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The map label for this gene is ywcJ [H]

Identifier: 34496866

GI number: 34496866

Start: 1487089

End: 1487871

Strand: Direct

Name: ywcJ [H]

Synonym: CV_1411

Alternate gene names: 34496866

Gene position: 1487089-1487871 (Clockwise)

Preceding gene: 34496848

Following gene: 34496867

Centisome position: 31.3

GC content: 64.62

Gene sequence:

>783_bases
ATGAGCCACTCCTCTACACTCGCCTACGTGGTCGACGAGGCAGCCAGCAAGCAAAAGGTGCTGCTGTCCGAGCCGGGCCG
CTACCTGCTCAGCGCCGCCATGGCCGGCGCGCTGATCGCCGTGGTGCTCGCGGTCAGCCTGAAACTGGGCCAGGTGTTCT
TCGCCGCCGGCGACGCCGGCTATTACATCGCCACGTCGGGTTTCTTCGGCGTGGCCCTGGTCAGCATCATCGTTTGCAAG
GTGGAGCTGTTCACCAGCAATGTGATGTATTTCACTGTCGGCCGACTGAGCGGCCGCTGCCGCACCTGCAATCTGCTGCG
CAGCTGGGCGATGGTATATATAGGCAACCTGATCGGCGTGCTGCTGTTCGTCGCGGTGCTGGCCGGCGCCGGCGGCATGG
GCGCGCTGCCGGCCAACCACCTGCTGTTCAATGTGGTGGAGCACAAGGTGCACGCGTCCACGATGGAAATCTTCTGGAAG
GGCGTGCTGTGCAACTGGGTGATCTGCCTGGCGGTGTGGGTGCCGATGCGGCTGGCCAACGAGGCGGCGCGCCTGCTGAT
CATCATGCTGCTGGTGTTCGTGTTCTTCTTCTCCGGTTTCGAGCACAGCATCGCCAACATGGCTTTCTTCGCGCTGGCCA
AGCTGCAGGGCATGGCCGCGCTGGAGTGGGGCGACATCCTGCACAATATGCTGCCGGCCACGCTGGGCAACATCGTCGGC
GGCGCCGTGGGCGTCGGCCTGGTGGTGTTCCGGCTGGAACGCCACACGCTGCAGGCGGTCTGA

Upstream 100 bases:

>100_bases
TAAAACGAACATAAAATTTTCGTGAAGTCGCATCGGATGCGAACGGCTGGGCCGGCACCGATCTGCGCCAGCTTACCCAC
TATTGAAGAGACGAAACGAA

Downstream 100 bases:

>100_bases
TAAGGCAGCAAGGCGTTTGCGCCGGTGAACGGCATGTAAATGTTCGATTTCGAATGTAGTCGGCGCGGTACATGGCGTAC
CGCGCCGATTTTGCTATAAA

Product: nitrite transporter

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSHSSTLAYVVDEAASKQKVLLSEPGRYLLSAAMAGALIAVVLAVSLKLGQVFFAAGDAGYYIATSGFFGVALVSIIVCK
VELFTSNVMYFTVGRLSGRCRTCNLLRSWAMVYIGNLIGVLLFVAVLAGAGGMGALPANHLLFNVVEHKVHASTMEIFWK
GVLCNWVICLAVWVPMRLANEAARLLIIMLLVFVFFFSGFEHSIANMAFFALAKLQGMAALEWGDILHNMLPATLGNIVG
GAVGVGLVVFRLERHTLQAV

Sequences:

>Translated_260_residues
MSHSSTLAYVVDEAASKQKVLLSEPGRYLLSAAMAGALIAVVLAVSLKLGQVFFAAGDAGYYIATSGFFGVALVSIIVCK
VELFTSNVMYFTVGRLSGRCRTCNLLRSWAMVYIGNLIGVLLFVAVLAGAGGMGALPANHLLFNVVEHKVHASTMEIFWK
GVLCNWVICLAVWVPMRLANEAARLLIIMLLVFVFFFSGFEHSIANMAFFALAKLQGMAALEWGDILHNMLPATLGNIVG
GAVGVGLVVFRLERHTLQAV
>Mature_259_residues
SHSSTLAYVVDEAASKQKVLLSEPGRYLLSAAMAGALIAVVLAVSLKLGQVFFAAGDAGYYIATSGFFGVALVSIIVCKV
ELFTSNVMYFTVGRLSGRCRTCNLLRSWAMVYIGNLIGVLLFVAVLAGAGGMGALPANHLLFNVVEHKVHASTMEIFWKG
VLCNWVICLAVWVPMRLANEAARLLIIMLLVFVFFFSGFEHSIANMAFFALAKLQGMAALEWGDILHNMLPATLGNIVGG
AVGVGLVVFRLERHTLQAV

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=246, Percent_Identity=34.5528455284553, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787132, Length=201, Percent_Identity=34.3283582089552, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788837, Length=204, Percent_Identity=28.4313725490196, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321779, Length=189, Percent_Identity=30.1587301587302, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 27933; Mature: 27802

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSSTLAYVVDEAASKQKVLLSEPGRYLLSAAMAGALIAVVLAVSLKLGQVFFAAGDAG
CCCCCHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
YYIATSGFFGVALVSIIVCKVELFTSNVMYFTVGRLSGRCRTCNLLRSWAMVYIGNLIGV
EEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LLFVAVLAGAGGMGALPANHLLFNVVEHKVHASTMEIFWKGVLCNWVICLAVWVPMRLAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAARLLIIMLLVFVFFFSGFEHSIANMAFFALAKLQGMAALEWGDILHNMLPATLGNIVG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GAVGVGLVVFRLERHTLQAV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHSSTLAYVVDEAASKQKVLLSEPGRYLLSAAMAGALIAVVLAVSLKLGQVFFAAGDAG
CCCCHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
YYIATSGFFGVALVSIIVCKVELFTSNVMYFTVGRLSGRCRTCNLLRSWAMVYIGNLIGV
EEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LLFVAVLAGAGGMGALPANHLLFNVVEHKVHASTMEIFWKGVLCNWVICLAVWVPMRLAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAARLLIIMLLVFVFFFSGFEHSIANMAFFALAKLQGMAALEWGDILHNMLPATLGNIVG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GAVGVGLVVFRLERHTLQAV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]