEECCCEEEECCCCEEEEECCCCCHHHHHHHHH
KIGKQMQFFGARANLAKALLYAINGGRDEKSGALVAKGFEPITSDVLTYDELMPKFEKMM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
DWLAATYVKALNCIHYMHDKYAYERIEMALHDRDIVRTMACGIAGLSVAADSLSAVKFAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
VHIIRNEEGLAVDYKIEGDYPAYGNNDDRVDDIAVWLTQTFMDRIKAQPYFYRDSKPTQS
EEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHH
VLTITSNVVYGKKTGNTPDGRRAGEPFSPGANPMNGRDVKGFVAAGASVAKLPYDSALDG
HHEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCCHHHCCC
ISWTASATPDALGHTAEERILNLANCLDGFCSAHPTEFSSANCTPFDCEGERHIAGGGFH
CEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCEE
VNVNVFKRETLLDAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHGKM
EEEEEEHHHHHHHHHHCCCCCCEEEEEEECEEEEEEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA