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The map label for this gene is pflA [H]

Identifier: 34496868

GI number: 34496868

Start: 1490555

End: 1491334

Strand: Direct

Name: pflA [H]

Synonym: CV_1413

Alternate gene names: 34496868

Gene position: 1490555-1491334 (Clockwise)

Preceding gene: 34496867

Following gene: 34496869

Centisome position: 31.37

GC content: 64.23

Gene sequence:

>780_bases
ATGACCCCACCCATCGCCGCAGACTACACCGCCCAGGCCGTCAGCACCGACACCATAGGCTATTTGCATTCCACCGAGAG
CGGCGCCGGCGTCGACGGCCCCGGCATGCGCTTCGTGTTTTTCGTTTCGGGCTGTCAATTCCGCTGCCTGTACTGCCACA
ACCCGGACACCTGGAAGCTGCACAATGGCCGCCAGGTCAGCGTCGAGCAGGCCTTGTCCGAAGTGGCGCCTTACGCCCGT
TTCCTCAAGTTCGCCGGCGGCGTCACCATTTCCGGCGGCGAGCCGCTGATGCAGCACGAATTCGTCGGCGAGCTCTTCCA
CGAGATCAAGCAGCGCTTCGGCCTGCACACCGCGCTGGACACCCAGGGTTTCCTGCACGAGCGCGTGTCTGATGCGTGGT
TCGACGACGTCGATCTGGTGATGCTGGACATCAAGCATTCCGATCCGGAAAAGTACCAGGCGCTGACCGGCCAGCCGCTG
CAGCCGACGCTGGATTTCGCCCTGCGCCTGAAGCGGCTGCGGAAAAAGATGTGGATACGCTACGTGCTGGTGCCCGGCCT
GACCGACGGCGACGCCGACATCGAGAAGCTGGCGGATTTCGCCGCTTCGCTTGGCGACGTGGTGGAGCGGGTGGAGGTGC
TGCCTTTCCATCAACTGGGCAAGGACAAGTGGGCGCAGCTGGGCATGGAGTACCAGTTGAACGACACCCCGATTCCCACC
GCGGAGCAGGCCGCGCATGCGCGCGCGCTGTTCGCCAGCCGCGGGCTGAAAGTTACCTGA

Upstream 100 bases:

>100_bases
CCACGGCAAGATGTAAGCTGATACTGGCCTCAAGCAGCAATGGGCGGGGCTTCCTTCCGGAACGCCCCGCCTTTTTCATG
GATATACCGGAGATACGGCA

Downstream 100 bases:

>100_bases
GTTTCGGTTGATCCATGCTCCCAAACCGCCGCGCGCCTTGCGCCGGCGGTTTTCGCGTTTTTGGGGCACCTTGGCCGCTG
GGCATGGTCTGCAGAATGGA

Product: pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTPPIAADYTAQAVSTDTIGYLHSTESGAGVDGPGMRFVFFVSGCQFRCLYCHNPDTWKLHNGRQVSVEQALSEVAPYAR
FLKFAGGVTISGGEPLMQHEFVGELFHEIKQRFGLHTALDTQGFLHERVSDAWFDDVDLVMLDIKHSDPEKYQALTGQPL
QPTLDFALRLKRLRKKMWIRYVLVPGLTDGDADIEKLADFAASLGDVVERVEVLPFHQLGKDKWAQLGMEYQLNDTPIPT
AEQAAHARALFASRGLKVT

Sequences:

>Translated_259_residues
MTPPIAADYTAQAVSTDTIGYLHSTESGAGVDGPGMRFVFFVSGCQFRCLYCHNPDTWKLHNGRQVSVEQALSEVAPYAR
FLKFAGGVTISGGEPLMQHEFVGELFHEIKQRFGLHTALDTQGFLHERVSDAWFDDVDLVMLDIKHSDPEKYQALTGQPL
QPTLDFALRLKRLRKKMWIRYVLVPGLTDGDADIEKLADFAASLGDVVERVEVLPFHQLGKDKWAQLGMEYQLNDTPIPT
AEQAAHARALFASRGLKVT
>Mature_258_residues
TPPIAADYTAQAVSTDTIGYLHSTESGAGVDGPGMRFVFFVSGCQFRCLYCHNPDTWKLHNGRQVSVEQALSEVAPYARF
LKFAGGVTISGGEPLMQHEFVGELFHEIKQRFGLHTALDTQGFLHERVSDAWFDDVDLVMLDIKHSDPEKYQALTGQPLQ
PTLDFALRLKRLRKKMWIRYVLVPGLTDGDADIEKLADFAASLGDVVERVEVLPFHQLGKDKWAQLGMEYQLNDTPIPTA
EQAAHARALFASRGLKVT

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=244, Percent_Identity=44.672131147541, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1790389, Length=292, Percent_Identity=25.3424657534247, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790839, Length=235, Percent_Identity=29.7872340425532, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI226510931, Length=166, Percent_Identity=27.710843373494, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28878; Mature: 28747

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPIAADYTAQAVSTDTIGYLHSTESGAGVDGPGMRFVFFVSGCQFRCLYCHNPDTWKL
CCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEE
HNGRQVSVEQALSEVAPYARFLKFAGGVTISGGEPLMQHEFVGELFHEIKQRFGLHTALD
CCCCEECHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHC
TQGFLHERVSDAWFDDVDLVMLDIKHSDPEKYQALTGQPLQPTLDFALRLKRLRKKMWIR
CHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEE
YVLVPGLTDGDADIEKLADFAASLGDVVERVEVLPFHQLGKDKWAQLGMEYQLNDTPIPT
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHCCCEEEECCCCCCC
AEQAAHARALFASRGLKVT
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TPPIAADYTAQAVSTDTIGYLHSTESGAGVDGPGMRFVFFVSGCQFRCLYCHNPDTWKL
CCCCCCCCHHHHHCCCHHHEEECCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEE
HNGRQVSVEQALSEVAPYARFLKFAGGVTISGGEPLMQHEFVGELFHEIKQRFGLHTALD
CCCCEECHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHC
TQGFLHERVSDAWFDDVDLVMLDIKHSDPEKYQALTGQPLQPTLDFALRLKRLRKKMWIR
CHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEE
YVLVPGLTDGDADIEKLADFAASLGDVVERVEVLPFHQLGKDKWAQLGMEYQLNDTPIPT
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHCCCEEEECCCCCCC
AEQAAHARALFASRGLKVT
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]