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The map label for this gene is ecpD [H]

Identifier: 34496750

GI number: 34496750

Start: 1362393

End: 1363142

Strand: Direct

Name: ecpD [H]

Synonym: CV_1295

Alternate gene names: 34496750

Gene position: 1362393-1363142 (Clockwise)

Preceding gene: 34496749

Following gene: 34496751

Centisome position: 28.68

GC content: 62.67

Gene sequence:

>750_bases
ATGAAACAGGCAGTATGGCGAGGAGCGCTTGCTCTTGCGATTGCGGGATCGGCATGGACCGGCGCGGCTTCGGCCGGCGT
GGTGGTGTCCGGCACCCGCGTCGTGTACAAGGCCGAGGACCGCGAGGTGACCGTCAAGGTCAACAATCCCGGCAAGGAGC
CTTCCTTGGTGCAGGTGTGGGCCGACAAGGGCAATGAGAAGTCCGTGCCCGATACCGCCGATGCGCCTTTCCTGATCATG
CCGCCGATTTTCCGTGTCGATCCTGCCAAGGGCCAGACCCTGAGACTGACCTTCACCGGCGCGGATCTGCCCAAGGAACG
CGAGTCGGTGTTCTGGCTGAACGTGCTGGATGTGCCGCCGCTGCCCAAGGCCGCGAGCGAGCAGCGCAATTTCATGCAAG
TCGCTTTCCGTTCTCGCATCAAGCTGTTCTATCGCCCCGTCGGCCTGCCGGGCACGCCGGACGAAGCGGCCGACAAGCTT
TCCTGGTCTTTGCTGCCGCAGGCCGGCGGCAAGGGCTACATGCTGCGAGCCTCCAATCAGGCCGCGTACCACGTTTCGCT
GAACCGGGCGGTTCTGCTGGTGGGCAAACATGAATATGAAACCGACGGCGGCATGGTTCCGCCGGGCGGCAGCCACGATT
TCGCGCTGAAGGATCTGAAACTCAAGCCGGAAGGCGCTTTGAAGCTCAACTACGAGAGCATCAATGATTACGGTGCCCCG
GTCGCGCACAGCATATCCCTCGCTCACTGA

Upstream 100 bases:

>100_bases
CGTTCAGTACTCGATGACCTACCAATAAAACGGCGTCATGCGGCAGGCGGCTCAGCGTGAGCCGCCTGCCGATCTTTTTT
GCATGGAGGCGGGGTTGGAA

Downstream 100 bases:

>100_bases
TCATCCCGCTCGAGCCAGCCTGGCCCGCGCGCACCCGCAGGACTCAACCAGCCATGACGCACATGACGACACGACAGCCC
GCTCGACGGTGGGATCTCAG

Product: chaperone protein ecpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKQAVWRGALALAIAGSAWTGAASAGVVVSGTRVVYKAEDREVTVKVNNPGKEPSLVQVWADKGNEKSVPDTADAPFLIM
PPIFRVDPAKGQTLRLTFTGADLPKERESVFWLNVLDVPPLPKAASEQRNFMQVAFRSRIKLFYRPVGLPGTPDEAADKL
SWSLLPQAGGKGYMLRASNQAAYHVSLNRAVLLVGKHEYETDGGMVPPGGSHDFALKDLKLKPEGALKLNYESINDYGAP
VAHSISLAH

Sequences:

>Translated_249_residues
MKQAVWRGALALAIAGSAWTGAASAGVVVSGTRVVYKAEDREVTVKVNNPGKEPSLVQVWADKGNEKSVPDTADAPFLIM
PPIFRVDPAKGQTLRLTFTGADLPKERESVFWLNVLDVPPLPKAASEQRNFMQVAFRSRIKLFYRPVGLPGTPDEAADKL
SWSLLPQAGGKGYMLRASNQAAYHVSLNRAVLLVGKHEYETDGGMVPPGGSHDFALKDLKLKPEGALKLNYESINDYGAP
VAHSISLAH
>Mature_249_residues
MKQAVWRGALALAIAGSAWTGAASAGVVVSGTRVVYKAEDREVTVKVNNPGKEPSLVQVWADKGNEKSVPDTADAPFLIM
PPIFRVDPAKGQTLRLTFTGADLPKERESVFWLNVLDVPPLPKAASEQRNFMQVAFRSRIKLFYRPVGLPGTPDEAADKL
SWSLLPQAGGKGYMLRASNQAAYHVSLNRAVLLVGKHEYETDGGMVPPGGSHDFALKDLKLKPEGALKLNYESINDYGAP
VAHSISLAH

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=230, Percent_Identity=39.5652173913043, Blast_Score=170, Evalue=6e-44,
Organism=Escherichia coli, GI1788428, Length=239, Percent_Identity=32.2175732217573, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1789532, Length=232, Percent_Identity=33.6206896551724, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790771, Length=221, Percent_Identity=33.9366515837104, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786743, Length=230, Percent_Identity=33.0434782608696, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787171, Length=248, Percent_Identity=30.6451612903226, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786936, Length=168, Percent_Identity=32.1428571428571, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082203, Length=265, Percent_Identity=24.9056603773585, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87081806, Length=224, Percent_Identity=31.6964285714286, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788677, Length=188, Percent_Identity=30.3191489361702, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26900; Mature: 26900

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAVWRGALALAIAGSAWTGAASAGVVVSGTRVVYKAEDREVTVKVNNPGKEPSLVQVW
CCCCHHHCEEEEEEECCCCCCCCCCCEEEECCEEEEEECCCEEEEEECCCCCCCCEEEEE
ADKGNEKSVPDTADAPFLIMPPIFRVDPAKGQTLRLTFTGADLPKERESVFWLNVLDVPP
ECCCCCCCCCCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCC
LPKAASEQRNFMQVAFRSRIKLFYRPVGLPGTPDEAADKLSWSLLPQAGGKGYMLRASNQ
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHCEEEECCCCCCCEEEEEECCC
AAYHVSLNRAVLLVGKHEYETDGGMVPPGGSHDFALKDLKLKPEGALKLNYESINDYGAP
EEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCC
VAHSISLAH
CEEEEEECC
>Mature Secondary Structure
MKQAVWRGALALAIAGSAWTGAASAGVVVSGTRVVYKAEDREVTVKVNNPGKEPSLVQVW
CCCCHHHCEEEEEEECCCCCCCCCCCEEEECCEEEEEECCCEEEEEECCCCCCCCEEEEE
ADKGNEKSVPDTADAPFLIMPPIFRVDPAKGQTLRLTFTGADLPKERESVFWLNVLDVPP
ECCCCCCCCCCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCC
LPKAASEQRNFMQVAFRSRIKLFYRPVGLPGTPDEAADKLSWSLLPQAGGKGYMLRASNQ
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHCEEEECCCCCCCEEEEEECCC
AAYHVSLNRAVLLVGKHEYETDGGMVPPGGSHDFALKDLKLKPEGALKLNYESINDYGAP
EEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCC
VAHSISLAH
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]